International Journal of Organic Chemistry
Vol.3 No.1A(2013), Article ID:30716,6 pages DOI:10.4236/ijoc.2013.31A004

Solid Phase Synthesis of 3,4,5-Trisubstituted-1,2,4-Triazoles Derivatives from the Resin-Bound Acylhydrazines

Rongjian Xie, Xingang Liu, Chao Mai, Zhanxiang Liu*

Department of Chemistry, Zhejiang University (Xixi Campus), Hangzhou, China

Email: *

Copyright © 2013 Rongjian Xie et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received January 18, 2013; revised March 9, 2013; accepted April 13, 2013

Keywords: Resin-Bound Acylhydrazine; Solid-Phase Synthesis; 1,2,4-Triazoles


An extension of our methodology on solid-phase synthesis of 3,4,5-trisubstituted-1,2,4-triazoles under mild conditions has been developed. Firstly, the resin-bound acylhydrazine is reacted with orthoesters to provide resin-bound 1,3,4- oxadiazoles. Secondly, condensation of 1,3,4-oxadiazoles resin with the corresponding arylamines hydrochloride to form the resin-bound triazoles. 3,4,5-Trisubstituted-1,2,4-triazoles derivatives were obtained from resin-bound acylhydrazines in several steps providing 78% - 87% overall yields and excellent purity. The advantages of this method include straightforward operation and high yield and purity of the products.

1. Introduction

Substituted 1,2,4-triazoles play an important role in organic chemistry due to their presence as key structural units in pharmaceuticals and biologically active heterocycles [1]. Compounds possessing the 1,2,4-triazole moiety have been reported to display antimicrobial activity [2], antifungal [3], antibacterial [4,5], anti-inflammatory [6] and antituberculosis [7] activities. They also can be used as peptidomimetic moieties and as hydrogen bond acceptors [8,9] or as amide bond isostere for the design of receptor ligands in order to enhance their pharmacokinetic properties [10]. It is also well established that various derivatives of 1,2,4-triazole can be used for treating non-Hodgkin’s lymphoma [11]. Moreover, multiply substituted 1,2,4-triazoles are an important common structural motifs with wide applications in coordination chemistry [12,13] and material chemistry [14-16]. 3,4,5-Trisubstituted 1,2,4-triazoles also act as intermediates in the synthesis of many drugs available in the market such as maraviroc (UK-427,857) [17], triazolam [18] and sitagliptin (MK-0431) [19].

In view of their great significance and useful applications, various synthetic methodologies of multiply substituted 1,2,4-triazole derivatives have been developed [20]. Generally 1,2,4-triazole derivatives are synthesized by the cyclodehydration of N-acylamidrazones [21,22]. Several types of intermediates such as activated amide derivatives (chloromethylene amides, imidates, thioamides, thioimidates, and imidoylbenzotriazoles), amidrazones, N’-acetyl-N,N-dimethylhydrazonamides, oxadiazoles, N-nitrosoamidines, or arylphosphazoanilides can lead to N-acylamidrazones [20]. For instance, a recently described method involves the use of N, N-dimethyl formamide dimethyl acetals, yielding triazoles in a one-pot reaction with good yields [23]. Johansson and coworkers have developed a method where triazoles can easily be prepared from a range of N-substituted acetamides and hydrazides through the reaction with oxalylchloride [24]. The most commonly employed approach entails the direct condensation of thioamides with hydrazides [25], but this was not appealing because these starting materials are not commercially available in sufficient diversity. 1,2,4-Triazole derivatives were also synthesized directly from amidrazones and anhydrides using acetic acid or diethyl ether as a solvent [26,27].

One of the most important methods leading to the multiply substituted 1,2,4-triazole system involved the recyclization reactions of 1,3,4-oxadiazoles with primary amines because oxadiazoles are more stable precursors of N-acylamidrazones than most of the activated amide derivatives. This methodology has been preferably adapted in recent years [28-33]. Very recently, protic ionic liquids as dual solvent-catalysts have been successfully used to promote reactions of organoamines with oxadiazoles to afford hindered 1,2,4-triazoles [34].

