Vol.2, No.4, 451-456 (2011)
doi:10.4236/as.2011.24058
C
opyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/AS/
Agricultural Scienc es
Sensitivity of durum wheat (Triticum turgidum) to
various postemergence herbicides
Nader Soltani*, Christy Shropshire, Peter H. Sikkema
University of Guelph Ridgetown Campus, Main Street East, Ridgetown, Ontario, Canada;
*Corresponding Aut hor: nsoltani@ridgetownc.uoguelph.ca
Received 2 September 2011; revised 15 October 2011; accepted 25 October 2011.
ABSTRACT
There are a limited number of herbicide options
available for durum wheat production in Ontario,
Canada. Four field studies were conducted in
Ontario, Canada over a three year period (2008,
2009 and 2010) to evaluate the sensitivity of
spring planted durum wheat to post-emergence
(POST) applica tions of dichlorprop/2,4 -D, dicamba/
MCPA/mecoprop, clopyralid, bromoxynil/MCPA,
pyrasulfotole/bromoxynil, thifensulfuron/tribe-
nuron + MCPA amine, fluroxypyr + MCPA ester,
tralkoxydim and fenoxaprop-p-ethyl/safener at
the manufacturers’ recommended dose and twice
that dose. Visible injury in durum wheat were 0
to 2.4% with dichlorprop/2,4-D, 0 to 6% with
dicamba/MCPA/mecoprop, 0 to 0.4% injury with
cl op yr al id , 0 to 1.4 % inj ur y wit h b ro moxynil/MCPA,
0 to 3.5% with pyrasulfotole/bromoxynil, 0 to 5%
with thifensulfuron/tribenuron + MCPA amine, 0 to
2.6% with fluroxypyr + MCPA ester, 0 to 5% with
tralkoxydim and 0.4% to 8% with fenoxaprop-p-
ethyl/safener at various evaluation dates (1, 2, 3
and 4 w eeks after treatment). Duru m wh eat height
was decreased as much as 5% with dicamba/
MCPA/mecop rop, 4 % with pyras ulfotole /bromox y-
nil and 6% with fenoxaprop-pethyl/safener but
was not affected with other herbicides evaluated.
There was no decrease in durum wheat yield
with the herbicides evaluated.
Keywords: Durum Wheat; Height; Herbicide
Sensitivity; Injury; Tolerance; Yield
1. INTRODUCTION
Durum wheat [Triticum turgidum subsp. durum (Desf.)
Husn.] is the second most important Triticum species,
next to common wheat (Triticum aestivum L.) [1]. Du-
rum wheat has yellow endosperm, high protein content
and gluten characteristics which make it popular with
processors for pasta products [1]. Globally, 30 million
MT of durum wheat are produced on approximately 18
million hectares of land [2]. Most of the durum wheat
produced in the world (nearly 8 million MT) is grown in
European Union (mainly Italy, Spain and Greece) [2].
Canada is the second largest producer of durum wheat in
the world producing nearly 4.6 million MT per year fol-
lowed by Turkey and the USA which produce 4 and 3.5
million MT, respectively [2]. Most of the durum wheat
in Canada is produced in the provinces of Saskatchewan,
Alberta and Manitoba [1]. Currently, there is limited
production of durum wheat in Ontario. Most of the
commercially grown wheat in Ontario belongs to the
species Triticum aestivum. As a result of the availability
of improved cultivars, local demand and higher returns,
there has been renewed interest in durum wheat produc-
tion in Ontario. There are a limited number of grass and
broadleaved herbicides registered for use in durum
wheat in Ontario. Durum wheat has been shown to re-
spond differently than conventional wheat to some her-
bicides [3,4]. Cultivar differences in the tolerance to
some herbicides have been reported within T. aestivum
[5-9]. More research is needed to identify herbicide op-
tions for durum wheat in Ontario.
