Journal of Water Resource and Protection
Vol.6 No.7(2014), Article ID:46182,11 pages DOI:10.4236/jwarp.2014.67065

Impact of Moringa oleifera Cake Residue Application on Waste Water Treatment: A Case Study

N. Ali Eman1*, C. S. Tan2, E. A. Makky2

1Faculty of Chemical and Natural Resources Engineering, University Malaysia Pahang, Kuantan, Malaysia

2Faculty of Industrial Sciences and Technology, University Malaysia Pahang, Kuantan, Malaysia

Email: *

Copyright © 2014 by authors and Scientific Research Publishing Inc.

This work is licensed under the Creative Commons Attribution International License (CC BY).

Received 9 March 2014; revised 5 April 2014; accepted 2 May 2014


Most of water treatments require higher expenditure. Chemical coagulant requires higher cost and has some drawback after treatment such as pH changes. Moringa oleifera cake residue (MOCR) is one of the alternatives to replace chemical coagulant. A jar test apparatus was used to monitor water treatment. Water quality of Gebeng River (GR) and waste water (WW) was examined before and after treatment. Different parameters using (MOCR) was investigated. Preliminary laboratory results showed the great potential of the (MOCR) to be used in water treatment. MOCR shows an excellent reduction in turbidity (97 % was removed). The bacteria were reduced from 1.7 × 105 to 8 × 103 CFU/ml. Dissolved oxygen (DO) was improved that elevated from 1.06 ± 0.04 to 5.09 ± 0.03 mg/L. However, chemical oxygen demand (COD) and biological oxygen demand (BOD) were increased from 520.5 ± 0.71 to 865.0 ± 2.12 mg/L and from 120.5 ± 2.12 to 270.5 ± 2.12 mg/L respectively. Nevertheless, there is no significant alteration of pH, conductivity, salinity and total dissolved solid (TDS) after treatment. The iron (Fe) was fully removed while copper (Cu) and cadmium (Cd) were successfully removed up to 98%. The reduction of lead (Pb) also achieved 82.17%. Also, (MOCR) can be stored for long time up to 6 months without affecting the biological properties of MO.

Keywords:Moringa oleifera, Waste Water Treatment, Dissolved Oxygen, Turbidity, Heavy Metals, Antibacterial

1. Introduction

Waste water is the by-product from municipal, agricultural and industrial activity [1] . The conditions of the waste water without treatment will be deleterious to the organism in the river and human health [2] [3] . In the era of globalization, many water treatment processes have been invented but most of them require higher expenditure [4] [5] . This is because water treatment plants are very complex and require many stages to purify the waste water. The more complex water treatment plant means the higher the expenditure [3] -[6] .

A conventional method to treat the waste water is using chemical coagulant such as aluminium sulphate and iron salts [7] [8] . It is used as a coagulant to sediment down the particles from waste water. Nevertheless, chemical coagulants have many drawbacks when applied in water treatment [4] [8] [9] . Therefore, finding natural alternatives is of great importance. Thus, early studies have found that one of these alternatives for water treatment is Moringa oleifera (MO) [10] [11] . It was a traditional method for waste water treatment by using MO which possesses the same function with the chemical coagulant such as aluminium sulphate.

MO is the most studied of the 14 species that belong to genus Moringaceae and the scientific name of this species is MO [2] [11] . Leaves and seeds of MO are most widely used in water treatment [2] [12] . There is no significant side effect using MO in water treatment because it is reported as non-toxic and biodegradable material [2] [13] .

The natural coagulant derived from MO seed is the by-product of oil extraction process. MOCR can be used directly in waste water treatment without further preparation [14] . Many laboratory studies have proven that persuasive coagulant properties are found in MO seed [6] [12] [15] -[17] . MOCR is removing turbidity from water by pulling together floating particles, which include dirt, solids particles, and even some bacteria and fungi [4] [12] [18] . MO seed possesses antibacterial properties [19] , coagulant properties to flocculate particles [6] and acts as biosorbent for heavy metal removal [16] [20] [21] .

