2012. Vol.3, No.11, 959-965
Published Online November 2012 in SciRes (http://www.SciRP.org/journal/psych) http://dx.doi.org/10.4236/psych.2012.311144
Copyright © 2012 SciRes. 959
Hedonic Olfactory Perception in Depression: Relationship
between Self-Evaluation and Autonomic Response
Boriana Atanasova1, Philippe Gaillard1,2, Frédéric Minier1, Catherine Belzung1,
1INSERM U930 ERL 3106, University François Rabelais, Tours, France
2Department of Psychiatry, CHRU, Tours, France
Received July 9th, 2012; revised August 13th, 2012; accepted September 14th, 2012
This study aimed to improve the understanding of hedonic perception in depression using the olfactory
modality. We evaluated physiological (heart rate measures) and hedonic responses (subjective rating scale)
obtained from 30 unipolar depressed inpatients and 30 healthy controls. The stimuli were two odorants
with contrasting hedonic valence, vanillin (pleasant) and butyric acid (foul-smelling), presented at three
different concentrations and in nine binary mixtures. Compared to controls, the depressed subjects had
significantly increased heart rate response to olfactory stimuli, regardless of valence. These observations
were not related to the severity of depression. For both groups, a significant negative correlation was
found between the explicit hedonic rating and the implicit instantaneous heart rate measure. Together
these pilot findings suggest that unipolar depression is associated with stronger physiological reactions to
odorants and a negative bias when processing olfactory stimuli. Further studies are required to confirm
these observations in larger groups of depressed subjects.
Keywords: Olfaction; Depression; Hedonic Self-Evaluation; Heart Rate Response
Deficient positive affect, or anhedonia, and excessive nega-
tive affect (e.g. sadness, guilt) are the two cardinal symptoms of
major depressive disorder (MDD) as defined by the DSM-IV
(American Psychiatric Association, 1994). In a recent meta-
analysis, Bylsma et al. (2008) suggested that depressed indi-
viduals had reduced emotional reactivity (i.e. intensity of the
emotional response) to both positively and negatively valenced
stimuli, with a larger reduction for positive than for negative
stimuli as compared to healthy controls. However, the literature
on the emotional impairments in MDD is mainly centred on
processes supported by visual (pictures, films), verbal (words)
or auditory inputs (audiotapes of positive and negative social
interactions) (Bylsma et al., 2008). Among the many studies in
this field, only a few have explored the emotional and/or he-
donic response to olfactory stimuli in depressed individuals,
although olfactory processing has been shown to have close
links with emotional processing (Ehrlichman & Bastone, 1992;
Zatorre et al., 2000).
Olfactor y He donic Response and De pre ssion
Olfactory pleasantness is usually evaluated in depression us-
ing an explicit subjective self-rated assessments by different
types of scales (line bisection scale anchored on each end with
a descriptors, category scale, Likert scale, labeled magnitude
scale, self-assessment manikin scale…), with discrepant results.
Recent studie has demonstrated that the unpleasant stimulus of
butyric acid and the binary mixture of pleasant and unpleasant
odours (butyric acid and vanillin) are perceived as significantly
more unpleasant by depressed patients than by healthy controls.
No difference between groups was observed for the hedonic
evaluation of the pleasant stimulus of vanillin (Atanasova et al.,
2010). Earlier, Pause et al. (2001) found that only one odour
(citral) out of the ten studied was perceived as significantly
more pleasant by depressed patients than by controls. No dif-
ference between the hedonic scores of MDD patients and con-
trols was observed by three studies (Thomas et al., 2002;
Swiecicki et al., 2009; Clepce et al., 2010). Other studies have
found that MDD patients over-evaluate the pleasantness of
odorants compared to controls (Lombion-Pouthier et al., 2006;
Cumming et al., 2011).
All these results were obtained using self-reported subjective
evaluations of odour pleasantness. Overall, they suggest that
MDD patients’ response to olfactory stimuli is heightened or
remains unaffected. This is not in line with expected results
based on the observation that depressed people usually experi-
ence emotional cues less positively than controls and that their
ability to experience pleasantness is frequently impaired (an-
hedonia). The subjective hedonic rating requires the person to
give a cognitive estimation of his/her hedonic perception,
which may be impaired in depression. Thus, in order to de-
crease the risk of cognitive bias in odour hedonic evaluation, a
simultaneous objective measurement is needed.