Many of the solution phase methodologies described above possess important drawbacks such as long reaction times, poor yields and exhaustive purification protocols. The large number of examples we found both in academic and patent literature convinced us that 1,2,4-triazoles core can be considered as a privileged substructure of choice for the design of new potentially bioactive compounds. In the process of drug development and/or lead structure optimization it is most welcome to use a method, which allows easy access to diverse compounds. Solid phase organic synthesis offers the opportunity for the development of novel methodologies for construction of libraries of small heterocyclic compounds [35-37]. In this regard, we have investigated the syntheses of various drug-like heterocycles on polystyrene supports in order to facilitate the automated parallel synthesis of discrete compound libraries. Nevertheless, there are only a few reports in the literature concerning solid phase synthesis for 1,2,4-triazoles [38-40]. So, a facile preparation 1,2,4- triazoles for lead discovery is highly desirable. In this context we have foreseen that the use of new solid phase strategies to obtain 1,2,4-triazoles could overcome some of the deficiencies observed in previous synthetic protocols. We present a novel solid phase procedure that can be conducted in parallel form. In continuation of our particular interest in solid-phase synthesis of heterocycles from resin-bound acylhydride [41-45], herein, we describe new method for solid phase synthesis of 1,2,4-triazoles derivatives based on resin-bound acylhydrazines.

2. Results and Discussions

Scheme 1 shows the solid phase synthetic route of 1,2, 4-triazole derivatives. We have prepared resin-bound acylhydrazine 1 from the Merrifield resin according to our method previously reported [41]. The acylhydrazine resin 1 was reacted with excess orthoesters 2 catalyzed by alum [46] to give the corresponding resin-bound 1,3, 4-oxadiazoles 3. After condensation of the corresponding aniline hydrochloride with resin 3 in pyridine, the resinbound triazoles 4 were obtained. The desired 1,2,4-triazole derivatives 5 were released at TFA/DCM cleavage for resin 4 in high yield and purity and characterized by the spectroscopic methods. The results are summarized in Table 1. The progress of the reaction was monitored by Fourier transform-infrared (FT-IR) spectroscopy. When the acylhydrazine resin 1 was reacted with orthoesters in the presence of Alum, the formation of resin-bound 1,3,4-oxadiazoles 3 was amenable to KBr FTIR monitoring (i.e., disappearance of the C=O stretch at 1630 cm−1 and N-H stretch at 3429, 3318 cm−1 and the appearance of a new C=N stretch at 1590 cm−1). Resin 3 was then treated with various arylamines hydrochloride in pyridine to give the triazoles intermediate 4 which according to FTIR analysis shows a C-N stretch at 1515 cm−1. When the resin 4 was cleavaged by TFA/DCM, the product 5 was obtained in good yields and high purity.

Scheme 1. Reagents and conditions (a) KAl(SO4)2·12H2O (40 mmol%), reflux, 10 h; (b) R2NH2·HCl, pyridine, reflux 12 h; (c) TFA/CH2Cl2 (1/4). r. t. 1 h.

Table 1. Solid-phase synthesis of 1,2,4-triazole derivatives.

aYield of crude product based on the loading of acylhydrazine resin 1; bDetermined by HPLC analysis (area %); cThe triazole product was not obtained, we got the 4-(5-propyl-1,3,4-oxadiazol-2-yl)phenol.

To illustrate the versatility of this methodology, various commercially available orthoesters and amines analogues (Table 1) were used to prepare triazole resin 4. All the orthoesters used in reaction can offer the corresponding oxadiazole resin 3. While for electron-rich aromatic amines, both the yield and purity of triazoles are good consisting with the electronic effects observed in traditional solution phase synthesis. But when the electron-poor arylamines were used, such as p-NO2 and p-CF3 substituted anilines, the corresponding triazoles were not obtained. After cleavage by TFA/DCM, we got the 4-(5-propyl-1,3,4-oxadiazol-2-yl)phenol which is the corresponding product cleavaged from oxadiazole resin 3 (Table 1, entries 7 and 8). Using this methodology, we have synthesized a representative set of 1,2,4-triazole-3- thiones derivatives 5 (Table 1) in 76% - 89% overall yield, indicating an good yield for each step of the reaction.

In summary, we have developed a new strategy for the preparation of 3,4,5-trisubstituted 1,2,4-triazoles via solid phase synthesis. This process provided a useful method for the preparation of diverse 1,2,4-triazoles from readily accessible reactants under mild reaction conditions. The use of resin-bound acylhydrazines in the reaction benefits the solid-phase synthetic route because it not only provides a short synthetic route to the desired products but its chemical versatility also adds to the diversity of the library. The 1,2,4-triazoles derivatives were synthesized in several steps providing 76% - 89% overall yields and excellent purity. The developed method can be applied to the preparation of libraries containing the triazole moiety for potential drug discovery. This synthetic methodology is because all the reactions were carried out under mild conditions. Further work is in progress on the solid-phase synthesis of heterocyclic compounds via the resin-bound acylhydrazines.

