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Vol.3, No.4, 238-244 (2011) Health doi:10.4236/health.2011.34042 Copyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/ Internal environment for growth of cancer cells in mice: hypothermia, anemia and lymphocytopenia Mayumi Watanabe1, Hiroaki Matsumoto1, Chikako Tomiyama2, Kohei Akazawa3, Toru Abo 1* 1Department of Immunology, Niigata University School of Medicine, Niigata, Japan; *Corresponding author: immunol2@med.niigata-u.ac.jp 2School of Health Sciences, Faculty of Medicine, Niigata University, Niigata, Japan; 3Department of Medical Informatics, Niigata University Medical & Dental Hospital, Niigata, Japan Received 14 February 2011; revised 15 March 2011; accepted 5 April 2011. ABSTRACT The internal environment in tumor-bearing mice was investigated in det ail. When EL-4 ly mphoma cells (5 × 106 cells/mouse) were intraperitoneally inoculated into C57BL/6 mice, approximately 70% of the mice died by day 24. In this regard, the internal environment w as compared between control mice, surviving mice and tumor-bearing mice which died. The most prominent sign was hypothermia (<34˚C from day 16) seen in tu- mor-bearing mice which died. Control mice and surviving mice did not show such hypothermia (around 38˚C). Other changes, including anemia and lymphocytopenia, were also seen in tu- mor-bearing mice w hich died, but not in control mice and surviving mice. Immunological study revealed that NKT cells, which have been pre- viously identified to be the major effector lym- phocytes against EL-4 cells, almost disappeared, especially in the liver of tumor-bearing mice which died. These results suggest that a spe- cific internal environment might be required for continuous tumor growth in vivo. Such internal environmental factors include hypothermia, ane- mia and severe immunosuppression. Keywords: Cancer; Hypothermia; Anemia; Immunosuppression; Glycolysis; Mitochondria 1. INTRODUCTION Many clinicians have noticed that cancer patients have anemia and immunosuppression, especially those in their advanced stage [1-7]. One hypothesis is that such condi- tions are the results from cachexia in cancer patients [8-12]. Cachexia is known to be mediated by TNFα, TGFβ, etc., and these responses are common phenomena seen in patients with advanced cancer. In a recent study, it was demonstrated that cancer pa- tients even in their early stage suffered from hypother- mia, anemia and immunosuppression [13]. This raised the possibility that these internal conditions might be essential for the onset of cancer and the maintenance of cancer cell growth. Cancer cells carry few mitochondria in the cytoplasm and their major energy production is supported by glycolysis [14-16]. To maintain the glyco- lysis pathway, hypothermia and anemia (hypoxia) are required. In light of these findings, the object of this research is to investigate whether a similar internal environment is seen in tumor-bearing mice. Under the present applied protocol using EL-4 lymphoma cells [17,18], approxi- mately 70% of the mice inoculated with EL-4 cells died of cancer while the remaining 30% of the mice survived irrespective of the inoculation. Therefore, the data were analyzed between three groups, i.e., control mice, sur- viving mice and tumor-bearing mice which died. As a result, only tumor-bearing mice which died consistently showed hypothermia, anemia and immunosuppression. 2. MATERIALS AND METHODS 2.1. Mice C57BL/6 (B6) mice [19] were purchased from Clea Japan (Tokyo, Japan). All mice were used at 6–8 weeks of age in these experiments. These mice were fed under specific pathogen-free conditions in the animal facility of Niigata University (Niigata, Japan). 2.2. Tumor Inoculation EL-4 lymphoma cells (5 × 106 cells/mouse) were in- jected into mice intraperitoneally (i.p.) [17,18]. This cell line is of B6 background. At day 16 after tumor inocula- tion, their liver, spleen, thymus and bone marrow (BM) were harvested. M. Watanabe et al. / Health 3 (2011) 238-244 Copyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/ 239 2.3. Cell Preparation Mice anaesthetized with ether were sacrificed by ex- sanguination from the subclavian artery and vein, and various organs were removed. Hepatic lymphocytes were prepared as previously described [20]. Briefly, the liver was pressed through 200-gauge stainless steel mesh and suspended in Eagle’s