UV radiation and ozonation were investigated as disinfection alternatives for the wastewater treatment plant. The inactivation of total and fecal coliforms using ozone and ultraviolet radiation as separate treatments was evaluated. Different ozone concentrations (3 to 40 mg O3/L) were applied and UV fluencies ranging from 8.5 to 12 mJ/cm2 at different pH values (from 5 to 9) were tested. Best results were obtained for ozone doses near 20 mg/min with removals of 72% and 78% of fecal and total coliforms, respectively. The ozone also was capable of oxidizing organic matter in the effluent measured as COD (the highest removal obtained was 36% for 20 mg O3/min). Maximum bacterial resistance was observed at pH 7 in both cases. The UV light offered a high bacterial inactivation (over 80%) and the lowest bacterial inactivation was observed at pH 7. Finally, we obtained the electric energy per order (EEO, kWh/m3/order), defined as the electric energy (kW-h) required to degrade a contaminant by one order of magnitude in a unit volume of contaminated water, being noteworthy that EEO values for the UV process resulted were lower than those determined for the process with ozone in all the water flow tested.
Water availability is a complex issue worldwide, mainly in regions with high population density and low rainfall. It is a well-known fact that the water quality of many water supply sources is at high risk of deterioration due to discharges of poorly treated or untreated wastewater. As a result of the depletion on water quality, the incidence of waterborne illnesses has increased in the last decade, representing up to 13% of the 15 million deaths caused by infection diseases worldwide [
Municipal wastewater has been identified as one of the main sources of pathogenic agents and the potential vector of diseases as a result of accidental consumption of untreated or poorly treated wastewater, skin contact or ingestion of food species exposed to wastewater. Hence, disinfection of wastewater is mandatory as the minimum treatment before their release to natural water streams. Chlorination is one of the most widely used disinfection processes for water treatment due to its high efficiency and low cost [
Among the different alternatives to chorine disinfection, UV radiation and ozone have emerged in the last years as competitive procedures for generating safe drinking water from raw surface or underground water [
The aim of this work is to present the application of ozone and UV radiation in the disinfection and oxidation of organic matter of an effluent produced from an advanced primary treatment process and to evaluate their potential use (separately or jointly) as a polishing step for the generation of an affordable effluent for safe reuse or release into the environment. We also proposed the use of scale-up parameter and the energy required to achieve the disinfection goals of each of the processes applied to compare their performance.
Disinfection studies were run at the wastewater treatment plant (WTP) located at the Autonomous Metropolitan University—Azcapotzalco campus in Mexico City. The WTP includes a physical chemical treatment train and it was designed to process up to 1 L/s as maximum wastewater flow. The treatment train includes a coagulationflocculation step, followed by settling and filtration. In order to obtain the quality parameters of the influent a thorough sampling was performed. As required by the Mexican regulation [
Flow-chart of wastewater advanced primary treatment and Ozone-UV disinfection is presented in
UV wastewater disinfection experiments were carried out on line after the settling tank using a collimated UV radiation (254 nm wavelength) equipment (International Light Technologies, Mod. IL 1400BL) equipped with a UV radiometer (International Light Technologies, Mod. SEL 240/NS254/TD). Different contact times were tested by varying the water flow (0.16, 0.32 and 0.5 L/s) through the UV disinfection equipment; the total radiant energy was controlled by the pump rate. Two different fluencies (8.5 and 12 mJ/cm2), corresponding to 15 and 30 L/min, respectively, were tested. Different pH values (from 5 to 9), adjusted with H2SO4 (0.5 M) or NaOH (0.5 M), depending on the desired initial pH value, were also assessed to determine the influence of this parameter on the disinfection efficiency.
An ozone generator (Ozone Ecological) connected on line, coupled to a 3-HP air compressor (Craftsman), was used in the ozone disinfection experiments. Different ozone concentrations (3 to 40 mg O3/L) were tested at the same flows described for UV tests, and different pH values (from 5 to 9) were tested, adjusting with H2SO4 (0.5 M) or NaOH (0.5 M) depending on the desired initial pH value.
Samples were taken before and after every disinfection procedure in order to determine the efficiency of the tested methodology. In the case of UV-radiation disinfection, total and fecal coliforms (as CFU/100 mL) were used to monitor the process. For ozone treatment, besides the microbiological measurements, the oxidation of organic matter as COD measurements before and after the treatment was also measured. Total suspended solids (TSS), pH and temperature were determined in every experimental run before and after the treatment.
The results of the initial wastewater characterization of the composite sample obtained after the settling tank in the WTP effluent are presented in Table1 High concentration values of total and fecal coliforms and COD in the sample were observed. This implied that, despite the physical-chemical treatment originally applied to the municipal wastewater, the produced effluent was not ready to be released to the environment without posing a significant risk of contamination of surface or underground water. The observed amounts of total and fecal coliforms, 5.0 × 106 and 1.0 × 106 CFU (colony-forming units), respectively, were comparable to those previously reported for raw wastewater to which no treatment had been applied [12,13].
