 Vol.2, No.2, 117-124 (2011) doi:10.4236/as.2011.22017 C opyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/AS/ Agricultural Sciences Long-term rice-based cropping system effects on near-surface soil compaction Jill M. Motschenbacher, Kristofor R. Brye*, Merle M. Anders Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, USA; *Corresponding Aut hor: kbrye@uark.edu Received 7 February 2011; revised 3 March 2011; accepted 30 March 2011. ABSTRACT Irrigated rice (Oryza sativa L.) production is as- sociated with frequent cycling between anaero- bic and aerobic conditions, which can lead to a greater rate of soil organic matter (SOM) decom- position, thus potentially increasing soil bulk de- nsity (BD) over time. A study was conducted in the Mississippi River Delt a region of eastern Ark- ansas, USA to evaluate the long-term effects of rice-based crop rotations, tillage [conventional tillage (CT) and no-tillage (NT)], soil fertility re- gime (optimal and sub-optimal), and soil depth (0-10 and 10-20 cm) after 10 years of consistent management on near-surface soil compaction, as measured by BD. Soil BD was greater under NT than CT in the top 10 cm, but was similar between NT and CT in the 10- to 20-cm depth interval. Soil BD differed among common rice- based cropping systems with corn, soybean, and winter wheat, but few consistent trends were evident. It appears that, even after 10 years of continuous CT or NT rice production on a silt- loam soil, substantially increased near-surface soil BD has not occurred to the point where soil compaction would be a likely culprit respon- sible for a reduced early season stand estab- lishment or crop yield differences among rice- based copping systems. Keywords: Bulk Density; Crop Rotation; Soil Organic Matter; Tillage 1. INTRODUCTION The enhancement of soil quality is vital to sustaining and improving long-term agricultural productivity, na me ly crop yields [1,2]. Soil bulk density (BD), the ratio of the dry soil mass to th e volume it occup ies, is often one of a suite of measured soil properties that is an indicator of soil quality [1,3,4 ]. Soil BD is related to soil compaction in that BD is relatively greater in compacted than in non-compacted soil. Compacted soil with a relatively large BD can negatively affect numerous soil and plant properties and processes. Soil BD has been shown to be directly related to soil streng th [5-9] and soil penetration resistance [10-14], which is another soil property that is often used to quantify soil compaction. In contrast, soil BD has been shown to be inversely related to soil or- ganic matter (SOM) [6,15], water-holding capacity [6,14, 16], soil particle size [17], total porosity [18-20], infil- tration capacity [6,21,22], hydraulic co nductivity [14,18, 20], gas exchange [23], nutrient mobility [6,24], and invertebrate movement [12,25]. Similarly, soil BD has been shown to be inversely related to seedling emer- gence [19,26] and root penetration [6,12,14,16], both of which can negatively affect yield if soil compaction is severe. Soil BD has also been shown to be affected by several crop management practices, particularly tillage and crop rotation [14,27-30]. Soil BD is generally greater under reduced tillage, specifically no-tillage (NT), due to ma- chinery traffic and the lack of surface soil disruption and mixing accomplished by annual plowing [16,18,20,29, 31,32]. Since soil BD has been shown to be inversely related to SOM [6,15], where increasing SOM generally decreases soil BD by adding additional pore space without adding much additional mass, crop rotations with a large frequency of high-residue-producing crops that are managed using cultural practices that return crop residues to the soil could consequently at least maintain a near-surface soil BD that is favorable for gas exchange, water infiltration, and plant growth. Rice (Oryza sativa L.) is one of several high-residue- producing crops, along with corn ( Zea mays L.) and win- ter wheat (Triticum aestivum L.), that is capable of pro- ducing 8.1 Mg·ha–1 of above-ground biomass under op- timal nitrogen fertilization [33,34]. Of the roughly 1.2 million ha of rice planted and 9.3 million Mg of rice grain produced in the United States annually, over 46% of the total rice area (566,800 ha) and over 45% of the
