World Journal of AIDS
Vol. 2  No. 2 (2012) , Article ID: 19741 , 8 pages DOI:10.4236/wja.2012.22015

Virologic and Lipoprotein Changes after Halving Ritonavir Boosting in HIV-Infected Patients Stabilized on Once-Daily Fosamprenavir plus Abacavir/Lamivudine

Dushyantha T. Jayaweera1, Gary E. Pakes2

1University of Miami Miller School of Medicine, Miami, USA; 2GlaxoSmithKline, Research Triangle Park, USA.

Email: DJayawee@med.miami.edu

Received November 3rd, 2011; revised December 15th, 2011; accepted March 14th, 2012

Keywords: fosamprenavir; HIV infection; lipoproteins; ritonavir

ABSTRACT

Background: The effect of reducing ritonavir boosting doses on the efficacy and safety of fosamprenavir-based regimens has not been well studied. Methods: In a 52-week, phase 4, open-label, single-center pilot study, 26 antiretroviral-naïve, HIV-infected patients with viral loads >1000 copies/mL received induction with fosamprenavir/ritonavir 1400 mg/200 mg plus abacavir/lamivudine 600 mg/300 mg once daily for 28 weeks. Patients achieving a viral load <50 copies/mL at week 28 were given maintenance therapy for 24 subsequent weeks with half the ritonavir dose (100 mg) plus the usual fosamprenavir, lamivudine, and abacavir doses. Results: The study population (n = 26) was diverse with respect to sex (14 females/12 males) and race (16 black, 10 white). Baseline median viral load was 4.93 log10 copies/mL and CD4+ count 110/mm3. Of 12 induction/maintenance completers, 10 (83%) achieved viral loads <50 copies/mL by maintenance-week 24. Median CD4+ count increased from 110/mm3 at baseline to 292/mm3 at induction-week 28 and to 296/mm3 at maintenance-week 24. The incidence of adverse events at maintenance-week 24 did not differ from that at induction-week 28 (P > 0.05). Median fasting total-cholesterol, LDL-cholesterol, and triglycerides remained below NCEP cut-off levels. Baseline/induction-week 28/maintenance-week 24 median total-cholesterol was 130/177/183 mg/dL, LDL-cholesterol 78/107/114 mg/dL, HDL-cholesterol 33/41/43 mg/dL, total-cholesterol: HDL-cholesterol ratio 3.9/4.3/4.3, and triglycerides 93/145/119 mg/dL. During induction, total VLDL/chylomicron, LDL, and HDL particles increased; during maintenance, VLDL/chylomicron particles decreased, but LDL and HDL particle concentrations did not notably change. Conclusions: Reducing ritonavir boosting from 200 mg to 100 mg once daily in HIV-infected patients stabilized on once-daily fosamprenavir/abacavir/lamivudine resulted in maintenance of virologic suppression, enhanced CD4+ count, and improved triglycerides.

1. Introduction

Fosamprenavir, the phosphate ester prodrug of the antiretroviral protease inhibitor amprenavir, is frequently used as a component of combination antiretroviral therapy in HIV-infected patients. Fosamprenavir-containing regimens offer the flexibility of dosing either once-daily or twice-daily without regard to food or fluid requirements [1]. Once-daily fosamprenavir regimens can be prescribed only for antiretroviral-naïve patients and must be co-administered with low-dose ritonavir. The latter serves to inhibit the CYP3A4 hepatic metabolism of amprenavir, thereby increasing amprenavir plasma concentrations/exposure, elimination half-life, and antiretroviral activity [2].

The initial once-daily ritonavir boosting dose to be approved in the United States for co-use with fosamprenavir was 200 mg. This approval was based on the findings of the SOLO study, a clinical trial that evaluated fosamprenavir/ritonavir 1400 mg/200 mg once daily plus abacavir/lamivudine 300 mg/150 mg twice daily and showed that 69% of 322 antiretroviral-naïve patients treated were able to achieve a viral load <400 copies/mL at 48 weeks [3]. As ritonavir is associated with doserelated adverse gastrointestinal events and unfavorable lipid changes [4-6], using the lowest ritonavir boosting dose to push amprenavir concentrations to well within the clinically effective range would be expected to optimize the tolerability of fosamprenavir/ritonavir regimens. In view of this, a ritonavir boosting dose lower than 200 mg once daily—100 mg once daily—was investigated in studies of patients receiving once-daily fosamprenavircontaining regimens [7-16].

