Journal of Cancer Therapy
 Vol.4 No.2(2013), Article ID:29920,6 pages DOI:10.4236/jct.2013.42074

Phase II Study of Irinotecan plus S-1 in Treatment of Advanced Gastric Cancer*

Hideki Bou1#, Akira Tokunaga1, Hideyuki Suzuki1, Nobuo Murata2, Yasuyuki Sugiyama2, Naoto Fukuda2, Masahiro Ishimaru3, Hiroyuki Suzuki3

1Institute of Gastroenterology, Nippon Medical School Musashi Kosugi Hospital, Kawasaki, Japan; 2Department of Surgery, University Hospital of Mizonokuchi, Teikyo University School of Medicine, Kawasaki, Japan; 3Department of Surgery, Kanto Rosai Hospital, Kawasaki, Japan.

Email: #bou@nms.ac.jp

Copyright © 2013 Hideki Bou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received December 28th, 2012; revised January 31st, 2013; accepted February 8th, 2013

Keywords: Advanced Gastric Cancer; Systemic Chemotherapy; Irinotecan; S-1

ABSTRACT

Objective: The efficacy and safety of irinotecan hydrochloride (CPT-11) plus oral fluoropyrimidine S-1 combination therapy in patients with previously untreated advanced gastric cancer was evaluated. Methods: The regimen comprised CPT-11 plus S-1: CPT-11, 60 mg/m2 (days 1, 15); S-1, 40 - 60 mg/body twice daily (days 1 - 21) followed by a 1-week rest, every 4 weeks. Primary endpoint was response rate. Secondary endpoints were tumor control rate, adverse events, relative dose intensity, and overall survival. Results: Twenty-five patients were enrolled; median age was 66 years. Response rate was 40% (95% confidence interval, 21.1% - 61.3%; complete response in 1; partial response in 9). Tumor control rate was 56.0%, median survival time was 436 days and relative dose intensities were 0.83 for CPT-11 and 0.85 for S-1. Incidence of grade 3 or greater neutropenia, anemia and diarrhea was 16%, 12%, and 12%, respectively. Conclusion: The present results indicate that CPT-11 plus S-1 offers lower treatment-related toxicity than regimens including cisplatin and is effective in patients with advanced gastric cancer.

1. Introduction

Although chemotherapy has been reported to significantly prolong survival compared with best supportive care in the treatment of advanced or recurrent gastric cancer [1-3], the standard regimen remains to be established. In Japan, 5-fluorouracil (5-FU) is the most common choice in the treatment of advanced or recurrent gastric cancer. In 1992, the Japan Clinical Oncology Group (JCOG) conducted a study to compare monotherapy with 5-FU with 5-FU+cisplatin (CDDP) (FP) or UFT+mitomycin C (UFTM). Neither FP nor UFTM produced better therapeutic results than monotherapy with 5-FU [4]. This suggests limitations in exploring standard treatment based mainly on 5-FU, and therefore the advent of a new antitumor agent is awaited.

Irinotecan hydrochloride (CPT-11) is an antitumor agent developed in Japan. Its mechanism of action involves the inhibition of topoisomerase I (Topo I) [5], and it has been reported to be effective against advanced gastrointestinal cancer [6]. The response rate (RR) to monotherapy with CPT-11 was 18.4% in eligible patients with advanced or recurrent gastric cancer. Approximately half of the patients developed reversible leucopenia. Clinical studies of combination therapy with CPT-11 and other chemotherapeutic agents have also shown promising results [7]. The RR after combination chemotherapy with CPT-11 and CDDP has been reported to be between 42% and 59%, and median survival time (MST) to be 365 days [8-10]. However, combination therapy with CDDP is highly toxic and has been reported to involve a high incidence of adverse events. Yano et al. [11] stated that CDDP should only be used by specialists who were familiar with treatment with antitumor agents.