Another possible explanation of the inconsistent findings of
the studies mentioned above is that some calculated the hedonic
scores by taking all odours irrespective of their hedonic valence,
and/or only pleasant stimuli were used (Lombion-Pouthier et al.,
2006; Clepce et al., 2010). Differences in methodological ap-
proaches, the inclusion of depression subtypes (e.g. unipolar,
bipolar MDD), and differences in medication could also ac-
count for the inconsistencies.
B. ATANASOVA ET AL.
Objective Ev aluation of the Ol f actory Hedonic
Indeed, the hedonic and/or emotional response to olfactory
stimuli can be evaluated also through implicit objective mea-
sures such as the behavioural analysis (e.g. analysis of facial
expressivity), the peripheral physiological reactivity evaluation
(e.g. skin resistance, skin blood flow, skin temperature, instan-
taneous heart rate, etc.) and the central reactivity evaluation (e.g.
event-related potentials, neuroimaging metrics). To our knowl-
edge, only one study has used an objective measure (event-
related potential analysis) to investigate the emotional response
to odour in depression (Pause et al., 2003). Furthermore, no
research has studied the physiological reactivity to odour in
depression, while it has been demonstrated that variations in
electrodermal (skin conductance, skin resistance), thermovas-
cular (skin blood flow) and cardiorespiratory (instantaneous
heart rate) responses could be modulated by odour pleasantness
(Alaoui-Ismaïli et al., 1997a, 1997b; Vernet-Maury et al., 1999;
Bensafi et al., 2002a). For instance, it has been shown that de-
creases in instantaneous HR variation are associated with
pleasant odours, while increases in HR variation are associ-
ated with unpleasant odours.
In an effort to remedy some of the shortcomings mentioned
above, two aspects were investigated in the present pilot study.
First, the olfactory response was studied using two different
evaluations: a subjective hedonic rating scale and an objective
measurement of instantaneous heart rate variation (HR). We
hypothesized that the depressed patients would perceive the
pleasant stimuli as less pleasant than controls and the un-
pleasant stimuli as more unpleasant than controls (hedonic as-
pect) using implicit objective method.
Secondly, the consistency between the explicit self-reported
hedonic response and the implicit instantaneous HR response
was investigated. We hypothesized that the relationship be-
tween both measures will be better for healthy controls com-
pared to patients.
Materials and Methods
We recruited 30 depressed inpatients with a current DSM-IV
diagnosis of major unipolar depression and 30 healthy controls
matched for age and gender. The severity of the depression was
evaluated using the Montgomery-Asberg Depression Rating
Scale (MADRS) (Montgomery and Asberg, 1979). Ten patients
had moderate depression (MADRS scores between 24 and 34)
and 20 had severe depression (MADRS > 34). The inpatients
were assessed 6.8 days on average (SD = 6.3) after admission
to hospital (Department of Psychiatry, Tours Hospital). The
DSM-IV axis I psychiatric co-morbidities were assessed by a
clinician using the French version of the Mini International
Neuropsychiatric Interview (MINI 5.0) (Duburcq et al., 1999).
The clinical interviews were carried out by a clinician. On the
day of the investigation, all patients were treated with antide-
pressants. Patients were excluded if they were not able to col-
laborate or have a verbal conversation due to the severity of
depression. The controls were healthy volunteers with no his-
tory of mental illness (Table 1). They were recruited by word-
of-mouth from families of staff members and from the local
All the participants had given prior written consent and were
Clinical description of the participants.
(n = 30)
(n = 30)
Female/male ratio 12/18 12/18
Age, years (SD) 34.6 (11.1) 33.4 (9.9)
Smoker, female/male ratio 5/13 4/5
MADRS, score (SD) 36.3 (6.3) 2.0 (2.1)
Duration of current episode.
months (SD) 6.7 (5.2) -
Number of previous episodes (SD)2.1 (1.8) -
MDD, current episode 30 -
Suicidal risk, last month 11 -
PTSD, last month 3 -
Other disorders* - -
MDD: major depressive disorder; PTSD: post-traumatic stress disorder; *(Hypo)-
Mania. Panic disorder. Obsessive compulsive disorder. Alcohol and cannabis
abuse (last 12 months). Psychotic disorder. Eating disorders (last 3 months).
Generalized anxiety disorder.
fully informed of the experimental protocol. All subjects were
informed that they were free to discontinue testing at any time.
They were instructed not to smoke for at least 30 - 40 min be-
fore the study. Participants were excluded if they had any prior
history of brain damage, neurological disorders, current sub-
stance abuse, any respiratory infection-induced olfactory loss,
odour allergy, current cold, any other medical condition liable
to impair olfactory ability (e.g. epilepsy, nasal polyps, etc.), or
anosmia to the odorants to be used.