 J. M. Motschenbacher et al. / Agricultural Sciences 2 (2011) 117-124 Copyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/AS/ 118 total grain production (4.2 million Mg) occur in th e Mis- sissippi River Delta region of eastern Arkansas [35]. However, rice is unlike all other row crops in that rice is most frequently grown under flood-irrigated conditions after about one month post-emergence, where the upper- most part of the soil profile is nearly to co mpletely satu- rated [36]. To harvest rice, the flood must be released several weeks prior to the targeted harvest window in order to allow the soil to drain and dry out to achieve enough structural stability to support heavy harvesting machinery. If the soil is too wet and not sufficiently dry to provide structur al support for a large harvest combine, rice fields are often severely rutted, which can result in elevated soil BD and compaction in many areas of a field [37]. Furthermore, the decomposition of SOM is generally slower in waterlogged soil than in well-aerated soil [2, 36]. However, flood-irrigated rice fields are unique from other wetland soils in that they are often relatively dry between successive rice crops in the rotation. Aerobic soil conditions also exist during the dry periods between flooding and heavy precipitation, which stimulates the rapid breakdown of accumulated SOM. This decline in SOM can, in turn, adversely affect soil productivity, soil quality, and the overall sustainability of rice production [38]. The frequent cycling between anaerobic and aero- bic conditions can potentially lead to a greater rate of SOM decomposition [39], which could essentially in- crease the BD of the soil. Increasing soil BD over time is a reasonable concern because compacted soil may hinder short-term plant growth and long-term crop yield. Since the nature of soil physical properties are gener- ally of little concern during a rice crop-growing season due to the flooded-soil conditions, relatively few studies have examined the potential effects of rice rotations on soil physical properties, particularly soil BD. Therefore, the objective of this study was to evaluate the long-term effects of rice-based crop rotations, tillage [conventio nal tillage (CT) and no-tillage (NT)], soil fertility regime (optimal and sub-optimal), and soil depth (0-10 and 10- 20 cm) after 10 years of consistent management on near- surface soil compaction, as measured by soil BD, in the Mississippi River Delta region of eastern Arkansas. It was hypothesized that soil BD would be i) similar among soil depths under CT due to the mixing action of mechanical cultivation, but greater in the 10-20 than in the 0-10 cm depth interval under NT, ii) different among rice-based cropping systems and that the difference would be related to the frequency of rice and other high-residue-producing crops in the rotation, and iii) generally lower under optimal than sub-optimal fertiliza- tion due to greater SOM. 2. SITE DESCRIPTION This study was conducted at the University of Arkan- sas Rice Research and Extension Center (RREC) near Stuttgart, AR (34o27' N, 91o24' W), which is located in the Mississippi River Delta region of eastern Arkansas in an area known as the Grand Prairie. This study was ini- tiated in 1999 on a Dewitt silt loa m (fine, smectitic, ther- mic, Typic Albaqualf) [40], which is characteristic of Grand Prairie soils used for rice production. Prior to 1999, the study area had been fallow for sev- eral years due to a lack of irrigation capability. Vegeta- tion present consisted of a mixture of grasses and weeds that were managed by periodic mowing during the summer. In preparation for this study, the site was land-leveled to a 0.15% grade in fall 1998. Land-level- ing consisted of removing and piling the top 10 cm of soil off to the side of the area to be leveled, cutting the field to grade, and redistributing the topsoil uniformly over the field. Land-leveling is a common practice in the Mississippi River Delta region, especially in areas where rice production dominates, to facilitate uniform distribu- tion of flood-irrigation water [41]. The climate of the region is warm and wet with a 30-yr mean annual temperature minimum of 0.22˚C in January and maximum of 33.1˚C in July. The 30-yr mean annual precipitation is 131.6 cm [42]. 3. FIELD TREATMENTS AND EXPERI- MENTAL DESIGN This field study consisted of two tillage treatments [conventional tillage (CT) and no-tillage (NT)], two soil fertility treatments (optimal and sub-optimal), and 10 rice-cropping systems arranged in a randomized com- plete block with four replications (i.e., blocks) of treat- ment combinations. Each block occupied an area of 120- m long by 76-m wide (9120 m2). Soil fertility treatments were imposed as a split of each tillage treatment, while the rice rotations were horizontally stripped across the tillage-fertility combinations. Each tillage-fertility-rota- tion combination represented the experimental unit and had dimensi on s of 19 - by 6- m. The optimal soil fertility treatment followed a stan- dard fertility recommendation based on the analysis of soil samples that were collected in sp ring 1999 (Table 1). The annual soil fertility treatment consisted of P2O5 ap- plied as triple super phosphate and K2O applied as muri- ate of potash, with both fertilizers broadcast pre-plant and pre-tillage with a spreader. Nitrogen as urea was applied with a hand-spreader pre-flood at the 5-leaf stage of rice growth approximately one month after planting. Phosphorous and potassium were incorporated into the soil under CT and were left at the surface under NT.
