Open Journal of Stomatology, 2013, 3, 281-286 OJST doi:10.4236/ojst.2013.35047 Published Online August 2013 (http://www.scirp.org/journal/ojst/) Lef1 may contribute to agenesis of the third molars in mice Takehiko Shimizu1,2, Eri Yokoi1, Takahiro Ichinosawa1, Yuri Kiguchi1, Fusae Ishida1, Takahide Maeda1,2 1Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan 2Nihon University Research Institute of Oral Science, Chiba, Japan Email: shimizu.takehiko@nihon-u.ac.jp Received 6 June 2013; revised 6 July 2013; accepted 21 July 2013 Copyright © 2013 Takehiko Shimizu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Tooth agenesis is the most common developmental anomaly of the human dentition. Epilepsy-like disor- der (EL) mice, which have a 100% incidence of agen- esis of the third molars, may be a good model for the genetic study of human tooth agenesis. Our previous congenic breeding strategy using EL mice confined a major locus for agenesis of M3, designated am3, with- in an approximately 1 Mega base pair (Mbp) interval on chromosome 3, which contains five known genes; Lef1, Hadh, Cyp2u1, Sgms2 and Papss1. The aim of this study was to identify the strongest candidate for am3 among the five genes using real-time PCR analy- sis. The tooth germs of M3 in the bud stage of EL and control mice were dissected out, and total RNA was extracted. In real-time PCR analysis, a significantly low level of expression of Lef1, which is one of the essential transcription factors for early tooth devel- opment, was observed in M3 of EL mice. In addition, a significantly low level of expression of Fgf4, which is a direct transcriptional target for LEF1 in early tooth development, was observed in M3 of EL mice. Our results suggest that the cause of M3 agenesis of EL mice may be a low level of Lef1 expression in M3 in the bud stage of EL mice. Keywords: Hypodontia; Gene Expression; EL Mice 1. INTRODUCTION Tooth agenesis, the congenital absence of one or more teeth, is one of the most common craniofacial anomalies in humans. Its prevalence varies from 1.6% to 9.6% when the third molars are not considered [1]. The most frequently missing teeth are the third molars, which are absent in around 20% of the population, followed by the mandibular second premolars and maxillary lateral inci- sors [2]. It appears as both syndromic and non-syndro- mic/isolated features. The genes SHH [3], PITX2 [4], IRF6 [5] and p63 [6] have been associated with syn- dromic tooth agenesis. Mutations in MSX1 [7], PAX9 [8], AXIN2 [9], WNT10A [10] and the ectodermal dysplasia genes EDA [11], EDAR [12] and EDARADD [13] have been associated with non-syndromic tooth agenesis. However, in the majority of cases of tooth agenesis, the causes remain unknown [14-18], implying that other genes must be involved. Inbred mice with homozygous alleles and a high de- gree of homology with human genes are frequently used in studies seeking to identify disease loci. Mice have a short life cycle that enables easy transgenerational ob- servation and can be reared under the same conditions so that environmental factors can be kept almost constant, making them useful for genetic research. The normal mouse dentition is composed of one continuously grow- ing incisor and three molars of limited growth in each quadrant. Congenital tooth agenesis is rarely observed in inbred mouse strains. However, the epilepsy-like disor- der (EL) mouse, which was developed as an animal model for the study of epilepsy [19], has a 100% inci- dence of absence of M3 without any generalized cranio- facial anomalies [20]. EL mice therefore may be a good model for the genetic study of agenesis of the third molar or other types of tooth agenesis in humans. Our previous genome-wide scan using F2 progeny from intercrosses between EL mice and the wild-type mice identified that alleles on chromosome 3 contribute to M3 agenesis in EL mice, suggesting independence from alleles of epi- lepsy [21]. Previous genes causing isolated tooth agen- esis; Msx1, Pax9, Axin2, Wnt10a, Eda, Edar and Eda- radd were clearly ruled out from the candidates for M3 agenesis in EL mice [22]. Recently, a major locus for agenesis of M3 (allele symbol am3) was defined in approximately the 1 Mbp region of chromosome 3 using a congenic breeding strat- egy [23]. The region contained five known genes; Lef1, Published Online August 2013 in SciRes. http://www.scirp.org/journal/ojst