COL10053 showed that once-daily ritonavir 100 mg boosting of fosamprenavir 1400 mg provided a mean plasma amprenavir trough concentration (Ct) of 0.86 µg/mL [17], which is 6-fold higher than the mean amprenavir protein binding-adjusted 50% inhibitory concentration (IC50) for wild-type virus (0.146 μg/mL) [18] and 2.5- fold above the historical Ct value observed with unboosted fosamprenavir 1400 mg twice daily (0.35 μg/mL) [19]. The results of this pharmacokinetic study led to Food and Drug Administration approval of ritonavir 100 mg once daily for boosting fosamprenavir 1400 mg oncedaily-based regimens in treatment-naïve HIV populations in the United States [20].

There are limited data available describing whether changes in efficacy or safety occur when HIV-infected patients stabilized on a fosamprenavir/ritonavir 1400 mg/ 200 mg once-daily regimen have their ritonavir boosting dose reduced to 100 mg once daily. The purpose of COL101295 was to explore the clinical sequelae of such a switch over 24 weeks when it follows 28-week treatment induction with fosamprenavir/ritonavir 1400 mg/200 mg once daily plus abacavir/lamivudine 600 mg/300 mg once daily in antiretroviral-naïve, HIV-infected patients. In addition, an analysis of the effect of treatment on lipid particles was conducted.

2. Methods

In this 52-week, phase 4, open-label, single-center pilot study, 26 antiretroviral-naïve, HIV-infected patients with viral load >1000 copies/mL and any CD4+ cell count received induction treatment with fosamprenavir/ritonavir 1400 mg/200 mg plus abacavir/lamivudine 600/300 mg once daily for 28 weeks. Patients who achieved a viral load <50 copies/mL at week 28 were given maintenance therapy for 24 subsequent weeks with half the ritonavir dose (100 mg) plus the usual fosamprenavir, lamivudine and abacavir doses. Fosamprenavir was administered as two 700-mg tablets of Lexiva® (GlaxoSmithKline, Research Triangle Park, North Carolina), abacavir 600 mg as two 300-mg tablets of Ziagen® (GlaxoSmithKline, Research Triangle Park, North Carolina), lamivudine as two 150-mg tablets of Epivir® (GlaxoSmithKline, Research Triangle Park, North Carolina), and ritonavir as one or two 100-mg soft-gel capsules of Norvir® (Abbott Laboratories, North Chicago, Illinois).

The primary study endpoints were the proportion of patients achieving a viral load <50 copies/mL at the end of the induction and maintenance periods, and change from baseline in CD4+ count and safety/fasting lipid profile at these time points. Viral load (HIV-1 RNA) and CD4+ were measured at baseline (week 0), weeks 4, 12, 16, 20, 24 and 28 (induction period), and at weeks 32, 40, 48, and 52 (maintenance period) using the Roche Amplicor MONITOR Ultrasensitive assay (version 1.5; LLOQ 50 copies/mL) (Roche Diagnostics, Branchburg, New Jersey) and HIV-1 MONITOR Version 1.0 polymerase chain reaction assay (LLOQ, 400 copies/mL) (Roche, Nutley, New Jersey). Virologic failure was said to occur, and require the patient discontinuing their study treatment regimen, if viral load remained >200 copies/ mL at induction-week 24 or if there were two consecutive viral load levels of ³1000 copies/mL observed within a 1- to 2-week period after at least two consecutive (separated by 1-2 weeks) viral load values of <200 copies/mL). CD4+ T lymphocyte cell count was assessed by flow cytometry.

Patients were monitored for adverse events, laboratory abnormalities, and any HIV-related illnesses at baseline (week 0) and weeks 4, 12, 16, 20, 24, 28, 32, 40, 48, and 52. NMR (LipoMed Inc., Cambridge, Massachusetts) was used to measure the quantity and size of LDL, HDL, IDL, and VLDL/chylomicron particles at baseline, week 28 (end of induction), and week 52 (end of maintenance). Adherence was assessed by pill count (pills counted in returned vials of study medication) by the study site personnel at each patient visit and by patient self-report. Statistics were primarily descriptive in the observed population, and included change from baseline in viral load, CD4+ count, lipids, and lipoproteins, and tabulation of adverse events and abnormal laboratory values. A Fisher exact test was performed to compare the total adverse event incidence at week 28 of the induction regimen with that at week 24 of the maintenance regimen (week 52 of study). A P value of <0.05 for this comparison was considered statistically significant.