This study aims to investigate the performance of MOCR on different water parameters such as: BOD, COD, DO, TDS, pH, salinity and conductivity, using different concentrations of MOCR applied to waste water. In addition, the study the turbidity removal, heavy metal removal and reduction of bacterial consortium in wastewater and investigate the effect of MOCR storage time on treated water properties.

2. Materials and Methods

2.1. Experimental Site

The waste water samples were collected from two target sites which are Gebeng River (GR) and waste water (WW). Gebeng River (GR) is located at main industrial area in Kuantan, Pahang State, Malaysia, it is important to study the water quality status of this river because effluents from industrial area of Gebeng are discharged into it which deteriorating the water quality. The type of waste water (WW) used in this study is leachate and collected from landfill which is located along Jabor-Kerangau Road, District of Kuantan, Pahang State, Malaysia.

2.2. Mo Seeds Preparation

2.2.1. Oil Extraction

The soxhlet extraction method was used to extract the oil from MO [22] . About 10 g of MO seed powder were put into an extraction thimble. A 170 ml of hexane solvent was poured into a round bottom flask. After set up the soxhlet apparatus, the hexane solvent was heated for 45 min and the oil was extracted. After extraction, MOCR was collected from the thimble and dried in an oven at 50˚C for overnight. Once the oil was fully removed from the MO seed, the seed cake residue could be used in water treatment. Two samples of MOCR were used for water treatment, one of freshly prepared cake residue is called Hexane Freshly Prepared (HFP), and the second sample was stored for six months to study the effect of storage time on the performance of the cake residue in water treatment and called Hexane Long Storage (HLS). The oil was extracted from the seeds because the presence of oil in the MO seed would affect the coagulation activity and heavy metal removal [23] . The higher the oil content in MO seed, the lower the performance of the MOCR in water treatment process.

2.2.2. Stock Solution Preparation

The stock solution was prepared by adding the distilled water to MOCR in different concentrations to form a paste which possesses the coagulation activity. Different concentrations were prepared in distilled water (mg/5ml) by dissolving 50, 100 and 150 mg of the seed powder to obtain 10,000, 20,000 and 30,000 mg/L with percentage of 1%, 2% and 3%, respectively [19] .

2.3. Waste Water Treatment

A waste water treatment was performed by using a PB-700 6 Paddle Jar Test apparatus. Six beakers were labelled. About 500 ml water sample was prepared into each beaker and placed into a Jar Test apparatus. Different concentrations of stock solution was added into each beaker and operated with initial speed of 150 rpm for 2 min. Then, the speed was reduced to 50 rpm and continued for 25 min. Then water samples were left for setting for 1 hour [11] [19] . After 1 hour, clear water sample was collected into the conical flask and stored at 4˚C for further analysis.

2.3.1. Heavy Metal Removal Measurement

The heavy metal removal test was done by using a PerkinElmer AAnalyst™ 800 High-Performance Atomic Absorption Spectrometer (AAS). A series of calibration solution such as copper, iron, lead and cadmium were prepared from standard stock solution (1000 mg/L). Each of the elements was prepared with 5 different concentrations of 0.5 to 7 ppm [17] . Each water sample was diluted ten-folds before analysing by AAS.

2.3.2. Turbidity Measurement

Turbidity of water was measured by using a 2100P (HACH) turbidimeter. Turbidity of water sample was measured before and after treatment to monitor the performance of MOCR [24] .

2.3.3. Chemical Oxygen Demand (COD) Measurement

A DR2800 spectrophotometer was used to measure the COD in water samples. About 2 ml of the water sample before and after treatment were pipetted into COD reagent vials and inserted into a COD reactor. The sample was heated at 150˚C for 2 hours with a strong oxidizing agent (potassium dichromate solution) to oxidize organic matter chemically [25] .

