Chronic social defeat stress induces diverse effects in mice of different strains and even in animals of the same strain. This paper aims to study the effect of repeated social defeats and, for contrast, repeated aggression in daily agonistic interactions on the behaviors of CD-1 male mice. The behavior of animals that have the same winning and losing track record during 3, 10, 21 days is studied in different tests. The level of aggressiveness, as estimated by the number and total time of attacks, decreases; nevertheless, direct and indirect forms of aggression demonstrated by the aggressive mice (winners) remain significantly high. The number of stereotypic behaviors (rotations and jumps) and total time of digging behaviors are significantly increased in the winners after 21 days compared to 3 and 10 days of intermale confrontations. Among the losers, chronic experience of social defeats is accompanied by the development of pronounced anxiety and a depression-like state estimated by the elevated plus-maze and the Porsolt test scores, respectively. Both groups of male mice with alternative social behaviors demonstrate abnormal locomotor and exploratory behaviors in the open-field test. This phenomenon may be viewed as hyperactivity developed under chronic agonistic interactions and specific for the outbred CD-1 mice. We believe that these animals may be potentially used for modeling the key symptoms of bipolar disorder.
Chronic social defeat stress (CSDS) is an etiologic factor which is used for the production of depression-like states in mice [
Our first behavioral observations of outbred CD-1 mice allowed us to suggest that chronic agonistic interactions may lead to the development of psychopathologies other than in the above-mentioned inbred mice. CD-1 mice are widely used in research as a common outbred stock in general multipurpose models, safety and efficacy testing, aging, surgical and pseudopregnancy models [
The CD-1 male mice maintained at the conventional Animal Facility of the Institute of Cytology and Genetics, SD RAS (Novosibirsk, Russia) were used. The animals were housed under standard conditions (12:12 hr light/ dark regime, switch-on at 8.00 a.m.; food (pellets) and water available ad libitum). Experiments were performed on 10 - 12 week-old mice, 38 - 40 g of body weight.
All procedures were in accordance with the European Communities Council Directive of November 24, 1986 (86/609/EEC). The study was approved by Scientific Council N 9 of the Institute of Cytology and Genetics SD RAS of March, 24, 2010, N 613.
Prolonged negative and positive social experiences (defeats and victories) in male mice were induced by daily agonistic interactions [
After 5 mins of activation, partitions were removed and the behavior of animals in the agonistic interactions test was video-recorded for 10 mins during the encounter, and the data were documented. Regarding behavioral domains, the following components of agonistic behaviors were observed in the aggressive mice in relation to the losers and registered during the third (Win3), tenth (Win10) and twentieth first (Win21) days during the 10-min test: 1) Attack: latency of the first attack, attacking, biting and chasing; 2) Aggressive grooming: the winner mounting the loser’s back, holding it down and spending much time licking and nibbling at the scruff of the loser’s neck (during aggressive grooming, the loser appeared fully immobilized or sometimes stretched out its neck and then again froze under the winner); 3) Digging: digging up and scattering the sawdust on the loser’s territory (kick-digs: pulling the sawdust forward with the forepaws; push-digs: pushing the sawdust backward with the hind paws); 4) Hostile behavior: the total time spent attacking, aggressively grooming and digging; 5) Self- grooming: body care activities (fur licking, head washing, and nose washing); 6) Threats: tail rattling, jumps and rotations (quick and sharp turns). The total time and/or number of events, as well as the fraction of animals demonstrating aggressive grooming and threats, were also measured.
The behavioral domain analysis in defeated mice included the following components of submissive behavior during the third (Los3), tenth (Los10) and twentieth first (Los21) days of agonistic interaction with the winners during the 10-min test: l) Active defense: sideways and upright postures when under the attacks of the winner; 2) Escape: fleeing from the winners; 3) Immobility or passive defense behavior: freezing, full submission (posture “on the back”), immobility evoked by the winners’ attacks or aggressive grooming; 4) Rearing in search for a way out of the cage; 5) Approaches to the winners; 6) Waiting: sitting at the corner or at the cage’s wall and observing the winner’s movements. The total time or/and number of events were also measured. To eliminate the differences in a partner’s behavior, the percentage of each posture’s duration of the total time of defensive behavior in the losers or aggressive behavior in the winners were calculated. The Observer XT, Version 7.0 (Noldus Information Technology, Wageningen, Netherlands) were used for analysis.
