| Midpoint of pilS alignment (excluding pilS1 copy 6 and pilS6 copy2) | Tajima’s D | Significance |
| 172 | 1.8277 | Indicates balancing selection p < 0.10 |
| 178 | 1.8277 | Indicates balancing selection p < 0.10 |
| 181 - 184 | 1.9000 | Indicates balancing selection p < 0.10 |
| 213 | 1.8277 | Indicates balancing selection p < 0.10 |
| 347 - 349 | −1.7085 | Indicates purifying selection p < 0.10 |