An oral 5-FU derivative developed in Japan, S-1, is now available in capsule form together with tegafur (a prodrug of 5-FU), gimeracil (5-Chloro-2, 4-dihydroxypyridine) and otastat potassium. This combination is believed to enhance the antitumor effect of 5-FU by increasing its concentration and reducing gastrointestinal toxicity [12,13]. As the response rate to monotherapy with this drug is as high as 49% to 53.6% in patients with stomach cancer, it is now becoming the de facto standard treatment for advanced or recurrent gastric cancer [14,15]. The effects and therapeutic results of chemotherapy based on 5-FU, including S-1, are related to expression of thymidylate synthase (TS) [16]. Ichikawa et al. [17] showed that whereas monotherapy with S-1 produced a poor therapeutic effect in patients with high TS expression, S-1 in combination with CPT-11 was effective. Therefore, clinical studies of combination therapy with CPT- 11 and S-1 have been conducted in patients with advanced gastric cancer [18-21].

A number of studies have investigated the optimum dosage for combination therapy with CPT-11 and S-1. Therefore, this multicenter phase II focused on investigating the efficacy and safety of CPT-11 plus S-1 combination therapy in patients with advanced gastric cancer.

2. Patients and Methods

2.1. Eligibility Criteria

Patients with unresectable advanced or recurrent gastric cancer were enrolled. The patients were required to satisfy the following eligibility criteria: histologically confirmed diagnosis of gastric cancer with measurable lesion; age of 20 to 75 years; Eastern Cooperative Oncology Group (ECOG) performance status of 0-1; no prior chemotherapy regimen including CPT-11 or S-1; leukocyte count of 4000 - 12,000/mm3, platelet count of >100,000 mm3, and hemoglobin >9.5 g/dl; aspartate aminotransferase and alanine aminotransferase <100 IU/l and total bilirubin <1.5 mg/dl; creatinine level of <1.2 mg/dl; electrocardiogram findings, normal. Written informed consent was required from all patients prior to participation in the study. Patients with any of the following conditions were excluded: severe, co-existing medical illness (including intestinal paresis or ileus, interstitial pneumonia, pulmonary fibrosis, and poorly controlled diabetes mellitus); severe psychiatric disturbances. This study was conducted with the approval of the ethics committee of each participating institution.

2.2. Treatment Schedule

Irinotecan was initially given at a dose of 60 mg/m2 over 90 min by intravenous infusion on days 1 and 15 (Figure 1). S-1 was administered orally according to body surface area twice daily (40 - 60 mg/body twice daily, Table 1) for 3 consecutive weeks. This treatment was repeated every 4 weeks until disease progression, refusal by the patient, or unacceptable adverse reaction.

Prior to chemotherapy, patients received antiemetics comprising 5-HT3 receptor antagonists and steroids. Episodes of diarrhea were treated with loperamide hydrochloride as required.

The following dose adjustments were permitted: if grade 4 hematologic or grade 2 non-hematologic toxicity occurred, the dose of CPT-11 was reduced to 40 mg/m2 and treatment continued; if serum creatinine level reached 1.2 to 1.5 mg/dl, the dose of S-1 was reduced by 16.7% - 32.5% (Table 2) and treatment continued.

Evaluation

The primary endpoint was RR; the secondary endpoints were tumor control rate, adverse events, relative dose intensity, and overall survival.

Tumor response was evaluated based on change in the size of measurable lesions and assessment of evaluable lesions. Measurable lesions and evaluable lesions were defined and efficacy evaluated in accordance with the Japanese Criteria for Evaluating the Efficacy of Chemotherapy and Radiation Therapy in the Treatment of Gastric Cancer [22]. In brief, complete response (CR) was

Figure 1. Treatment schedule.

Table 1. Criterial dose of S-1.

Table 2. Criteria for dose reduction.

*Excluding abnormal laboratory findings, nausea, appetite loss and general fatigue.

defined as the disappearance of all evidence of the tumor for at least 4 weeks. Partial response (PR) was defined as a 50% or greater reduction in the sum of the products of the perpendicular diameters of all measurable lesions for at least 4 weeks without any evidence of new lesions or the progression of any existing lesions. Stable disease (SD) was defined as a less than 50% reduction or less than 25% increase in the sum of the products of the perpendicular diameters of all lesions for at least 4 weeks without any evidence of new lesions or the progression of any existing lesions. Progressive disease was defined as a >25% increase in one or more lesions or the appearance of new lesions. Tumor measurements were performed every 4 weeks using computed tomography, plain chest X-ray films, upper gastrointestinal endoscopy, and ultrasonography. Primary tumors were classified as follows based on X-ray and endoscopic findings: measureable, not measurable but evaluable, or diffused infiltration.