3. Results

3.1. Patient Characteristics

The baseline characteristics of the patient population are shown in Table 1. Fifteen patients completed the 28- week induction phase, and 12 completed both the induction and maintenance study phases. Fourteen patients

Table 1. Characteristics of the study population.

prematurely discontinued due to loss to follow-up (6), protocol violation (4), moving away (2), or suspected abacavir-related hypersensitivity reaction (2).

3.2. Efficacy

In the 15 patients who completed the induction phase, 11 (73%) had a viral load <50 copies/mL and 12 (80%) a viral load of <400 copies/mL at induction-week 28. In the 12 completers of both treatment phases, 10 (83%) patients had a viral load level <50 copies/mL (Figure 1(a)) and 11 (92%) a viral load of <400 copies/mL by maintenance-week 24 (Figure 1(b)). CD4+ count increased from a baseline median of 110/mm3, to 292/mm3 at induction-week 28, and finally to 296/mm3 at maintenance-week 24 (Figure 1(c)).

(a)(b)(c)

Figure 1. Change in viral load with respect to proportion of patients achieving HIV-1 RNA <50 copies/mL (a) and <400 copies/mL (b); Change in CD4+ cell count is shown in (c).

3.3. Safety

Adverse events generally were reported during the first 4 weeks of the study, with few reports subsequently. Potentially drug-related adverse events included abdominal discomfort (1), anemia (1), depression (1), odynophagia (1), rash (3), hypercholesterolemia (1), and hypertriglyceridemia (2) during induction, and hypercholesterolemia and hypertriglyceridemia (2) during maintenance. The incidence of adverse events at maintenance-week 24 did not differ from that at induction-week 28 (P > 0.05). Lipid analysis showed that median fasting total-cholesterol, LDL-cholesterol, and triglycerides remained below National Cholesterol Education Program (NCEP) cut-off levels [21] (Figure 2). No hypolipidemic medications were prescribed during the study. The greatest increase in median total-cholesterol levels occurred between baseline and induction-week 28 (from 130 to 177 mg/dL), as was the case with LDL-cholesterol (from 78 to 107 mg/dL), HDL-cholesterol (from 33 to 41 mg/dL), triglycerides (from 93 to 145 mg/dL), and the total-cholesterol: HDL-cholesterol ratio (from 3.9 to 4.3) (Figure 3). After reducing the ritonavir boosting dose to 100 mg once daily, little or no change was seen over the ensuing 24-week maintenance period in median total-cholesterol (+6 mg/dL [to 183 mg/dL at week 24]), LDL-cholesterol (+7 mg/dL [to 114 mg/dL]), HDL-cholesterol (+2 mg/dL [to 43 mg/dL]), and total-cholesterol: HDL-cholesterol ratio (no change, remaining 4.3), although triglycerides fell notably (−26 mg/dL [to 119 mg/dL]).

3.4. Lipoprotein Particle Analysis

Table 2 compares lipid particle concentrations at baseline, end of induction, and end of maintenance. Between baseline and the end of the induction phase, total VLDL/ chylomicron particles doubled in quantity and subclasses of these particles increased by 62% to 166%. Following

Figure 2. Lipid concentrations at baseline, induction-week 28, and maintenance-week 24. Horizontal lines above the columns are the established cut-off concentrations for each type of lipid [21].

(a)(b)

Figure 3. Changes from baseline in total cholesterol (a); LDL-cholesterol (b); HDL-cholesterol (c); and triglycerides (d).

reduction in the ritonavir dose to 100 mg once daily, VLDL/chylomicrons decreased by 8% to 46% by maintenance-week 24, the range of reduction differing by particle size. Similarly, during the induction phase, total LDL particles and LDL subclasses increased by 34 to 108%, but these either decreased (as was the case with large LDL and IDL particles) or did not change (observed with total, small, medium-small, and very small LDL particles) following the decrease in ritonavir boosting dose. HDL particles and subclasses increased during induction by 27% to 113%; following ritonavir dose reduction, total and large HDL particle quantity did not change notably, but medium HDL particles decreased by 43% and small HDL particles increased by 16%. Mean VLDL, LDL, and HDL particle size did not change from baseline during either the induction or maintenance phases of the study.