The stimuli used in the present study were the same as those
used previously (Atanasova et al., 2010): vanillin (V) (pleasant
odour) and butyric acid (B) (unpleasant odour) supplied by
Fisher Scientific (Sigma, France). They were presented at three
different supra-threshold concentration levels (V1 = 60, V2 =
600, V3 = 6000 mg/l, and B1 = .03, B2 = .16, B3 = .8 mg/l) and
in nine possible combinations (binary mixtures). It has been
demonstrated that the quality descriptor of some odorants (their
respective hedonic valence) could change according to their
intensity (Moskowitz et al., 1976). For this reason, three dif-
ferent concentration levels of both odorants were used. How-
ever, during the experiment, no individual reported any change
of the odour quality descriptor of the three different concentra-
tion levels of the two odorants.
Three stimuli chosen at random (one pleasant: V3, one un-
pleasant: B1, and one binary mixture: B1V2) were presented
twice in order to study the test-retest reliability of the subjects’
responses. Only three stimuli were duplicated in order to avoid
olfactory fatigue and saturation. The odorants were diluted with
distilled water (the two compounds were soluble in this solvent
at the studied concentrations). The solutions were poured into
60 ml brown glass flasks (10 ml per flask) coded with a random,
Copyright © 2012 SciRes.
B. ATANASOVA ET AL.
The experimental session lasted approximately one hour. The
presentation order of the eighteen stimuli was balanced and was
identical for all the subjects in order to compare the results of
both groups. Prior to the measures, subjects were informed
verbally about the procedure. Firstly, the HR was recorded. The
subject was seated comfortably at a table in a quiet room. Each
flask containing the odour was opened and placed in front of
the subject. The subject was instructed to hold the flask ap-
proximately 1 - 2 cm from his/her nostrils with the electrode-
free hand and smell the odour for 1 to 2 seconds. This task had
to be carried out without any other movement or overt response.
The subject then placed the flask on the table and the experi-
menter closed the bottle and presented the next one. The inter-
trial interval was 2 min, which is the standard period used in
experiments recording autonomic parameters (Vernet-Maury et
al., 1999; Bensafi et al., 2002a). It also avoids olfactory adapta-
tion and fatigue. All these tasks and parameters were supervised
and monitored by the experimenter. Instantaneous HR variation
was evaluated using a monitoring electrode placed on the fore-
finger of the non-dominant hand. The measurements were car-
ried out using the Care ORD-049 monitor (CARE, Bobigny,
France). The time is displayed in seconds and the HR in beats
per minute (range: 40 to 199 bpm). HR was measured continu-
ously throughout the session. The HR score of each subject and
for each stimulus was calculated by subtracting the mean rate
for the 3 s preceding flask presentation from that for the 8 s
after odour stimulation as described previously (Bensafi et al.,
2002a; 2002b). After the HR variation measurements, the sub-
ject smelled each flask again and evaluated the pleasantness of
the odour using a linear scale labeled at each end (highly un-
pleasant/highly pleasant), resulting in a hedonic subjective
score ranging from 0 to 10 (cm). Prior to measurement session,
there was a short practice trial to familiarize the subjects with
The two-tailed paired Student test was used to study the test-
retest reliability of the subjects’ HR and hedonic responses.
HR measurements and hedonic self-evaluations of both
groups were computed separately with an analysis of variance
with 2 factors: stimulus (15 stimuli: B1, B2, B3, V1, V2, V3,
B1V1, B1V2, B1V3, B2V1, B2V2, B2V3, B3V1, B3V2, B3V3)
and group (depressed subjects and healthy controls) and one
their interaction (group × stimulus). As significant effects of
stimulus, group and group × stimulus interaction were found, a
two-by-two comparison between groups for each stimulus was
carried out using Tukey test.
Levene’s test for the homogeneity of variances revealed un-
equal variance for some variables. Parametric analyses have
nevertheless been applied in the present study, since the
ANOVA is relatively robust against violations of equal vari-
ances if the sample size is relatively high (more than 10) and
groups are of the same size (Box, 1954). Both conditions ap-
plied to our data.
Pearson’s correlations were used to assess the correlation
between self-reported hedonic responses and HR measurements
for each group. Pearson’s correlation coefficient was also cal-
culated to study the relationship between the severity of depres-
sion (MADRS score) and the HR scores on the one hand and
the severity of depression and the hedonic score on the other
hand of each pleasant and unpleasant stimulus.
