Introduction: Neuroblastoma is the most common extracranial solid tumor in childhood and survival rate has improved during the last few decades. Only a few studies, related to Neuroblastoma in Saudi Arabian children, have been performed. We report epidemiologic data and our clinical experience from the department of Pediatric Hematology Oncology (PHO), King Fahad Medical City (KFMC), Riyadh, Saudi Arabia. Method: A retrospective observational study of all patients, with diagnosis of Neuroblastoma, who attended PHO-KFMC from July 2006 to June 2014 was performed. The survival periods (overall survival and disease-free survival) and the final outcomes for patients treated and followed at KFMC were recorded. The survival data were statistically correlated with the clinical, pathological and biological features of patients and tumors and compared to national and international cohorts. Results: Eight-year data were available for the 42 patients of which 22 (52.4%) were male and 20 (47.6%) were females. Age at diagnosis ranged 0 - 91 months with a mean and median of 26.3 and 18.5 months respectively. 16 (38.1%) patients were under one year and 26 (61.9%) above 1 year of age. The event-free survival (EFS) and overall survival (OS) rates were 66.5% and 71.5% respectively. EFS and OS among those who were <1 year age at presentation was 75% and 82%, whereas ≥1 yr age group had 59% and 62% survival rates respectively. Patients with tumors in the adrenal had considerably lower EFS (59%) and OS (63%); in comparison to patients with tumors sites other than the adrenal who had EFS and OS of 85% and 89% respectively. Both EFS and OS survival rates at the end of follow-up interval were 100.0%, in the low and intermediate risk groups. In contrast, patients in the high risk group had EFS and OS rates of 44% and 48% respectively. This difference was statistically significant (p < 0.05). Conclusion: Our results are very encouraging and comparable with known published international cohorts, and reveal an excellent outcome for stage 1, 2, 3 & 4 s. The prognosis for advanced (stage 4) disease remains rather poor. A collaborative Saudi-wide effort, with an emphasis on research in detecting clinical and biologic characteristics of aggressive disease and tailoring therapy, is needed.
Neuroblastoma (NBL) is the most common malignant extracranial solid tumor in the pediatric population, accounting for approximately 8% - 10% of all childhood malignancies and about 8.7 million cases per year. It primarily affects children younger than 10 years of age. Nearly 50% of patients are below the age of 2 years. NBL is slightly more prevalent in boys than in girls (1.2:1 ratio). The majority of patients (almost 70% of cases), have an abdominal primary, (45% in adrenal medulla and 25% in sympathetic ganglia) [
The 5-year event free survival (EFS) for high-risk Neuroblastoma remains rather disappointing, with less than 50% of children with stage 4 NBL being alive after 5 years, despite intensive multimodality therapy. The best outcome recently reported for high-risk NBL was achieved with intensive combination induction chemotherapy and surgery, followed by myeloablative therapy, hematopoietic stem cell rescue, and then differentiation therapy [
They are very few studies on Neuroblastoma that were undertaken in Saudi Arabia. A systematic search of the MEDLINE database from 1994 to 2014, and the Cochrane Library limited to English language articles using the terms “Neuroblastoma and Saudi Arabia” was conducted to identify all publications on Neuroblastoma in Saudi Arabian Children. 20 potentially relevant papers were identified. Of these only three papers relevant to this study were selected and reviewed. One paper, published in 1998 was a retrospective, single center study of the outcome of 20 patients in the Eastern region [
Aims: The primary aim was to assess survival trends (overall survival and disease-free survival) and final outcomes for Neuroblastoma patients treated at KFMC, correlate the survival data with the clinical, pathological and biological features of the tumor and compare findings to national and international cohorts. A secondary aim was to set up a local database for our growing number of patients with NBL.
Study design: A retrospective observational cohort study. Electronic record review of 42 patients diagnosed with Neuroblastoma and ganglioneuroblastoma who attended KFMC period July 2006 to June 2014 was carried out. Two cases of ganglioneuroma were also included. After Institutional Review Board (IRB) approval was obtained, patients data was extracted from the hospital computer system (CORTTEX, CENTRICITY & HIM), which allowed extraction of medical, radiological and laboratory data and pharmaceutical prescriptions. Data was then recorded on a comprehensive excel sheet.
