R. F. LAI ET AL.
Copyright © 2013 SciRes. ENG
and subsequently assessed the bone regenerative effect
[16]. The results revealed that the group grafted with
AXCB soaked with BMP-4 (AXCB/ rhBMP-2) had
much better and more extensive bone regeneration than
the group grafted with AXCB only, and the bone rege-
neration increased over time from 4 weeks to 12 weeks
after operation, which indicated that rhBMP-2 has sig-
nificant bone regenerative effect over time with AXCB
as a scaffold, and AXCB is probably a good carrier for
BMP-4, which can help rhBMP-2 release slowly and
work effectively. On the other hand, the AXCB was
found to be gradually degraded over time, and at 12
weeks after operation, the implanted bone was almost
completely replaced by newly regenerated bone tissue,
which showed apparent mature trabecular structure.
There were no appreciable histological signs of inflam-
mation or immune rejection of the graft.
In conclusion, the osteogenic effect of AXCB graft
soaked with rhBMP-2 is proved much better than AXCB
graft alone (without rhBMP-2, which shows no signifi-
cant difference with the autologous bone graft). Xeno-
geneic antigen-extracted pig massive cancellous bone has
shown good biocompatibility and it may potentially re-
place aut ologo us bone graf t in re pair of l arg e bone de fect s.
This study has provided a new reference for bone rege-
neration in the oral and maxillofacial region.
5. Acknowledgements
This work was supported by a grant from the Guangdong
Science and Technology Foundation (No: 2011B0807-
01053). We thank the Department of Nuclear Medicine,
the Animal Center, and the Institute of Biomedical En-
gineering at Jinan University, and Guangdong Guan-Hao
Science and Technology Development Co. Ltd for their
generous support.
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