The collected data were analysed using the SigmaStat 3.5
software. A probability value of p ≤ .05 was considered as sta-
Firstly, analysis of test-retest reliability of the subjects’ re-
sponses showed that both groups responded consistently to the
three studied stimuli (V3, B1 and B1V2), with both methods
(two-tailed paired Student test, df = 29, p > .05) (Table 2).
HR Measure m ent s
The HR response results revealed a significant effect of
stimulus (F(14,870) = 52.78, p < .001) and group (F(1,870) =
203.33, p < .001). With regard to the group × stimulus interac-
tion, the results showed a difference between the HR responses
of patients and controls according to the stimulus (F(14,870) =
2.91, p < .001). Comparing HR responses of patients and con-
trols (two-by-two comparison between groups for each stimulus)
revealed that for all odorant stimuli except one (B2), the mean
values of MDD patients were significantly higher than those of
healthy subjects (p < .05) (Table 3).
The analysis of variance indicated a significant effect of sti-
mulus (F(14,870) = 61.91, p < .001), group (F(1,870) = 53.13,
p < .001) and group × stimulus interaction (F(14,870) = 2.60,
p < .001). Comparison of the hedonic responses of patients and
controls showed that eight stimuli were perceived as signifi-
cantly less pleasant by MDD patients (two unpleasant stimuli
and six binary mixtures). No between-group difference was
observed for any pleasant stimuli and for three binary mixtures
(p > .05) (Tabl e 3).
The patients’ MADRS scores were not correlated with HR
responses for any pleasant stimuli (V1: r = .03, NS; V2: r = −.19,
NS; V3: r = .26, NS; n = 30), or any unpleasant stimuli (B1: r
= .03, NS; B2: r = −.04, NS; B3: r = −.06, NS; n = 30). There
was no relationship between the hedonic scores of each un-
mixed odorant and the depression scores (V1: r = −.16, NS; V2:
r = .22, NS; V3: r = .14, NS; B1: r = −.25, NS; B2: r = −.29, NS;
B3: r = −.15, NS; n = 30).
The relationship between hedonic responses and HR re-
sponses were studied for each group. The results revealed a sig-
nificant negative correlation between these two parameters
for patients (r = −0.86, p < .001, n = 15) and controls (r = −.79,
p < .001, n = 15) (Figure 1).
The general aim of the present study was to gain a better un-
derstanding of hedonic perception in depression using the ol-
factory modality. This involved two main objectives. The first
one was to compare the olfactory response of MDD patients
and healthy subjects obtained by subjective hedonic self-eva-
luation and objective HR measurements. The second was to
study the relationship between these two responses to olfactory
stimuli for both groups.
Copyright © 2012 SciRes. 961
B. ATANASOVA ET AL.
Copyright © 2012 SciRes.
Mean (SD) of heart rate variation score and hedonic response in depressed patients and healthy controls. R1: first repetition; R2: second repetition; V:
vanillin; B: butyric acid (p-value: two-tailed paired student test).
Depressed subjects Healthy subjects
Mean heart rate variation Mean hedonic score Mean heart rate variation Mean hedonic score
p p p p 1R 2R 1R 2R 1R 2R 1R 2R
(1.9) .43 1.7
(1.4) .67 5.8
(2.9) .12 2.6
(1.4) .19 B1
(1.3) .13 7.2
(2.1) .35 −2.5
(1.8) .14 7.1
(2.3) .67 V3
(1.8) .97 4.1
(2.3) .40 .3
(2.3) .49 5.2
(1.5) .80 B1V2
Mean (SD) heart rate variation and hedonic score to each olfactory stimulus in depressed patients and healthy controls. V: vanillin; B: butyric acid
(p-value: two-by-two comparison between groups for each stimulus using Tukey test).
