d? And how is it regulated? Is it carried out by a single form of GAPDH or it results from the induction of different isoforms?

This could help to develop a gene therapy with antisense oligodeoxynucleotides directed against the mRNA isoform (s) in cancer cases involving overexpression of GAPDH.

4. CONCLUSIONS

Our study has shown that in the three organs studied: Cervix, Breast and Prostate, GAPDH showed an overexpression in malignant tumor tissues. It has been shown that labeling was essentially nuclear in malignant lesions.

These results suggest GAPDH involvement in cancer process and draw attention to a probable new nuclear role. In addition to other functions already described such as its implication in glycolysis, apoptosis or oxidative stress, GAPDH may be implicated in DNA replication or repair.

Although GAPDH seems to be involved in malignant tumor process, there is no certainty about its specific role in the studied pathologies. Therefore it would be very interesting to evaluate the expression of the gene encoding this enzyme.

Moreover, it seems important to demonstrate whether the new role of GAPDH remains specific to a single form, or it is related to different isoforms of the enzyme, which can help to develop a gene therapy with antisense oligodeoxynucleotides directed against isoform(s) mRNA(s) in cases of cancer involving overexpression of GAPDH.

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