Dichlorprop plus 2,4-D ester (premix formulated),
clopyralid and dicamba/MCPA/mecoprop are phenoxy
herbicides that are very effective for the control of a
wide spectrum of annual, biennial and perennial broad-
leaf weeds including Polygonum lapathifolium (smart-
weed), Polygonum convovulus (wild buckwheat), Cap-
sella bursa-pastoris (shepherd’s purse), Thlaspi arvense
(stinkweed), Lactuca scariola (prickly lettuce) and Tara
xacum officinale (dandelion) [10,11]. These are growth
regulating herbicides affecting respiration, translocation
and cell division [5].
Bromoxynil plus MCPA (premix formulated) is a
benzonitrile plus phenoxy herbicide that inhibits photo-
synthesis and causes uncontroll ed cell divi sion and growt h
in sensitive weeds [11]. Bromoxynil plus MCPA can
N. Soltani et al. / Agricultural Sciences 2 (2011) 451-456
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452
provide control of annual broadleaved weeds such as
Xanthium strumarium (cocklebur), Polygonum persi-
caria (ladysthumb), Chenopod ium album (common lam-
bsquarters), Sinapis arvensis (wild mustard), Solanum
Spp. (nightshades), Amaranthus retroflexus (redroot pig-
weed), Ambrosia artemisiifolia (common ragweed), Cap-
sella bursa-pastoris, Thlaspi arvense, Abutilon theo-
phrasti (velvetleaf) and suppression of perennial broad-
leaf weeds such as Convolvulus arvensis (field bind-
weed), Sonchus arvensis (perenn ial sow-thistle) and Cir-
sium arvense (Canada thistle) [10].
Thifensulfuron-methyl plus tribenuron-methyl (pre-
mix formulated) are sulfonylurea herbicides that inhibit
the activity of accetolactate synthase (ALS), an impor-
tant enzyme necessary for the biosynthesis of branched
chain amino acids, isoleucine, leucine and valine [11].
Thifensulfuron-methyl plus tribenuron-methyl is applied
at a low dose, has low mammalian toxicity and controls
several broadleaved weeds including triazine-resistant
biotypes that occur in Ontario such as Polygonum con-
vovulus, Spergula arvensis (corn spurry), Galeopsis tet-
rahit (hempnettle), Polygonum persicaria, Chenopodium
album, Sinapis arvensis, Amaranthus retroflexus, Cap-
sella bursa-pastor is, and Thlaspi arvense, [10].
Tralkoxydim and fenoxaprop-p-ethyl are post-emer-
gence (POST) herbicides that inhibit acetyl-CoA car-
boxylase (ACCase), the enzyme needed for fatty acid
synthesis and subsequent production of phospholipids
needed for cell membranes in plants. These herbicides
are very effective for the control of a wide spectrum of
annual grass species including Setaria viridis (green fox-
tail), Setaria glauca (yellow foxtail), Echinochloa crus-
galli (barnyardgrass) and Avena fatua (wild oats) [10,
11].
There is limited information publish ed on the sensitiv -
ity of durum wheat to dichlorprop/2,4-D, dicamba/MCPA/
mecoprop, clopyralid, b romoxynil/MCPA, pyrasulfotole/
bromoxynil, thifensulfuron/tribenuron + MCPA amine,
fluroxypyr + MCPA ester, tralkoxydim and fenoxaprop-
p-ethyl/safener under Ontario environmental conditions.
Currently, dichlorprop/2,4-D, clopyralid, pyrasulfotole/
bromoxynil, fluroxypyr plus MCPA ester, fenoxaprop-
p-ethyl/safener are not registered for use in durum wheat
in eastern Canada. If tolerance is adequate, the availability
of these herbicides would provide growers with additional
herbicide mode-of-actions for the control of broadleaved
and grass weeds in spring planted durum wheat.
The objective of this research was to evaluate toler-
ance of durum wheat to POST applications of dichlor-
prop/2,4-D, dicamba/MCPA/mecoprop, clopyralid, bro-
moxynil/MCPA, pyrasulfotole/bromoxynil, thifensulfu-
ron/tribenuron + MCPA amine, fluroxypyr + MCPA ester,
tralkoxydim and fenoxaprop-p-ethyl/safener at the manu-
facturers’ recommended dose and sequentially at that
dose to simulate a spray overlap in the field.
