The study was conducted to evaluate efficiency of artificial insemination (AI) service and constraints of AI service selected Districts of Harar National Region State, Ethiopia. A total of 320 dairy cows were randomly selected from six districts of Harari region; Jin’Eala, Shenkor, Hakim, Dire-Teyara, Aboker, Sofi. Data on dairy cattle management, artificial insemination (AI) service efficiency and constraints of AI service were generated using structured and semi-structured questionnaire. Forty three straws of frozen semen was sampled from the regional AI service centre, thawed and evaluated at the region level for its quality (motility, viability and morphology) following standard procedures. The overall mean percentage of frozen semen motility at regional level was 49.6. Semen production period (batch) and Breed (exotic blood level) had no effect on frozen semen motility. The mean percentage of viable (live) spermatozoa was 58.3. Sperm viability was not significantly influenced by Semen production period (batch) and, breed (exotic blood level). The overall mean percentages of major and minor sperm morphological defects were 5.6 and 21.8, respectively. Frozen sperm major morphological defects were significantly affected ( p < 0.05) by Semen production period (batch), however sire breed has no effect ( p > 0.05) on frozen sperm major morphological defects. Frozen sperm minor morphological defects were not significantly affected ( P > 0.05) by both semen production period (batch) and sire breed (exotic blood level). The mean number of service per conception (NSC) in the current study was found to be 1.6. It was not significantly affected by previous season of calving, previous year of calving and parity. Non-return rate value of Harari region AI service center was 48.1%. Major constraints of AI service delivery system in the study area were feed shortage, problem in heat detection, service charge for AI, distance from AI service centre and husbandry problem. Additional shortage of input for AI service activity particularly semen and liquid nitrogen was reported as constraint of artificial insemination service in the study area, which interrupt smooth delivery of AI service.
Ethiopia possesses the largest livestock population compared to any country in Africa, nevertheless its productivity remains marginal [
In Ethiopia almost all data used to evaluate breeding works so far are from government ranches or research stations. Besides, there were few field studies made to evaluate efficiency of artificial insemination (AI) service [
The study was conducted in 6 districts of Harari region; Jin’Eala, Shenkor, Hakim, Dire-Teyara, Aboker, Sofi. The districts are among the nine districts of Harari national regional State eastern Ethiopia. The Harari region is one of the nine administrative regions of Ethiopia. Harari National Regional State is located at a distance of 525 km eastern of Addis Ababa. The Harari region lies between latitude 9˚24'N and 9˚42'03"E and 42˚16'E longitude. The Harari region has a wet tropical and receives an annual rainfall between 600 and 900 mm in a bimodal pattern. The bimodal pattern is characterized by a short rainy season that occurs between March and April and a long rainy season that occurs between July and September [
The template is used to format your paper and style the text. Laboratory examinations of frozen semen were conducted to estimate frozen sperm motility, viability (live and dead cell count) and morphological defects. Besides, a cross-sectional type of study was carried out to assess, organization of artificial insemination (AI) service, methods of AI service delivery, price of AI service and challenges of AI service under smallholder dairy production system in the study areas. A semi-structured survey questionnaire, farm inspection and secondary data sources were used to collect the required information.
A total of 43 straws of frozen semen were subjected to laboratory examination to analyze frozen semen quality. The sample size (43) is an appropriate range, since
the range has sufficient statistical power and help to come up with correct decision on this particular research. The sample size is applicable to make more accurate decision and to minimize errors of the parameter estimate in the current study. The straws were randomly collected and examined to estimate frozen sperm motility, viability (live and dead cell count) and morphological defects. The frozen semen was composed of two breeds (100% Holstein Friesian, and 75% Holstein Friesian and 25% Boran) and three production batches (B1 = batch code 15/163 and production date 05/05/2015; B2 = batch code 15/101 and production date 07/05/2015; B3 = batch code 15/100 and production date 12/05/2015). Data on sperm quality at the site of production and processing was collected from record book at National AI center. Thus, loss of sperm quality was estimated as the difference between the two measurements. The laboratory examination procedures of semen were done in accordance with the procedures provided by [
Sixty sample dairy producers (320 dairy cows) were selected from selected districts of Harari region. Sampled dairy producers are households who are living in the urban part of the study area, and provide very little or limited specialized inputs to their dairy enterprise. They sell fresh milk on a daily basis to private consumers. Excess milk is processed into butter and a local cottage cheese, ayib and sold in local markets, and used for domestic consumption. The system involves special inputs like artificial insemination and little supplementary feeds to roughages. Primary information was collected from targets household using structured and semi-structured questionnaires, observation and direct measurements. The survey was carried out across the milk shed to collect primary data using structured and semi-structured questionnaires, which were developed and pre-tested to check appropriateness and clarity of the questions. During the interview process, every respondent included in the study was briefed about the objective of the study before presenting the actual questions. Some of the information collected through interview was supported by observation.
