C. López-Fernández et al. / Natural Science 5 (2013) 15-20
Copyright © 2013 SciRes. OPEN ACC ESS
20
human sperm capacitated by multiple-tube swim-up and
Percoll gradient centrifugation. Journal of in Vitro Fer-
tilization and Embryo Transfer, 5, 119-122.
doi:10.1007/BF01131172
[14] Zini, A., Finellia, A., Phanga, D. and Jarvi, K. (2000)
Influence of semen processing technique on human sperm
DNA integrity. Urology, 56, 1081-1084.
doi:10.1016/S0090-4295(00)00770-6
[15] Bungum, M., Spano, M., Humaidan, P., Eleteri, P., Rescia,
M. and Giwercman, A. (2008) Sperm chromatin structure
assay parameters measured after density gradient centri-
fugation are not predictive for the outcome of ART. Hu-
man Reproduction, 23, 4-10.
doi:10.1093/humrep/dem353
[16] Enciso, M., Iglesias, M., Galán, I., Sarassa, J. Gosalvez,
A. and Gosalvez, J. (2011) The ability of sperm selection
techniques to remove single- or double-strand DNA da-
mage. Asian Journal of Andrology, 13, 764-768.
doi:10.1038/aja.2011.46
[17] Mortimer, D. (1991) Sperm preparation techniques and
iatrogenic failures of in vitro fertilization. Human Repro-
duction, 6, 173-176.
[18] Gosálvez, J., López-Fernández, C., Fernández, J.L., Gou-
rand, A. and Holt, W.V. (2011) Relationships between the
dynamics of iatrogenic DNA damage and genomic design
in mammalian spermatozoa from eleven species. Mo-
lecular Reproduction and Developmen, 78, 951-961.
doi:10.1002/mrd.21394
[19] Gosálvez J., López-Fernández, C. and Fernández, J.L.
(2011) Sperm Chromatin Dispersion (SCD) test: Techni-
cal aspects and clinical applications. In: Zini, A., Agarwal,
A., Eds., Sperm DNA damage: Biological and clinical
applications in male infertility and assisted reproduction.
Springer Verlag, New York, 151-170.
[20] Menezo, Jr. Y., Russo, G.L., Tosti, E., Moutassim, S. and
Benkhalifa, M. (2007) Expression profile of genes coding
for DNA repair in human oocytes using pangenomic mic-
roarrays, with a special focus on ROS linked decays.
Journal of Assisted Reproduction and Genetics, 24, 513-
520. doi:10.1007/s10815-007-9167-0
[21] Meseguer, M., Santiso, R., Garrido, N., Garcia-Herrero,
S., Remohi, J. and Fernandez, J.L. (2011) Effect of sperm
DNA fragmentation on pregnancy outcome depends on
oocyte quality. Fertility and Sterility, 95, 124-128.
doi:10.1016/j.fertnstert.2010.05.055
[22] Nuñez-Calonge, R., Caballero, P., López-Fernández, C.,
Guijarro, J.A., Fernandez, J.L., Johnston, S. and Gosalvez,
J. (2012) An improved experimental model for under-
standing the impact of sperm DNA fragmentation on hu-
man pregnancy following ICSI. Reproductive Science, 19,
1163-1168. doi:10.1177/1933719112459238
[23] Gosálvez, J., De La Torre, J., López-Fernández, C., Pe-
rez-Gutierrez, L., Ortega, L., Caballero, P. and Nunez, R.
(2010) DNA fragmentation dynamics in fresh versus fro-
zen thawed 1 plus gradient-isolated human spermatozoa.
Systems Biology in Reproductive Medicine, 56, 27-36.
doi:10.3109/19396360903515430
[24] Parmegiani, L., Cognigni, G.E., Bernardi, S., Troilo, E.,
Ciampaglia, W. and Filicori, M. (2010) “Physiologic
ICSI”: Hyaluronic acid (HA) favors selection of sper-
matozoa without DNA fragmentation and with normal
nucleus, resulting in improvement of embryo quality. Fer-
tility and Sterility, 93, 598-604.
doi:10.1016/j.fertnstert.2009.03.033
[25] Tavalaee, M., Deemeh, M.R., Arbabian, M. and Nasr-
Esfahani, M.H. (2012) Density gradient centrifugation
before or after magnetic-activated cell sorting: Which
technique is more useful for clinical sperm selection?
Journal of Assisted Reproduction and Genetics, 29, 31-38.
doi:10.1007/s10815-011-9686-6
[26] Ebner, T., Shebl, O., Moser, M., Arzt, W. and Tews, G.
(2011) Easy sperm processing technique allowing ex-
clusive accumulation and later usage of DNA-strand
break-free spermatozoa. Reproductive Biomedicine On-
line, 22, 37-43. doi:10.1016/j.rbmo.2010.09.004
[27] Ollero, M., Gil-Guzman, E., Lopez, M.C., Sharma, R.K.,
Agarwal, A., Larson, K. et al. (2001) Characterization of
subset of human spermatozoa at different stages of ma-
turation: Implications in the diagnosis and treatment of
male infertility. Human Reproduction, 16, 1912-1921.
doi:10.1093/humrep/16.9.1912
[28] Agarwal, A., Makker, K. and Sharma, R. (2008) Clinical
relevance of oxidative stress in male factor infertility: An
update. American Journal Reproductive Immunology, 59,
2-11. doi:10.1111/j.1600-0897.2007.00559.x
[29] Aitken, R.J. and De Iuliis, G.N. (2010) On the possible
origins of DNA damage in human spermatozoa. Molecu-
lar Human Reproduction, 16, 3-13.
doi:10.1093/molehr/gap059
[30] López-Fernández, C., Johnston, S.D., Fernández, J.L.,
Wilson, R.J. and Gosalvez, J. (2010) Fragmentation dy-
namics of frozen-thawed ram sperm DNA is modulated
by sperm concentration. Theriogenology, 74, 1362-1370.
doi:10.1016/j.theriogenology.2010.06.006
[31] Gosálvez, J., Cortés-Gutierrez, E., López-Fernádez C.,
Fernandez, J.L., Caballero, P. and Nunez, R. (2009)
Sperm DNA fragmentation dynamics in fertile donors.
Fertility and Sterility, 92, 170-173.
doi:10.1016/j.fertnstert.2008.05.068
[32] Calamera, J.C., Buffone, M.G., Doncel, G.F., Brugo-Ol-
medo, S., De Vincentiis, S., Calamera, M.M. et al. (2010)
Effect of thawing temperature on the motility recovery of
cryopreserved human spermatozoa. Fertility and Sterility,
93, 789-794. doi:10.1016/j.fertnstert.2008.10.021