 J. M. Motschenbacher et al. / Agricultural Sciences 2 (2011) 117-124 Copyright © 2011 SciRes. Openl y accessible at http://www.scirp.org/journal/AS/ 119 Table 1. Summary of the N, P 2O5, and K2O added to corn, soybean, rice, and wheat to comprise the sub-optimal and op- timal soil fertility treatments in a long-term rotation study at the RREC near Stuttgart, AR on a silt-loam soil. Soil Fertility (kg·ha–1) Crop Nutrient Sub-Optimal Optimal N 224 337 P2O5 67 90 Corn K2O 112 168 N 0 0 P2O5 45 67 Soybean K2O 67 135 N 112 168 P2O5 45 67 Rice K2O 67 101 N 112 168 P2O5 34 67 Wheat K2O 34 67 Following nitrogen fertilization, a 5- to 10-cm deep permanent flood was established, which was maintained annually on all of the rice plots until the rice reached physiological maturity. All other summer crops present in a given year were furrow-irrigated on an as-needed basis approximately 3 to 4 times annually, which was effectively based on the amount of rainfall received and the growth of the crop. Winter wheat was rain fed only without irrigation. Crop varieties included in the rotation treatment of this study consisted of the maj or agronomic crops grown in Arkansas. Crop rotations included: continuous rice (R), rice-soybean (RS), soybean-rice (SR), rice-corn (RC), corn-rice (CR), rice (winter wheat) [R(W)], rice (winter wheat)-soybean (winter wheat) [R(W)S(W)], soybean (winter wheat)-rice (winter wheat) [S(W)R(W)], rice- soybean-corn (RSC), and rice-corn-soybean (RCS). ‘Wells’ was the rice cultivar grown based on its local popularity among rice producers. Rice, soybean, and wheat were sown into 19-cm rows in tillage treatments using an Almaco NT drill (Almaco, Nevada, IA). The rice was drill-seeded at a rate of 100 kg seed ha–1, soy- bean at a rate of 56 kg seed ha–1, and wheat at a rate of 67 kg seed ha–1. Corn was planted in 76-cm rows at a plant populat i o n of 79,040 seeds ha–1 [43]. Rice management practices closely followed the Uni- versity of Arkansas Cooperative Extension Service rec- ommendations for stand establishment, irrigation man- agement, and pest management [44]. In CT plots, crop residues were burned and incorporated into the soil gen- erally one to two months following harvest by disking twice. Prior to planting in the spring, plots were tilled by disking once, followed by multiple passes of a light field cultivator (i.e., Triple-K) to achieve the desired seedbed for rice planting. In NT plots, crop residues were left on the surface after harvest and were not manipulated by any means prior to planting in the spring. Weed management for rice [44], soybean [45], corn [46], and wheat [47] followed recommendations made by the Arkansas Cooperative Service. Weed management for rice consisted of a pre-emergence application of 0.34 kg·ha–1 of clomazone [2-[(2-chlorobenzyl)methyl]-4, 4- dimethyl-3-isoxaolidinone] and a post-emergent applica- tion of halosulfuron [3-chloro-5-[[[[(4,6-dimethoxy-2- pyrimidinyl)amino]carbonyl]amino]sulfonyl]-1-methyl- 1H-pyrazole-4-carcoxylic acid] at 0.06 kg·ha–1 for both tillage treatmen ts. Soybean and corn were treated of 0.06 kg·ha-1 of glyphosate [N-(phosphonomethyl)glycine] in the spring and applications of paraquat [1,1’-dimethyl-4, 4’-bipyridinium ion] and flumioxazin [2-[7-fluro-hydro-3 -oxo-4-(2-propynyl)-2H-1,4-benzoxazin-6-yl]-4,5,6,7-tet- rahydro-1H-isoindole-1,3(2H)-dione] in the fall follow- ing harvest and winter-wheat planting. Corn also received a treatment of 2.3 L·ha–1 of glufosinate-ammonium [2- amino-4-(hydroxymethylphosphinyl)butanoic acid mono- ammonium salt] and 0.07 L·ha–1 of halosulfuron [3- chloro-5-[[[[(4,6-dimethoxy-2-pyrimidinyl)amino]carbo- nyl]amino]sulfonyl]-1-methyl-1H-pyrazole-4-carcoxylic- acid]. Wheat was treated with 0.35 L·ha–1 of mesosulfu- ron-methyl [methyl 2-[[[[(4,6-dimethoxy-2-pyrimidinyl) amino]car-bonyl]amino]sulfonyl]-4-[[(methylsulfonyl)a- mino]methyl] benzoate]. 