4. Discussion

The results of our study show that in HIV-infected patients stabilized on fosamprenavir/ritonavir 1400 mg/200 mg plus abacavir/lamivudine 600 mg/300 mg once daily, reduction in the ritonavir boosting dose to 100 mg once daily with no other changes in the regimen maintains virologic suppression and CD4+ cell enhancement over the ensuing 24 weeks. These findings corroborate those of two other studies that investigated the clinical effect of halving ritonavir boosting to 100 mg once daily in fosamprenavir regimens [15,16]. In the larger, statistically powered study, LESS, 229 previously antiretroviral-naïve patients stabilized (viral load <400 copies/mL for at least 3 months) on fosamprenavir regimens boosted by 200 mg daily (200 mg once daily or 100 mg twice daily) were randomized 2:1 to fosamprenavir/ritonavir 1400 mg/100 mg once daily (n = 140) or to continuation of their current regimen with no change in background therapy (n = 69) [15]. At week 24 post-switch, virologic and immunologic responses were similar in the ritonavir 100 mg and 200 mg once-daily-boosted arms: viral load <50 copies/mL in 92% versus 94% (missing or discontinuation equals failure analysis); frequency of virologic failure 8% versus 6%; median CD4+ cell count 444 vs 453 cells/mm3 above baseline. Similarly, in the direct comparative clinical trial APV109141, intent-to-treat-exposed: observed analysis showed viral load <50 copies/mL achieved by virtually the same proportion of antiretroviralnaïve patients on fosamprenavir plus abacavir/lamivudine regimens boosted by ritonavir 100 mg once daily and 200 mg daily (as a 100 mg twice-daily regimen)—

Table 2. Changes in lipid particle concentrations during the study.

94% (84/89) and 93% (84/90), respectively—with a comparable median increase from baseline in CD4+ count of 149 and 140 cells/mm3, respectively [7].

In our study, we saw no differences in the frequency of adverse events between the end of the ritonavir 200 mg once-daily-boosted induction phase and the end of the 24-week ritonavir 100 mg once-daily-boosted maintenance phase (P > 0.05). A similar absence of notable differences in drug-related adverse event frequency was also observed in the LESS study over 24 weeks following the lowering of ritonavir boosting doses of fosamprenavir regimens from 200 mg once daily to 100 mg once daily (overall 7% versus 4%, diarrhea 3% versus 1%) [15]. This lack of a difference in tolerability between the study phases may have been due in part to patients having been on induction treatment for many weeks and the known reduction in adverse events, especially gastrointestinal tolerability, that takes place over time [22]. The time-bound nature of certain adverse events was observed in the 48-week SOLO clinical trial in which a substantial proportion of patients on fosamprenavir/ritonavir 1400 mg/200 mg plus abacavir/lamivudine once daily who initially experienced drug-related nausea (31%), diarrhea (29%), vomiting (15%), and headache (10%) had abatement of these symptoms after a mean of 80, 114, 19, and 92 days, respectively, usually without the need for remedial drug therapy (in 82% to 95% of cases) [3]. In contrast, in studies that have directly compared fosamprenavir/ritonavir 1400 mg/200 mg once daily and 1400 mg/100 mg once-daily regimens, the ritonavir 100 mg regimen generally has been associated with fewer gastrointestinal side effects [7,17]. Thus, in APV109141, over 48 weeks, a lower proportion of HIV-infected patients taking fosamprenavir/ritonavir 1400 mg/100 mg once daily plus abacavir/lamivudine (n = 106) than patients taking fosamprenavir/ritonavir 700 mg/100 mg twice daily plus the same nucleoside backbone (n = 106) reported Grade 2 or higher drug-related adverse events in general (26% vs 36%) and diarrhea in particular (<1% vs 12%) [7]. Furthermore, in a crossover pharmacokinetic study (COL10053) that compared 14- day regimens of fosamprenavir/ritonavir 1400 mg/100 mg once daily and fosamprenavir/ritonavir 1400 mg/200 mg once daily in 41 healthy volunteers, the ritonavir 100- mg-boosted regimen was associated with a lower frequency of gastrointestinal adverse events in general (35% vs 56%) and nausea in particular (11% vs 27%) [17].