The partition test [
The elevated plus-maze test [
The open-field test [
In the Porsolt test [
One-way ANOVA was used to reveal any significant influence of the factors “experience of aggression” (days) (Win3, Win10, Win21) or “experience of defeat” (days) (Los3, Los10, Los21) on the mice behavior in the agonistic interactions and partition tests. Additionally, one-way ANOVA was used to reveal any significant influence of the factor “groups” (control, Win21, Los21) on the different forms of mice behavior in the plus-maze and open-field tests. A post hoc pair wise comparison of the groups was made using the Bonferroni or LSD tests. For comparison of the behaviors (Win3 vs. Los3; Win10 vs. Los10; and Win21 vs. Los21 in the partition test, and the controls vs. Los21 in the Porsolt test), the t-test was used. The percentage of animals demonstrating aggressive groomings and threats in the agonistic interactions test was analyzed using the chi-square test. The Spearman correlational analysis was performed within every group of the winners in the agonistic interactions and partition tests. The behavioral structure was estimated in the 2nd, 10th and 21th tests as the percentage of the total time of the aggressive grooming, attacking, digging and self-grooming behavior for the winners, and as the percentage of the total time of active defense, waiting, escape and immobility in the losers. The data were reported as mean ± SEM (n = 10 - 14 per group).
On day one, all male mice demonstrated agonistic behavior in 100% of cages: 8 pairs fought on par. On day two, 2 pairs fought on par, and on day three, 1 pair did. Three days later, the status of the winner and the loser became obvious. On the following days, throughout the entire experimental period, none of the males stopped fighting and no cases of inversion of the aggressive and submissive types of behavior on the opposite one were observed. Every day during the agonistic interactions tests the winners demonstrated strong aggression―de- scribed as those groups of chronically victorious mice that demonstrated daily aggression on the third (Win3), tenth (Win10) and twenty first (Win21) days of agonistic interactions―were studied.
One-way ANOVA revealed the influence of the factor “experience” (days) on the number (F (2, 39) = 7.72; p < 0.001) and total time (F (2, 39) = 7.78; p < 0.001) of attacks, the total time of digging (F (2, 39) = 3.59; p < 0.037) and hostile behavior (F (2, 39) = 3.73; p < 0.033), the number of jumps (F (2, 39) = 4.80; p < 0.014) and rotations (F (2, 39) = 4.92; p < 0.012), and the sum of jumps and rotations (F (2, 39) = 5.21; p < 0.010). The post hoc Bonferroni test revealed significant differences (
In Win21, correlational analysis showed that the total time of attacks correlated positively with the total time of hostile behavior (R = 0.687; p < 0.007) and negatively with the sum of jumps and rotation (R = −0.566; p < 0.035) and latency of first attacks (R = −0.786; p < 0.001); additionally, the total time of hostile behavior correlated negatively with the latency of first attacks (R = −0.626; p < 0.002). As
Behavioral parameters | Win3 | Win10 | Win21 | ||||||
---|---|---|---|---|---|---|---|---|---|
Latency of first attack, sec. | 35.1 | ± | 9.0 | 87.0 | ± | 29.3 | 134.4 | ± | 54.6 |
Attacks, N | 26.1 | ± | 2.9 | 13.5 | ± | 2.6** | 13.3 | ± | 2.4** |
Attacks, sec. | 100.9 | ± | 9.3 | 60.1 | ± | 9.3** | 53.1 | ± | 9.2** |
Diggings, N | 7.1 | ± | 1.1 | 8.8 | ± | 1.9 | 13.9 | ± | 2.9 |
Diggings, sec. | 6.0 | ± | 1.0 | 10.2 | ± | 3.1 | 21.0 | ± | 6.3* |
Hostile behaviors, sec. | 115.1 | ± | 11.9 | 80.9 | ± | 9.7* | 80.1 | ± | 9.2* |
Jumps, N | 1.6 | ± | 0.9 | 3.1 | ± | 1.0 | 10.6 | ± | 3.6*+ |
Rotations, N | 1.1 | ± | 0.3 | 1.4 | ± | 0.4 | 6.3 | ± | 2.2*+ |
Jumps + rotations, N | 2.8 | ± | 1.1 | 4.5 | ± | 1.4 | 16.9 | ± | 5.6*+ |
Self-grooming, sec. | 18.9 | ± | 5.2 | 12.5 | ± | 2.9 | 10.6 | ± | 4.7 |
Aggressive grooming, % | 1/14 | 4/14 | 3/14 | ||||||
Threats, % males | 10/14 | 7/14 | 9/14 | ||||||
N animals | 14 | 14 | 14 |
Note: *p < 0.05; **p < 0.01 vs. Win3; +p < 0.05 vs. Win10.
tacks). Total time of digging behavior progressively increased from test to test of agonistic interactions.
