There are two pathways for nitrate to enter into human body: drinking water and food. Therefore, nitrate contamination of drinking water and agricultural crops is of particular interest to governments and the general public owing to the possible implications for health. This work investigated nitrate contents in various crops and in different parts of each crop and in drinking water as well in the Zhangye Basin, Northwest China. It provided evidence of severe nitrate contamination of drinking water, especially, in vegetable-growing villages. As to the nitrate contamination of crops, the present study revealed that all sampled crops adjust their nitrate distributions in a way that the edible parts contain much less nitrate than non-edible parts except lettuce. The nitrate content in the edible root of lettuce reached up to 5900 mg/kg exceeding the limit set by the European Commission.
This Nitrate () is an integral part of the nitrogen cycle in the environment. It is oxidized form of nitrogen and highly soluble in water. It is invisible, odorless and tasteless. Nitrate is commonly found in soil and water and is the most effective form by which plants obtain their nitrogen. However, it is also most readily lost from soil by leaching in drainage water, which may be considered as one of the reasons for over-application of nitrogen fertilizers [1,2].
To meet the food demand of the growing world population, continued increases in food production is a necessity. Since the mid-19th century, nitrogen has been used in the agricultural system to boost productivity. It was reported that the nitrogen use increased four-fold in USA between 1960 and 1989 [
A particular concern of nitrogen use in agricultural practices is the nitrate contamination in waters and accumulation in agricultural products. The primary reason for the concern is the threat it poses to human health. The toxicity of nitrate to humans is mainly attributable to its reduction to nitrite. The major biological effect of nitrite in humans is its involvement in the oxidation of normal Hemoglobin (Hb) to met-Hemoglobin (met-Hb), which is unable to transport oxygen to the tissues. The Hb of young infants is more susceptible to met-Hb formation than that of older children and adults. Drinking water with elevated nitrate levels has been highlighted as the cause of the so-called “blue baby syndrome” [
The above-mentioned studies have greatly improved the understanding on the possible range of nitrate content in various agricultural products. However, they did not consider nitrate distribution in crops. Santamaria et al. [
The present study focused on an arid region of China where groundwater contamination by nitrate due to intensive farming has been reported. Nitrate contents in various crops and drinking water in the region were measured. The objective is to report the current state of nitrate contamination of crops and drinking water in this region.
It should be noted that the Joint Expert Committee on Food Additives and the European Commission’s Scientific Committee on Food have set an Acceptable Daily Intake (ADI) for nitrate of 3.7 mg/kg bodyweight, which is equivalent to 220 mg per day for an adult of 60 kg [19, 20]. Therefore, it may be stated that infants are more vulnerable to nitrate contamination in drinking water while adults are more affected by nitrate accumulation in foods.
In the present study, the investigations on nitrate contamination were conducted mainly in the middle reaches of the Heihe River (or the Zhangye Basin) every summer from 2009 to 2012.
The middle reaches of the Heihe River was chosen as our study area. Heihe River is the second largest inland river in China. Its main stream, with a length of 821 km, originates from the Qilian Mountains of Qinghai Province, flows through the Zhangye Basin, which is part of the ancient Silk Road, and ends up in the Inner Mongolia Autonomous Region. The catchment of the middle reaches, or the Zhangye Basin, covers an area of 1.08 × 104 km2 extending from 38˚30′ to 39˚50′N and 99˚10′ to 100˚52′E. Along the main stream, the middle reaches starts from the Yingluo Gorge and ends at the Zhengyi Gorge (
Zhangye Basin is characterized by a dry continental climate, with mean annual precipitation of about 200 mm, and annual evaporation of 2000 mm or more. The renewable water resource per capita in Zhangye is 1250 m3/yr, just 5% of the world average. Since 2001, about 50% ~ 60% of the annual total river discharge have been diverted from the middle to the lower reaches of the river following the regulation imposed by the Ministry of Water Resources of China (MWR). Consequently, groundwater abstraction and sewage water reuse for agricultural purpose have been increasing gradually to compensate for the reduced river water supply.