3. Experimental

Starting materials were obtained from commercial suppliers and used without further purification. Solvents were dried and distilled before use. Merrifield resin (100 - 200 mesh, cross-linked with 1% divinylbenzene, loading 1.95 mmol/g Cl) was purchased from commercial sources (Nankai University).

Acylhydrazine resin 1 was prepared from the Merrifield resin according to our method previously reported [41]. 1H NMR (400 MHz) and 13C NMR (100 MHz) spectra were recorded on a Bruker Avance (400 MHz) spectrometer, using DMSO-d6 as the solvent and TMS as internal standard. Mass spectroscopy data of the products were collected on an HRMS-APCI instrument or a lowresolution MS instrument using EI or ESI ionization. IR spectra were recorded on a Bruck Vector 22 spectrophotometer. High performance liquid chromatography (HPLC) was performed on an Agilent 1100 (column, Eclipse XDBC18 5 μm, 4.6 × 150 mm; mobile phase, MeOH/H2O, 80/20 (v/v); flow rate, 1.0 mL/min; detector, UV 254 nm). The samples were further purified by thinlayer chromatography (TLC) for 13C NMR.

4. A General Procedure for Synthesis of 1,2,4-Triazoles

To the mixture of the acylhydrazine resin 1 (0.5 g, loading = 1.59 mmol/g, based on N microanalysis) in orthoester (5 mL), alum KAl(SO4)2·12H2O (0.38 g, 0.8 mmol) was added. Then the mixture was stirred and heated at 100˚C for 10 h. The resin was filtered and washed with H2O (5 mL × 3), (EtOH (5 mL × 3) and CH2Cl2 (5 mL × 3) to remove contaminated species and then dried to afford the resin 3.

A mixture of resin 3, aniline hydrochloride (1.03 g, 8 mmol), and pyridine (5 mL) was refluxed for 12 h. Then it was filtered and the resin was washed with 10% HCl (5 mL × 3), H2O (10 mL × 3), EtOH (5 mL × 3) and CH2Cl2 (5 mL × 3) and dried to affored the resin 4b.

The resin 4b was well swallen in 3 mL CH2Cl2, then 0.5 mL TFA was added. The mixture was stirred at room temperature for 1 h and then filtered. The resin was washed completely with EtOH (5 mL × 3) and acetone (5 mL × 3). The filtrate was combined to afford the crude product 5 by evaporation.

All compounds gave satisfactory 400 M 1H NMR, IR, and HRMS spectra.

4-(4-(p-tolyl)-4H-1,2,4-triazol-3-yl)phenol (5a)

mp 280˚C - 281˚C; 1H NMR δ = 9.85 (s, 1H), 8.71 (s, 1H), 7.31 (d, J = 7.2 Hz, 2H), 7.23 (m, 4H), 6.74 (d, J = 8.4 Hz, 2H), 2.36 (s, 3H). 13C NMR 159.0, 152.7, 145.5, 139.1, 132.5, 130.5, 130.2, 126.2, 117.6, 115.7, 21.0. IR 2603, 1610, 1513, 1446, 1276, 1247, 1181, 845, 813 cm−1. Calcd for C15H13N3O, 251.1059. Found: 251.1052

4-(4-phenyl-4H-1,2,4-triazol-3-yl)phenol (5b)

mp 241˚C - 243˚C; 1H NMR δ = 9.97 (s, 1H), 8.81 (s, 1H), 7.51 (m, 3H), 7.36 (m, 2H), 7.19 (d, J = 8.4 Hz, 2H), 6.74 (d, J = 8.8 Hz, 2H). 13C NMR 159.0, 152.7, 145.5, 135.0, 130.3, 130.1, 129.5, 126.4, 117.3, 115.7. IR (KBr): 3094, 2591, 1612, 1506, 1438, 1388, 1281, 1243, 834, 769, 691 cm1; Calcd for C14H11N3O, 237.0902, Found: 237.0900



mp 282˚C - 283˚C. 1H NMR δ = 9.88 (s, 1H), 7.53 (m, 3H), 7.38 (m, 2H), 7.12 (m, 2H), 6.68 (m, 2H), 2.19 (s, 3H). 13C NMR 158.8, 153.5, 152.0, 135.2, 130.3, 129.8, 129.7, 127.9, 118.2, 115.6, 11.3. IR 2591, 1610, 1539, 1512, 1439, 1411, 1272, 1240, 845, cm1; Calcd for C15H13N3O, 251.1059. Found: 251.1052.