Patients: A diagnosis of Neuroblastoma was made based on the histopathology obtained from the primary tumor or metastatic sites, such as bone marrow specimen. International Neuroblastoma Pathology Classification (INPC) and disease staging, in accordance with the International Neuroblastoma Staging System (INSS), was performed based on the clinicopathologic features. The size and location of the primary tumors were defined by imaging studies using computed tomography (CT) or magnetic resonance (MR) imaging. Metastatic disease was further investigated by bone marrow aspiration and biopsies from at least two sites in the iliac crests, as well as additional imaging with 131I metaiodobenzylguanidine (MIBG) and 99mTechnetium bone imaging. Positive disease findings were monitored serially by further imaging studies for treatment response. Patients were treated according to their assigned risk group (low, intermediate and high risk) and per the Children’s Oncology Group (COG)-based protocols. Complete remission was defined as the absence of any evidence of disease on any imaging modality (CT, MR imaging, MIBG or bone scintiscan) and bone marrow studies.
Other clinical date: Degree of surgical intervention, serum levels of lactate dehydrogenase and catecholamine levels in the urine were recorded. Cytogenetic studies of the MYCN gene amplification status were performed by in situ hybridization in 39 of the 42 patients. Tests for 1p deletion, 17q gain and DNA ploidy were not available at our institute during the study duration and were not performed.
Statistical Analysis: All normally distributed data was presented as mean ± SEM (standard error of the mean). Independent samples t-test was carried out to compare values between two groups. A two-sided α level of 0.05 was used for assessing statistical significance. Statistical analysis was performed using SPSS for Windows, and association between variables was determined by the Pearson correlation coefficient. Descriptive analysis was used to summarize patient characteristics. Non-parametric variables were described by medians and ranges while categorical variables were expressed by frequency. Chi-square and/or Fisher Exact tests were carried out for comparison of values between two categorical variables.
Eight-year data was available for the 42 patients with Neuroblastoma, of which 22 (52.5%) were male and 20 (47.6%) were female. Age at diagnosis ranged from 0 to 91 months, with mean and median of 26.3 ± 3.7 and 18.5 months respectively. Moreover, 16 (38.1%) patients were under one year and 26 (61.9%) above 1 year of age (
INSS risk assessment was low in 11 (26.2%), intermediate in 11 (26.2%) and high in 20 (47.6%) patients. Follow-up was terminated at 96 months during which 11 (100%) low risk, 11 (100%) intermediate risk and 12 (60.0%) high risk patients had survived. Overall survival time was 30.9 ± 4.5 (1, 96) months during which 34 (81.0%) patients survived by the terminating point and the disease free survival time was 25.4 ± 4.3 (0, 92) months.
Tumors among male patients were located in the adrenal and retroperitoneum in 17 (77.3%) and 5 (22.7%) respectively, of which 15 (68.2%) had INSS stage 3 and 4. Five distinct tumor sites were noted among females: Adrenal 9 (45.0%), retroperitoneal 4 (20%), paravertebral 3 (15%), mediastinum 2 (10%) and liver 2 (10%); of which 17 (85%) had INNS stage 3 and 4. Gross total resection, partial resection and biopsy were the extent of surgical intervention in 24 (57.1%), 5 (11.9%) and 12 (28.6%) patients respectively.
Schwannian stroma-poor Neuroblastoma was diagnosed in 34 (81.0%) patients, of whom 20 (90.9%) were males and 14 (70%) were females. Ganglioneuroblastoma was diagnosed in 6 (14.3%) patients, 5 (25%) of whom were females. Ganglioneuroma was diagnosed in 2 (4.8%) patients; one from each gender. 10 patients (23.8%) had MYCN amplification. 26 patients (61.9%) had a favorable histology according to the INPC. Lactate dehydrogenase (LDH) serum level had reference range value of 1287.8 ± 264.1 U/l (N 135 - 225 U/l) and ranged between 269 and 7666 U/l for all patients. Urine catecholamines were raised in 23 (54.8%) patients.