The overall fasting lipid profile observed during both phases of the study showed favorable HDL-cholesterol increases balanced by unfavorable increases in totaland LDL-cholesterol, with the latter two lipids nevertheless remaining below the NCEP cutoff concentrations. Reduction in ritonavir boosting of the fosamprenavir 1400 mg once-daily regimen from 200 mg to 100 mg once daily led to a modest reduction in triglycerides, but little change in total-, LDL-, and HDL-cholesterol. This finding is consistent with the results of LESS at 24 weeks post-switch from ritonavir 200 mg to 100 mg once-daily boosting (median triglycerides −21 mg/dL, total cholesterol: −1 mg/dL, LDL-cholesterol: no change; HDLcholesterol: −1 mg/dL) [15], and of TELEX II at 4 weeks post-switch (median triglycerides −35 mg/dL, total-cholesterol −17 mg/dL, HDL-cholesterol +2 mg/dL) [16]. The more favorable effect on triglycerides of a oncedaily ritonavir 100 mg plus fosamprenavir 1400 mg regimen over the fosamprenavir regimen boosted by double the ritonavir dose was also noted in the direct comparative study APV109141 [7]. That study showed at 24 weeks only slightly less elevation in total-cholesterol (+46 vs +55 mg/dL median change from baseline) and LDL-cholesterol (+37.5 vs +47.2 mg/dL median change from baseline), but notably less elevation in triglycerides (+40 vs +75 mg/dL).

In the lipoprotein analysis, the reduction we observed in total VLDL/chylomicrons and all subclasses of VLDL lipoprotein after the ritonavir 200 to 100 mg once daily switch coincided with the fall in triglycerides. This was to be expected because VLDL is the primary carrier of triglycerides, and so reduction in the former could lead to reduction in the latter [23]. Overall, this, taken along with the 16% decrease in small HDL particle concentration and the 44% and 21% reductions in LDL and IDL particle concentrations, respectively, could be perceived as positive changes from a cardiovascular risk perspective [23]. However, optimism regarding these changes must be tempered by the fact that the ritonavir 200 to 100 mg switch did not alter the concentrations of small, medium-small, or very-small LDL, which would have been desirable in view of their association with atherogenicity [23].

We did not evaluate how the switch from ritonavir 200 mg once daily to 100 mg once daily could have impacted cardiovascular inflammation biomarkers. However, in a crossover study that compared 14-day regimens of ritonavir 100 mg once daily and twice that ritonavir dose (given as 100 mg twice daily) in healthy volunteers not taking other drugs, ritonavir 100 mg once daily was associated with less elevation in the vascular inflammation biomarker sCD40L in plasma (12% vs 19%) [24]. Ritonavir 100 mg twice daily, but not ritonavir 100 mg once daily, significantly increased the biomarker adipophilin. The ritonavir 100 mg once-daily and 100 mg twice-daily regimens did not differ notably with respect to magnitude of change in HDL-cholesterol (−6% vs −10%) or in CD36 (−14% vs −16%). Neither regimen had any impact on the concentrations of the biomarkers hsCRP or sICAM-1.

Our study did not include a pharmacokinetic analysis to verify the attainment of virologically suppressive amprenavir concentrations with the ritonavir 100 mg once-daily boosting regimen. However, LESS showed little difference in the amprenavir Ct achieved at week 24 in patients taking the fosamprenavir/ritonavir 1400 mg/ 100 mg once-daily, 1400 mg/ 200mg once-daily, or 700 mg/100 mg twice-daily regimens (1.84, 1.78, and 2.30 µg/mL, respectively) [15]. These Ct values are 12.6, 12.2, and 15.8-fold higher than the historical mean amprenavir protein binding (90%) adjusted IC50 for wild-type HIV (0.146 μg/mL). Therefore, plasma amprenavir concentrations produced with the fosamprenavir/ritonavir 1400 mg/100 mg once-daily regimen in our study were expected to be several fold above this IC50. To date, ritonavir boosting doses below 100 mg once daily for fosamprenavir regimens have not been investigated. However, a study by Matthias et al. [25] comparing the effect of ritonavir 50 mg and ritonavir 100 mg on hepatic CYP3A activity showed a 34% lower area under the plasma concentration-time curve of the CYP3A probe substrate drug midazolam with ritonavir 50 mg. Therefore, if one were to extrapolate these findings to amprenavir, a CYP3A substrate, it appears possible that a ritonavir 50 mg boosting dose could result in subtherapeutic amprenavir concentrations in some patients.