A loser behavior became obvious after 3 days of agonistic interactions. Losers―described as those groups of chronically defeated mice on the third (Los3), tenth (Los10) and twenty first (Los21) days of agonistic interactions―were studied. In the first confrontations, the losers demonstrated mostly active defense and rearing in search for a way out of the cage. After a prolonged experience of defeats, the main behaviors were immobility and waiting. There were no differences between behaviors of Los10 and Los21.
One-way ANOVA revealed a significant influence of the factor “experience” (days) on the number (F (2, 38) = 30.60; p < 0.0001) and total time (F (2, 38) = 156.91; p < 0.0001) of active defense; on the number (F (2, 38) = 4.52; p < 0.017) and total time (F (2, 38) = 6.01; p < 0.005) of escape behavior, and on the number (F (2, 38) = 47.76; p < 0.0001) and total time (F (2, 38) = 23.89; p < 0.0001) of immobility behavior (
Behavioral parameters | Los3 | Los10 | Los21 | ||||||
---|---|---|---|---|---|---|---|---|---|
Active defense, N | 18.8 | ± | 2.3 | 5.6 | ± | 1.1*** | 2.6 | ± | 0.6 *** |
Active defense, sec. | 124.4 | ± | 8.5 | 11.5 | ± | 2.8*** | 4.9 | ± | 1.5 *** |
Escape, N | 4.7 | ± | 1.6 | 10.3 | ± | 1.8* | 11.9 | ± | 2.0 * |
Escape, sec. | 11.0 | ± | 3.1 | 20.4 | ± | 3.0 | 30.5 | ± | 5.5 ** |
Approach, N | 5.7 | ± | 1.3 | 6.8 | ± | 1.7 | 3.5 | ± | 1.0 |
Approach, sec. | 20.2 | ± | 6.3 | 28.8 | ± | 9.0 | 10.5 | ± | 3.3 |
Rearing, N | 21.3 | ± | 3.9 | 20.1 | ± | 2.4 | 22.8 | ± | 1.9 |
Rearing, sec. | 60.9 | ± | 13.3 | 45.4 | ± | 7.1 | 46.1 | ± | 4.1 |
Immobility, N | 13.6 | ± | 1.1 | 28.8 | ± | 1.8*** | 33.8 | ± | 1.6 ***+ |
Immobility, sec. | 72.2 | ± | 12.2 | 222.4 | ± | 23.4*** | 214.5 | ± | 14.1*** |
Waiting, N | 5.3 | ± | 0.8 | 7.1 | ± | 1.6 | 9.8 | ± | 1.6 |
Waiting, sec. | 40.8 | ± | 8.0 | 64.9 | ± | 19.6 | 60.8 | ± | 12.7 |
N animals | 14 | 14 | 13 |
Note: *p < 0.05; **p < 0.01, ***p < 0.01 vs. Los3; +p < 0.05 vs. Los10.
agonistic interaction was active defense in Los3 and immobility in Los10 and Los20.
One-way ANOVA did not reveal a significant influence of the factor “groups” of the winners (control, Win3, Win10, and Win21) on all parameters of partition behavior, i.e. the number of approaches and total time spent near the partition as well as number of events and the total time of rearing behavior. Among the losers, one-way ANOVA revealed a significant influence of the factor “groups” (controls, Los3, Los10, and Los21) on the total time spent near the partition (F (3, 50) = 4.61; p < 0.006) and the number (F (3, 50) = 3.56; p < 0.020) and total time (F (3, 50) = 4.67; p < 0.006) of rearing behavior.
The post hoc Bonferroni test revealed differences (
In Win10 (but not in Win3 and Win21), the correlational analysis showed that the number of attacks correlated positively with the number (R = 0.585; p < 0.028) and total time (R = 0.670; p < 0.009) spent near the partition; total time of attacks correlated positively with the total time spent near the partition (R = 0.620; p < 0.018) and latency of first attacks correlated negatively with number of approaches to the partition (R = −0.624; p < 0.017).