2. MATERIALS AND METHODS
Four field studies were conducted at the Huron Re-
search Station, Exeter, Ontario over a three-year period
(2008, 2009, 2010 a, 2010b). The soils for the study sites
were a Brookston clay loam with 44% sand, 33% silt,
23% clay, 4.1% OM and pH of 7.9 in 2008, 34% sand,
36% silt, 30% clay, 3.6% OM and pH of 8.0 in 2009,
and 35% sand, 43% silt, 22% clay, 4% OM, and pH of
7.8 in 2010. Seedbed prep aration con sisted of moldbo ard
plowing in the autumn followed by two passes with a
cultivator with rolling basket harrows in the spring.
Experiments were arranged in a randomized complete
block design with four replications. There were 21
treatments as listed in Table 1. Plots were 2 m wide by
10 m long. Durum wheat “Hallmark” was seeded with a
double disc drill at 150 kg·ha–1 in rows spaced 17.5 cm
apart at a depth of 4 cm on April 18, 2008, April 15,
2009 and April 3 and 5, 2010 at site a and b, respec-
tively.
Herbicides were applied POST at Zadoks stage 22 to
32. Treatments were applied with a CO2 pressurized
backpack sprayer calibrated to deliver 200 L·ha–1 aque-
ous solution at 241 kPa. The boom was 1.5 m long with
four Hypro ULD120-02 nozzle tips (Hypro, New Brigh-
ton, MN, USA) spaced 50 cm apart. All plots including
the untreated control were kept weed-free as needed.
Visible crop injury was rated on a scale of 0 to 100%
(0 = no visible injury and 100 = plant death) at approxi-
mately 1, 2, 3, 4 and 8 weeks after POST treatment
(WAT). Ten plants were randomly selected per plot and
the height from the soil surface to the highest growing
point of each plant was measured at 8 WAT. Durum
wheat was harvested at maturity (late July to early Au-
gust) using a plot combine and yields were adjusted to
14.5% moisture.
All data were subjected to analysis of variance. Tests
were combined over environments and analyzed using
the MIXED procedure of SAS [12]. Variances were par-
titioned into the random effects of location, years, loca-
tion by years, blocks within years by location and the
interactions with fixed effects (herbicide treatments).
Significance of random effects were tested using a Z-test
of the variance estimate and fixed effects were tested
using F-tests. Error assumptions of the variance analyses
(random, homogeneous, normal distribution of error)
were confirmed using residual plots and the Shapiro-
Wilk normality test. To meet assumptions of normality,
injury data were square-root or arcsine square-root trans-
N. Soltani et al. / Agricultural Sciences 2 (2011) 451-456
Copyright © 2011 SciRes. http://www.scirp.org/journal/AS/Openly accessible at
453453
formed as needed. Means were converted back to the
original scale for presentation of results. Untreated check
was not included in analysis of injury. However, all val-
ues were compared independently to zero to evaluate
treatment differences with the untreated check. Means
were separated using Fisher’s protected LSD at P < 0.05.
3. RESULTS AND DIS USSION
Environment by treatment interaction was significant
for all injury ratings. Height and yield could be com-
bined across environments. Visible injury was zero for
all treatments evaluated at 8 WAT and were not ana-
lyzed.
3.1. Injury
Injury symptoms included chlorosis, necrosis (leaf tip
burn), growth distortion and plant height reduction.
