Vol.3, No.5, 258-262 (2011)
doi:10.4236/health.2011.35046
C
opyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/
Health
Serum 17β-estradiol and oral dryness feeling in
menopause
Farzaneh Agha-Hosseini1*, Iraj Mirzaii-Dizgah2
1Department of Oral Medicine/Dental Research Center, Dentistry School, Tehran University of Medical Sciences, Tehran, Iran;
*Corresponding Author:
2Department of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran;
Received 21 January 2011; revised 18 February 2011; accepted 27 February 2011.
ABSTRACT
The aim of this study was to compare serum
17β-estradiol of menopausal women with/without
Oral Dryness (OD) feeling, and evaluate the re-
lationship between serum 17β-estradiol and
severity of OD feeling. A case-control study was
carried out on 70 selected menopausal women
aged 40 - 77 years with or without OD feeling (35
as case, 35 as control) conducted at the Clinic
of Oral Medicine, Tehran University of Medical
Sciences. Xerostomia inventory (XI) score was
used as an index of OD feeling severity. The
serum 17β-estradiol concentration was meas-
ured by an enzyme immunoassay kit (ELISA).
Statistical analysis of Student’s t-test and
Spearman correlation coefficient was used. The
mean serum concentration of 17β-estradiol was
significantly lower in case than control. There
was a significant negative correlation between
XI score and concentration of 17β-estradiol in
menopausal women (r = –0.311, P = 0.004). It
seems that there is a negatively slight correla-
tion between OD feeling severity and serum
17β-estradiol in menopausal women.
Keywords: Menopause; Oral Dryness Feeling;
17β-Estradiol; Serum
1. INTRODUCTION
Life expectancy for women has increased significantly
during the last decade, and most women spend one third
of their lives after the menopause. Menopause is a nor-
mal developmental stage in a woman’s life, marking the
permanent cessation of menstruation [1]. It is the result
of irreversible changes in the hormonal and reproductive
functions of the ovaries [2]. It is a retrospective diagno-
sis usually made after 12 months of amenorrhea. Meno-
pause is accompanied by a number of characteristic physi-
cal changes; some of which occur in the oral cavity [3].
Oral dryness feeling or xerostomia is a subjective
sensation. It is associated with an unpleasant feeling in
the mouth and throat [4]. This complaint is more preva-
lent in menopausal women on medication, and is quite
common also in those without disease or drug usage,
unrelated to lowered salivary flow rates [5-8].
It has been suggested that changes in estrogen and
progesterone play a major role in the development of
some forms of gingival disease [9]. It seems that oral
soft tissues are sensitive to changes in female sex steroid
blood levels. The decrease in estrogen levels during
menopause is thought to affect the oral epithelial matu-
ration process, leading to thin and atrophic epithelium
[10]. It has been shown that hormone replacement ther-
apy can relieve oral discomfort in menopausal women,
further suggesting a role for female sex hormones in the
maintenance of oral tissues [11]. Our previous study has
also shown that the level of stimulated salivary 17β-
estradiol concentration is lower in menopausal women
with OD feeling, and there is a negative correlation be-
tween OD feeling severity and stimulated whole saliva
17β-estradiol [5].
After complain of caries and periodontal problems,
the most complain of people who referred to oral medi-
cine department of Tehran University of Medical Sci-
ences is dry mouth feeling. To relive their problem, con-
secutively studies have been designed. The purpose of
this study was to evaluate whether the serum 17β-estra-
diol level correlates with severity of OD feeling, and to
compare serum 17β-estradiol of menopausal women
with/without OD feeling.
2. METHODS AND MATERIALS
2.1. Subjects
The Ethics Committee of Tehran University of Medi-

F. Agha-Hosseini et al. / Health 3 (2011) 258-262
Copyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/
259259
cal Sciences (TUMS), Iran, approved the study protocol.
Informed consent was obtained from all participants.
A total of 105 menopausal women were asked to par-
ticipate in a case-control study, conducted at the Clinic
of Oral Medicine, TUMS. The participants were aged
between 40 and 77 years, had not had a menstruation
cycle for at least 24 months, and were not taking any
medication at the time of the study. Smokers, obese pa-
tients (body mass index > 30), patients with systemic
diseases (including Sjogren’s syndrome), oral candidi-
asis, or with a bad oral health condition and periodontal
disease were excluded. Of the 105 potential participants,
20 were excluded from the study based on these criteria
(13 were eliminated owing to periodontal pocket depths
more than 3 mm in multiple sites, 5 were excluded for
obesity and 2 for smoking). The remaining women were
asked to answer a questionnaire with a list of symptoms
associated with xerostomia (Table 1). Thirty five an-
swered affirmatively to at least one of the questions re-
lated to xerostomia [12,13], and formed the case group;
in fact, all the participants in the case group answered
affirmatively to at least 3 of the questions. Thirty-five
who did not answer affirmatively to any of the questions
in Ta b l e 1 formed the control group. The remaining 15
were excluded in order to match case and control groups
on the basis of age and duration of menopause. The 15
who were eliminated were done so without knowledge
of the assay data; only the demographical factors were
viewed with blinding to the assay data.