T. Shimizu et al. / Open Journal of Stomatology 3 (2013) 281-286 282 Hadh, Cyp2u1, Sgms2 and Papss1. The purpose of this study was to identify the strongest candidate for am3 among the five genes by gene expression analysis. 2. MATERIALS AND METHODS 2.1. Mice EL mice were purchased from the Laboratory Animal Resource Bank at National Institute of Biomedical In- novation (Osaka, Japan) and MSM/Msf mice, a wild- type strain derived from Mus musculus molossinus pro- genitors, were obtained from the animal facilities at the National Institute of Genetics (Mishima, Japan). All ani- mals were maintained and used in accordance with the guidelines of the Nihon University Intramural Animal Use. The experimental protocol was approved by the Institutional Animal Experiment Committee (No. AP11 MD029). 2.2. Microdissection of Tooth Germ of M3 and Total RNA Isolation Ten EL mice and ten MSM mice were sacrificed under anesthesia on postnatal Day 3 (P3) when the tooth germ of M3 is in the bud stage. The heads were immediately embedded in Tissue-Tek OCT compound (Sakura Fi- neteck Japan, Tokyo, Japan). Serial sections of 30 μm thickness were prepared using a Leica cryostat (Leica Microsystems, Wetzlar, Germany). Sections were dehy- drated in 70% ethanol for 15 sec and stained with 0.25% toluidine blue for 15 sec. The upper and lower tooth germs of M3 were dissected out using a needle under a dissection microscope, avoiding the tissues surrounding the tooth follicle (Figure 1). A total of 40 M3s from each strain were collected and stored in RNAlaterTM RNA Stabilization Reagent (Qiagen, Tokyo, Japan) and total P3 P4 P5 MSM EL Figure 1. The third molar (M3) in EL and MSM/ms (MSM) at postnatal 3-day (P3), P4 and P5 (frontal sec- tion). *M3s were dissected out and collected from the sections for total RNA extraction. Bar indicates 100 μm. RNA from sections was isolated using an RNeasy Total RNA kit (Qiagen, Tokyo, Japan), in accordance with the manufacturer’s instructions. The quality and integrity of the RNA were checked by means of spectrophotometry and agarose-gel electrophoresis. 2.3. Reverse-Transcriptase Polymerase Chain-Reaction (RT-PCR) Total RNA (100 ng) from tooth germs of M3 of EL mice and MSM mice was reverse-transcribed with the PrimeScript® High Fidelity RT-PCR kit (Takara, Tokyo, Japan). The reverse-transcribed cDNA was amplified with nine pairs for the nine genes of the PCR primers listed in Table 1. The 130.73 - 131.69 Mbp region on chromosome 3 for am3 defined in EL congenic mice [23] contains five known genes; Lef1, Hadh, Cyp2u1, Sgms2 and Papss1, according to the information from Ensembl (http://asia.ensembl.org/index.html). In addition to these five genes, Wnt10a, which is upstream signaling mole- cule for Lef1 in early tooth development, and Fgf4 and Fgf3, which are downstream signaling molecules for Lef1 [24], were included in the analysis. Glyceralde- hyde-3-phosphate dehydrogenase (Gapdh) was used as control. cDNAs were amplified by 30 cycles at 95˚C for 30 sec, 60˚C for 30 sec, and 72˚C for 30 sec in the Ge- neAmp® PCR System 9700 (Applied Biosystems) and were analyzed by agarose gel electrophoresis. Products spanned at least one intron, so that cDNA products could be distinguished from potential genomic DNA products. The presence/absence and intensity of the PCR products for each gene were compared using the KODAK Mo- lecular Imaging System (Kodak). 