The collected data were computed using descriptive statistics. The General Liner Model procedure [
Model I: Analysis of frozen semen quality
yijk = μ+ Bj+ Pk + ejk
where: yjk = frozen semen quality (sperm motility, viability and morphology); μ = Overall mean; Bj = Effects of jth Sire breed (100% Holstein Friesian, and 75% Holstein Friesian and 25% Boran); Pk = Effect of kth Semen Production Period (B1= batch code 15/163 and production date 05/05/2015; B2 = batch code 15/101 and production date 07/05/2015; B3= batch code 15/100 and production date 12/05/2015); ejk = residual.
Model II: Analysis of number of service per conception (NSC)
yjkl= μ+ Gj + Sk+ Pl + ejkl
where: yjkl = trait (NSC); μ = over all mean; Gj = effect of jth previous calving year (2013, 2014, 2015); Sk = effect of kth previous calving season (short rainy season, dry season, long rainy season); Pl = effect of lth parity of dairy cows; ejkl = random error.
The values of fresh sperm individual progressive motility was obtained from national artificial insemination center (NAIC), where semen is collected processed and packed. The measurement was conducted immediately after the semen is collected. However, frozen sperm individual progressive motility was measured both at national artificial insemination center (NAIC) at the end of semen processing procedures and at the regional AI service center after transportation and further storage. Fresh semen individual progressive motility for all samples at the time of collection and processing at NAIC was 80% (
Batch | Individual progressive motility (%) | |||
---|---|---|---|---|
Fresh semen at NAIC | Frozen semen at NAIC | Frozen semen at district | Motility loss | |
B1 | 80 | 58 | 47.8 ± 2.26 | 10.2 |
B2 | 80 | 63 | 49.1 ± 1.23 | 13.9 |
B3 | 80 | 55 | 51.3 ± 1.07 | 3.7 |
80 | 58.7 | 49.4 | 9.3 |
N = number of observation; (B1 = batch code 15/163 and production date 05/05/2015; B2 = batch code 15/101 and production date 07/05/2015; B3 = batch code 15/100 and production date 12/05/2015).
Sperm individual motility can be defined as the percentage of all moving sperm in a semen sample. It is measured by placing a drop of semen on a slide using a light microscope under a cover slide at 400 x magnifications (scored at 10% unit intervals). However viability is measurement of live and dead spermatozoa in a semen sample. Viable in its most basic sense means “live”. Viability is measured using a light field microscope with an oil immersion lens at 1000X magnification, after letting the semen sample to be stained with eosin/nigrosin solution. Both Sperm individual Motility and Viability helps to figure out semen quality and they are presented in percentage. The overall regional mean of frozen semen progressive individual motility in the current study at was 49.6% (
The overall mean frozen semen viability at regional level was 58.3 (
Variables | Frozen semen progressive motility and viability (%) | ||
---|---|---|---|
N | Motility (LSM ± SE) | Viability (LSM ± SE) | |
Overall mean | 43 | 49.6 ± 0.86 | 58.3 ± 0.77 |
Batch (production period) | ns | ns | |
B1 | 12 | 47.8 ± 2.26 | 56.6 ± 2.04 |
B2 | 14 | 49.1 ± 1.23 | 59.6 ± 0.78 |
B3 | 17 | 51.3 ± 1.07 | 58.4 ± 1.14 |
Sire breed | ns | ns | |
75% HF | 21 | 48.9 ± 1.18 | 58.5 ± 1.13 |
100% HF | 22 | 50.3 ± 1.27 | 58.1 ± 1.06 |
N = number of observation; (B1 = batch code 15/163 and production date 05/05/2015; B2 = batch code 15/101 and production date 07/05/2015; B3 = batch code 15/100 and production date 12/05/2015); ** = P < 0.01; *P < 0.05; ns = P > 0.05.
not significantly (P > 0.05) affected by batch (production period) and sire breed. However, sperm viability recorded in this study, irrespective of the different effects, was higher than the minimum threshold of 50%, below which conception was negatively influenced.