Visible injury in durum wheat was 0 to 2.4% with di-
chlorprop/2,4-D, 0 to 6% with dicamba/MCPA/me-
coprop, 0 to 0.4% injury with clopyralid, 0 to 1.4% in-
jury with bromoxynil/MCPA, 0 to 3.5% with pyrasul-
fotole/bromoxynil, 0 to 5% with thifensulfuron/ tribe-
nuron + MCPA amine, 0 to 2.6% with fluroxypyr +
MCPA ester, 0 to 5% with tralkoxydim and 0.4% to 8%
with fenoxaprop-p-ethyl/safener at the various evalua-
tion dates (Tables 1 and 2). Generally, injury was greater
at the higher dose for the herbicides evaluated although
results were not always statistically significant. Injury
Table 1. Durum wheat injury 1 and 2 WAT for various postemergence treatments. Means followed by the same letter within a column
are not significantly different according to Fisher’s Protected LSD at P < 0.05a.
Injury 1 WAT Injury 2 WAT
2008 2009 2010 2009 2010
Treatments Dose
g·ai·ha–1
%
Untreated control 0 a 0 a 0 a 0 a 0 a
Dichlorprop/2,4 -D 1017 0 a 2.0 cd 0.9 a 0 a 0 a
Dichlorprop/2,4-D 1017; 1017 0 a 2.4 cd 0.2 a 1.4 bcd 0 a
Dicamba/MCPA/ mecoprop 600 0 a 1.3 bc 0 a 0.8 abc 0 a
Dicamba/MCPA/mecoprop 600; 600 0.4 a 3.4 de 0 a 1.9 cd 0.2 b
Clopyralid 200 0 a 0 a 0 a 0 a 0 a
Clopyralid 200; 200 0 a 0.4 ab 0 a 0 a 0 a
Bromoxynil/MCPA 560 0 a 0 a 0 a 0 a 0 a
Bromoxynil/MCPA 560; 560 0 a 1.4 bc 0 a 0 a 0 a
Pyrasulfotole/bromoxynilb 213 0 a 0 a 0 a 0 a 0 a
Pyrasulfotole/bromoxynilc 213; 213 0 a 0 a 0 a 0 a 0 a
Thifensulfuron/tribenuron + MC PA amined 15 + 550 0 a 3.4 de 0 a 2.0 cd 0 a
Thifensulfuron/tribenuron + MC PA aminee 15 + 550;
15 + 550 0 a 5.0 e 0.2 a 3.9 e 0 a
Fluroxypyr + MCPA ester 108 + 562 0 a 1.4 bc 0 a 0 a 0 a
Fluroxypyr + MCPA ester 108 + 562;
108 + 562 0 a 2.6 cd 0 a 0 a 0 a
Tralkoxydimf 200 0 a 2.6 cd 0.9 a 1.4 bcd 0.6 b
Tralkoxydimg 200; 200 1.4 b 5.0 e 2.6 b 2.4 de 2.1 c
Fenoxaprop-p-eth yl/safener 92.4 4.1 c 3.4 de 4.9 c 0.4 ab 3.0 c
Fenoxaprop-p-ethyl/safener 92.4; 92.4 8.0 d 5.5 e 1.9 d 0.8 abc 5.2 d
SE 0.2 0.2 0.2 0.2 0.1
aAbbreviations: WAT, weeks after treatment. bIncluded ammonium sulfate (1.0 L·ha–1). cIncluded ammonium sulfate (1.0 L·ha–1; 1.0 L·ha–1). dIncluded
non-ionic surfactant (0.2% v/v). eIncluded non-ionic surfactant (0.2% v/v; 0.2% v/v). fIncluded mineral oil/surfactant (0.5% v/v). gIncluded mineral oil/surfac-
tant (0.5% v / v; 0.5% v/v).
N. Soltani et al. / Agricultural Sciences 2 (2011) 451-456
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454
Ta bl e 2. Durum wheat injury 3 and 4 weekd after treatment (WAT), height and yield for various postemergence treatments. Means
followed by the same letter within a column are not significantly different according to Fisher’s Protected LSD at P < 0.05.