Each participant also answered another questionnaire
so that we could assess the severity of xerostomia Table
2. Xerostomia inventory (XI) score was determined as
the severity of dry mouth feeling [14]. The scores of
responses were added to provide an XI score for each
individual (the minimum possible score was 11 and the
maximum possible score was 55).
2.2. Sample Collection
Stimulated and unstimulated whole saliva were col-
lected under resting conditions in a quiet room between
9 a.m. and 12 p.m. (midday), and at least 2 hours after
the last intake of food or drink. Unstimulated salivary
samples were obtained by expectoration in the absence
of chewing movements. For pre-stimulation, the women
chewed a piece of paraffin of standard size. After 60
seconds of pre-stimulation, the participants were asked
to swallow the saliva present in the mouth. Thereafter,
whole saliva, stimulated by the same piece of paraffin,
was collected over a period of about 5 minutes. Stimu-
lated and unstimulated whole saliva were collected into a
pre-weighed and dry plastic tube. By subtracting the
empty tube weight from the saliva filled one, saliva
sample weight was determined to calculate the salivary
Table 1. Questionnaire used for selection of subjects with
xerostomia (oral dryness feeling).
Serial
Number Questions
1 Does your mouth feel dry when eating a meal?
2 Do you have difficulties swallowing any foods?
3 Do you need to sip liquids to aid in swallowing dry foods?
4 Does the amount of saliva in your mouth seem to be reduced
most of the time?
5 Does your mouth feel dry at night or on waking?
6 Does your mouth feel dry during the daytime?
7 Do you chew gum or use candy to relieve oral dryness?
8 Do you usually wake up thirsty at night?
9 Do you have problems in tasting food?
10 Does your tongue burn?
Response options: yes and no
Table 2. The xerostomia inventory (XI).
Serial
Number Questions
1 I sip liquids to help swallow food.
2 My mouth feels dry when eating a meal.
3 I get up at night to drink.
4 My mouth feels dry.
5 I have difficulty in eating dry foods.
6 I suck sweets or cough lozenges to relieve dry mouth.
7 I have difficulties swallowing certain foods.
8 The skin of my face feels dry.
9 My eyes feel dry.
10 My lips feel dry.
11 The inside of my nose feels dry.
Response options: Never (scoring 1), Hardly (2), Occasionally (3),
Fairly often (4), Very often (5)
flow rate. The flow rate was calculated in grams per
minute, which is almost equivalent to milliliters per
minute [6].
Venous blood was collected from each participant in
the morning under resting conditions in a quiet room,
between 9 a.m. and 12 p.m. The specimens were ob-
tained by venipuncture, collected in 10 ml glass vacuum
tubes without additive, and allowed to clot. The blood
was then centrifuged (2000 g, 10 min) and the serum
were separated and stored at −70˚C for later determina-
tion of 17β-estradiol concentration.

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2.3. 17β-Estradiol Concentration Assays
Serum 17β-estradiol concentration was analyzed by
ELISA technology using commercially available kits
(DRG Instruments GmbH, Germany). Wells coated with
anti-estradiol polyclonal rabbit antibody. Standards were:
0; 25; 100; 250; 500; 1000 and 2000 pg/ml. the OD was
read at 450 nm with microtiterplate reader. The range of
the assay was between 0 - 2000 pg/ml.
2.4. Statistical Analysis
For statistical analysis, the data are presented as a
mean ± SEM. The 2-tailed Student unpaired t test was
used to compare serum 17β-estradiol level between case
and control groups.
The Spearman correlation analysis was used to iden-
tify any correlation between XI score and the serum
17β-estradiol. P less than 0.05 was considered statisti-
cally significant.
3. RESULTS
The characteristics of case and control participants are
shown in Ta ble 3. There were no significant differences
between two groups in BMI, age or years after meno-
pause.
The Student’s unpaired t test showed that there was a
significant difference between the groups concerning
unstimulated whole salivary flow rate. It was lower in
the case (0.26 ± 0.12) than in the control (0.33 ± 0.01; P
= 0.001) groups. However, No significant difference was
found between the case (0.33 ± 0.02) and control (0.37 ±
0.01; P = 0.07) groups regarding stimulated saliva flow
rate.