2.4. Quantitative Real-Time PCR PCR amplification of cDNA was performed using Thermo Table 1. Primer sets for RT-PCR and real-time PCR. Gene Forward/ Reverse Primer (5’-3’) Size (bp) Lef1 Hadh Cyp2u1 Sgms2 Papss1 Wnt10a Fgf4 Fgf3 Gapdh aaggcgatccccagaaggag agggtgttctctggccttgt ttgcgctccatgtcctcctc gactctcctcaattcccttc ggctgaagtgttcagtgacc cgtatgcaaactcctcgatg tcgaccgggtcaaatgggca gcattccgggcacaggtaac gggatgcagagagcaaccaa gtgtagcacggaatgccgtg tggagactcggaacaaagtc agcttccgacggaaagcttc gctgggcctcaaaaggcttc ctgctcatggccacgaagaa ctggccatgaacaagagagg tgccattcaccgacacgtac ggaagcccatcaccatcttc cgtggttcacacccatcaca 190 194 188 190 194 193 192 190 203 Copyright © 2013 SciRes. OJST
T. Shimizu et al. / Open Journal of Stomatology 3 (2013) 281-286 Copyright © 2013 SciRes. 283 Scientific DyNAmo SYBR Green qPCR kits (Thermo Fisher Scientific, Kanagawa, Japan). The primers for real-time RCR were the same as those used for RT-PCR. The PCR program was as follows: initial denaturation at 95˚C for 5 min, followed by 40 cycles at 95˚C for 30 sec, 60˚C for 30 sec, and 72˚C for 30 sec in the DNA Engine OPTICON® Continuous Fluorescence Detector (BioRad). Gene expression levels were normalized according to the level of Gapdh expression. Relative amounts of Gapdh mRNA in each sample were calculated from standard curves obtained by sequential dilution of total RNA pre- pared from M3 at P3. We used the Mann-Whitney test to compare the expression levels of five experiments in EL and MSM mice. OJST 3. RESULT Significant decreases in the quantity of Lef1 mRNA in M3 of EL mice compared to that of MSM mice were detected in both RT-PCR and real-time PCR analysis (Figure 2). Significantly lower level of expression of Fgf4 and Fgf3 from M3 of EL mice than that of MSM mice was detected in both RT-PCR and real-time PCR analysis (Figure 3). 4. DISCUSSION In a previous study, to determine the location of the am3 locus in vivo, we produced EL congenic strains for am3 in which the restricted interval on chromosome 3 of EL mice was replaced by a wild-type-derived homologue. The congenic mice that were either heterozygous or ho- mozygous for the wild-type-derived interval exhibited a significant decrease in the incidence of M3 agenesis. The results confined the am3 locus to an approximately 1 Mbp region flanked by AC114668.1 at 130.73 Mbp and Dkk2 at 131.69 Mbp on chromosome 3, demonstrating the five candidate genes for am3; Lef1, Hadh, Cyp 2u1, Sgms2 and Papss1 based on Ensembl information [23]. Lef1 is a cell-type-specific transcription factor that participates in the Wnt signaling pathway and Lef1 has a critical role in regulating tooth morphogenesis [25,26]. Lef−/− mouse embryos exhibited that the absence of LEF1 resulted in complete lack of tooth development [24]. LEF1 directly regulates Fgf4 gene expression, and FGF4 regulates the expression of Fgf3 in the dental mesen- chyme to mediate the critical epithelial-mesenchymal interaction [27]. In the present study, significant de- creases in the quantity of Lef1 mRNA in M3 of EL mice compared to that of MSM mice were found. In addition, a significantly lower level of expression of Fgf4 and Fgf3 from M3 of EL mice than that of MSM mice was detected, while there was no difference between EL and MSM mice in expression of Wnt10a, which is a direct upstream signaling molecule for Lef1 in the bud stage. (a) (b) Figure 2. mRNA expression of Lef1, Hadh, Cyp2u1, Sgms2 and Papss1 in the third molar (M3) in the bud stage at postnatal 3-day. (a) RT-PCR analysis; (b) Real-time PCR analysis. The relative amounts of Lef1, Hadh, Cyp2u1, Sgms2 and Papss1 mRNA were divided by that of Gapdh. Results are expressed as the means of five experiments ±SD. Significantly lower level of expression of Lef1 from M3 of EL mice than that of MSM mice was detected. *p < 0.05.