2.3.4. Biochemical Oxygen Demand (BOD) Measurement

BOD measurements were done by using a dissolved oxygen (DO) meter. About 1L of diluted water sample was prepared by adding 1 ml of phosphate buffer, magnesium sulfate, calcium chloride and ferric chloride solution into 996 ml distilled water. About 10 ml of water sample prior to and after treatment was transferred into each BOD bottle. Then, 300 ml of diluted water was added into the BOD bottle. Besides that, the control was prepared from 300 ml diluted water in BOD bottle. The DO was measured for all samples using DO meter. After that, the diluted water was added to the flared mouth of the bottle and covered with aluminium foil to prevent evaporation of the solution. All bottles were put into the BOD incubator for 5 days at 20˚C. The DO value was measured after 5 days [26] . The BOD was much higher than the limited solubility of oxygen in water; hence, it was necessary to make a series of dilutions.

2.3.5. Conductivity, pH, Total Dissolved Solid (TDS), Dissolved Oxygen (DO) and Salinity Measurement

The conductivity, pH, TDS, DO and salinity can be examined by using a multifunction PCD650 waterproof portable meter (CyberScan Waterproof Portable pH/ORP/Conductivity Meter Eutech Instruments). The measurements before the treatment of the waste sample were carried out directly in situ, and measurements after treatment with MOCR were taken in the laboratory.

2.3.6. Antibacterial Assay for Water Sample

The antibacterial assay was performed to investigate the microbiological quality of water sample. Pour plate method was used to examine the reduction of bacteria from water sample [2] . This test were conducted prior to treatment and after the water treatment by different concentration of MOCR. A series of dilutions up to 10−6 of the water sample were made before and after treatment using plating onto the nutrient agar (NA) plate [19] . About 0.1 ml aliquot of each dilution 1 to 6 test tubes were transferred onto the NA surface. Then, evenly spread onto the agar surface by using L-shape glass rod. The NA plates were incubated at 37˚C overnight. The number of colonies grown on the plate was counted. The number of colonies before and after treatment was compared.

3. Results and Discussion

3.1. Oil Extraction

The results significantly proved that the hexane is suitable for MO seed oil extraction based on the high oil yield which is 34.77%. The result obtained was strongly agreed with [18] [23] .

3.2. Water Analysis Parameters

3.2.1. River Water

Many tests have been carried out for the GR water samples before and after treatment, using two types of MOCR (HFP and HLS). The results are shown in Table 1.

Most researchers had figured out that MOCR have the ability to remove 95% - 99% turbidity from waste water [7] [8] [11] . Previous studies also indicated that MOCR used in waste water treatment will increase the COD due to presence of organic matter [14] .

Data recorded in Table 1, revealed that the pH of the GR was not significantly changed after treatment with MOCR in both samples of cake residue from (HFP) and (HLS). The pH was in range of 6.5 - 8.5 (National Water Quality Standard for Malaysia). This proved that (MOCR) is not affecting pH in water samples which showed agreement with [6] [8] [14] [19] . The storage duration does not affect the pH as well.

Moreover, the conductivity was increased after treatment within 50 µS/cm. It was elevated from 347 ± 0.00 to 392 ± 0.00 µS/cm. TDS also increased after treatment from 307 ± 0.00 to 425 ± 0.00 ppm. The increasing of TDS was not agreed with [27] which stated that the TDS was reduced after the treatment with MOCR. This was due to the different pre-treatment steps used in the experiment. But, according to current result, the conductivity and TDS were in agreement with [8] . For salinity, it was increased from 353 ± 0.00 to 418 ± 0.00 ppm. The results were similar with the investigation carried out by [14] . Concentration of MOCR will affect the result of conductivity as indicated by [19] that conductivity increases as more MOCR is added to water. Besides that, conductivity, salinity and TDS using HFP were higher than that when HLS is applied. Thus, storage duration didn’t affect salinity, TDS and conductivity and they followed the same trend since they are related to each other [8] [14] .

