Vol.2, No.12, 1425-1429 (2010) Health
Copyright © 2010 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/
The efficacy of recovery care with cool veil serum
Yu Yumoto, Takehiko Yukishita, Keiko Lee, Hiroyuki Kobayashi
Department of Hospital Administration, Graduate School of Medicine, Juntendo University School of Medicine, Tokyo, Japan;
*Corresponding Author: yuyumoto1@gmail.com
Received 28 September 2010; revised 18 October 2010; accepted 22 October 2010
The purpose of this study was to investigate the
efficacy of post-exercise treatment with “Cool
Veil Serum (CVS)” developed by Madre:X Co. Ltd.
(Shibuya, Tokyo) to prevent exercise-induced
muscle swelling. Calf circumferences and mus-
cle stiffness of both legs were measured in 10
healthy female. The measurements were made
on before 5-minute exercise, after the exercise,
and after 10-minute recovery care with CVS and
5-minute rest. The recovery care was applied
only on right leg of the subjects. Then, multiple
comparisons of calf circumference and muscle
stiffness on each leg among pre-exer-
cise, post-exercise, and final measurements
were carried out. Exercise caused a significant
increase in calf circumference and muscle stiff-
ness. The recovery care with CVS rapidly re-
duced post-exercise calf circumference to
pre-exercise level. Relationship between reduc-
ing muscle swelling after exercise and prevent-
ing sport injury should be explored in further
Keywords: Recovery Care; Cool Veil Serum;
Massage after Exercise; Calf Circumference;
Muscle Stiffness
Growing health consciousness in recent years is in-
spiring many people to lead an active lifestyle. Accord-
ing to the 2008 Sports Life Survey, recreationally active
individuals who engage in “moderate intensity exercise
twice a week for 30 min” for health promotion and
maintenance constituted a record-breaking 45.5% of the
population since the survey first began in 1992. In addi-
tion, the number of people participating in physical ac-
tivity and sports has risen to 56.4% [1]; indeed more
than 50 million Japanese people are currently exercising
on a weekly basis.
Meanwhile the 2008 National Health and Nutrition
Survey showed that one in two men and one in five
women aged 40-74 years are afflicted with pre-metabolic
and metabolic syndrome in Jap a n [2]. As dietary changes
and exercise programs become the cornerstones of
treatment for metabolic syndrome, the physically active
population is anticipated to increase further.
The rising exercise trend reflects a shift towards posi-
tive health pr omotion, how ever, a commensurate overall
increase in sports-related injuries and fatigue-induced
pain is expected as well. In general, athletes are more
conscious about post-exercise recovery and have easier
access to knowledgeable trainers and therapists. None-
theless among the collegiate athletes who are educated in
recovery care more so than ordinary people, only half
confess to stretching before and not after workouts [3].
Additionally, the number of certificated athlete trainers
by Japan Sports Association is only 1,356 in Japan [4],
while more than 30,000 athletic trainers belonging to
National Athletic Trainers’ Association are available in
the United States [5]. Therefore, there have been few
non-athletes exercising with certified athletic trainers in
Japan. In light of these findings, recreationally active
people are at higher risk of foregoing post-workout re-
covery care. Consequently this very paucity of aware-
ness and knowledge appears to promote sports injuries.
Despite the importance of post-workout recovery
strategies as lauded by healthcare professionals and ath-
letic trainers, simple post-exercise body care for ordinary
people are currently not available. Accordingly we
created “Cool Veil Serum (CVS)” for post-workout re-
covery care that regular people can routinely use to faci-
litate cool down and prevent fatigue by massaging into
exercised muscles. Our intention is to elevate con-
sciousness about recovery care with a handy product like
CVS that provides additional benefits of protecting the
skin, restoring vitality, and enhancing the circulation. In
the present study, we evaluated the effect of “Cool Veil
Serum” application after a workout in preventing exer-
cise-induced muscle swelling.
