83
L. N. ROY ET AL.
the flowing junction cell; 4) error in the experimental
emf measurement (±0.02 mV); and 5) standard potential
of the Ag-AgCl electrode (±0.03 mV). The overall un-
certainty is about ±0.009 pH unit.
5. Conclusions
All emf data are stable, reliable, and accurate. The MES
buffer solutions are considered as standards of assigned
paH and will be useful when buffer solutions of known
conventional paH are required. From Table 9, uncertainty
in pH values obtained with and without liquid junction is
±0.001 pH. Thus the operational pH values at 25˚C and
37˚C (Table 9) for one buffer solution with NaCl and
four buffer solutions matching the ionic strength of blood
serum are recommended as secondary pH standards for
the measurement of the pH of blood and other physio-
logical fluids.
6. Acknowledgements
The authors are grateful for the funding from the Na-
tional Institutes of Health (AREA), under the grant
2 R15 GM 066866-03 and the diversity supplemental
grant 3 R15 GM 066866-03 S1. The authors would also
like to thank David Jones and Morghan Olson for their
dedication and hard work. The content of this paper is
the sole responsibility of the authors and does not neces-
sarily represent the official views of the National Insti-
tutes of Health or the National Institutes of General
Medical Sciences.
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