The COD of GR after treatment was lower than that before treatment which was 132.0 ± 2.83 and dropped to 88.5 ± 0.71 mg/L when 1% MOCR from HFP was used and 93.3 ± 0.00 mg/L when 1% MOCR from HLS was applied (Table 1), the results agreed with [10] . However, it was in contrast with [26] results which showed a great reduction of COD after treatment, this might be due to the additional treatment steps of MOCR used. The

Table 1. GR water samples parameters before and after treatment using MOCR.

1Hexane long storage batch; 2Hexane freshly prepared batch.

COD after treatment was lower than initial COD accept the use of % MOCR were the COD was higher than initial COD. MOCR is non-toxic for water purification [14] , and it is considered as encouraging results and safe to environment and aquatic organism. The increasing of COD depends on the MOCR dosage used and MOCR concentration was directly proportional to the COD. The best concentration for the COD reduction was 1% of MOCR since it showed the highest reduction in COD value. Besides that, the storage duration was not significant, and the HFP was better in removing COD due to the fresh preparation of the seed cake.

Furthermore, BOD after treatment was dramatically reduced and the results agreed with [28] where 40% of BOD can be reduced after the MOCR treatment especially at 3% (150 mg/L of MOCR stock solution). This means that the MOCR concentration will affect the BOD after treatment. The BOD was reduced from 100.10 ± 0.00 to 41.40 ± 0.42 mg/L (after treatment with 1% HFP). This result was similar with the research of [26] [28] . However, [20] had reported that the BOD was not significantly increased or decreased after the treatment. The results of BOD using HFP were slightly better than results when HLF was applied to water samples. Meanwhile, the concentration of the MOCR was affecting the BOD, higher concentration of MOCR revealed higher BOD value, and storage duration does not influence the MOCR performance.

Based on Table 1, DO was dramatically improved after treatment, 2.58 ± 0.01 improved to 5.94 ± 0.03 mg/L (3% MOCR of HFP) and 5.02 ± 0.02 mg/L (3% MOCR of HLS). There are two factors affecting the DO results which are dosage used and storage duration of the MOCR. For the comparison of storage duration, HFP showed better results of DO than the results using HLS, the MOCR concentration is directly proportional to the DO value. Hence, it was excellent to improve the DO by using MOCR, the DO reading of HFP (3%) and HLS (3%) were in the range of 5 - 7 mg/L which considered as first class water quality according to National Water Quality Standards for Malaysia.

In addition, MOCR also plays a vital role to reduce the turbidity. The initial turbidity of GR was in the low range 67.25 ± 0.07 NTU, MOCR successfully removed 85% - 93% turbidity from the GR (Table 1). This result was totally agreed with most of the previous investigations [4] [6] [8] [11] [14] [17] [29] [30] . According to the Table 1, 3% of MOCR have removed more than 90% of turbidity which was reduced to 4.55 ± 0.02 NTU and 4.93 ± 0.02 NTU using HFP and HLS, respectively, followed by 2% of MOCR. Hence, the higher concentration of MOCR means the better coagulation properties. According to National Water Quality Standard Malaysia and [31] , the first class water and drinking water quality should have turbidity <5.0 NTU. This proved that the storage period of MOCR will not affect the turbidity removal in water treatment process.

3.2.2. Waste Water

Many tests have been carried out for the water before and after treatment for WW, using two types of MOCR (HFP and HLS). The results are shown in Table 2.

Data recorded in Table 2 exhibited that the pH of the WW was not significantly changed after treatment; this

Table 2. WW water samples parameters before and after treatment using MOCR.

1Hexane long storage batch; 2Hexane freshly prepared batch.

was due to the use of natural material for water treatment [8] . The MOCR concentration and storage duration did not have any effect on pH of treated water, the results agreed with investigation by [14] [24] [26] . Thus, there was no addition step to adjust the pH value after the treatment. Hence, MOCR was suggested for water treatment industry [2] .