Behavioral observations showed that mice from all groups (controls, Win21, Los21) were calm during the test. In the first minutes of the test, the losers spent much time in the center, quickly exiting and entering the closed arms and not daring to enter the open arms. Later, the mice began to explore the open arms. Soon after the beginning of the test, the controls started exploring both the closed and open arms. All groups reached the end of the open arms, although the losers preferred to stay in the closed arms and the center of the plus-maze.
One-way ANOVA revealed a significant influence of the factor “groups” (controls, Win21, Los21) on the number of closed-arm entries (F(2, 32) = 3.85; p < 0.032), open-arm entries (F(2, 32) = 4.70; p < 0.016), number of passages (F (2, 32) = 6.12; p < 0.006) and total entries (F(2, 32) = 8.50; p < 0.001). The post hoc Bonferroni test revealed (
Behavioral parameters | Control | Win21 | Los21 | ||||||
---|---|---|---|---|---|---|---|---|---|
Closed arm entries, % | 28.9 | ± | 2.1 | 26.7 | ± | 3.4 | 37.1 | ± | 2.2+ |
Closed arm time, % | 38.5 | ± | 3.6 | 36.2 | ± | 5.1 | 42.8 | ± | 3.2 |
Central platform entries, % | 49.1 | ± | 0.5 | 49.4 | ± | 0.5 | 49.7 | ± | 0.4 |
Central platform time, % | 32.7 | ± | 1.6 | 36.9 | ± | 2.6 | 40.2 | ± | 2.5* |
Open arm entries, % | 21.9 | ± | 2.1 | 23.9 | ± | 3.2 | 13.2 | ± | 2.04*+ |
Open arm time, % | 28.8 | ± | 3.8 | 26.9 | ± | 4.0 | 16.9 | ± | 3.3* |
Total entries, N | 26.0 | ± | 1.3 | 36.5 | ± | 2.9* | 40.0 | ± | 2.6*** |
Passages, N | 2.9 | ± | 0.5 | 3.5 | ± | 1.0 | 6.8 | ± | 0.8**+ |
Peepings, N | 2.2 | ± | 0.5 | 2.4 | ± | 0.6 | 1.6 | ± | 0.5 |
Head-dips, N | 16.2 | ± | 3.5 | 25.2 | ± | 4.3 | 20.6 | ± | 4.4 |
N mice | 11 | 13 | 11 |
Note: *p < 0.05; **p < 0.01; ***p < 0.001 vs. control; +p < 0.05 vs. Win21.
number of passages was increased in Los21 compared to the controls and Win21 (p < 0.009 and p < 0.022, respectively). Additionally, the LSD test revealed differences between the controls and Los21 in open-arm entries (p < 0.028), open-arm time (p < 0.037) and central platform time (p < 0.034).
One-way ANOVA revealed a significant influence of the factor “groups” (controls, Win21, Los21) on the number of crossed squares (F (2, 37) = 13.78, p < 0.0001), number (F (2, 37) = 7.92, p< 0.001) and total time of rearing (F (2, 37) = 5.74, p < 0.007), and number of self-groomings (F (2, 37) = 3.27, p < 0.049). The post hoc Bonferroni test revealed significant differences (
Student’s t-test revealed significant differences in total time of immobility between the controls and Los21 (t = 2.26; p < 0.034); in Los21, this parameter was larger than in the controls (
On day one of this experiment, all CD-1 male mice displayed agonistic behavior in 100% of cages. Three days later, the status of the winners and losers became obvious. On the following days, there were no inversions of aggressive and submissive behaviors. Every day, the winners demonstrated aggression during all experimental periods. The scores estimating the aggressiveness in male mice―the total time and number of attacks and the total time of hostile behavior (the sum of the total time of attacks, diggings and aggressive grooming)―de- creased in the CD-1 winners. On the one hand, the decrease in aggressiveness scores could be a consequence of changes in the behavior of the losers: the active defense and escape behaviors demonstrated by the losers in the first agonistic interactions stimulated the aggression in the attacking males. Thereafter, the behavior of the losers became passive and submissive (immobility, freezing and waiting), which inhibited aggression in the winners. As a result, the active forms of offensive aggression were partly replaced by aggressive grooming and indirect aggression, i.e. digging in the losers’ territories. However, as our results show during agonistic interactions, the
structure of the aggressive behavior in the winners did not change. Most of time during the test they demonstrated attacks or diggings. On the other hand, an increased number of jumps and rotations indicated the development of a locomotor abnormality manifested in repeated stereotypic behaviors, which could affect all the other forms of behavior. The significant negative correlation found between the total time of attacks and the sum of jumps and rotations in Win21 might support this assertion. Similarly with Win21, Win10 demonstrated strong aggression, but their number of jumps and rotations did not differ from those of the controls. Repeated stereotyped behaviors, which were rarely observed in the controls, could be attributed to the development of a psychopathology in the winners [
The expressed behavioral changes were observed in the losers with a repeated experience of social defeats. In the first confrontations, the losers demonstrated an active defense behavior and, after 10 and 21 days of defeat experience, an immobility behavior. In the plus-maze immediately after the start of the test, the losers spent much time in the central platform, quickly exiting and entering the closed arms, not daring to enter the open arms. Later the mice began to explore the open arms. Pronounced anxiety was found in the losers as estimated by the increased number of closed arm entries, decreased number of open arm entries and open arm time expressed in the percentages of total test time or number of entries. The controls explored both the closed and open arms. The total time spent near the partition as a reaction to a partner in the neighboring compartment in a common cage, which might be used as a measure of the level of communication, was significantly shorter in the losers compared to the winners and controls. Thus, both tests indicated an increased anxiety-like state in the defeated mice.