The overall mean morphological defects of frozen semen at regional level were 27.4% (
The overall mean of major and minor morphological defect of frozen spermatozoa in the current study was 5.6% and 21.8%, respectively (
Variables | Morphological defects (%) | ||
---|---|---|---|
Overall defect (LSM ± SE) | Major defect (LSM ± SE) | Minor defect (LSM ± SE) | |
Overall mean | 27.4 ± 0.85 | 5.6 ± 0.23 | 21.8 ± 0.79 |
Batch | ns | * | ns |
B1 | 28.4 ± 1.75 | 6.1 ± 0.36 a | 22.3 ± 1.74 |
B2 | 26.4 ± 1.48 | 4.7 ± 0.49 b | 21.7 ± 1.39 |
B3 | 27.5 ± 1.36 | 5.9 ± 0.30 c | 21.6 ± 1.18 |
Breed | ns | ns | ns |
75% HF | 28.3 ± 1.30 | 5.6 ± 0.27 | 22.7 ± 1.25 |
100% HF | 26.5 ± 1.13 | 5.6 ± 0.39 | 21.0 ± 0.98 |
Mean normal spermatozoa | 72.6 |
N = number of observation; (B1 = batch code 15/163 and production date 05/05/2015; B2 = batch code 15/101 and production date 07/05/2015; B3 = batch code 15/100 and production date 12/05/2015).; *** = P < 0.001; ** = P < 0.01; * = P < 0.05; ns = P > 0.05, means non-significant; means with the same superscript are not significantly different (P > 0.05).
The current study indicated batch (semen production date) had significant effect (P < 0.05) on frozen semen major morphological defects. This variability might be due to differences in semen handling practices, transportation and freezing facilities. Pear shaped head, small abnormal head, detached abnormal head, acrosome defects, middle piece defects, tail stump, proximal droplet, strongly folded tail and narrow at the base are some of the common major sperm morphological defects recorded in the current study.
The percent of minor morphological defects recorded in this study was lower than 46.1% reported by [
The mean frozen semen head morphological defect in the current stud was 4.7%. The most common head defects in this study were pear shaped head, narrow at the base, abnormal counter, abaxial implantation, detached normal and abnormal head, giant broad head and acrosome defects.
The overall mean sperm mid-piece defect and tail morphological defects were 8.1% and 14.6% respectively. The result obtained in current study for head morphological defect, morphological defects of mid-piece and tail morphological defects were higher than 4.6%, 7.8% and 13.2% for head morphological defect, morphological defects of mid-piece and tail morphological defects respectively, which is indicated by [
In general, the overall mean frozen sperm morphological defects (27.4%) are consistent with what has been recommended (25%) for better conception rate.
Variables | Morphological defects (%) | ||
---|---|---|---|
Head-defects (LSM ± SE) | Mid-piece-defect (LSM ± SE) | Tail-defect (LSM ± SE) | |
Overall mean | 4.7 ± 0.17 | 8.1 ± 0.30 | 14.6 ± 0.84 |
Batch | ns | ns | ns |
B1 | 4.7 ± 0.32 | 7.8 ± 0.60 | 15.9 ± 1.66 |
B2 | 4.4 ± 0.27 | 8.2 ± 0.56 | 13.8 ± 1.40 |
B3 | 5.0 ± 0.29 | 8.2 ± 0.46 | 14.2 ± 1.40 |
Breed | ns | ns | ns |
75% HF | 4.9 ± 0.25 | 7.9 ± 0.38 | 15.3 ± 1.16 |
100% HF | 4.5 ± 0.23 | 8.1 ± 0.47 | 13.8 ± 1.22 |
N = number of straw per sample; HF = Holstein Friesian; (B1 = batch code 15/163 and production date 05/05/2015; B2 = batch code 15/101 and production date 07/05/2015; B3 = batch code 15/100 and production date 12/05/2015); ns = non-significant; means with the same superscript are not significantly different (P > 0.05).
In this study, the proportion of morphologically abnormal frozen spermatozoa was slightly higher (27.4%) as compared to the maximum recommended level (25%) for better conception rate. The mean percent sperm morphological head defects recorded in the current study was less than the recommended maximum value of 10%, above which conception rate might be affected.