4. SOIL SAMPLING At the time of soil sampling, the R an d R(W) rotations had produced a total of 10 rice crops, the RS, SR, RC, CR, R(W)S(W), and S(W)R(W) rotations had produced five rice crops with five crops in the respective rotation with corn or soybean, and the RSC and RCS rotations had produced four rice crops with three crops in the re- spective rotations with corn and soybean (Table 2). Fur- thermore, the plots that were rotated with winter wheat produced a total of 10 wheat crops. The CT treatment was imposed on all plots five months (late-October 2008) prior to soil BD sampling. Soil BD samples were col- lected in mid-March 2009 from the 0- to 10- and 10- to 20-cm depth intervals using a 4.7-cm diameter, stainless steel core chamber that was beveled to the outside to minimize compaction upon sampling. One BD sample was collected from each depth (0- to 10-cm and 10- to 20-cm) per plot at a random location between previously planted rows, for a total of 320 samples. Soil samples were oven-dried at 70˚C for 3 days and weighed to de- termine soil BD by dividing the oven-dry soil mass by the sample volume. After weighing, samples were grou nd to pass a 2-mm mesh screen to determine SOM concen- tration by wei g ht -l oss-on-ignition at 360˚C for 2 hours. Though soil BD was not measured at the onset of the study in spring 1999, land-leveling activities uniformly affected the entire study area and 10 years of consistent
 J. M. Motschenbacher et al. / Agricultural Sciences 2 (2011) 117-124 Copyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/AS/ 120 Table 2. Summary of the crop rotations and the number of rice crops and the respective rotations grown during the 10-yr study period at the RREC near Stuttgart, AR on a silt-loam soil. Crops in parentheses were grown during the winter. Number of Crops Rotation Rice Corn SoybeanWheat Continuous Rice 10 - - - Rice-Soybean 5 - 5 - Soybean-Rice 5 - 5 - Rice-Corn 5 5 - - Corn-Rice 5 5 - - Rice-(Wheat) 10 - - 10 Rice-(Wheat)-Soybean-(Wheat) 5 - 5 10 Soybean-(Wheat)-Rice-(Wheat) 5 - 5 10 Rice-Soybean-Corn 4 3 3 - Rice-Corn-Soybean 4 3 3 - management has elapsed. Therefore, it was reasonably assumed that any observed differences in soil BD among treatment combinations from the 2009 sampling repre- sented actual treatment effects rather than residual ef- fects from inherent differences among plots from the beginning of the study. 5. DATA ANALYSES The effects of tillage, fertility, crop rotation, soil dep th, replication and their interactions on soil BD and SOM were evaluated by analysis of variance (ANOVA) using the Mixed Model procedure in SAS (version 9.2, SAS Institute, Inc., Cary, NC). When appropriate, means were separated using Fisher’s protected least significant dif- ference (LSD) at the 0.05 level. In addition, linear cor- relation analyses were conducted to identify the rela- tionship between soil BD and SOM for each soil depth interval separate and combined within rotation treat- ments (version 13.31, Minitab, Inc., State College, PA). 6. RESULTS AND DIS CUS SION After 10 years of consistent rotation and fertility management and nine years of CT or NT, soil BD was affected by all treatments evaluated in this study. Statis- tical analyses showed that soil BD differed among till- age-soil depth treatment combinations (P = 0.021; Table 3) and rotation-tillage-fertility treat ment combinations (P = 0.002; Ta ble 3). There were no statistically significant block effects caused by treatment replications, so all interactions observed were exclusively a result of the imposed treatments. In the 0- to 10-cm depth interval, soil BD was 2.38% greater (P = 0.021) under NT (1.29 g/cm3) than CT (1.26 g/cm3; Figure 1). Since SOM concentration did not dif- fer between tillage treatments (Ta ble 3 ), the greater BD near the soil surface under NT can be explained by the Table 3. Analysis of variance summary of the effects of tillage, soil fertility, crop rotation, and soil depth on soil bulk density (BD) and