Surprisingly, the CD-1 losers displayed locomotor hyperactivity (i.e. an increased number of rearings and crossed squares in the open-field test, an increased number of passages and total entries in the plus-maze test and the number of rearings in the partition test) compared to the controls. A decrease in self-grooming behavior in the losers compared to the controls in the open-field test could be attributed to an increase in escape motivated behaviors (rearing) in stressful conditions and hyperactivity. The Porsolt test revealed an increased time of immobility in the losers compared to the controls, which could indicate the development of a depression-like state. Thus, CSDS seemed to lead to the development of pronounced anxiety, depression, and locomotor and exploratory hyperactivity in the losers. The analysis of behavioral changes in mice with alternative social behaviors indicated different mechanisms of hyperactivity development, which could be accompanied by pronounced anxiety and a depression-like state in the losers and by neurological symptoms (stereotypic behavior) without anxiety in the winners.
Previously, in a similar battery of tests, we studied the effects of chronic agonistic interactions on the behavior of mice from the С57BL/6J, СВА/Lac and DBA/2 strains [
Hyperactivity scores from the open-field test, estimated as the number of crossed squares, were similar in the DBA/2 and CD-1 winners; these strains also showed significantly higher scores than those of aggressive mice from other strains. After a prolonged experience of aggression, all winners demonstrated stereotyped locomotor patterns (jumps and rotations), which were almost never demonstrated by the losers and controls. However, in the DBA/2J winners, hyperkinesia manifested most frequently in the form of sudden involuntary head movements (jerks) [
In the С57BL/6J, СВА/Lac, and CD-1 losers, a prolonged exposure to CSDS led to increased immobility behavior in all tests, a decrease in communications in the partition test, and the development of pronounced anxiety as estimated by the plus-maze scores. The development of depressiveness in the Porsolt test was observed in the С57BL/6J and CD-1 losers but not in the СВА/Lac losers. Similarly to other strains, in the DBA/2J losers, exploratory behavior and locomotor activity in the open-field test did not change under repeated defeats, and no signs of depressiveness were detected in the Porsolt test [
Hyperactivity is a symptom of bipolar disorder, which is characterized by alternate episodes of depression and mania [
We believe that outbred CD-1 mice that demonstrated, under repeated experiences of aggression and defeat, several patterns of mania and depression simultaneously may be potentially used for modeling the key symptoms of bipolar disorder. The unique behavioral changes observed in the losers and winners might also be useful for pharmacological screening of novel drugs and for the study of molecular, cellular, and genetic mechanisms of hyperactivity. Moreover, when comparing the effects of repeated social experience of aggression and defeats in mice of different strains, we can gain insights into the hereditary predisposition to behavioral disorders that occur in male mice exposed to long-term agonistic interactions.
We thank Dr. Naprimerov V.A. for help with organization of experiment. This work was supported by Russian Science Foundation, No. 14-15-00063.
Irina L.Kovalenko,Anna G.Galyamina,Dmitry A.Smagin,Natalia N.Kudryavtseva, (2015) Hyperactivity and Abnormal Exploratory Activity Developing in CD-1 Male Mice under Chronic Experience of Aggression and Social Defeats. Journal of Behavioral and Brain Science,05,478-490. doi: 10.4236/jbbs.2015.511046