Mean heart rate variation Mean hedonic score
Depressed subjects Healthy subjects p Depressed subjectsHealthy subjects p
B1 7.22 (1.75) 5.76 (2.29) .045 1.67 (1.07) 2.62 (1.56) .050
B2 6.90 (1.68) 7.27 (2.60) .610 1.26 (1.03) 2.48 (2.12) .012
B3 8.81 (1.91) 7.11 (3.00) .001 0.81 (.90) 1.42 (1.20) .205
V1 .84 (1.21) −1.30 (2.18) .020 5.50 (2.72) 4.91 (2.21) .225
V2 .85 (1.64) −2.53 (1.97) <.001 7.38 (1.90) 7.09 (1.94) .779
V3 1.35 (2.07) −2.50 (2.24) <.001 7.21 (2.18) 7.52 (2.02) .805
B1V1 2.82 (2.73) .54 (2.03) .002 3.64 (2.57) 4.54 (1.34) .065
B1V2 2.87 (2.18) .33 (2.40) <.001 4.10 (2.57) 5.20 (1.08) .024
B1V3 2.02 (1.57) −.08 (2.35) .004 5.12 (2.00) 5.79 (2.02) .166
B2V1 3.37 (2.56) .84 (1.83) <.001 2.47 (1.89) 4.10 (1.35) <.001
B2V2 3.45 (3.01) .79 (2.59) <.001 2.35 (1.92) 4.46 (1.48) <.001
B2V3 3.40 (2.55) .35 (1.81) <.001 3.54 (2.38) 5.89 (2.16) <.001
B3V1 3.70 (2.10) .31 (2.07) <.001 1.52 (1.80) 2.75 (1.90) .011
B3V2 4.13 (2.31) .50 (1.81) <.001 1.72 (1.77) 2.80 (1.51) .026
B3V3 4.30 (2.38) .69 (2.18) <.001 2.51 (2.44) 2.95 (1.58) .370
Heartrate variation (beats/min)
Hedonicscore (cm)Hedonicscore (cm)
r = -.86
p < .001
r = -.79
p < .001
(a) Depressed subjects (b) Healthy subjects
Link between heart rate variation and hedonic self-evaluation of olfactory stimuli in both groups of subjects. Each point corresponds to an
olfactory stimulus (V: vanillin; B: butyric acid).
B. ATANASOVA ET AL.
For the first objective, the results of the hedonic self-eva-
luation showed a significant difference between the two groups
for two unpleasant unmixed stimuli (butyric acid) and for most
of the binary mixtures (butyric acid/vanillin), which were per-
ceived as significantly more unpleasant by the patients. No
difference between groups was observed for pleasant stimuli.
These last results for pleasant stimuli are in accordance with the
literature (Pause et al., 2001; Thomas et al., 2002; Swiecicki et
al., 2009; Clepce et al., 2010). However, for the cited above
studies the calculation method of the subjects’ pleasantness
considers all the odors irrespective of the hedonic valence of
With regard to the objective HR measurement, the results
revealed an increase in the HR responses of depressed patients
compared to controls for almost all stimuli: two unpleasant
olfactory stimuli, all pleasant olfactory stimuli, and all binary
mixtures. Our observations in patients’ group are in line with
the results of Siegle et al. (2001) with subjects with the same
diagnosis of major unipolar depression. More precisely, using
pupillary dilation measures in the valence identification task,
the authors showed that depressed patients displayed greater
sustained processing (pupil dilation) in response to positive,
negative and neutral stimuli (words) than non-depressed sub-
jects. A similar phenomenon as in the present study was also
observed in MDD individuals by Kaviani et al. (2004). The
authors found that MDD subjects demonstrated anxiety levels
(self-reported measure) associated with increased reactivity for
both positive and negative valenced film clips. These observa-
tions suggest that the autonomic nervous system could be a
reliable mirror of the emotional stimuli response in major uni-
polar depression. However, future studies measuring several
other psychophysiological parameters of emotional stimulus
response are needed to confirm this suggestion.
Previously, two research teams demonstrated that an increase
in HR variation typically accompanies an unpleasant odorant
inhaling experience (Alaoui-Ismaïli et al., 1997b; Bensafi et al.,
2002a, 2002b). Our findings suggest that the implicit objective
measures support the same negative “bias” as the subjective
explicit responses. We could suppose that the presence of an
olfactory cognitive bias observed in the depressed subjects
could be related to the selective processing of negative infor-
mation shown by these subjects, who are known for making
dysfunctional attributions, and for engaging in more negative
For all stimuli except the pleasant ones, the HR responses of
the subjects in our study were consistent with their subjective
ratings. One possible explanation of these divergent results is
that the explicit hedonic rating may involve a higher decisional
cognitive process, which may be impaired in depression. The
objective perception of the positive odour as being less pleasant
could also be due to anhedonia, a well-known symptom of de-
pression which involves a gradual loss of the ability to experi-
ence physical and emotional pleasure. The neural representa-
tions of anhedonia and the hedonic evaluation of odours over-
lap significantly within a network consisting of the prefrontal
and orbitofrontal cortex, the subcortical structures of the reward
system and the limbic system, including the amygdala (Zatorre
et al., 2000; Anderson et al., 2003; Keedwell et al., 2005; Gor-
wood, 2008). Abnormal activation in the amygdala and the
orbitofrontal region has also been reported in depression (Dre-
vets et al., 1992; Lesser et al., 1994). We can assume that the
MDD patients’ decreased hedonic response to the pleasant
stimuli is connected to the theory of “positive attenuation”, in
other words, reduced reactivity to positive stimuli. Further re-
search is needed to assess anhedonia in depressive patients (e.g.