Injury 3 WAT Injury 4 WAT
2008 &
2009 2010 2008 &
2009 2010
Treatments Dose
g·ai·ha–1
%
Heighta
cm Yiel da
t ha–1
Untreated control 0 a 0 a 0 a 0 a 71.2 abc 4.11
Dichlorprop/2,4 -D 1017 0 a 0 a 0.5 abc 0 a 71.2 abc 4.29
Dichlorprop/2,4-D 1017; 1017 1.0 ab 0 a 1.5 bcde 0 a 72.3 a 4.30
Dicamba/MCPA/mecoprop 600 1.1 ab 3.7 c 1.0 abcd 2.8 b 68.8 de 4.11
Dicamba/MCPA/mecoprop 600; 600 4.1 d 6.0 c 3.6 ef 5.1 c 67.4 ef 4.13
Clopyralid 200 0 a 0 a 0 a 0 a 72.2 ab 4.32
Clopyralid 200; 200 0 a 0 a 0.4 abc 0 a 71.6 abc 4.24
Bromoxynil/MCPA 560 0 a 0 a 0 a 0 a 7 1. 4 a bc 4. 22
Bromoxynil/MCPA 560; 560 0 a 0 a 0 a 0 a 70.7 c 4.09
Pyrasulfotole/bromoxynilb 213 0 a 1.3 b 0.2 ab 2.1 b 70.8 bc 4.15
Pyrasulfotole/bromoxynilc 213; 213 0.4 a 3.5 c 0.2 ab 3.1 b 68.4 def 3.98
Thifensulfuron/tribenuron + MC PA amined 15 + 550 0 a 0 a 0.4 abc 0 a 71.1 abc 4.21
Thifensulfuron/tribenuron + MC PA aminee 15 + 550;
15 + 550 2.1 bc 0.2 ab 2.7 def 0 a 71.3 a b c 4 .22
Fluroxypyr + MCPA ester 108 + 562 0 a 0 a 0 .2 ab 0 a 72.0 abc 4.25
Fluroxypyr + MCPA ester 108 + 562;
108 + 562 0.4 a 0 a 0.6 abc 0 a 71.2 abc 4.26
Tralkoxydimf 200 0 a 0 a 0.3 abc 0 a 72.4 a 4.19
Tralkoxydimg 200; 200 1.0 ab 0.3 ab 0.3 abc 0.5 a 72.2 abc 4.31
Fenoxaprop-p-ethyl/safener 92.4 3.2 cd 3.4 c 1.9 cde 3.0 b 69.1 d 4.14
Fenoxaprop-p-ethyl/safener 92.4; 92.4 3.9 d 5.5 c 4.7 f 4. 9 c 66.9 f 3.96
SE 0.1 0.2 0.2 0.2 0.3 0.05
aData are av eraged for 2008, 2 009, 2010. bInclud ed ammonium sulfate ( 1.0 L·ha–1). cIncl uded ammonium sulfat e (1.0 L·ha–1; 1.0 L·ha–1). dI ncluded non-io nic
surfactant (0.2% v/v). eIncluded non-ionic surfactant (0.2% v/v; 0.2% v/v). fIncluded mineral oil/surfactant (0.5% v/v). gIncluded mineral oil/surfactant (0.5%
v/v; 0.5% v/v).
with pyrasulfotole/bromoxynil increased with time.
There was no visible injury at 1 and 2 WAT with pyra-
sulfotole/bromoxynil however visible injury was as
much as 3.5% at 3 WAT and 3.1% at 4 WAT in durum
wheat. In other studies, Swan [13] found injury in winter
wheat when 2,4-D was applied prior to tillering. Sik-
kema et al. [8] found as much as 7% injury with
dicamba plus MCPA plus mecoprop and minimal injury
with 2,4-D amine, dichlorprop plus 2,4-D and bro-
moxynil plus MCPA in winter wheat. Schroeder and
Banks [7] also found that earlier applications of treat-
ments containing dicamba and dicamba plus 2,4-D, con-
tributed to wheat injury in conventional wheat. Wicks et
al. [14] and Bailey et al. [15] reported no wheat injury
with thifensulfuron-methyl plus tribenuron-methyl ap-
plied POST at 47 g·ha–1 in Virginia, USA. Hageman an d
Behrens [3] found as much as 11% injury with chlorsul-
furon another sulfonylurea herbicide in durum wheat at
Minnesota, US A.