Y. Yumoto et al. / Health 2 (2010) 1425-1429
Copyright © 2010 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/
2.1. Cool Veil Serum (CVS)
The Body Hygiene Cool Veil Serum was created by
Madre: X Co. Ltd (Shibuya, Tokyo) to deliver a product
that at once prevents fatigue, cools working muscles, and
protects the skin when applied after physical activity.
Ingredients such as BCAA, glutamic acid, alginine, and
citric acid have been blended to hasten recovery. Men-
thol has been added to cool muscles. In addition, this
lotion has been enrich ed with skin emolliating microcol-
lagen and hyaluronic acid and rend ers the dual benefit of
nourishing the skin and improving blood and lymph flow
when applied on working muscles. Hydrangeic acid, a
compound derived from Hydrangea macrophylla var.
thunbergii, has been reported to upregulate adiponectin
expression and thereby reduce triglycerides and blood
glucose levels [6], potentially halting the genesis of me-
tabolic syndrome.
2.2. Study Participants
Ten healthy, regularly active female subjects aged
21-35 participated in the study after providing oral con-
sent. Individuals who are pregnant or possibly pregnant,
or unable to perform weight-bearing exercises were ex-
cluded from the study.
2.3. Methods
We used five steps to study t he efficacy of post -exerci se
recovery care with CVS.
1) Pre-exercise (baseline) measurements
The present study was conducted at our research in-
stitute. Baseline calf circumference and muscle stiffness
of both legs were measured before the test exercise. The
circumference of the most prominent aspect of the ga-
strocnemius muscle served as the reference body part.
The gastrocnemius circumference was measured by hand
and NEUTONE muscle hardness tester (TRY-ALL
COPORATION, TDM-NAI, Chiba, Japan) was em-
ployed to evaluate muscle stiffness. A semi-permanent
ink pen was used to create landmarks on the skin of the
gastrocnemius to allow repeated measures to be taken at
the same location each time. Final measurements were
taken after confirming the reliability of test methods
through several practice measurements.
2) Weight-bearing exercise for the lower legs
Each participant performed 150 bilateral h eel lifts in a
temperature-controlled (23C) room. A trainer supervised
the 5-min test exercise to ensure that stress load and pace
of all participants were consistent.
3) Post-exercise measurements
The gastrocnemius circumference and muscle stiff-
ness were measured immediately after the heel lift test
4) Recovery care with CVS
Following post-exercise measurements, each partici-
pant performed a 10-min self-application with CVS only
on the right leg. The left leg, served as control, was in
rest position during the right-leg recovery care. Recov-
ery care was performed as follows:
a) Apply 10 ml of CVS to right posterior calf and
thigh slowly (repeat 20 times for about 7 min).
b) Apply 10 ml of CVS to right lateral shin and thigh
slowly (repeat 10 times for about 3 min).
5) Final measurements
The final calf circumference and muscle stiffness were
measured after the 10-min self-application and 5-min
2.4. Data Analysis
Student’s t-test was used to determine pre-exercise
difference between the legs. As the leg-to-leg compari-
son model was used to determine the efficacy of test
lotion (CVS) in recovery care, our analysis thence pro-
ceeded on the assumption that baseline measurements
did not reveal striking left-right differences.
Repeated measures analysis of variance (ANOVA)
and Turkey’s method of multiple comparisons were run
to capture the differences among pre-exercise,
post-exercise, and final measurements. Statistical signi-
ficance was set at P < 0.05 for all analyses. Data analysis
was performed using a statistical software package
(SPSS Ve r. 11.5).
3.1. Results of Measurements
Table 1 summarizes the pre-exercise, post-exercise,
and final measurements of the gastrocnemius of the 10
study participants. (Table 1-1: Calf circu mference, Table
1-2: Calf stiffness)
3.2. Left-Right Pre-Exercise Differences
No significant differences were found in baseline cir-
cumference and stiffness between the legs (calf circum-
ference: p = 0.835, muscle stiffness: p = 0.884). We
therefore treated the two sides as symmetrical and con-
ducted further analysis based on these fin di n gs.