The mean number of service per conception in this study was 1.60 (
The result in the current study indicated that previous calving year had no significant effect (P > 0.05) on number of service per conception. Similar results were reported by Belayneh et al. (2013) and [
Variables | Number of Service per Conception | |
---|---|---|
N | LMS ± SE NSC | |
Over all Mean | 320 | 1.60 ± 0.03 |
Previous calving season | ns | |
Short rainy season | 80 | 1.55 ± 0.07 |
Long rainy season | 116 | 1.61 ± 0.05 |
Dry season | 124 | 1.65 ± 0.06 |
Previous calving year | ns | |
2013 | 63 | 1.60 ± 0.10 |
2014 | 126 | 1.64 ± 0.06 |
2015 | 131 | 1.58 ± 0.05 |
Parity | ns | |
2 | 87 | 1.62 ± 0.06 |
3 | 127 | 1.69 ± 0.06 |
4 | 72 | 1.55 ± 0.07 |
5 | 34 | 1.41 ± 0.09 |
N = number of observations, NSC = number of service per conception; ** = P < 0.01; *** = P < 0.001, ns = non-significant; means with the same superscript are not significantly different (P > 0.05).
The same author also indicated that the variation was due to provision of bonus for each successful insemination they achieved and in later years this incentive was ceased and again reinitiated.
Number of services per conception was not significantly affected (p > 0.05) by previous calving season and parity. The finding was in general agreement with the study reported by [
The overall mean non-return rate to first inseminations in the current study was 48.1% (
The most common AI service delivery system in the study area is mobile service delivery systems. It is a system in which AI technicians use motorcycles in order to visit smallholder dairy farms when informed by the owner through telephone on in person at their office. Generally there are two types of AI delivery systems in the study area: stationed and mobile service delivery systems. In stationery AI service delivery system, inseminators are stationed individually and farmers bring their cows to the insemination point. This is mostly practiced in the study area, and has been recommended as reported by [
Feed shortage, problem in heat detection, service charge for AI, distance from AI center and husbandry problem were the major constraints of AI service delivery system in the study area (
Overall N | 1st insn | 2nd insn | 3rd insn | NRR | ||||
---|---|---|---|---|---|---|---|---|
N | % | N | % | N | % | |||
Overall Performance | 320 | 154 | 48.1 | 114 | 44.1 | 20 | 6.3 | 48.1 |
N = Number of observation; NRR = non return rate; insn = insemination.
Variable (Constraint) | Proportion (%) | |
---|---|---|
N | Percentage* | |
Feed shortage | 56 | 93.3 |
Problem in heat detection | 49 | 81.7 |
Service charge for AI | 43 | 71.7 |
Distance from AI center | 13 | 21.7 |
Husbandry Problem | 8 | 13.3 |
N = number of observation; * = multiple responses were possible.
that heat detection, AI technicians’ efficiency and fertility level of the herd was the most severe problems of AI delivery system. Additional shortage of input for AI activity particularly semen and liquid nitrogen was reported as constraint of artificial insemination service in the current study, which have an adverse effect on semen quality and reproductive performance of dairy cows.
In the current study, about 61.67% of AI beneficiary households (close to half) complained that they had a problem with regard to sex of the calves when they used artificial insemination. Thus, among these 61.67% households, about 83.78% reported that the tendency was higher for male sex than females. Since dairy farms are highly dependent on females for replacement and expansion purpose and thereby increase milk production, the current complaint of having more male calf than females deserves further research.
Generally, the overall observed efficiency of artificial insemination service under smallholder dairy cow’s management system in the study area was fairly good. Hence, coverage of the AI service and the ongoing activities to improve and expand provision of liquid nitrogen and semen with appropriate exotic blood level at smallholder level in the area should be encouraged. However, shortage of feed, inadequate heat detection practices, service charge for AI, long distance from AI center, and poor management of animals are identified as major constraints of AI services in the current study.
The author is thankful to Haramaya University for funding the research work; artificial insemination technicians and dairy farmers of Jin’Eala, Shenkor, Hakim, Dire-Teyara, Aboker, Sofi districts and Harari Region Office of Livestock Resource and Fisheries for providing all necessary information/facilities during the course of the study.
Engidawork, B. (2018) Artificial Insemination Service Efficiency and Constraints of Artificial Insemination Service in Selected Districts of Harari National Regional State, Ethiopia. Open Journal of Animal Sciences, 8, 239-251. https://doi.org/10.4236/ojas.2018.83018