mp 279˚C - 280˚C; 1H NMR δ = 9.93 (s, 1H), 7.33 (d, J = 8.0 Hz, 2H), 7.25 (d, J = 8.0 Hz, 2H), 7.15 (d, J = 8.0 Hz, 2H), 6.70 (d, J = 8.0 Hz, 2H), 2.36 (s, 3H), 2.18 (s, 3H). 13C NMR; 158.8, 153.5, 152.1, 139.5, 132.5, 130.8, 130.0, 128.6, 127.6, 118.1, 115.6, 21.1, 11.2. IR 3321, 3147, 2943, 2589, 1533, 1499, 1442, 1286, 1243, 835, 690 cm1. Calcd for C16H15N3O: 265.1215. Found: 265.1211.


yl)phenol (5e)

mp 254˚C - 256˚C. 1H NMR δ = 9.76 (s, 1H), 8.17 (d, J = 8 Hz, 1H), 8.10 (d, J = 8 Hz, 1H), 7.74 (m, 1H), 7.70 (m, 1H), 7.62 (m, 1H), 7.54 (m, 1H), 7.09 (m, 3H), 6.56 (d, J = 8.4 Hz, 2H), 2.05 (s, 3H). 13C NMR 158.7, 154.1, 152.7, 134.1, 131.2, 130.7, 129.6, 129.0, 128.7, 127.5, 127.2, 126.3, 121.5, 118.3, 115.6, 115.5, 10.8. IR (KBr, cm1): 3146, 2925, 2618, 1582, 1513, 1437, 1290, 1239, 1173, 838, 702. Calcd for C19H15N3O, 301.1215. Found: 301.1216.



mp 234˚C - 235˚C; 1H NMR (400 MHz DMSO-d6): δ = 9.83 (s, 1H), 7.33 (d, J = 8 Hz, 2H), 7.24 (d, J = 8 Hz, 2H), 7.14 (d, J = 8.4 Hz, 2H). 6.67 (d, J = 8.4Hz, 2H), 2.48 (t, J = 7.6 Hz, 2H), 2.37 (s, 3H), 1.55 (m, 2H), 0.84 (t, J = 7.4 Hz, 3H). 13CNMR δ = 158.8, 155.1, 153.5, 139.5, 132.5, 130.7, 129.8, 127.8, 118.2, 115.6, 26.9, 21.1, 20.3, 14.0. IR 3423,2968, 1514, 1467, 1282, 828 cm1; Calcd for C18H19N3O, 293.1528. Found: 293.1530.

4-(5-propyl- 1,3,4- oxadiazol-2-yl)phenol (5g, 5h)

mp 152˚C ~ 153˚C. 1H NMR δ = 10.27(s, 1H), 7.79 (d, J = 8.8 Hz, 2H), 6.93 (d, J = 8.8 Hz, 2H), 2.84 (t, J = 7.6 Hz, 2H),1.75(m, 2H), 0.95 (t, J = 7.6 Hz, 3H).13C NMR δ = 166.2, 164.3, 160.9, 128.6, 116.5, 114.7, 26.7, 19.8, 13.7 IR 3426,2971, 1580, 1505, 1435, 1282, 1236, 1172, 844, 741, 698 cm1; Calcd for C11H12N2O2, 204.0899. Found: 204.0898.

5. Acknowledgements

The authors are grateful for the financial support from the National Natural Science Foundation of China No. 20802068 and Natural Science Foundation of Zhejiang province No. Y4110100.


