shoots with a length of (3 - 5 cm) were excised and transferred to MS medium supplemented with different concentrations of auxins such as IBA, IAA and NAA (0.1 - 2.0 mg/l) alone and in combination with KIN. In all the concentrations tried, highest number of roots was observed on MS medium with IBA 1 mg/L + 0.2 mg/L KN. Highest rooting frequency (85%), with a maximum number of roots (12.2 ± 0.53) was observed in this combination, results are tabulated in Table 3.

4. Conclusion

From the results it is noticeable that fungicide bavistin, ethylene inhibitor silver thiosulphate does not shows any harmful effect on shoot regeneration of Asclepias curassavica. Thus these promissory compounds may be useful as a media supplement to develop efficient protocols for in vitro propagation as it favors the shoot formation. Casein hydrolysate can be used as an important growth factor that promotes shoot regeneration frequency of Asclepias curassavica in the present study. Activated charcoal

Table 3. Effect of different growth adjuvants on multiple shoot regeneration from nodal explants of A. curassavica in MS media supplemented with different growth regulators. Observations: After 4 weeks (The results are mean (SE±) of 20 independent determinations).

removed the phenolic exudates completely and prevented the browning of media and thus enhanced the frequency of regeneration.

5. Acknowledgements

S Hemadri Reddy would like to thank Department of Biotechnology, S.V. University, Tirupati, Andhra Pradesh for providing Doctoral fellowship to complete this work.

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NOTES

*Corresponding author.

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