MEM medium supplemented with 5 mM Hepes (Nissui Pharmaceutical, Tokyo, Japan) and 2% FCS. After one washing, the pellet was resus- pended in 35% Percoll solution containing 100 U/ml heparin and centrifuged at 2000 rpm for 15 min. The pellet was resuspended in red blood cell (RBC) lysis solution (155 mM NH4Cl, 10 mM KHCO3, 1 mM EDTA, 170 mM Tris, pH 7.3) and then washed twice with the medium. Splenocytes and thymocytes were obtained by forcing the spleen and thymus, respectively, through stainless steel mesh. BM cells were obtained by flushing femurs with medium. Splenocytes and BM cells were treated with 0.2% NaCl solution to remove RBC. 2.4. Measurement of Body Temperature (Rectal Temperature), Glucose, Lactate and β-ketone Body temperature (rectal temperature) was deter- mined by using THERMAC SENSOR (Shibaura Denki Co., Tokyo). Blood glucose and β-ketone were measured by Precision Xtra (TM) (Abbott Japan Co., Ltd., Chiba, Japan). Urine glucose level was checked by Bayer Keto- Diastix urine checker (Siemens Healthcare Diagnostics, UK). To analyze the hematological parameters, the numbers of leukocytes and erythrocytes (RBC) in the blood were determined by hemocytemeter. Granulocytes, lymphocytes and monocytes were determined by Giemsa staining. 2.5. Flow Cytometric Analysis The phenotype of cells was identified by two-color immunofluorescence tests [20]. The reagents used here included anti-CD3ε (145-2C11), anti-IL-2Rβ (TMβ-1), and anti-NK1.1 (PK136) mAbs (PharMingen, San Diego, CA). All mAbs were used in fluorescein isothiocyanate (FITC)-, phycocerythrin (PE)-forms. To prevent nonspe- cific binding of mAb, anti-CD16/CD32 (2.4 G2) mAb was added before staining with labeled mAbs. The sus- pended cells (5 × 105 – 2 × 106/tube) were stained with mAbs and stained cells were analyzed with a FACScan (Becton-Dickinson). Dead cells were excluded by for- ward scatter, side scatter, and propidium iodide gating. 2.6. StatisticalAnalysis The difference between the values was determined by ANOVA and a post-hoc test. 3. RESULTS 3.1. Cancer Death and Hypothermia Tumor-bearing mice began to die around day 20 after the inoculation of cancer cells (Figure 1(a)). Up to day 24, approximately 70% of the mice died. Since these results (i.e., 70% died and 30% survived) were consis- tently repeated, we separated the mice into two groups, i.e., those which died and those which survived. In comparison with control mice, the body weight of two tumor-bearing mice was almost comparable up to day 20 (Figure 1(b)). However, body temperatures of two groups were found to be different. From day 16, the group of mice which eventually died showed a decrease of body temperature. All mice which died suffered from hypothermia. 3.2. Glucose in the Blood and Urine of Tumor-Bearing Mice In our previous human study [13], some cancer pa- tients had hyperglycemia as well as hypothermia. We therefore examined whether a variation of blood glucose was also shown in tumor-bearing mice (Figure 2(a)). Control mice and mice which survived did not show any variation of blood glucose. In contrast, tumor-bearing mice which died finally showed a decreased level of blood glucose. This was due to the leakage of glucose into the urine (Figure 2(a), bottom). The levels of lactate and β-ketone were comparable between all tested groups (Figure 2(b)). There was a tendency that these levels were slightly high in tumor-bearing mice, especially in mice which died. 3.3. Anemia and Lymphocytopenia in Tumor-Bearing Mice The number of RBC was enumerated in the blood of control and tumor-bearing mice (Figure 3(a)) From day 16 after tumor inoculation, anemia was seen in tu- mor-bearing mice. This was true in both tumor-bearing mice which died and those which survived. The number of WBC was also enumerated. However, there was not big difference among all tested mice. We then examined the ratios of leukocyte subsets, including granulocytes, lymphocytes and monocytes (Figure 3(b)). The most prominent change was seen in tumor-bearing mice which died. Namely, the ratios of granulocytes and monocytes increased and that of lymphocytes decreased from day 16. This pattern showed severe immunosuppression at the late stage in tumor-bearing mice which died. M. Watanabe et al. / Health 3 (2011) 238-244 Copyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/ 240 (a) (b) Figure 1. Time-kinetic study after tumor