Student t test showed that there was a significant dif-
ference in serum concentration of 17β-estradiol between
the groups (Figure 1). It was significantly lower in case
compared with the control (P = 0.01). Coefficient of
variations (CV) was 0.30 for control group and 0.41 for
case group.
Spearman correlations were performed to see if rela-
tionship existed between severity of OD feeling (XI
score) and serum 17β-estradiol. There was a negative
significant correlation between XI score and serum
17β-estradiol concentration (r = –0.311, P = 0. 004).
4. DISCUSSIONS
Oral dryness is a major complaint for many elderly
individuals, and is strongly associated with the meno-
pause [6,15,16]. The exact mechanisms that cause sensa-
tion of OD in menopausal women have not been firmly
established. However, there are reports on amelioration
of these symptoms by estrogen treatment [8]. In this
Table 3. Clinical characteristics of menopausal women with/
without oral dryness (OD) feeling.
Clinical characteristics
(mean ± S.D.)
Women without
OD feeling
Women with
OD feeling
Age (years) 56.55 ± 6.31 55.87 ± 6.55
Years-since-menopause 10.0 ± 6.9 10.1 ± 7.6
Body mass index
(BMI, Kg/m2) 24.2 ± 2.5 24.8 ± 2.1
Xerostomia inventory
(XI) score 12.21 ± 1.2 30.24 ± 6.14
Figure 1. Comparison of serum concentration of 17β-estradiol
(means ± SEM) between menopausal women with oral dryness
feeling and healthy individuals as determined by unpaired stu-
dent’s t-test, *P = 0.001.
study, the relationship between serum 17β-estradiol level
and OD in menopausal women was investigated.
Our data showed that serum 17β-estradiol concentra-
tion is significantly lower in menopausal women suffer-
ing from oral dryness. It also appears that OD severity
correlates with serum 17β-estradiol concentration. It has
been shown that the composition of saliva in menopausal
women is estrogen dependent [17]. In addition, hormo-
nal replacement therapy has been reported to reduce the
complaints of dry mouth feeling resulting in improved
oral wellbeing. On the other hand, oral discomfort is a
common symptom of menopause, it often occurs without
overt clinical signs, and it frequently resolves during
appropriate hormone replacement therapy [8,15,16,18,
19]. It seems that a positive relationship exists between
ovarian hormone modifications and changes in the oral
mucosa, and sex hormone withdrawal might be a cause
in incidence of oral dryness feeling in menopausal
women [10]. Gingival tissue is known to be sensitive to
changes in the hormonal balance, especially to changes
in female sex steroids [9]. It has been suggested that
changes in hormone levels and types (predominantly

F. Agha-Hosseini et al. / Health 3 (2011) 258-262
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261261
estrogen and progesterone) play a great role in the de-
velopment of some forms of gingival or periodontal dis-
ease [20]. Clinical reports of gingival enlargement con-
current with the onset of puberty and during pregnancy,
or gingival atrophy and surface desquamation during
menopause, have led some investigators to regard the
gingival as a secondary target organ for the direct action
of female sex hormones [9]. Also, human gingiva has
been showed to metabolize estrogens [21].
Consistent with our previous studies on stimulated
whole salivary 17β-estradiol [5], and serum and saliva
progesterone [22] the results showed that subjects with
OD had significantly lower serum 17β-estradiol concen-
tration in menopausal women with OD feeling compared
with the control group. In addition, a negative correla-
tion between serum 17β-estradiol level and severity of
OD in menopausal women was also observed. Therefore,
it is possible that there is a relationship between serum
17β-estradiol level and OD feeling in menopausal
women.
We also found that unstimulated, but not stimulated
whole saliva flow rate, was significantly lower in meno-
pausal women with OD feeling, in comparison with
women without OD feeling, which was consistent with
our previous studies [5,6,22,23]. It can be concluded that
menopausal women with OD feeling suffer from re-
duced salivary flow rate in unstimulated conditions.
However, it may alleviate in a stimulated state.
Our research had not planned for day-to-day collec-
tion of serum sample, because we anticipated and ex-
perienced resistance from the study participants espe-
cially in the control group, so we took only one sample.
There were other limitations to this study, e.g., this was a
cross-sectional study and longitudinal studies may find
similar or different results.
5. CONCLUSIONS
It seems that there is a slight negative correlation be-
tween OD feeling severity and serum 17β-estradiol in
menopausal women.
6. ACKNOWLEDGEMENTS
Research conducted with Grant from Tehran University of Medical
Sciences, Tehran, Iran. The authors declare that there is no conflict of
interests.
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