The NCI-CTC ver. 2.0 was used to evaluate adverse events. An independent committee reviewed the eligibility and suitability for assessment of the subjects and response to treatment.

3. Results

3.1. Patient Characteristics

Twenty-five patients (23 men, 2 women) were enrolled in this study between November 2004 and August 2008. The clinical characteristics of the patients are shown in Table 3. All patients met the entry criteria and were included in the analysis. Three patients (3 men) had received prior chemotherapy (doxifluridine (5’-DFUR) was used in 2 and UFT+Paclitaxel (PTX) in 1). The median age of the patients was 66 years (range, 47 - 78 years). Histologically, 11 patients had poorly differentiated adenocarcinoma, 10 had tubular adenocarcinoma, 2 had signet-ring cell carcinoma, 1 had papillary adenocarcinoma, and 1 was unknown. Performance status was 0 in 23 patients and 1 in 2 patients.

3.2. Tumor Response and Survival

Among 25 patients with evaluable lesions, CR was observed in 1 patient and PR in 9. The overall RR was 40% (95% confidence interval, 21.1% - 61.3%) in Table 4. The tumor control rate (SD or better response) was 56.0% (14/25). The RR according to site was 41.7% (5/12) for abdominal lymph node metastases and 27.3% (3/11) for liver metastases. Median survival time was 436 days (95% confidence interval, 61 - 212 days) in the 25 patients (Figure 2). Median number of treatment cycles given was 4 (range, 0.5 - 19). Patients were taken out of the study due to worsening of primary disease (10 patients), adverse events (8 patients), refusal of treatment (1 patient), operation (2 patients), or other (4 patients). Relative dose intensities were 0.83 for CPT-11 and 0.85 for S-1.

Table 3. Patient characteristics.

Table 4. Response rate (RR).

*NE: not evaluable. CR: complete response; PR: partial response; SD: stable disease; PD: progressive disease.

3.3. Safety

Adverse events among the 25 patients are shown in Table 5. The main grade 3/4 hematological toxicities observed were leucopenia (12%), neutropenia (16%), and anemia (12%). The grade 3/4 non-hematological toxicity was diarrhea (12%). Combination therapy was tolerable and mild. No treatment-related deaths occurred.

4. Discussion

Mainstream chemotherapy for advanced, recurrent gastric cancer involves multiple agents, which provide a high anti-tumor effect and survival benefit. In the US, 5-FU + ADR + MTX (FAMTX) used to be the standard therapy in such cases. In recent years, however, epirubicin + CDDP + 5-FU (ECF) therapy has become a standard option in such patients following the publication of

Figure 2. Overall survival.

Table 5. Toxicity.

two studies: the US study V325, in which 5-FU/CDDP (CF) was compared with CF plus docetaxel (DCF), and a European study, in which FAMTX was compared with ECF. These triplet regimens can exert a high anti-tumor effect, but may also be accompanied by severe toxicity.

In Japan, continuous intravenous infusion of 5-FU has been used as a reference arm in trials, and various doublet regimens have been studied. Recent phase III studies such as JCOG9912 [23] and SPIRITS [24] have changed the use of each of these agents. The JCOG9912 study demonstrated the non-inferiority of S-1 over continuous 5-FU infusion, but not the superiority of CPT-11/CDDP therapy. CPT-11/CDDP was shown to be superior to continuous 5-FU infusion, with a significant improvement in progression-free survival (4.8 months vs. 2.9 months, p < 0.001), but no significant difference in MST (12.3 months vs. 10.8 months, p = 0.055). Consequently, in light of its milder toxic effects, S-1 became a key drug in Japan. Around the same time, the SPIRITS study evaluated the efficacy of CDDP added to S-1. Progressionfree survival was 4.0 months vs. 6.0 months (p < 0.0001), demonstrating the efficacy of this add-on therapy. Thus, Japanese guidelines currently recommend S-1/CDDP for first-line treatment for advanced, recurrent gastric cancer. Meanwhile, the phase III GC0301 study [25] investigated the efficacy of CPT-11 when added to S-1. A significant difference was observed in RR (26.9% vs. 41.5%, p = 0.035), but not in survival (10.5 months vs. 12.8 months, p = 0.233), which was the primary endpoint of that study. CPT-11/S-1 therapy is not presently recommended as first-line therapy. However, CPT-11 remains an effective second-line agent.