soil organic matter content (SOM) during the 10-year rotation study at the RREC near Stuttgart, AR on a silt-loam soil. Treatment Effectz BD SOM _________ P _________ Tillage 0.229 0.110 Fertility 0.452 0.029 Tillage *Fertility 0.797 0.702 Rotation < 0.0010.333 Rotation *Tillage 0.020 0.613 Rotation *Fertility 0.995 0.792 Rotation *Tillage *Fertility 0.002 0.296 Depth 0.001 < 0.001 Depth *Tillage 0.021 0.081 Depth *Fertility 0.598 0.268 Depth *Rotation 0.160 0.050 Depth *Tillage *Fertility 0.767 0.530 Depth *Tillage *Rotation 0.301 0.215 Depth *Fertility *Rotation 0.969 0.078 Depth *Tillage *Fertility *Rotation 0.585 0.765 z Block effects were not significant in both analyses. lack of soil loosening associated with annual tillage. As would be expected, soil BD was greater in the 10- to 20- than in the 0- to 10-cm depth interval under NT (Figure 1). However, in contrast to that expected, soil BD was also greater in the 10- to 20- than in the 0- to 10-cm depth interval under CT (Figure 1). Soil BD was similar in the 10- to 20-cm depth interval between tillage treat- ments, averaging 1.41 g/cm3 across both tillage treat- ments (Figure 1). Though the 10- to 20-cm soil depth interval typically has a greater clay content than in the top 10 cm in the alluvial soils of the Mississippi River Delta region of eastern Arkansas, and since soil BD has been shown to be directly related to clay content [17], it appears that the mixing of soil due to mechanical culti- vation in CT was not substantial enough to eliminate dissimilarities among depth intervals as expected. The elevated BD in 10- to 20-cm depth interval in CT, in relation to the top 10 cm, can be partially explained by the presence of a prominent plow pan within the sam- pled depth. A prominent plow layer of approximately the 10 cm depth is common throughout much of the row- crop cultivated area in the Delta region of eastern Ar- kansas due the long h istory of annual mechanical disrup- tion by tillage [48]. The assumption of a plow pan pre- sent in CT plots is consistent with previous observations made in this same study in spring 2006, which showed that CT had significantly greater penetration resistance than to NT beginning at the 12.7-cm depth (P < 0.001) and continuing through the 25.4-cm depth (P = 0.031) [43]. A subsurface compacted layer can be created when fine soil particles dispersed during tillage settle into spaces of the soil matrix that were previously occupied
 J. M. Motschenbacher et al. / Agricultural Sciences 2 (2011) 117-124 Copyright © 2011 SciRes. http://www.scirp.org/journal/AS/ 121 Table 4. Crop rotation-soil depth treatment combination effects on soil organic matter percentage averaged across tillage and fertility treatments after 10 years of consistent management. Crops included rice (R), soybean (S), corn (C), and winter wheat (W). Soil depths included 0-cm to 10-cm and 10-cm to 20-cm. Soil Organic Matter (%)a Rotation 0-10 cm 10-20 cm R 2.085 1.428 RS 1.952 1.533 SR 1.982 1.479 RC 2.152 1.473 CR 2.237 1.499 R(W) 2.388 1.528 R(W)S(W) 2.192 1.535 S(W)R(W) 2.201 1.469 RSC 2.117 1.446 RCS 1.984 1.478 Figure 1. Tillage and soil depth effects on soil bulk density averaged across crop rotations and soil fertility levels after 10 years of consistent management. Tillage treatments included no-tillage (NT) and conventional tillage (CT). Bars with dif- ferent letters are significantly different at the 0.05 level. a.The least significan t difference at t he 0.05 l evel (LSD0.05) to co mpare among different rotations at the same depth is 0.240. All values of the same rotation with a different depth and different rotation with a dif- ferent depth are significantly different. by air, thus elevating soil BD. Furthermore, this subsur- face compacting effect of the soil can be increased by repeated machinery traffic [20] and prolonged flooded conditions for rice production, which causes the slaking of soil aggregates [49]. (1.38 g·cm–3) than