Patients were followed up for eight years. By the end of the study we had 8 patients who died (19%) and 34 patients (81%) are still alive. The overall survival (OS) and event-free survival (EFS) rates were 71.5% and 66.5% respectively. Males and females had OS of 71.0% and 75.0%, whereas the EFS was 66.0% and 67.0% respectively. There were 16 patients <1 year of age, of whom 15 survived (93%); and 26 patients were >1 year of age
Survived | Died | Overall Survival (OS) | Event Free Survival (EFS) | ||||||
---|---|---|---|---|---|---|---|---|---|
n | % | n | % | OS | HR (95% CI) | EFS | HR (95% CI) | ||
Gender | Female | 16 | 80.0 | 4 | 20.0 | 75.0 | 1.05 (0.26 to 4.25) | 67.0 | 1.02 (0.25 to 4.08) |
Male | 18 | 81.8 | 4 | 18.2 | 71.0 | 66.0 | |||
Age (yr) | <1 yr | 15 | 93.8 | 1 | 6.3 | 82.0 | 0.18 (0.02 to 1.51) | 75.0 | 0.19 (0.02 to 1.52) |
≥1 yr | 19 | 73.1 | 7 | 26.9 | 62.0 | 59.0 | |||
median (min, max) | 16.0 (0, 91) | 27.5 (8, 65) | |||||||
Tumor location | Others | 15 | 93.8 | 1 | 6.3 | 89.0 | 0.24 (0.03 to 1.95) | 85.0 | 0.21 (0.02 to 1.68) |
Adrenal | 19 | 73.1 | 7 | 26.9 | 63.0 | 55.0 | |||
Diagnosis | Ganglioneuroma | 2 | 100.0 | 0 | 0 | 100.0 | 100.0 | ||
Ganglioneuroblastoma | 4 | 66.7 | 2 | 33.3 | 48.0 | 24.0 | |||
Neuroblastoma | 28 | 82.4 | 6 | 17.6 | 74.0 | 2.64 (0.51 to 13.67) | 70.0 | 2.45 (0.47 to 12.67) | |
LDH Grade | <5*Normal | 25 | 89.3 | 3 | 10.7 | 73.0 | 0.26 (0.06 to 1.10) | 73.0 | 0.26 (0.06 to 1.10) |
>5*Normal | 8 | 61.5 | 5 | 38.5 | 53.0 | 53.0 | |||
median (min, max) | 480.0 (269, 5963) | 1405.5 (315, 7666) | |||||||
Urine Catecholeamines | Negative | 10 | 90.9 | 1 | 9.1 | 90.0 | 88.0 | ||
Positive | 19 | 82.6 | 4 | 17.4 | 60.0 | 0.16 (0.02 to 1.59) | 54.0 | 0.13 (0.01 to 1.31) | |
Not done | 5 | 62.5 | 3 | 37.5 | 48.0 | 0.46 (0.10 to 2.06) | 32.0 | 0.43 (0.10 to 1.94) | |
Stage (INSS) | Stage 4S, 1 & 2 | 10 | 100.0 | 0 | 0 | 100.0 | 0.03 (0.00 to 20.73) | 100.0 | 0.03 (0.00 to 33.18) |
Stage 3 & 4 | 24 | 75.0 | 8 | 25.0 | 64.0 | 59.0 | |||
MYCN Status | Not Amplified | 23 | 88.5 | 3 | 11.5 | 72.0 | 62.0 | ||
Amplified | 7 | 70.0 | 3 | 30.0 | 70.0 | 0.45 (0.07 to 2.69) | 66.0 | 0.46 (0.07 to 2.80) | |
Not done | 4 | 66.7 | 2 | 33.3 | 64.0 | 1.11 (0.18 to 6.77) | 61.0 | 1.17 (0.19 to 7.10) | |
Tumor Differentiation | Differentiated | 5 | 71.4 | 2 | 28.6 | 74.0 | 0.64 (0.15 to 2.69) | 74.0 | 0.68 (0.16 to 2.92) |
Undifferentiated | 9 | 90.0 | 1 | 10.0 | 71.0 | 65.0 | |||
INPC | Unfavorable Histology | 12 | 85.7 | 2 | 14.3 | 84.0 | 0.63 (0.13 to 3.15) | 81.0 | 0.68 (0.14 to 3.41) |
Favorable Histology | 20 | 76.9 | 6 | 23.1 | 61.0 | 52.0 | |||
INSS Risk Assessment | Low Risk | 11 | 100.0 | 0 | 0 | 100.0 | 100.0 | ||
Intermediate Risk | 11 | 100.0 | 0 | 0 | 100.0 | 0.01 (0.00 to 35.04) | 100.0 | 0.01 (0.00 to 66.06) | |
High Risk | 12 | 60.0 | 8 | 40.0 | 48.0 | 0.01 (0.00 to 55.69) | 44.0 | 0.01 (0.00 to 33.14) |
Low Risk | Intermediate Risk | High Risk | p value | |||||
---|---|---|---|---|---|---|---|---|
n | % | n | % | n | % | |||