inoculation. (a). Survival ratio, (b). Body weight and body temperature (rectal temperature). Ten mice were used to determine survival ratio. The mean and one SD of body weight and body temperature were produced from 4 mice at each point of time. † death ** P < 0.01. (a) (b) Figure 2 Parameters in the blood and urine. (a). Glu- cose in the blood and urine, (b). Lactate and β-ketone in the blood. The mean and one SD of parameters in the blood were produced from 4 mice at each point of time. † death ** P < 0.01. M. Watanabe et al. / Health 3 (2011) 238-244 Copyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/ 241 (a) (b) Figure 3. Number and proportion of cells in the blood. (a). The number of RBC, (b). The proportion of granu- locytes, lymphocytes and monocytes. The mean and one SD of parameters in the blood were produced from 4 mice at each point of time. † death ** P < 0.01. 3.4. Decrease in the Proportion of NKT Cells Which subsets of lymphocytes decreased in tumor- bearing mice were examined (Figure 4). Two-color im- munofluorescence tests were conducted to identify lym- phocytes subsets. Lymphocytes were isolated from the liver, spleen and thymus on day 16. Two-color staining for CD3 and IL-2Rβ identified IL-2Rβ+CD3int cells, whereas that for CD3 and NK1.1 identified NKT cells (NK1.1+CD3int). The proportions of both IL-2Rβ+CD3int cells and NKT cells were found to decrease in the liver of tumor-bearing mice which died. Surviving tumor- bearing mice did not show such a decrease. Figure 4. Immunofluorescence tests to identify lymphocyte subsets. Lymphocytes were isolated from the liver, spleen and thymus at day 16 after tumor inoculation. Two-color staining for CD3 and IL-2Rβ (or NK1.1) was conducted to identify NK cells (CD3–IL-2Rβ+ or CD3–NK1.1+), NKT cells (CD3intNK1.1+) and T cells (CD3+IL-2Rβ– or CD3+NK1.1–). Two-color staining for CD4 and CD8 was conducted to iden- tify double-positive cells (CD4+CD8+) in the thymus. Num- bers in the figure represent the percentages of fluores- cence-positive cells. The difference is indicated by arrow- heads. M. Watanabe et al. / Health 3 (2011) 238-244 Copyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/ 242 Two-color staining of thymocytes for CD4 and CD8 was also conducted (Figure 4, bottom). The proportion of double-positive CD4+CD8+ cells prominently de- creased in tumor-bearing mice which died. In other words, this pattern showed immunosuppressive states as reported previously. Thymic atrophy (50%) was also observed in these mice. Two-color staining for CD3 and B220 to identify CD3–B220+ B cells and such staining for Mac-1 and Gr-1 to identify Mac-1+Gr-1– macrophages and Mac- 1+Gr-1+ granulocytes were then conducted (Figure 5). Lymphocytes were isolated from the liver, spleen and Figure 5. Identification of B cells and macrophages. Mononuclear cells were isolated from the liver, spleen and bone marrow (BM). Two-color staining for CD3 and B220 and that for Mac-1 and Gr-1 was conducted to identify B cells (B220+CD3–) and macrophages (Mac-1+Gr-1–). Num- bers in the figure represent the percentages of fluorescence- positive cells. The difference is indicated by arrowheads. BM on day 16. The proportion of CD3–B220 + B cells increased in the liver and spleen of tumor-bearing mice which died. Surviving tumor-bearing mice did not show such change. The proportion of Mac-1+Gr-1– macrophages tended to increase in the liver of tumor-bearing mice. The pro- portion of Mac-1+Gr-1+ granulocytes tended to slightly increase in the BM of tumor-bearing mice. These changes were seen in both tumor-bearing mice which died and those which survived. 4. DISCUSSION In the present study, we demonstrated that hypother- mia appeared consistently in tumor-bearing mice and that these mice finally died of cancer. Such hypothermia was observed around day 16 after EL-4 cell inoculation and at this stage cancer cells grew well. In addition to hypothermia, anemia and immunosuppression were pre- sent in tumor-bearing mice. In our recent study in hu- mans [13], cancer patients always suffered from hypo- thermia, anemia (hypoxia) and immunosuppression. In this regard, it is speculated that a specific internal envi- ronment such as hypothermia, anemia and immunosup- pression might be required for the maintenance of cancer cell growth. Several investigators have noticed that cancer cells have few mitochondria in the cytoplasm