  1. A. Curtis and N. Jennings, “1,2,4-Triazoles,” In: A. R. Katritzky, C. A. Ramsden, E. F. V. Scriven and R. J. K. Taylor, Eds., Comprehensive Heterocyclic Chemistry III, Vol. 5, Elsevier Ltd., New York, 2008, pp. 159-209.
  2. K. D. Patel, B. D. Mistry and K. R. Desai, “Synthesis and Antimicrobial Activity of 1,2,4-Triazoles,” Journal of Indian Chemical Society, Vol. 79, No. 9, 2002, pp. 964-965.
  3. K. Shalini, N. Kumar, S. Drabu and P. K. Sharma, “Advances in Synthetic Approach to and Antifungal Activity of Triazoles,” Beilstein Journal of Organic Chemistry, Vol. 7, 2011, pp. 668-677. doi:10.3762/bjoc.7.79
  4. K. Colanceska-Ragenovic, V. Dimova, V. Kakurinov, D. G. Molnar and A. Buzarovska, “Synthesis, Antibacterial and Antifungal Activity of 4-Substituted-5-Aryl-1,2,4- Triazoles,” Molecules, Vol. 6, No. 10, 2001, pp. 815-824. doi:10.3390/61000815
  5. B. S. Holla, K. N. Poorjary, B. S. Rao and M. K. Shivananda, “New Bis-Aminomercaptotriazoles and BisTriazolothia-Diazoles as Possible Anticancer Agents,” European Journal of Medicinal Chemistry, Vol. 37, No. 6, 2002, pp. 511-517. doi:10.1016/S0223-5234(02)01358-2
  6. G. Turan-Zitouni, Z. A. Kaplancikli, A. Özdemir, P. Chevallet, H. B. Kandilci and B. Gümüsel, “Studies on 1,2, 4-Triazole Derivatives as Potential Anti-Inflammatory Agents,” Archiv der Pharmazie, Vol. 340, No. 11, 2007, pp. 586-590. doi:10.1002/ardp.200700134
  7. G. R. Jadhav, M. U. Shaikh, R. P. Kale, M. R. Shiradkar and C. H. Gill, “SAR Study of Clubbed [1,2,4]-Triazolyl with Fluorobenzimidazoles as Antimicrobial and Antituberculosis Agents,” European Journal of Medicinal Chemistry, Vol. 44, No. 7, 2009, pp. 2930-2935. doi:10.1016/j.ejmech.2008.12.001
  8. S. Borg, G. Estenne-Bouhtou, K. Luthman, I. Csöregh, W. Hesselink and U. Hacksell, “Synthesis of 1,2,4-Oxadiazole-1,3,4-Oxadiazoleand 1,2,4-Triazole-Derived Dipeptidomimetics,” The Journal of Organic Chemistry Vol. 60, No. 10, 1995, pp. 3112-3120. doi:10.1021/ jo00115a029
  9. C. Chen, R. Dagnino, C. Q. Huang, J. R. McCarthy and D. E. Grigoriadis, “1-Alkyl-3-Amino-5-Aryl-1H-[1,2,4]Triazoles: Novel Synthesis via Cyclization of N-Acyl-S-Methylisothioureas with Alkylhydrazines and Their Potent Corticotropin-Releasing Factor-1 (CRF1) Receptor Antagonist Activities,” Bioorganic & Medicinal Chemistry Letters, Vol. 11, No. 24, 2001, pp. 3165-3168. doi:10.1016/S0960-894X(01)00657-6
  10. S. K. Thompson, A. M. Eppley, J. S. Frazee, M. G. Darcy, R. T. Lum, T. A Tomaszek, L. A. Ivanoff, J. F. Morris, E. J. Sternberg, D. M. Lambert, A. V. Fernandez, S. R. J. Petteway, T. D. Meek, B. W. Metcalf and J. G. Gleason, “Synthesis and Antiviral Activity of a Novel Class of HIV-1 Protease Inhibitors Containing a Heterocyclic P1’- P2’ Amide Bond Isostere,” Bioorganic & Medicinal Chemistry Letters, Vol. 4, No. 20, 1994, pp. 2441-2446. doi:10.1016/S0960-894X(01)80406-6
  11. F. Kevin, “Triazole Derivatives and Their Preparation and Method for Treating Non-Hodgkin’s Lymphoma,” US Patent No. 2006-808341, 2007.
  12. M. H. Klingele and S. Brooker, “The Coordination Chemistry of 4-Substituted 3,5-Di(2-Pyridyl)-4H-1,2,4-Triazoles and Related Ligands,” Coordination Chemistry Reviews, Vol. 241, No. 1-2, 2003, pp. 119-132. doi:10.1016/S0010-8545(03)00049-3