3.3. Comparisons among Pre-Exercise,
Post-Exercise, and Final Measurements
3.3.1. Calf circumference
Figure 1 depicts the means of pre-exercise, post-
Y. Yumoto et al. / Health 2 (2010) 1425-1429
Copyright © 2010 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/
Table 1-1. Right and left calf circumferences of the subjects on pre-exercise, post-exercise and final measurements (N = 10).
CVS(Right) Control(Left)
Subjects Age Pre-exercise Post-exercise FinalPre-exercise Post-exercise Final
1 19 33.0 33.2 32.4 32.8 33.4 32.6
2 24 34.3 35.7 34.1 34.8 36.3 35.5
3 28 34.4 35.0 34.2 34.0 34.6 34.2
4 28 32.9 34.0 33.5 34.0 33.8 34.0
5 28 31.0 31.0 30.4 31.5 31.5 31.5
6 35 34.5 34.9 34.4 34.6 35.2 34.7
7 31 33.5 34.1 33.6 32.0 34.0 33.4
8 31 34.5 35.5 34.8 33.2 35.0 34.7
9 31 35.2 36.6 36.2 35.5 36.9 36.6
10 31 38.2 39.7 38.1 37.4 39.4 38.4
Average 28.6 34.2 35.0 34.2 34.0 35.0 34.6
SD 4.5 1.9 2.3 2.1 1.7 2.2 2.0
Table 1-2. Right and left calf muscle stiffness of the subjects on pre-exercise, post-exercise and final measurements (N = 10).
CVS(Right) Control(Left)
Subjects Age Pre-exercise Post-exercise FinalPre-exercise Post-exercise Final
1 19 33.5 34.0 34.0 33.0 35.0 34.0
2 24 29.0 30.5 30.0 28.0 30.0 29.5
3 28 32.0 34.0 32.0 33.0 37.0 34.0
4 28 30.0 32.0 30.0 31.0 34.0 32.0
5 28 34.0 39.0 37.0 34.0 38.5 37.5
6 35 31.0 32.0 31.0 31.0 32.5 31.0
7 31 28.5 29.0 28.5 28.5 30.0 29.0
8 31 35.0 34.0 32.0 34.0 33.0 33.0
9 31 31.0 33.0 31.0 31.0 34.0 32.0
10 31 28.0 34.0 33.0 30.0 34.0 34.0
Average 28.6 31.2 33.2 31.9 31.4 33.8 32.6
SD 4.5 2.4 2.7 2.4 2.1 2.7 2.5
exercise and final circumference. Significant baseline
and post-exercise differences (left and right: p < 0.001)
confirmed that exercise caused a marked increase in calf
circumference. The mean circumference of the CVS-
applied (right) leg was significantly less compared with
the mean of post-exercise circumference (p < 0.001). In
contrast, the control (left) leg did not show a striking
difference between post-exercise and final circumference
(p = 0.090). Moreover, the final circumference of the left
leg was significantly greater than pre-exercise circumfe-
rence (p = 0.025).
3.3.2. Muscle Stiffness
The means of pre-exercise, post-exercise, and final
stiffness are evident in Figure 2. Significant lateralized
differences between pre- and post-exercise stiffness (left:
p < 0.001, right: p = 0.007) confirmed that exercise in-
duced prominent muscle stiffness. A small difference
was noted between the means of post-exercise and final
stiffness of the CVS-applied (right) leg, albeit statisti-
cally insignificant (p = 0.080). In contrast, the control
(left) leg showed a significant difference between post-
exercise and post-immobilization muscle stiffness (p =
0.035). Unlike the right leg, stiffness of the left leg re-
mained significantly greater after immobilization com-
pared with basel i ne (p = 0.02 7).