by using a self-rating scale; Chapman et al., 1976; Fawcett et al.,
1983) in order to validate the hypothesis of a relationship be-
tween anhedonia and the decreased hedonic perception of
pleasant stimuli revealed by the HR measures among depressed
With regard to the second objective, our results indicated a
significant relationship between the subjective explicit hedonic
response and the objective HR response for both groups.
Moreover, the hedonic and the HR variation scores for pleasant
and unpleasant stimuli did not vary with the severity of depres-
sion. These observations are in line with recent results obtained
by Sloan and Sandt (2010) in individuals with depression
symptoms using pictures as emotional stimuli. The authors
found no relationship between more severe symptoms of de-
pression and blunted or heightened responses, as indicated by
autonomic reactivity and self-report measures. Likewise, no
correlation was found between severity of depression and the
pleasantness rating of olfactory (Atanasova et al., 2010) and
gustatory stimuli (Swiecicki et al., 2009).
No field data are available concerning any link between he-
donic rating and HR variation responses in depression. Fur-
thermore, the relationship between the subjective and objective
responses of patients has previously been studied in the visual
sensory modality; the objective measure involved analysing
videotapes of the subjects’ facial expressions recorded while
they watched slides evoking different emotional responses
(Berenbaum & Oltmanns, 1992; Sloan et al., 1997). No sig-
nificant relationship between the two types of measure was
found for MDD patients in either of these studies. However,
Sloan et al. (1997) pointed out that the slides depicting positive
emotion may not have been sufficiently evocative because they
did not elicit strong facial expressions in either depressive or
Some limitations of the present study merit discussion. First,
only two odorants were used. Further research is needed to
confirm our results and to extend the study of emotional re-
sponses to olfactory stimuli using several odours with con-
trasting hedonic valence.
Another limitation concerns smoking. The number of smok-
ers is twice as high in patients as in controls. Smoking may
reduce olfactory performance depending on the duration and
dose (Frye et al., 1990). All the smokers in our study refrained
from smoking for at least 30 - 40 min before the experiment, so
there was no acute effect of smoking on the olfactory percep-
tion. However, a more chronic effect cannot be ruled out as we
have no information about our subjects’ smoking habits. Future
research is required to investigate the potential effects of
smoking on olfactory perception and hedonic processing in
depression. Thirdly, we cannot rule out the possibility that our
results were affected by specific medication. Nevertheless,
previous studies on olfaction failed to find any effects of stan-
dard psychotropic medication (Martzke et al., 1997; Pause et al.,
2001). At last, we can also hypothesize that our results could be
due to the changes in of autonomic regulation of the heart in
depression, such as activation of the sympathetic nervous sys-
tem. Knowing that the results in this field were often inconclu-
sive (Lehofer et al., 1997), this aspect must be check on in fur-
ther studies aimed to replicate our observations.
Copyright © 2012 SciRes. 963
B. ATANASOVA ET AL.
The present study has added knowledge concerning the ol-
factory hedonic perception in MDD. Using implicit instantane-
ous HR measures, findings revealed that depressed individuals
were characterized by increased negative perception of olfac-
tory stimuli whatever their hedonic valence. The results also
suggest that there is a positive attenuation effect specific to
pleasant olfactory stimuli. The results raise the question of
whether the negative bias seen in depressed patients is a direct
result of the clinical condition, or reflects permanent trait ab-
normality. To answer this question, two complementary mea-
surements are required: one before psychiatric treatment, and
one during a follow-up investigation a few months after remis-
sion. A close relationship between the explicit subjective he-
donic response and the implicit instantaneous HR measure was
also observed for both groups, suggesting that these two meth-
ods could be used for olfactory hedonic evaluation.