Grain crops, particularly seed corn, are densely cultivated in the Zhangye Basin, sustained by the continuous application of chemical nitrogen fertilizers. In 2005, the total amount of nitrogen fertilizers applied on the corn fields was more than 300 kg·ha−1·year−1 and, more recently, was more than 450 kg·ha−1·year−1 [
The study by Yang and Liu [
concentration of groundwater as high as 84.1 mg/L in the urbanized area of the Zhangye Basin and about 67% of irrigation land area was associated with medium (13 - 30 mg/L) nitrate concentration. Zhang et al. [
It should be noted here that although the WHO limit for nitrate-nitrogen in drinking water is 10 mg/L or equivalent to 45 mg/L for nitrate, Chinese standard for nitrate-nitrogen in drinking water issued in 1986 is 20 mg/L or equivalent to 90 mg/L for nitrate.
For drinking water investigation, samples were directly taken from household by home visit. The drinking water resource in the Zhangye Basin is groundwater. The supply system is the construction of community drinking well and water tower to distribute potable water through pressurized pipe network to each household within a community. The number of household surveyed was 5 in 2009, 20 in 2010, 16 in 2011 and 8 in 2012. Except 2012, home visits were randomly conducted in various villages across the basin. In 2012, drinking water testing was conducted in villages where the nitrate concentrations in crops were measured.
In the Zhangye Basin, there are three sources of irrigation water: river water; groundwater and the mixture of river water and sewage water. The use of sewage water for irrigation is because of water scarcity. In this study, three types of farmland using different source of water for irrigation were selected (
was also measured. For comparison, both mature and immature corn plants were measured. For vegetables, leaf and root were measured separately. In addition, the nitrate concentrations of irrigation water entering into the fields were measured with the Horiba compact nitrate ion meter (LAQUAtwin-B743) for water. The investigation on the build-up of nitrate in crops was conducted from August 8 to 18, 2012. All measurements were done under fine weather during daytime.
In 2009, two households out of five were found to drink water having nitrate concentrations above the WHO standard. The highest nitrate concentration found in the 2009 survey was 75 mg/l. When asking if they are concerned about nitrate contamination, one of the householders answered that there is no worry because they boil water before drinking. This highlighted the need of basic science education for farmers in this region because boiling can’t remove nitrate.
In 2010, drinking water testing found that eight households out of twenty having nitrate contents exceeding the WHO standard with the highest value of nitrate being 160 mg/l. In 2011, nine out of sixteen were above the WHO standard. The percentage of different levels of nitrate observed during the three years was shown in
In 2012, although the survey mainly focused on nitrate contamination of crops in four villages, nitrate in drinking water was also tested at the same time.
In the Gaozai village, we randomly selected two corn plants; one mature and another immature. The maturity is judged by the size and color of corn ears. The mature corn ear was 20 cm long and golden color while the immature was 10 cm long and pale green color.
decayed quickly along the stalk. In the immature plant, the nitrate concentration at the bottom was not significantly higher than the top. The maximum concentration occurred somehow in the middle of the plant where a single ear grew. The nitrate concentrations in niblets of both the mature and immature corns were 110 mg/kg and 210 mg/kg, respectively. This comparison suggests that the corn plant adjust its nitrate build-up through its growing stage in a way that the edible part contains the least nitrate. In other words, the final distribution of nitrate in corn plant is in favor of human beings. For water entering into the farmland where corn samples were taken, the concentrations of nitrate and ammonium () were found to be 20 mg/kg and 6 mg/L, respectively. The ammonium concentration was measured with the DR/890 Portable Colorimeter manufactured by the HACH Company. The data implies that high ammonium coming from waste water was converted to nitrate and then utilized by plants.
In the Genming village, which is about 10 km downstream of the Gaozai village, the concentration of nitrate in the irrigation channel was found to be as high as 110 mg/kg but 0.2 mg/L for ammonium. And the nitrate concentrations in corn plants taken from that village showed a very similar pattern as that observed in the Gaozai village. In this mixed water-irrigated area, mass production of vegetables is non-existent. According to interview with local farmers, vegetables could not be grown with the mixed water.
