Mutant ID

Virulent?

Overall virulence

PhoA expressed? (blue on X-phos)

PhoA present (from PCR)

Genomic DNA isolated?

68-A

no

0

yes

yes

yes

45-B

no

0

yes

yes

yes

45-A

no

0

no

4-A

no

0

yes

yes

yes

74-A3

no

0

yes

yes

yes

74-A4

no

0

yes

yes

yes

71-F11

no

0

yes

yes

yes

73-C2

no

0

yes

yes

yes

73-E1

no

0

yes

yes

yes

73-F6

no

0

yes

yes

yes

72-G2

no

0

yes

yes

yes

74-G1

no

0

yes

yes

yes

74-H2

no

0

yes

yes

yes

74-10

no

0

yes

yes

yes

73-G4

no

0

yes

yes

yes

73-G1

no

0

yes

yes

yes

71-C11

attenuated

0.5

yes

73-D1

attenuated

0.5

yes

72-A6

attenuated

0.5

no

yes

yes

74-A12

attenuated

0.5

yes

74-9

attenuated

0.5

yes

73-G6

attenuated

0.5

yes

72-G4

attenuated

0.5

yes

72-G8

attenuated

0.5

yes

74-B2

attenuated

1

yes

74-E11

attenuated

1

yes

133-A

attenuated

1

yes

74-F7

yes

2

yes

74-B6

yes

2

yes

74-C7

yes

2

yes

72-A9

yes

2

yes

73-C8

yes

2

yes

73-F9

yes

2

yes

51-A

yes

3

yes

144-A

yes

3

no

16-B

yes

4

Yes

100-A

yes

4

yes