Besides, the value obtained for total coliforms agreed with previous reports on the concentration of these microbiological indexes in secondary effluents in other parts of Mexico [14,15]. COD concentration was slightly higher than the maximum acceptable limit in Mexican legislation for treated wastewater meant for all re-uses (150 mg/L, as stated in NOM-001-SEMARNAT-1996). TSS values (11.63 vs. 150 mg/L) and pH values were within the limits set by Mexican legislation. These results
suggested that, even though the process was efficient in removing most of the suspended solids, an important portion of the dissolved solids (as organic matter) and much of the bacteria passed through the water treatment and remained in the effluent.
The results for total and fecal coliforms (TC and FC, respectively) inactivation under different fluency and pH values are presented in
It is worthy to note that fecal coliforms were less affected by the increase in energy fluence than total coliforms. These results agreed with previous reports where 90% - 99% of E. coli inactivation was reached using 5.4 and 8.1 mJ/cm2, respectively to avoid photoreactivation [
The same trend was observed for different pH values tested (
change of pH value from 7 to 5 to maintain the homeostasis of the cell [
On the other hand, it has been reported that permeability of the outer membrane, which is the main shelter of the cell against oxidant species, increases at alkaline pH values [
Bacterial inactivation and COD removal from wastewater as function of the ozone dose (in mg/min) is presented in
Ozone was also capable of oxidizing organic matter in the effluent measured as COD. The highest removal obtained was 36% for 20 mg/min of ozone. It is well known [
Despite many disinfection processes have been developed up to full-scale commercialization, frequently this scaling-up is carried out using heuristic approaches which not always consider important parameters such as the concentration of the contaminant or treatment goals.
To provide a set of comparative scaling-up parameters for the disinfection technologies proposed in this work, a figure-of-merit proposed by the Photochemistry Commission of the International Union of Pure and Applied Chemistry (IUPAC) was used [
where: P is the rated power (kWh) of the disinfection
system, F is the water flow rate (m3/h) and ci, cf are the initial and final amount of microorganisms (CFU/mL).
Considering Equation (1), EEO values for the different experimental conditions tested in this work were determined and are shown in Table2 In all cases, fecal coliforms required the highest value of electric energy per order.
It is clear that EEO values for the UV process are lower than those determined for the ozone process in all the water flows tested. As explained earlier, EEO may represent the actual operational cost for every technology if energy cost is considered. This means that the lowest the EEO value, the lowest the operational cost will be.
For both, UV and O3 disinfection processes, the EEO value was related to the water flow. For all cases, the higher the water flow, the lower the EEO value. Thus, for UV disinfection, EEO went from 447.5 kWh/m3/order at 0.16 L/sec of water flow to 247.7 kWh/m3/order when the water flow value was increased to 0.5 L/sec. However, this difference in the EEO value is not proportional to the increase in the water flow: whereas water flow increased over 3 times, EEO was reduced only about 1.8 fold. This trend could be due to the reaction kinetics involved in the disinfection process. Usually, disinfection kinetics is proposed to be accurately described by the Chick-Watson equation. In the case of radiation driven processes, Bandala et al., (2009) [
They have found that the proposed mathematical approach fits the experimental values fairly well. In the case of the experiments described here, the presence of organic matter in the wastewater may lead to differences in the reaction kinetics which may produce non-linear relationships. The other possibility may be that the suspended solids may act as a “protective shell” for the microorganisms during the disinfection procedure and this may interfere with the proposed linear disinfection kinetics.
A similar trend was found in the case of the ozone disinfection process. In this case, the EEO value decreased about 1.5 times (from 2529.7 to 1638 kWh/m3/order)
when the water flow was changed from 0.16 to 0.5 L/sec, a lower decrease than the one found in UV disinfection. The difference may be explained by the fact that ozone is able to react with organic matter present in the wastewater as observed in
UV radiation and ozonation were investigated as disinfection alternatives for the wastewater treatment plant at the Autonomous Metropolitan University—Azcapotzalco campus, evaluating the inactivation of total and fecal coliforms.
UV light presented a high bacterial inactivation, over 80%, while fecal coliforms were less affected by the increase in energy fluency than total coliforms.
Total coliforms showed higher inactivation rates than fecal coliforms at all the pH values tested but the lowest bacterial inactivation was observed at about pH 7.
Ozone displayed a low oxidation potential, and the best results were obtained for ozone doses of about 20 mg/min with removals of 72% and 78% of fecal and total coliforms, respectively. The maximum bacterial resistance was observed at pH values near 7, in both cases.
Ozone also led to a reduction of organic matter in the effluent measured as COD, the highest removal obtained being 36% for 20 mg O3/min. Finally the EEO (kWh/m3/order) was obtained, being noteworthy that EEO values for the UV process were lower than those determined for the ozone process in all the water flows tested.
For both UV and O3 disinfection processes, the EEO value was related to water flow (in all the cases, the higher the water flow is, the lower the EEO value will be).
This work was supported by the Autonomous Metropolitan University—Azcapotzalco campus, and the National Council of Science and Technology (CONACyT) provided a scholarship to Y. Bustos.