3.2. Height
Dichlorprop/2,4-D, clopyralid, bromoxynil/MCPA, thi-
fensulfuron/tribenuron + MCPA amine, fluroxypyr +
MCPA ester and tralkoxydim applied POST at the
manufacturers’ recommended dose and twice that dose
caused no adverse effect on height of spring planted du-
rum wheat (Table 2). However, durum wheat height was
decreased as much as 5% with dicamba/MCPA/meco-
prop, 4% with pyrasulfotole/bromoxynil and 6% with
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455455
fenoxaprop-p-ethyl/safener. In other studies, Xie et al. [4]
found no adverse effect on height of durum wheat with
fenoxaprop-p-ethyl/safener applied POST at 90 g·ai·ha–1.
Sikkema et al. [8] found no adverse effect on plant
height with 2,4-D amine, bromoxynil plus MCPA, or
dichlorprop plus 2,4-D, however dicamba plus MCPA
plus mecoprop reduced height as much as 7% at 600
g·ha–1 and 11% at 1200 g·ha–1 in winter wheat. Martin et
al. [16] reported that dicamba plus 2,4-D amine and
dicamba plus MCPA reduced wheat height 11% and 10%,
respectively. In other studies, plant height reductions of
16% were seen in conventional wheat with POST herbi-
cides such as dicamba, dicamba plus MCPA plus meco-
prop, dicamba plus 2,4-D amine, dicamba plus MCPA,
saflufenacil and mesotrione [8,16-20].
3.3. Yield
There was no adverse effect on the yield of durum
wheat with the post-emergence application of dichlor-
prop/2,4-D, dicamba/MCPA/mecoprop, clopyralid, bro-
moxynil/MCPA, pyrasulfotole/bromoxynil, thifensulfu-
ron/tribenuron + MCPA amine, fluroxypyr + MCPA ester,
tralkoxydim and fenoxaprop-p-ethyl/safener at the manu-
facturers’ recommended dose or twice that dose (Table
2). This is similar to the yield response of winter wheat
with herbicides such as 2,4-D amine, bromoxynil plus
MCPA and dichlorprop plus 2,4-D [8,18,20]. However,
in other studies wheat yields were reduced as much as
39% with dicamba applied POST alone, or in combina-
tion with a phenoxy herbicide [6,16,21,22]. Derksen [23]
reported significant redu ction in wheat yield with MCPA
and 2,4-D applied at 2X doses in the autumn. Tottman
[24] also found that tank-mixes containing dicamba,
2,3,6-TBA, MCPA or mecoprop applied POST to winter
wheat can reduce grain yield. In another study, saflufenacil
applied POST reduced yield of spring wheat 13% [18].
Mesotrione applied POST caused a decrease in the yield
of spring wheat of up to 14% [20]. Hageman and
Behrens [3] found as much as 30% yield reduction with
chlorsulfuron in durum wheat under some environments
at Minnesota, USA.
4. CONCLUSIONS
Based on this study dichlorprop/2,4-D, clopyralid,
bromoxynil/MCPA, thifensulfuron/tribenuron + MCPA
amine, fluroxypyr + MCPA ester and tralkoxydim ap-
plied POST at the manufacturers’ recommended dose
can be safely used in durum wheat. However, dicamba/
MCPA/mecoprop, pyrasulfotole/bromoxynil and fenoxa-
prop-p-ethyl/safener have potential to cause injury espe-
cially at twice the manufacturers’ recommended dose in
durum wheat. Care must be taken to avoid spray over-
laps as significant crop injury can occur with twice the
manufacturers’ recommended dose in durum wheat with
these herbicides.
5. ACKNOWLEDGEMENTS
The authors acknowledge Todd Cowan for his expertise and techni-
cal assistance in these studies. Funding for this project was provided in
part by Grain Farmers of Ontario (GFO) and CanAdvance.
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