  13. G. Aromí, L. A. Barrios, O. Roubeau and P. Gamez, “Triazoles and Tetrazoles: Prime Ligands to Generate Remarkable Coordination Materials,” Coordination Chemistry Reviews, Vol. 255, No. 5-6, 2011, pp. 485-546. doi:10.1016/j.ccr.2010.10.038
  14. X. W. Chen, C. Y. Liu, T. H. Jen, S. A. Chen and S. Holdcroft, “Synthesis and Characterization of a Fullerene Bearing a Triazole Group,” Chemistry of Materials, Vol. 19, No. 20, 2007, pp. 5194-5199. doi:10.1021/cm071686y
  15. Z. Y. Zhou, X. W. Chen and S. Holdcroft, “Stabilizing Bicontinuous Nanophase Segregation in πCP-C60 DonorAcceptor Blends,” Journal of the American Chemical Society, Vol. 130, No. 35, 2008, pp. 11711-11718. doi:10.1021/ja802021z
  16. P. L. Wu, X. J. Feng, H. L. Tam and K. W. Cheah, “Efficient Three-Photon Excited Deep Blue Photoluminescence and Lasing of Diphenylamino and 1,2,4-Triazole Endcapped Oligofluorenes,” Journal of the American Chemical Society, Vol. 131, No. 3, 2009, pp. 886-887. doi:10.1021/ja806703v
  17. S. J. Haycock-Lewandowski, N. J. Mawby, A. Wilder and J. Ahman, “Development of a Bulk Enabling Route to Maraviroc (UK-427,857), a CCR-5 Receptor Antagonist,” Organic Process Research & Development, Vol. 12, No. 6, 2008, pp. 1094-1103. doi:10.1021/op8000614
  18. S. Fustero, J. Gonzalez and C. Del Pozo, “1,4-Benzodiazepine N-Nitrosoamidines: Useful Intermediates in the Synthesis of Tricyclic Benzodiazepines,” Molecules, Vol. 11, No. 8, 2006, pp. 583-588. doi:10.3390/11080583
  19. K. B. Hansen, J. Balsells, S. Dreher, Y. Hsiao, M. Kubryk, M. Palucki, N. Rivera, D. Steinhuebel, J. D. Armstrong III, D. Askin and E. J. Grabowski, “First Generation Process for the Preparation of the DPP-IV Inhibitor Sitagliptin,” Organic Process Research & Development, Vol. 9, No. 5, 2005, pp. 634-639. doi:10.1021/op0500786
  20. A. Moulin, M. Bibian, A. L. Blayo, S. E. Habnouni, J. Martinez and J. A. Fehrentz, “Synthesis of 3,4,5-Trisubstituted-1,2,4-Triazoles,” Chemical Reviews, Vol. 110, No. 4, 2010, pp. 1809-1827. doi:10.1021/cr900107r
  21. N. I. Korotkikh, A. V. Kiselev, A. V. Knishevitsky, G. F. Raenko, T. M. Pekhtereva and O. P. Shvaika, “Recyclization of 1, 3, 4-Oxadiazoles and Bis-1,3,4-Oxadiazoles into 1,2,4-Triazole Derivatives. Synthesis of 5-Unsubstituted 1,2,4-Triazoles,” Chemistry of Heterocyclic Compounds, Vol. 41, No. 7, 2005, pp. 866-871. doi:10.1007/s10593-005-0240-2
  22. N. A. Meanwell, J. L. Romine, M. J. Rosenfeld, S. W. Martin, A. K. Trehan, J. J. K. Wright, M. F. Malley, J. Z. Gougoutas, C. L. Brassard, J. O. Buchanan, M. E. Federic, J. S. Fleming, M. Gamberdella, K. S. Hartl, G. B. Zavoico and S. M. Seiler, “Nonprostanoid Prostacyclin Mimetics 5. Structure-Activity-Relationships Associated with [3-[4- (4,5-Diphenyl-2-Oxazolyl)-5-Oxazolyl] Phenoxy] Acetic Acid,” Journal of Medicinal Chemistry, Vol. 36, No. 24, 1993, pp. 3884-3903. doi:10.1021/jm00076a018
  23. M. J. Stocks, D. R. Cheshire and R. Reynolds, “Efficient and Regiospecific One-Pot Synthesis of Substituted 1,2, 4-Triazoles,” Organic Letters, Vol. 6, No. 17, 2004, pp. 2969-2971. doi:10.1021/ol048863a