There was no alteration on conductivity, TDS and salinity result after the treatment. Result from Table 2 was totally agreed with [8] . The conductivity, TDS and salinity of WW before treatment were 402 ± 1.41 µS/cm, 412 ± 0.00 ppm, and 421 ± 0.85 ppm, respectively. Based on Table 2, these parameters using 2 % MOCR was slightly higher than the results when other concentrations were applied. It was reported by [24] that when the concentration of MOCR increase the water conductivity will be decreased. However, the previous reports from [14] indicated that the MO coagulant will not influence the conductivity and TDS. Thus, the results of WW were similar with the investigation from [14] . Moreover, storage duration of MOCR did not influence these parameters in water quality.

In addition, the COD result of GR was totally different from WW, the COD of WW was dramatically increased after treatment. By using HLS the COD was increased more than the case when HFP was applied, which was increased from 520.5 ± 0.71 to 865 ± 2.83 mg/L. While by using HFP the COD was increased from 520.5 ± 0.71 to 739.5 ± 2.12 mg/L. This means the storage period will deteriorate the function of MOCR and the initial water quality is affecting the performance of MOCR since WW have more initial COD than GR as shown in Table 1 and Table 2. This is because treatment of the water sample with natural material usually will show this effect [32] , and there is no way to prevent the increasing of COD after treatment with natural source. In short, dosage of MOCR was the major factor that caused the increasing of COD after treatment [14] . BOD also exhibited the same trend of COD, the BOD after treatment was increased with the increase of MOCR dosage (Table 2), and the results are not acceptable for the drinking water standard [31] . After treatment, the BOD increased especially by using HLS (270.5 ± 2.12 mg/L), then followed by HFP (250.5 ± 4.94 mg/L). But, this result was in contrast with [20] [28] . Besides that, [26] also presented the reduction of BOD after treatment with MO, and this was due to the addition treatment steps with chemical coagulants used in the experiment.

On the other hand, DO value significantly increased after the treatment, and the effect of MOCR in WW was similar with GR. DO was improved from 1.06 ± 0.04 to 4.60 ± 0.02 mg/L (using 3% MOCR of HLS) and 5.09 ± 0.03 mg/L (applying 3% MOCR of HFP). DO results by using HFP are better than HLS performance; this proved that storage duration will affect the MOCR in water treatment. Besides that, the MOCR concentration is directly proportional to the DO value; hence, it was strongly suggested using MOCR to improve the DO value. The improvement of DO in water sample after treatment is due to oil extraction from MO seeds.

The turbidity of WW before treatment is considered as medium level with turbidity of 148.3 ± 0.42 NTU. MOCR successfully removed the turbidity from WW up to 97% (by using 3% MOCR of HFP), and this reading was proven by previous research work [4] [8] [26] [29] . According to Table 2, 1% MOCR of HLS has reduced turbidity up to 6.65 ± 0.07 NTU, followed by the 2% and 3% which are 5.08 ± 0.03 and 4.79 ± 0.02 NTU, respectively. Although the residual turbidity using HLS was similar to that when HFP is used, but, HFP showed the best coagulation effect in WW. MOCR with 1% MOCR of HFP has reduced the turbidity to 6.06 ± 0.04 NTU, followed by 2% and 3% of HFP which were 5.16 ± 0.05 and 4.19 ± 0.03 NTU, respectively. The turbidity removal result after the treatment was accepted by the [31] guideline for drinking water with <5 NTU.

3.3. Heavy Metals Removal Results

3.3.1. Heavy Metals Removal from GR

Figure 1 shows heavy metal adsorption using MOCR with GR water samples. The heavy metal binds with polyelectrolyte from MOCR to form complexes [12] , and this polyelectrolyte released after oil extraction since it is not soluble in lipid [18] [20] .

The heavy metals of Cu, Cd, Fe and Pb were high in the GR water samples. After ten-fold dilution, the concentration of these metals still more than 1 mg/L. This indicated that GR water contains a high concentration of heavy metals in the stream. MOCR can act as natural adsorbent to remove the heavy metals from water sample [4] [21] . Two factors were studied to investigate the effect of MOCR activity in heavy metal removal namely dosage of MOCR, and storage duration.