Alaoui-Ismaïli, O., Robin, O., Rada, H., Dittmar, A., & Vernet-Maury,
E. (1997a). Basic emotions evoked by odorants: comparison between
autonomic responses and self-evaluation. Physiology & Behavior, 62,
Alaoui-Ismaïli, O., Vernet-Maury, E., Dittmar, A., Delhomme, G., &
Chanel, J. (1997b). Odor hedonics: Connection with emotional re-
sponse estimated by autonomic parameters. Chemical Senses, 22,
American Psychiatric Association (1994). Diagnostic and statistical
manual of mental disorder (4th ed.). Washington DC: American
Anderson, A. K., Christoff, K., Stappen, I., Panitz, D., Ghahremani, D.
G., Glover, G., Gabrieli, J. D. E., & Sobel, N. (2003). Dissociated
neural representations of intensity and valence in human olfaction.
Nature Neuroscience, 6, 196-202. doi:10.1038/nn1001
Atanasova, B., El-Hage, W., Chabanet, C., Gaillard, P., Belzung, C., &
Camus, V. (2010). Olfactory anhedonia and negative olfactory alli-
esthesia in depressed patients. Psychiatry Research, 176, 190-196.
Bensafi, M., Rouby, C., Farget, V., Bertrand, B., Vigouroux, M., &
Holley, A. (2002a). Influence of affective and cognitive judgments
on autonomic parameters during inhalation of pleasant and un-
pleasant odors in humans. Neuroscience Letters, 319, 162-166.
Bensafi, M., Rouby, C., Farget, V., Bertrand, B., Vigouroux, M., &
Holley, A. (2002b). Autonomic nervous system responses to odours:
The role of pleasantness and arousal. Chemical Senses, 27, 703-709.
Bensafi, M., Rouby, C., Farget, V., Vigouroux, M., & Holley, A.
(2002). Asymmetry of pleasant vs unpleasant odor processing during
affective judgment in humans. Neuroscience Letters, 328, 309-313.
Berenbaum, H., & Oltmanns, T. F. (1992). Emotional experience and
expression in schizophrenia and depression. Journal of Abnormal
Psychology, 101, 37-44. doi:10.1037/0021-843X.101.1.37
Bylsma, L. M., Morris, B. H., & Rottenberg, J. (2008). A meta-analysis
of emotional reactivity in major depressive disorder. Clinical Psy-
chology Review, 28, 676-691. doi:10.1016/j.cpr.2007.10.001
Chapman, L. J., Chapman, J. P., & Raulin, M. L. (1976). Scales for
physical and social anhedonia. Journal of Abnormal Psychology, 85,
Clepce, M., Gossler, A., Reich, K., Kornhuber, J., & Thuerauf, N.
(2010). The relation between depression, anhedonia and olfactory
hedonic estimates—A pilot study in major depression. Neuroscience
Letters, 471, 139-143. doi:10.1016/j.neulet.2010.01.027
Cumming, A. G., Matthews, N. L., & Park, S. (2011). Olfactory identi-
fication and preference in bipolar disorder and schizophrenia. Euro-
pean Archives of Psychiatry and Clinical Neuroscience, 261, 251-
Drevets, W. C., Videen, T. O., Price, J. L., Preskorn, S. H., Carmichael,
S. T., & Raichle, M. E. (1992). A functional anatomical study of
unipolar depression. The Journal of Neuroscience: The Official
Journal of the Society f o r N e u r o s c i e nc e , 12, 3628-3641.
Duburcq, A., Blin, P., Charpak, Y., Blachier, C., Allicar, M. P., Bou-
hassira, M., Hergueta, T., & Lecrubier, Y. (1999). Use of a structured
diagnostic interview to identify depressive episodes in an epidemic-
ologic study: A posteriori internal validation. Revue d’épidémiologie
et de santé publique, 47, 455-463.
Ehrlichman, H., & Bastone, L. (1992). Olfaction and emotion. In M.
Serby, & K. L. Chobor (Eds.), Science of olfaction (pp. 410-438).
Springer-Verlag, New York. doi:10.1007/978-1-4612-2836-3_15
Fawcett, J., Clark, D. C., Scheftner, W. A., & Gibbons R. D. (1983).
Assessing anhedonia in psychiatric patients. Archives of General
Psychiatry, 40, 79-84. doi:10.1001/archpsyc.1983.01790010081010
Frye, R. E., Schwartz, B. S., & Doty, R. L. (1990). Dose-related effects
of cigarette smoking on olfactory function. The Journal of the
American Medical Association, 263, 1233-1236.
Gorwood, P. (2008). Neurobiological mechanisms of anhedonia. Dia-
logues in Clinical Neurosc ie n c e , 10, 291-299.