We found that recovery care with CVS rapidly re-
Y. Yumoto et al. / Health 2 (2010) 1425-1429
Copyright © 2010 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/
Figure 1. Multiple comparisons among pre-exercise, post-
exercise and final measurements of right and left calf circum-
ferences (*: p < 0.05, **: p < 0.01).
Figure 2. Multiple comparisons among pre-exercise, post-
exercise and final measurements of right and left calf muscle
stiffness (*: p < 0.05, **: p < 0.01).
stored post-exercise calf circumference to baseline level.
Exercise caused a significant increase in calf circumfe-
rence and muscle stiffness (calf circumference: right and
left: p < 0.001, muscle stiffness: left: p < 0.001, right: p
= 0.007). The main cause of increased calf circumfe-
rence was augmented blood flow and retention of tissue
fluids in the working muscles. The major factors that led
to greater stiffness included shortening of muscle tissue,
changes in tensile strength of the muscle fibers, and ac-
cumulation of blood and body fluids.
The final circumference of the CVS-applied (right)
leg reduced significantly compared with post-exercise
circumference (p < 0.001), to a near baseline level. With
respect to final stiffness, a trend towards improvement to
a near baseline level was observed when compared with
post-exercise stiffness, yet the difference was not signif-
icant (p = 0.080). We surmised that CVS application
promoted the reduction in calf circumference and muscle
stiffness by relaxing shortened muscle tissues and im-
proving circulation.
In contrast, the circumference of the control leg de-
creased to a certain level after the exercise; however, it
remained significantly greater than baseline even after
15 minutes of stopping the exercise (p = 0.025). A sig-
nificant improvement in stiffness was observed after
immobilization (p = 0.035); nevertheless the level of
stiffness remained significantly greater than baseline 15
minutes after the exercise (p = 0.027). These results
suggest that natural recovery via immobilization did not
recover stiffness to the baseline level.
Our results suggest that recovery care with CVS mar-
kedly reduces calf circumference, but the correlation
between reduced calf size and amelioration of muscle
pain and fatigue after physical activity remain unex-
plained. In this study, we evaluated the acute effects of
CVS in recovery, but further studies are warranted to
investigate the effect of recovery care in muscle pain and
fatigue one day after the workout. Several divergent
views abound regarding the efficacy of stretching and
massage for post-workout recovery. While stretching has
been promoted for years as an essential part of a fitness
program, Herbet et al. reported that post-exercise stret-
ching failed to prevent muscle pain [7,8]. Moreover,
Thacker et al. refuted the benefits of stretching before
and after a workout in preventing sports injuries [9].
Furthermore, Johnhagen et al. reported that centripetal
sports massage after physical activity did not lead to
functional recovery of the muscles [10].
In contrast, Mastubashi et al. reported that stretching
was effective in reducing muscle stiffness after a wor-
kout [11]. However, Matsubashi also cautioned that self-
stretch had no effect on muscle stiffness and that notice-
able improvement required proficiency in techniques
[11]. Our particular method of recovery care did not yield a
significant improvement in muscle stiffness; however, a
more accurate evaluation of stiffness may be obtained by
revising the methodology and conducting an assessment
after repeated intervention.
Although our results confirm the general efficacy of
recovery care with CVS, the effect of glutamic and citric
acid-containing CVS itself requires further evaluation.
For this purpose, a comparative analysis of recovery care
with vaseline is necessary in the future. A more stringent
evaluation under controlled environment is required to
eliminate the blind faith in the effectiveness of post-
exercise stretching and massage held by some of the
health care professionals, athletic trainers and therapists.
We conducted a post-workout recovery care with CVS
to investigate its effect in preventing sports injury and
hastening recovery, and measured calf circumference
Y. Yumoto et al. / Health 2 (2010) 1425-1429
Copyright © 2010 SciRes. Openly accessible at http://www.scirp.org/journal/HEALTH/
and muscle stiffness before and after the intervention.
Our results showed that recovery care with CVS induced
a significant reduction in calf circumference to
pre-exercise level.
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