HFP showed that Cu can be removed 100% when compared with the HLS which indicated 0.012 mg/L of Cu remains after treatment with 1% MOCR, followed by the 2% and 3% which showed Cu residual of 0.007 and


Figure 1. Heavy metal removal from GR using MOCR. (a) hexane long storage batch (HLS); (b) hexane freshly prepared batch (HFP).

0.00 mg/L, respectively. This means that, the higher MOCR dosage, gives better reduction of Cu, the same trend is followed using HLS with fully reduction of Cu using 3% MOCR. According to [30] , MOCR was good to remove Cu up to 90%, the increasing of MOCR concentration revealed better reduction; similarly, [17] had reported that MOCR has ability to show adsorption of Cu. The concentration of Cu after treatment was in range of the standard drinking water from [31] .

On the other hand, Cd showed significant decreasing after the treatment with natural absorbent [18] . Obviously, more than 98 % of Cd was removed at the end of the treatment, and as shown in Figure 1, it was better than that reported by [17] which only exhibited 60% of Cd removal by MO seed. Cd removal was different with other heavy metal because the storage periods did not affect the removal action. However, higher concentration of MOCR revealed better removal of Cd, in contrast with [20] who reported that only 48% of Cd was removed from the treated water, this might be due to different method used for pre-treatment of the MO seed. [20] have used the seed powder directly without oil extraction. Cd removal is acceptable for National Water Quality Standard for Malaysia when treated with MOCR, although it was not fully removed from water samples.

In addition, Fe was not detected after the treatment. The initial amount of Fe was above 1.40 mg/L and fully removed by MOCR. This result was totally agreed with [8] study, while [20] had reported that a reduction of Fe by using MOCR was up to 92% if compared to other heavy metals. There is no difference between the concentrations of MOCR used for iron removal from water sample because there is no Fe was detected after treatment. In addition, Fe was fully removed by HLS, the level of Fe was achieved the water standards since concentration of Fe in water should be less than 0.30 mg/L (National Water Quality Standard from Malaysia). Hence, the long storage period did not deteriorate the MOCR for heavy metal removal.

Figure 1 shows the reduction of Pb was up to 80% using 3% HFP (residual Pb was 0.355 mg/L), while Pb in water standard should be less than 0.05 mg/L. Although the MOCR showed some reductions of Pb in treated water, but the result was not good enough to meet the drinking water standard [31] . In this study, the removing of Pb was within the range of previous research [20] [30] which indicated that Pb can be removed within 80% - 89% by using MO seed if compared to other natural source such as beans and peanuts.

3.3.2. Heavy Metals Removal from Waste Water (WW)

The concentrations of heavy metals in WW were higher than GR, and as shown in Figure 2, heavy metal removal efficiency of MOCR in WW was similar to GR. Removal of Pb was up to 82% in WW which comes in agreement with [30] , which indicated that 80% of Pb can be removed by using MOCR. The optimum reduction for Pb from WW was HFP, and HLS also showed reduction of Pb after treatment, the higher MOCR concentration, the higher removal of Pb from WW.

As shown in Figure 2, HFP has removed 99% of Cu, and HLS removed 100%, and this result was better than [30] results of Cu where MOCR was able to remove Cu up to 90%. The higher dosage of MOCR used the better Cu removal, and Cu was not detected after treatment with 2% and 3% of HFP.

Furthermore, Fe was fully removed by MOCR, and this result is in agreement with [4] [8] . Based on an investigation from [20] , removal of Fe by MOCR reached up to 92%.