Kaviani, H., Gray, J., Checkley, S., Raven, P., Wilson, G., & Kumari,
V. (2004). Affective modulation of the startle response in depression:
influence of the severity of depression, anhedonia, and anxiety.
Journal of Affective Disor d ers, 83, 21-31.
Keedwell, P. A., Andrew, C., Williams, S. C. R., Brammer, M. J., &
Phillips, M. L. (2005). The neural correlates of anhedonia in major
depressive disorder. Biological Psychiatry, 58, 843-853.
Lehofer, M., Moser, M., Hoehn-Saric, R., McLeod, D., Liebmann, P.,
Drnovsek, B., Egner, S., Hildebrandt, G., & Zapotoczky H. G. (1997).
Major depression and cardiac autonomic control. Biological Psy-
chiatry, 42, 914-919. doi:10.1016/S0006-3223(96)00494-5
Lesser, I. M., Mena, I., Boone, K. B., Miller, B. L., Mehringer, C. M.,
& Wohl, M. (1994). Reduction of cerebral blood flow in older de-
pressed patients. Archives of General Psychiatry, 51, 677-686.
Lombion-Pouthier, S., Vandel, P., Nezelof, S., Haffen, E., & Millot, J.
L. (2006). Odor perception in patients with mood disorders. Journal
of Affective Disorders, 90, 187-191. doi:10.1016/j.jad.2005.11.012
Martzke, J. S., Kopala, L. C., & Good, K. P. (1997). Olfactory dysfunc-
tion in neuropsychiatric disorders: Review and methodological con-
siderations. Biological Psychiatry, 42, 721-732.
Montgomery, S. A., & Asberg, M. (1979). A new depression scale
designed to be sensitive to change. The British Journal of Psychiatry:
The Journal of Mental Science, 1 3 4 , 382-389.
Moskowitz, H. R., Dravnieks, A., & Klarman, L. A. (1976). Odor in-
tensity and pleasantness for a diverse set of odorants. Perception &
Psychophysics, 19, 122-128. doi:10.3758/BF03204173
Pause, B. M., Raack, N., Sojka, B., Göder, R., Aldenhoff, J. B., &
Ferstl, R. (2003). Convergent and divergent effects of odors and
emotions in depression. Psychophysiology, 40, 209-225.
Pause, B. M., Miranda, A., Göder, R., Aldenhoff, J. B., & Ferstl, R.
(2001). Reduced olfactory performance in patients with major de-
pression. Journal o f Psychiatric Research, 35, 271-277.
Siegle, G., Granholm, E., Ingram, R. E., & Matt, G. E. (2001). Pupil-
lary and reaction time measures of sustained processing of negative
information in depression. Biological Psychiatry, 49, 624-636.
Sloan, D. M., Strauss, M. E., Quirk, S. W., & Sajatovic, M. (1997).
Subjective and expressive emotional responses in depression. Jour-
nal of Affective Disorders, 46, 135-141.
Sloan, D. M., & Sandt, A. R. (2010). Depressed mood and emotional
Copyright © 2012 SciRes.
B. ATANASOVA ET AL.
Copyright © 2012 SciRes. 965
responding. Biol og ica l Psychology, 84, 368-374.
Soussignan, R., Schaal, B., Marlier, L., & Jiang, T. (1997). Facial and
autonomic responses to biological and artificial olfactory stimuli in
human neonates: Re-examining early hedonic discrimination of
odors. Physiology & Behavior, 62, 745-758.
Swiecicki, L., Zatorski, P., Bzinkowska, D., Sienkiewicz-Jarosz, H.,
Szyndler, J., & Scinska, A. (2009). Gustatory and olfactory function
in patients with unipolar and bipolar depression. Progress in Neuro-
Psychopharmacology and Bio logi cal Psychiatry, 33, 827-834.
Thomas, H. J., Fries, W., & Distel, H. (2002). Evaluation of olfactory
stimuli by depressed patients. Der Nervenarzt, 73, 71-77.
Vernet-Maury, E., Alaoui-Ismaïli, O., Dittmar, A., Delhomme, G., &
Chanel, J. (1999). Basic emotions induced by odorants: A new ap-
proach based on autonomic pattern results. Journal of the Autonomic
Nervous System, 75, 176-183. doi:10.1016/S0165-1838(98)00168-4
Zatorre, R. J., Jones-Gotman, M., & Rouby, C. (2000). Neural me-
chanisms involved in odor pleasantness and intensity judgments.
Neuroreport, 11, 2711-2716.