In the Yangjiazha village where the water from the Heihe River is used for irrigation, the nitrate concentration in the sampled corn plant is shown in
In total, 10 mature seed corns were sampled in the Zhangye Basin during the August 2012. The concentrations of root part varied from 3300 mg/kg to 3700 mg/kg with the mean and standard deviation being 3588 mg/kg and 118 mg/kg, respectively. Meanwhile, the concentrations of top part varied from 450 mg/kg to 770 mg/kg with the mean and standard deviation being 624 mg/kg and 132 mg/kg, respectively. Moreover, the concentrations of corn niblets varied from 96 mg/kg to 210 mg/kg with the mean and standard deviation being 146 mg/kg and 41 mg/kg, respectively.
In the Guojiabo and Erzha villages where the groundwater is used to irrigate vegetable farmlands, cauliflower, broccoli, lettuce, beet, tomato, Chinese cabbage, green pepper, red pepper, lantern chilli, cucumber were sampled for nitrate concentration. The measurement results are compiled in
The nitrate concentration in broccoli leaf was as high as 5300 mg/kg. However, the nitrate concentrations in broccoli thick stalk and flower head were 2600 mg/kg and 860 mg/kg, respectively. Cauliflower and Chinese cabbage contained high amount of nitrate in leaf but much less in edible part. Among all measured, lettuce was the only one having very high concentrations in both leaf and root. Beet leaf and root had quite the same but moderate amount of nitrate. It should be noted that both the leaf and root of lettuce are consumed as food by humans. For broccoli, fleshy flower head and the top part of thick stalk are edible. Beet is now usually used for making sugar.
The study by Santamaria [
The European Commission has established maximum levels (MLs) for nitrate in lettuces [
Guojiabo and Erzha villages are about 1.5 km apart and their drinking water is taken from wells about 30 m deep. Measurements found that the nitrate concentration in their drinking water was 78 mg/kg in the Guojiabo village and as high as 160 mg/kg in the Erzha village. By contrast, the nitrate concentration of the drinking water in
the Yangjiazha village was 34 mg/kg. This suggests that a cycle of groundwater contamination could have formed in vegetable-growing villages as illustrated in
Although previous studies have reported nitrate contamination of groundwater in the Zhangye Basin, it was not clear if the contaminated groundwater was actually used by local residents for drinking and cooking. By home visiting and testing nitrate concentration of water used for drinking and cooking, this study provided evidence of severe nitrate contamination of drinking water in the Zhangye Basin. Meanwhile, this work improved understanding of nitrate contamination of food by revealing that the edible parts of various crops such as corn niblets, broccoli flower head are much less contaminated compared with non-edible parts such corn stalk and Chinese cabbage leaf. The nitrate in lettuce and beet was found to have quite uniform distribution, but nitrate content in seed-corn plant can be listed in the decreasing order from brace roots, stalk to niblets. Among all measured, lettuce is the only one having very high concentration in edible parts. Therefore, it may be stated that many crops adjust their nitrate content distributions in favor of humans.
The build-up of nitrate in corn plant is affected by the type of irrigation water. The use of sewage water for irrigation due to water scarcity may cause more nitrate build-up in corn stalk. Meanwhile, production of vegetables in the Zhangye Basin mainly uses groundwater. The excessive use of fertilizer has resulted in not just higher contents of nitrate in vegetables but also groundwater contamination, which is the source of drinking water in that region.
Thanks should be given to Liu Huan, Li Ling, graduate students of Sophia University, for their assistance in field work. Appreciation also goes to Dr. T. Akiyama, the University of Tokyo, and Dr. Li Jia, the University of Niigata Prefecture, for their support and arrangement prior to and during the field investigation. Above all, the most sincere gratitude and appreciation go to Prof. Li Xin and Prof. Ma Mingguo, Cold and Arid Regions Environment and Engineering Research Institute, Chinese Academy of Sciences. Without their generous support and cooperation, this investigation would become very difficult if not impossible.