  24. J. Lindström and M. H. Johansson, “Synthesis of 3-Aryl- 5-Methyl 4-Substituted [1,2,4] Triazoles,” Synthetic Communications, Vol. 36, No. 15, 2006, pp. 2217-2229. doi:10.1080/00397910600638994
  25. A. J. Duplantier, E. L. Bachert, J. B. Cheng, V. L. Cohan, T. H. Jenkinson, K. G. Kraus, M. W. McKechney, J. D. Pillar and J. W. Watson, “SAR of a Series of 5,6-Dihydro-(9H)-Pyrazolo [3,4-c]-1,2,4-Triazolo[4,3-Alpha]Pyridines as Potent Inhibitors of Human Eosinophil Phosphodiesterase,” Journal of Medicinal Chemistry, Vol. 50, No. 2, 2007, pp. 344-349. doi:10.1021/jm060904g
  26. B. Modzelewska-Banachiewicz, J. Banachiewicz, A. Chodkowska, E. Jagiello-Wojtowicz and L. Mazur, “Synthesis and Biological Activity of New Derivatives of 3-(3,4-Diaryl-1,2,4-Triazole-5-yl)Propenoic Acid,” European Journal of Medicinal Chemistry, Vol. 39, No. 10, 2004, pp. 873-877. doi:10.1016/j.ejmech.2004.07.002
  27. B. Modzelewska-Banachiewicz, M. Ucherek, M. Zimecki, J. Kutkowska, T. Kaminska, B. Morak-Mlodawska, R. Paprocka, M. Szulc, G. Lewandowski, J. Marciniak and T. Bobkiewicz-Kozlowska, “Reactions of N3-Substituted Amidrazones with Cis-1,2-Cyclohexane Dicarboxylic Anhydride and Biological Activitiesof the Products,” Archiv der Pharmazie, Vol. 345, No. 6, 2012, pp. 486-494. doi:10.1002/ardp.201100333
  28. S. Olson, S. D. Aster, K. Brown, L. Carbin, D. W. Graham, A. Hermanowski-Vosatka, C. B. LeGrand, S. S. Mundt, M. A. Robbins, J. M. Schaeffer, L. H. Slossberg, M. J. Szymonifka, R. Thieringer, S. D. Wright and J. M. Balkovec, “Adamantyl Triazoles as Selective Inhibitors of 11 Beta-Hydroxysteroid Dehydrogenase Type 1,” Bioorganic & Medicinal Chemistry Letters, Vol. 15, No. 19, 2005, pp. 4359-4362. doi:10.1016/j.bmcl.2005.06.040
  29. M. A. García, S. Martín-Santamaría, M. Cacho, F. M. Llave, M. Julian, A. Martínez, B. Pascual-Teresa and A. Ramos, “Synthesis, Biological Evaluation, and ThreeDimensional Quantitative Structure-Activity Relationship Study of Small-Molecule Positive Modulators of Adrenomedullin,” Journal of Medicinal Chemistry, Vol. 48, No. 12, 2005, pp. 4068-4075. doi:10.1021/jm050021+
  30. M. Saitoh, J. Kunitomo, E. Kimura, H. Iwashita, Y. Uno, T. Onishi, N. Uchiyama, T. Kawamoto, T. Tanaka, C. D. Mol, D. R. Dougan, G. P. Textor, G. P. Snell, M. Takizawa, F. Itoh and M. Kori, “2-{3-[4-(Alkylsulfinyl)Phenyl]- 1-Benzofuran-5-yl}-5-Methyl-1,3,4-Oxadiazole Derivatives as Novel Inhibitors of Glycogen Synthase Kinase-3 Beta with Good Brain Permeability,” Journal of Medicinal Chemistry, Vol. 52, No. 20, 2009, pp. 6270-6286. doi:10.1021/jm900647e
  31. A. Brown, D. Ellis, D. Pearce, M. Ralph and N. Sciammetta, “Aryloxypyrazines as Highly Selective Antagonists of Oxytocin,” Bioorganic & Medicinal Chemistry Letters, Vol. 19, No. 10, 2009, pp. 2634-2636. doi:10.1016/j.bmcl.2009.03.160
  32. T. Sugane, T. Tobe, W. Hamaguchi, I. Shimada, K. Maeno, J. Miyata, T. Suzuki, T. Kimizuka, A. Kohara, T. Morita, H. Doihara, K. Saita, M. Aota, M. Furutani, Y. Shimada, N. Hamada, S. Sakamoto and S. Tsukamoto, “Synthesis and Biological Evaluation of 3-Biphenyl-4-yl- 4-Phenyl-4H-1,2,4-Triazoles as Novel Glycine Transporter 1 Inhibitors,” Journal of Medicinal Chemistry, Vol. 54, No. 1, 2011, pp. 387-391. doi:10.1021/jm101031u