Other than that, Cd was the other heavy metal that showed significant decreasing after the treatment. The ini-


Figure 2. Heavy metal removal from WW using MOCR. (a) hexane long storage batch (HLS); (b) hexane freshly prepared batch (HFP).

tial level of Cd was more than 1.58 mg/L when HFP and 1.718 mg/L when HLS. HFP has reduced the Cd to 0.034 mg/L by adding 1% MOCR, 0.03 mg/L using 2% MOCR, and 0.029 mg/L treated with 3% MOCR. HLS has reduced Cd up to 0.035 mg/L using 1% MOCR, 0.032 mg/L by adding 2% MOCR and 0.003 mg/L using 3% MOCR. Cd was removed up to 98 %. Storage period did not affect too much in the removal of Cd, nevertheless, the result of Cd removal was in contrast with [20] since the latter results showed 48% of Cd was removed which it might be due to the absence of pre-treatment step for MO seeds.

In this experiment, two factors affecting the efficiency of MOCR in heavy metal removal was examined. MOCR dose played the major role in the heavy metal removal; the higher MOCR dosage gives better reduction of heavy metal. According to Figure 1, and Figure 2, 1% of MOCR was enough to remove the heavy metal. Next, the storage duration was also examined, where the long storage batch (6 months) was compared with the freshly prepared batch. However, the long storage duration of MOCR was not affecting in heavy metal removal. Therefore, MOCR can be stored for long time up to 6 months without affecting the performance in water treatment.

3.4. Antibacterial Assay

MOCR was used to investigate the effect on bacteria presented in water. Table 3 shows the different bacteria count using MOCR for GR and WW water samples.

Before treatment with MOCR, there is high concentration of the bacteria present in the water sample for GR and WW. Both were unsafe for human consumption due to high amount of bacteria. Table 3 shows the initial concentration of bacteria in the GR and WW were 1.70 × 105 and 1.06 × 106 CFU/ml, respectively. Higher concentration of bacterial presence in the water sample might link to disease outbreak and it is harmful to environment. However, MOCR did not fully inhibit bacteria from water sample [2] [19] , but, it does help prevent the growth of microorganism. According to [8] , MOCR showed better antibacterial properties than chemical coagulant.

Based on Table 3, MOCR was successfully reduced the bacterial growth in water samples after treatment. The bacterial count in WW after treatment was 7.90 × 103 CFU/ml and almost same to the bacterial count after treatment of GR 8.00 × 103 CFU/ml. The storage duration is one of the factors affecting the antimicrobial effect of MOCR, HFP was slightly better than HLS in the removal of bacteria. However, the long storage duration do not deteriorate the MOCR since it showed the reduction of bacterial after the treatment as well.

It was reported by [19] that the antimicrobial activity is dependent on the concentration of MOCR, and the higher concentration used revealed better antimicrobial effect [8] [32] . The previous reports had emphasised that MOCR possess the antimicrobial function and reduce the number of bacterial colonies as reported by [13] [24] [33] [34] that active component from MO seed possess antibacterial properties and antipyretic.

Table 3. Bacteria count in GR & WW using MOCR.

1Hexane long storage batch; 2Hexane freshly prepared batch.

4. Conclusion

In general, MOCR was a good coagulant for water treatment. The extraction oil yield from MO seed was 34.77% by using hexane. It successfully reduced and prevented bacterial growth for WW and GR water samples. The turbidity was removed up to 97% after treatment, DO was significantly improved and no changing of pH but slightly alteration of certain water parameters such as conductivity, salinity and TDS as well. Increasing of COD and BOD cannot be avoided due to presence of organic matter in the MOCR. However, this did not bring the toxic effect and was not harmful to human health. Besides that, MOCR successfully removed the heavy metal from waste water, Fe was fully removed while Cu and Cd were successfully removed up to 98%, and the reduction of Pb was up to 82.17%. Overall, 1% of (MOCR) was enough to remove heavy metals from GR and WW.


The author would like to thank Research & Innovation Department/Universiti Malaysia Pahang/Malaysia, for financial support to publish this paper under grant #: RDU 140304. The author would like to thank the Faculty of Industrial Sciences and Technology/Universiti Malaysia Pahang/Malaysia for using their facilities to perform the experimental work.


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