NT (1.33 g·cm–3) in both soil fertility regimes (Ta ble 5), which, as mentioned previously, may presumably be caused by the settlement of fine soil par- ticles as a result of soil disruption from tilla ge combined with flooded growing conditions. This outcome suggests that greater BD associated with negative effects on plant growth and/or yield may have a greater tendency to de- velop in time under CT than under NT regardless of soil fertility regime. In addition, compared to contin uous NT (R) (1.33 g·cm–3), soil BD was 3.00% greater in the NT R(W) rotation (1.37 g·cm–3) under both soil fertility re- gimes and SOM was greater in the R(W) rotation in the 0- to 10-cm depth (2.388%) under both tillage treatments and fertility regimes than (R) (2.085%; Table 4). This result demonstrates that, despite producing a greater amount of aboveground residue in the R(W) rotation compared to continuous rice due to twice the number of high-residue-producing crops per year (Table 2), the effects of greater surface SOM decreasing soil BD are not quickly realized. However, with twice the number of crops grown per year, the R(W) rotation also experi- enced twice the number of machinery passes compared to continuous rice, so the elevated BD in the R(W) rota- tions may possibly be associated with compaction due to machinery traffic. Furthermore, penetration resistance data collected in 2006 showed that the R(W) rotation had greater resistance in the 5- to 15-cm depth (ranging from 2.5 to 4.4 MPa) than any other rotation in the study, whereas the CR rotation had the lowest resistance (ranging from 1.6 to 2.8 MPa) over the same depth Another possible contribution to greater BD in 10-to 20-cm depth in both tillage treatments was likely related to numerically lower SOM compared to the top 10 cm. Similar to previous research [6,15], the SOM concentra- tion was 43.17% greater in the 0- to 10- (2.129%) than in the 10- to 20-cm depth (1.487%) when averaged across all other treatment combinations (P < 0.001; Ta- ble 3; Table 4). Although SOM concentration did not differ significantly among tillage treatments, there was a significant interaction effect between crop rotation and sampling depth on SOM (P < 0.050; Tab le 3). Soil OM differed among crop rotations in the top 10 cm, but was similar among all crop rotations in the 10- to 20-cm depth interval. Overall, soil BD magnitudes measured in this study were well-below the typical 1.60 g/cm3 threshold at which root penetration has been reported to become limited when the soil is dry [17]. The results of this study were similar to those reported from a 3-yr rice-wheat rotation in a sandy-loam soil in Ind ia [50 ] and from a rice-rotation study in a clay-loam soil in India [51]. Averaged across soil depth, soil BD also differed (P = 0.002) among rotation-tillage-fertility treatment combi- nations (Table 3). However, few consistent trends among treatment combinations and their effect on soil BD existed. It is interesting to note that after 10 years of continuous rice, soil BD was 3.76% greater under CT Openly accessible at
 J. M. Motschenbacher et al. / Agricultural Sciences 2 (2011) 117-124 Copyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/AS/ 122 Ta b le 5 . Crop rotation-tillage-fertility treatment combination effects on soil bulk density averaged across soil depths after 10 years of consistent management. Crops included rice (R), soybean (S), corn (C), and winter wheat (W). Tillage treatments included no-tillage and conventional tillage. Soil fertility treatments included optimal and sub-optimal. Soil Bulk Density (g/cm3) a No-Tillage Conventional Tillage Rotation Optimal Sub-Optimal Optimal Sub-Optimal R 1.32 1.35 1.37 1.39 RS 1.39 1.41 1.34 1.35 SR 1.36 1.36 1.32 1.34 RC 1.38 1.35 1.35 1.36 CR 1.31 1.36 1.33 1.30 R(W) 1.35 1.39 1.37 1.34 R(W)S(W) 1.40 1.37 1.35 1.39 S(W)R(W) 1.33 1.34 1.30 1.32 RSC 1.34 1.33 1.32 1.32 RCS 1.34 1.32 1.33 1.35 aThe least si g ni