  33. P. H. J. Carlsen and K. B. Jørgensen, “Synthesis of Unsymmetrically Substituted 4H-1,2,4-Triazoles,” Journal of Heterocyclic Chemistry, Vol. 31, 1994, pp. 805-807.
  34. X. F. Chen, R. Liu, Y. Xu and G. Zou, “Tunable Protic Ionic Liquids as Solvent-Catalysts for Improved Synthesis of Multiply Substituted 1,2,4-Triazoles from Oxadiazoles and Organoamines,” Tetrahedron, Vol. 68, 2012, pp. 4813-4819. doi:10.1016/j.tet.2012.03.114
  35. P. Seneci, “Solid Phase Synthesis and Combinatorial chemistry,” Wiley InterscienceJohn, Wiley & Sons, New York, 2000.
  36. R. E. Dolle, “Comprehensive Survey of Combinatorial Library Synthesis,” Journal Combintorial Chemistry, Vol. 6, No. 5, 2004, pp. 623-679. doi:10.1021/cc0499082
  37. P. H. Toy and Y. Lam, “Solid-Phase Organic Synthesis Concepts, Strategies, and Applications,” John Wiley & Sons, Inc., Hoboken, 2012.
  38. A. R. Katritzky, M. Qi, D. Feng, G. Zhang, M. C. Griffith and K. Watson, “Synthesis of 1,2,4-Triazole-Functionalized Solid Support and Its Use in the Solid-Phase Synthesis of Trisubstituted 1,2,4-1,2,4-Triazoles,” Organic Letters, Vol. 1, No. 8, 1999, pp. 1189-1191. doi:10.1021/ol990186a
  39. S. D. Larsen and B. A. DiPaolo, “Traceless Solid-Phase Synthesis of 1,2,4-Triazoles Using a Novel Amine Resin,” Organic Letters, Vol. 3, No. 21, 2001, pp. 3341-3344. doi:10.1021/ol016578a
  40. Y. G. Wang, W. M. Xu and X. Huang, “Selenium-Based SafetyCatch Linker: Solid-Phase Synthesis of Vinyl-Substituted Oxadiazoles and Triazoles,” Journal of Combinatorial Chemistry, Vol. 9, No. 3, 2007, pp. 513-519. doi:10.1021/cc0700187
  41. Z. X. Liu, J. L. Zhao and X. Huang, “Solid-Phase Synthesis of 1,3,4-Oxadiazoline-5-Thione Derivatives from Resin Bound Acylhydrazines,” Bioorganic & Medicinal Chemistry Letters, Vol. 16, No. 7, 2006, pp. 1828-1830. doi:10.1016/j.bmcl.2006.01.002
  42. Z. X. Liu, Y. Y. Mu, J. Lin and Y. Y. Chen, “Traceless Solid-Phase Synthesis of 1, 2, 3-Thiadiazole Derivatives from Resin-Bound Acylhydrazine,” Synthetic Communications, Vol. 38, No. 24, 2008, pp. 4407-4414. doi:10.1080/00397910802369521
  43. Z. X. Liu, Y. Y. Mu and Z. S. Song, “Solid Phase Synthesis of 4,5-Disubstituted 1, 2, 4-Triazol-3-One Derivatives from Resin-Bound Acylhydrazines,” Journal of Chemical Research, No. 4, 2008, pp. 216-217.
  44. Z. X. Liu, Z. S. Song, J. L. Zhao and J. Y. Zheng, “SolidPhase Synthesis of 2-Arylamino-5-(4-Hydroxyphenyl)-1, 3,4-Thiadiazole Derivatives Based on Resin-Bound Acylhydrazines,” Phosphorus, Sulfur and Silicon and the Related Elements, Vol. 185, No. 11, 2010, pp. 2375-2381. doi:10.1080/10426501003664176
  45. Z. X. Liu, Y. R. Yi, J. L. Zhao and M. X. Tang, “SolidPhase Synthesis of 4,5-Disubstituted-1,2,4-Triazole-3-Thione Derivatives Based on the Resin-Bound Acylhydrazines,” Synthetic Communications, Vol. 42, No. 1, 2012, pp. 55- 61. doi:10.1080/00397911.2010.521609
  46. M. Dabiri, P. Salehi, M. Baghbanzadeh and M. Bahramnejad, “Alum (KAl(SO4)12H2O): An Efficient and Inexpensive Catalyst for the One-Pot Synthesis of 1,3,4- Oxadiazoles under Solvent-Free Conditions,” Monatshefte für Chemie-Chemical Monthly, Vol. 138, No. 12, 2007, pp. 1253-1255. doi:10.1007/s00706-007-0724-0


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