and produce energy by the glycolysis pathway [14-16]. Recent cu- mulative evidence also supports this earlier observation [21-25]. On the other hand, normal cells, which divide less (i.e., cardiac cells and red muscle fiber cells), carry many mitochondria and produce energy mainly by the oxidative phosphorylation [13,26]. Concerning this, the specific internal environment of hypothermia, anemia and immunosuppression is essential to grow cancer cells in vivo in both animals and humans. Indeed, at the pre- sent experimental protocol, 30% of mice with EL-4 in- oculation survived and the inoculated EL-4 cells did not grow well in the peritoneal cavity. None of these mice showed hypothermia, anemia and immunosuppression. In addition to anemia (hypoxia), hypothermia is im- portant to maintain the glycolysis pathway which sup- ports cell division [13]. Even normal dividing cells such as sperms and skin cells have few mitochondria in the cytoplasm and the optimal temperature is around 32 to 33˚C (Ta b l e 1). For example, the testicles of males de- scend in the fetal stage. This is to obtain an internal en- vironment of hypothermia for sperm cell division [27,28]. It is speculated that tumor cell division may similarly or more strictly require hypothermia. In our earlier study, we reported that the major effec- tor lymphocyte subsets were invariant NKT cells in mice which were injected with EL-4 lymphoma cells [17,18]. M. Watanabe et al. / Health 3 (2011) 238-244 Copyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/ 243 Table 1. Energy production in body. Glycolysis Mitochondria Site cytoplasm mitochondria Oxygen – + Source glucose glucose and lipid Temperature 32˚C > 37˚C Usage prompt force cell division continuous force suppression of cell division ATP production quick (×100) slow (×1) Efficiency low (2 ATP/glucose) high (36 ATP/glucose) The actual cytotoxicity against EL-4 cells was mediated by such NKT cells. Mice inoculated with EL-4 cells in the present protocol also showed both lymphocytopenia in the blood and a decreased proportion of NKT cells in the liver. These immunosuppression conditions are also speculated to support the growth of EL-4 cells. A similar pattern of lymphocytopenia in the blood has also been seen in almost all cancer patients [13]. There is a question as to why hypothermia and anemia are related to the onset of cancer in humans. We previ- ously proposed the possibility that severe and continuous stress is associated of this phenomenon, namely, severe stress induces hypothermia, hypoxia and hyperglycemia [29,30]. Both sympathetic nerve activation and gluco- corticoid secretion induce such internal environment. For a short span of life, these internal conditions are beneficial for the stimulation of the glycolysis pathway of energy production [13,26]. As a result, prompt force of white muscle fiber increases and person is able to escape from emergency. If stress continues for a long time, however, such condition suppresses oxidative phosphorylation in the mitochondria and results in cer- tain diseased conditions and the onset of cancer. In the present murine model, hyperglycemia was not seen in tumor-bearing mice. However, this result might be an outcome from the leakage of glucose from the urine. This animal model did not contain the onset stage of cancer but only the maintenance of cancer growth. This notion is alternatively possible. At the last stage of cancer, hypothermia might also be induced by the conditions of cachexia. It was reported that TNFα production is related to the formation of this internal conditions [31,32]. In other words, the cause of hypothermia is different between the onset stage and the late stage. Other changes in the tested parameters include a slight increase in the level of lactate and β-ketone. Another speculation is that there is an increase of the glycolysis pathway and/or a decrease of the oxidative phosphoryla- tion pathway. In humans, it is known that many cancer patients show acidosis due to the increase of glycolysis [33,34] and some cancer patients show increased levels of ketone bodies [35,36]. 5. CONCLUSION It is concluded that hypothermia, anemia (hypoxia) and lymphocytopenia (immunosuppression) are essential for growth of cancer in animals and that they are com- mon signs seen in cancer patients and tumor-bearing mice. 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