ficant differen ce at t h e 0.05 lev el ( LSD0.05) to compare among same tillage, same fertility, and different rotation combinations is 0.04. The LSD0.05 to compare among same tillage, different fertility, and same rotation combinations is 0.04. The LSD0.05 to compare among different tillage, same or different fertility, and same rotation combinations is 0.03. The LSD0.05 to compare among same tillage, different fertility, and differ- ent rotation combinations is 0.07. The LSD0.05 to compare among different tillage, same or different fertility, and different rotation combinations is 0.05. interval [43]. The greater resistance in the R(W) was re- ported to be caused from a lack of a strong, deep-penetra- ting root system as is present in the CR rotation [43]. With the exception of in the CR rotation under NT, where soil BD was greater with the sub-optimal than with the optimal soil fertility regime, soil fertility regime did not affect soil BD within tillage treatments in any rotation (Table 5). Despite differences from the hy- pothesized outcome of BD in relation to fertility level, SOM concentration was 4.18% greater in optimal (1.845%) than in the sub-optimal (1.771%) fertility re- gime (P = 0.029). Soil BD within the same fertility re- gime differed between tillage treatments in all rotations except in the RC, S(W)R(W) and RCS rotations (Table 5). In contrast to that hypothesized, soil BD was unre- lated to the number of times a rice or high-residue- producing crop (i.e., rice, corn, and wheat; Table 2) was grown over the 10-yr study period in either soil depth interval separately or averaged across both soil depths. However, as might be expected, SOM concentration was highly correlated with the number of times a high- residue-producing crop (i.e., rice, corn, and wheat; Table 2) was grown over the 10-yr study period in the top 10 cm (r = 0.89, P = 0.001) an d when averaged across bo th soil depths (r = 0.90, P < 0.001). 7. AGRONOMIC IMPLICAT IONS This study demonstrated that after 10 years of consis- tent management soil BD was slightly greater under NT than CT in the top 10 cm, but soil BD was similar be- tween NT and CT in the 10- to 20-in depth interval. These results indicate that, despite BD values observed in this study being lower than the common thresho ld BD above which it is believed that root penetration is nega- tively affected, an infrequent deep-tillage operation may be needed to disrupt the developing zone of relatively compacted soil below the plow layer under CT. This study also demonstrated that soil BD differed among common rice-based cropping systems, but that differ- ences in near-surface soil BD were not clearly related to the number of high-residue-producing crops, such as rice, corn, and wheat, that were produced in a given time pe- riod. The frequent cycling between relatively dry and nearly to completely saturated soil conditions over the course of the rice growing season likely contributes to a more complex relationship between soil BD and residue returned to the soil and/or SOM accumulation. It appears that, even after 10 years of continuous CT or NT rice production on a silt-loam soil in the Mississippi River Delta region of eastern Arkansas, sub stantially increased near-surface soil BD has not developed to the point where soil compaction would be a likely culprit respon- sible for potential early season stand establishment or crop yield differences among rice-based copping sys- tems. 8. ACKNOWLEDGEMENTS This research was partially funded by the Arkansas Rice Research and Promotion Board. Field assistance provided by Terry Sells and Daniel McCarty is gratefully acknowledged. REFERENCES [1] Karlen, D.L., Andrews, S.S., Wienhold, B.J. and Zobeck, T.M. (2008) Soil quality assessment: Past, present, and future. Journal of Integrated Biosciences, 6, 3-14. [2] Pulleman, M.M., Bouma, J., van Esse n, E.A. and Meijles,
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