T. Shimizu et al. / Open Journal of Stomatology 3 (2013) 281-286 284 (a) (b) Figure 3. mRNA expression of Wnt10a, Fgf4 and Fgf3 in the third molar (M3) in the bud stage at postnatal 3-day. (a) RT-PCR analysis; (b) Real-time PCR analysis. The relative amounts of Wnt10a, Fgf4 and Fgf3 mRNA were divided by that of Gapdh. Results are expressed as the means of five experiments ±SD. Significantly lower level of expression of Fgf4 and Fgf3 from M3 of EL mice than that of MSM mice was detected. *p < 0.05. Interestingly, expression of these genes encoding signal- ing molecules in M3 of EL mice in the bud stage was reported to be very similar to that in the first molar (M1) in the bud stage of Lef1−/− mice. M1s in the late bud stage of Lef1−/− embryos showed absence of expression of Fgf4 and Fgf3, but showed expression of Wnt10a [24]. This resemblance with previous findings suggested that Fgf4 might not be activated by LEF1 from M3 of EL mice, similar to the failure of Fgf4 activation in M1s of Lef1−/− embryos. The decrease in Lef1 may inhibit the activation of Fgf4 and Fgf3 in M3 in the bud stage of EL mice. Fgf4 and Fgf3, which are located on chromosome 7, had been excluded from the list of potential candidates for am3 based on the results of previous linkage analysis [21]. Our previous mutation analysis for the candidate genes did not find any mutation in the coding sequence of the five candidate genes from EL mice, and suggested that gene mutation is not responsible for M3 agenesis in EL mice [23]. Polymorphism in the other region of Lef1 may be the cause of the decreased expression of Lef1 in M3 of EL mice. Other candidates for am3 including Hadh (hydroxya- cyl-coenzyme A dehydrogenase), Sgms2 (sphingomyelin synthase 2), Papss1 (3’-phosphoadenosine 5’-phospho- sulfate synthase 1) and Cyp2u1 (cytochrome P450, fam- ily 2, subfamily u, polypeptide 1), show no evidence of association with hypodontia or early odontogenesis. Hadh plays a critical role in the mitochondrial beta-oxi- dation of short chain fatty acids and the gene mutation causes familial hyperinsulinemic hypoglycemia [28]. Sgms2 catalyzes the synthesis of sphingomyelin and dia- cylglycerol from phosphatidylcholine and ceramide, and Sgms2−/− mice exhibited difficulty of this conversion [29]. Papss1 is a bifunctional enzyme with both adenosine triphosphate sulfurylase and adenosine 5’-phosphosulfate kinase activity [30]. Cyp2u1 catalyzes the hydroxylation of arachidonic acid, docosahexaenoic acid and other long chain fatty acids [31]. In present study, we detected no significant difference in mRNA expression for Hadh, Sgms2, Cyp2u1 and Papss1 in M3 between EL and wild- type mice, suggesting no association with M3 agenesis. Based on our gene expression analysis, we conclude that Lef1 is the strongest candidate for am3, although the cause of the decrease of Lef1 expression in M3 of EL mice is still unclear. Unknown genes must be involved in various types of human tooth agenesis; therefore, Lef1 might contribute to a form of tooth agenesis. Mutational analysis of LEF1 in non-syndromic tooth agenesis will Copyright © 2013 SciRes. OJST
T. Shimizu et al. / Open Journal of Stomatology 3 (2013) 281-286 285 be needed in subsequent experiments. The identification of am3 in EL mice may provide clues to understanding a new mechanism of hypodontia, especially agenesis of the third molars, in humans. In the future, it may also be possible to apply the mechanism to the field of tooth regeneration. 5. ACKNOWLEDGEMENTS This study was supported by JSPS KAKENHI Grant Number 2546 3199. REFERENCES [1] Boeira Jr., B.R. and Echeverrigaray, S. (2012) Polymor- phism in the MSX1 gene in a family with upper lateral incisor agenesis. Archives of Oral Biology, 57, 1423- 1428. doi:10.1016/j.archoralbio.2012.04.008 [2] Mostowska, A., Biedziak, B. and Jagodzinski, P.P. (2012) Novel MSX1 mutation in a family with autosomal- dominant hypodontia of second premolars and third mo- lars. 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