Gender | Female | 1 | 5.0 | 10 | 50.0 | 9 | 45.0 | >0.05 (NS) |
Male | 7 | 31.8 | 7 | 31.8 | 8 | 36.4 | ||
Age (yr) | <1 yr | 7 | 43.8 | 9 | 56.3 | 0 | .0 | <0.05 (Sig) |
≥1 yr | 1 | 3.8 | 8 | 30.8 | 17 | 65.4 | ||
Median (min, max) | 4.0 (0, 15) | 11.0 (2, 91) | 26.0 (14, 75) | <0.05 (Sig) | ||||
Tumor location | Mediastinum | 0 | 0 | 1 | 50.0 | 1 | 50.0 | >0.05 (NS) |
Retroperitoneal | 1 | 11.1 | 5 | 55.6 | 3 | 33.3 | ||
Adrenal | 7 | 26.9 | 6 | 23.1 | 13 | 50.0 | ||
Liver | 0 | 0 | 2 | 100.0 | 0 | .0 | ||
Paravertebral | 0 | 0 | 3 | 100.0 | 0 | .0 | ||
Diagnosis | Ganglioneuroma | 0 | 0 | 2 | 100.0 | 0 | .0 | >0.05 (NS) |
Ganglioneuroblastoma | 0 | 0 | 4 | 66.7 | 2 | 33.3 | ||
Neuroblastoma | 8 | 23.5 | 11 | 32.4 | 15 | 44.1 | ||
LDH Grade | Not done | 1 | 100.0 | 0 | 0 | 0 | 0 | <0.05 (Sig) |
>1*Normal | 7 | 38.9 | 8 | 44.4 | 3 | 16.7 | ||
>2*Normal | 0 | 0 | 5 | 83.3 | 1 | 16.7 | ||
>3*Normal | 0 | 0 | 2 | 66.7 | 1 | 33.3 | ||
>4*Normal | 0 | 0 | 1 | 100.0 | 0 | 0 | ||
>5*Normal | 0 | 0 | 1 | 7.7 | 12 | 92.3 | ||
Median (min, max) | 315.0 (272, 446) | 480.0 (269, 7666) | 1452.0 (315, 5963) | <0.05 (Sig) | ||||
Urine catecholamines | Negative | 1 | 9.1 | 5 | 45.5 | 5 | 45.5 | >0.05 (NS) |
Positive | 5 | 21.7 | 10 | 43.5 | 8 | 34.8 | ||
Not done | 2 | 25.0 | 2 | 25.0 | 4 | 50.0 | ||
Extent of surgery | Biopsy | 1 | 8.3 | 7 | 58.3 | 4 | 33.3 | >0.05 (NS) |
Gross Total Resection | 7 | 29.2 | 7 | 29.2 | 10 | 41.7 | ||
Partial Resection | 0 | 0 | 3 | 60.0 | 2 | 40.0 | ||
Not done | 0 | 0 | 0 | 0 | 1 | 100.0 | ||
ASCT | Yes | 0 | 0 | 1 | 8.3 | 11 | 91.7 | <0.05 (Sig) |
No | 8 | 29.6 | 16 | 59.3 | 3 | 11.1 | ||
Not done | 0 | 0 | 0 | 0 | 3 | 100.0 | ||
Spinal Involvement | Yes | 0 | 0 | 4 | 100.0 | 0 | .0 | <0.05 (Sig) |
No | 8 | 21.1 | 13 | 34.2 | 17 | 44.7 | ||
Metaiodobenzyl-guanidine (mIBG) scan | Negative | 3 | 60.0 | 2 | 40.0 | 0 | .0 | >0.05 (NS) |
Positive | 5 | 13.9 | 14 | 38.9 | 17 | 47.2 | ||
Not done | 0 | 0 | 1 | 100.0 | 0 | 0 |
Stage (INSS) | Stage 4S | 3 | 60.0 | 2 | 40.0 | 0 | 0 | <0.05 (Sig) |
---|---|---|---|---|---|---|---|---|
Stage 1 | 4 | 100.0 | 0 | 0 | 0 | 0 | ||
Stage 2 | 1 | 100.0 | 0 | 0 | 0 | 0 | ||
Stage 3 | 0 | 0 | 12 | 92.3 | 1 | 7.7 | ||
Stage 4 | 0 | 0 | 3 | 15.8 | 16 | 84.2 | ||
MYCN gene Status | Amplified | 0 | 0 | 0 | .0 | 10 | 100.0 | <0.05 (Sig) |
Not Amplified | 7 | 26.9 | 15 | 57.7 | 4 | 15.4 | ||
Not done | 1 | 16.7 | 2 | 33.3 | 3 | 50.0 | ||
INPC | Favorable Histology | 8 | 30.8 | 14 | 53.8 | 4 | 15.4 | <0.05 (Sig) |
Unfavorable Histology | 0 | 0 | 2 | 14.3 | 12 | 85.7 | ||
Unknown | 0 | 0 | 1 | 50.0 | 1 | 50.0 | ||
Outcome | Survived | 8 | 23.5 | 15 | 44.1 | 11 | 32.4 | >0.05 (NS) |
Died | 0 | 0 | 2 | 25.0 | 6 | 75.0 | ||
Overall Survival (month) | Median (min, max) | 29.5 (8, 96) | 15.0 (5, 86) | 14.0 (1, 93) | >0.05 (NS) | |||
Disease Free Survival (month) | Median (min, max) | 28.0 (5, 92) | 12.0 (0, 86) | 12.0 (1, 87) | >0.05 (NS) |
NS: Not significant; Sig: Significant; ASCT: Autologous Stem Cell Transplantation.