At the time of planning the present study, both the JCOG9912 and SPIRITS studies were still in progress, and the most recent JCOG9205 report did not show an additional effect of CDDP. Therefore, we decided to investigate CPT-11/S-1 therapy, taking into account that hydration requires short-term hospitalization. We designed a regimen aimed at lower toxicity and higher treatment continuity based on the CPT-11/S-1 results reported in the OGSG002 trial [26]. We left the dose intensity unaltered, but reduced the amount of each dose of CPT- 11, which is known to exert severe toxic effects, even when administered at a low dose. The results suggest a new treatment option.

The results of the present study showed a good survival outcome (436 days), although the overall RR (40%) was relatively low compared with that in other phase II studies. Interestingly, the treatment effect varied depending on histological type, with RR (Table 4), MST (959 days, 279 days), and time-to-treatment failure (248 days, 102 days) being better in patients with differentiated carcinoma. These results were comparable with those in the OGSG002 study (overall RR, 47.8%; intestinal RR, 60.0%; diffuse RR, 38.5%); and in a retrospective study [10] of CPT-11/CDDP therapy, differentiated carcinoma was shown to have a significantly greater survival time (differentiated, 472 days vs. undifferentiated, 291 days, p = 0.0115). Thymidylate synthase activity may explain this. Ichikawa et al. confirmed higher TS activity in patients with differentiated gastric cancer, as well as a lower effect of S-1 monotherapy. They also reported that the addition of CPT-11 down-regulates TS activity, so that a therapeutic effect could be expected, even if TS activity was high. Thus, CPT-11 may exert a higher treatment effect, particularly in differentiated gastric cancer. This is consistent with the findings of Katsube et al. [27].

Sub-analyses of the SPIRITS and JCOG9912 studies, both of which involved CDDP as a drug of interest, showed superior outcomes in diffuse-type gastric cancer, suggesting that the treatment effect of CDDP is markedly higher in this type. The GC0301 study also indicated that combination therapy with CPT-11 may reduce risks in diffuse-type gastric cancer. However, Japanese phase III studies have not taken into account the presence or absence of a target (measurable) lesion, and the patient characteristics have been substantially different from ours, with eligible patients showing an RR of approximately 60% compared with the overall patient population (SPIRIT and GC0301 studies). Taking this into account, we cannot exclude the possibility that other factors may be involved. Furthermore, the JCOG9912 study showed that CPT-11/CDDP therapy yielded a significantly higher survival benefit than 5-FU in patients with target lesions, while a statistically significant improvement in survival time was observed for S-1 monotherapy in those with nontarget lesions. In addition, combination with CDDP significantly improved overall survival in patients with peritoneal dissemination and/or patients without target lesions in the SPIRITS trial. Therefore, CPT-11 is considered to have a high therapeutic effect, even for target lesions, and it may be possible to select treatment based on pathological condition in patients whose gastric cancers have hetero-type tumor characteristics.

The results of the present study, which have been confirmed to be reproducible, suggest that CPT-11/S-1 therapy has lower treatment-related toxicity than regimens including CDDP and a greater effect in differentiated carcinoma. Although sufficient efficacy was not established in phase III studies in which first-line regimens were investigated, this CPT-11-containing treatment strategy may well exert a therapeutic effect in patients with differentiated-type tumors and/or target lesions, warranting further studies focusing on such patient populations.

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NOTES

*Conflict of Interest Statement: None of the authors have any conflicts of interest associated with this study

#Corresponding author.

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