of, whom 19 patients survived (73%) and 7 died (26%). The OS and EFS among <1 year age at presentation was 82% and 75%, whereas the ≥1 yr age group had 62% and 59% survival rates respectively.
There were 26 patients (62%) with adrenal primary, of whom 7 patients (26.9%) died, in contrast to the non- adrenal primary tumors (16 patients), of whom only one patient died (6%). Adrenal tumor patients had considerable low OS (63.0%) and EFS (59.0%) while patients with other-than-adrenal tumor sites had OS and EFS of 89% and 85% respectively. Patients with INSS stages (4S, 1 & 2) had 100.0% OS and EFS whereas those with advanced stages (stages 3 and 4) had 64% OS and 59% EFS.
LDH was elevated in all the subjects of whom 13 (31.0%) had levels five times more than the normal (LDH > 5 N). OS and EFS among LDH > 5 N patients was 60% and 53%, while other subjects had rates of 77% and 73% respectively. OS and EFS among patients with amplified MYCN tumors was 70% and 66% whereas patients with non-amplified MYCN tumors had 72% and 62%.
Disease free survival rate at the end of the follow-up interval in the low risk group was 100.0%, in the intermediate risk group was 100.0% and in the high risk group 44%. The difference was significant (p < 0.05) (
EFS among, stage 1,stage 2 and stage 4S disease subjects was 100.0% each, whereas stage 3 had 83% and stage 4 had 51% respectively (
This study has provided important data concerning the epidemiology and clinicopathologic characteristics of Saudi Arabian children with Neuroblastoma and their expected survival. Several results are in agreement with findings from other international studies. Our cohort median age was 18.5 months, similar to the study by Papaioannou and McHugh [
The EFS and OS rates of 66.5% and 71.5% respectively observed in this study is very comparable to that reported by Horner et al, where they noted the 5-year overall survival for all infants and children with Neuroblastoma has increased from 46% when diagnosed between 1974 and 1989, to 71% when diagnosed between 1999 and 2005 [
An increased level of LDH proved to be a strong prognostic indicator of adverse outcome and correlated with unfavorable histopathology in several reports [
Our result showed a lower incidence of survival among patients with adrenal primary. This is comparable a large report from the International Neuroblastoma Risk Group Project, who analyzed more than 8000 children with NBL and Ganglioneuroblastoma (GNB) and reported a higher incidence of adrenal primary (47%) and that adrenal sites were associated with inferior survival [
The prognosis of children who are older than one year with stage 4 remains poor, as seen in our study and other international studies, although it has significantly improved over the past two decades due to intensive treatment/supportive care approaches including autologous stem cell transplantation (ASCT). Our study finding of 55% OS, is slightly higher than several studies and collaborative trials. Interestingly, although the majority of stage 4S NBL do well without chemotherapy [
Our results showed a very comparable outcome to similar international cohorts, using a COG based treatment protocols, in support of the numerous publications and trials which confirmed the excellent outcome for stages 1, 2, 3 & 4 s and a dismal outcome for stage 4 in patients >1 yr old. We have set up a foundation for an excellent data base. Also the introduction of our formal comprehensive Long Term Follow up (LTFU) program will help in collecting comprehensive data regarding long term treatment effects, which will hopefully reflect on better understanding of our patients’ cohort and also identify any unique features of the Saudi Arabian patients in comparison to international cohorts.
The authors gratefully acknowledge Ms. Penelope Swift (Penny) for her review the manuscript for editing.
Zaid AlNaqib,Atif A.Ahmed,Musa AlHarbi,Fahad AlManjomi,Zaheer UllahKhan,AwatifAlanazi,OthmanMosleh,WalidBallourah,MohammedRayis,11, (2015) Neuroblastoma in Saudi Children: A Single Center Experience (2006-2014). Journal of Cancer Therapy,06,896-905. doi: 10.4236/jct.2015.610098