In this clinical trial the study medications were not supplied by the sponsor. Our study was limited by its small evaluable population due to the high dropout rate caused by erratic delivery of drugs to these indigent patients through the U.S. ADAP. The difficulty to ensure availability and consistent delivery of antiretroviral medication to impoverished HIV-infected patients through the ADAP has been documented elsewhere [26]. This underscores the challenge of enrolling patients on such programs into clinical trials because program restrictions/regulations may change over time and make initially medication-eligible patients ineligible later on. Our study was also limited by the lack of information collected regarding diet and exercise, both of which can impact lipids.

In conclusion, reducing once-daily ritonavir boosting from 200 mg to 100 mg in HIV-infected patients stabilized on once-daily fosamprenavir/abacavir/lamivudine generally maintained virologic suppression, enhanced CD4+ count, and improved triglycerides.

5. Acknowledgements and Disclosures

The authors gratefully acknowledge the study participants and the staff members at the University of Miami Miller School of Medicine who assisted the study investigator. The study was supported by a financial grant from GlaxoSmithKline. The results of this study were presented in part as Abstract/Poster 99 at the Annual Meeting of the American College of Clinical Pharmacy, Louisville, Kentucky, October 19-22, 2008.

REFERENCES

  1. H. A. Torres and R. C. Arduino, “Fosamprenavir Calcium Plus Ritonavir for HIV Infection,” Expert Review of AntiInfective Therapy, Vol. 5, No. 3, 2007, pp. 349-363. doi:10.1586/14787210.5.3.349
  2. A. Hill, J. Van Der Lugt, W. Sawyer and M. Boffito, “How Much Ritonavir Is Needed to Boost Protease Inhibitors? Systematic Review of 17 Dose-Ranging Pharmacokinetic Trials,” AIDS, Vol. 23, No. 17, 2009, pp. 2237- 2245. doi:10.1097/QAD.0b013e328332c3a5
  3. J. C. Gathe Jr., P. Ive, R. Wood, D. Schürmann, N. C. Bellos, E. DeJesus, et al., “SOLO: 48-Week Efficacy and Safety Comparison of Once-Daily Fosamprenavir/Ritonavir Versus Twice-Daily Nelfinavir in Naive HIV-1- Infected Patients,” AIDS, Vol. 18, No. 11, 2004, pp. 1529- 1537. doi:10.1097/01.aids.0000131332.30548.92
  4. S. A. Danner, A. Carr, J. M. Leonard, L. M. Lehman, F. Gudiol, J. Gonzales, et al., “A Short-Term Study of the Safety, Pharmacokinetics, and Efficacy of Ritonavir, an Inhibitor of HIV-1 Protease,” New England Journal of Medicine, Vol. 333, No. 23, 1995, pp. 1528-1533. doi:10.1056/NEJM199512073332303
  5. G. Gatti, A. Di Biagio, R. Casazza, C. De Pascalis, M. Bassetti, M. Cruciani, et al., “The Relationship between Ritonavir Plasma Levels and Side-Effects: Implications for Therapeutic Drug Monitoring,” AIDS, Vol. 13, No. 15, 1999, pp. 2083-2089. doi:10.1097/00002030-199910220-00011
  6. M. Sension and P. J. Piliero, “Ritonavir-Boosted Protease Inhibitors: Impact of Ritonavir on Toxicities in TreatmentExperienced Patients,” Journal of the Association of Nurses in AIDS Care, Vol. 18, No. 1, 2007, pp. 36-47. doi:10.1016/j.jana.2006.11.003
  7. G. Carosi, A. Lazzarin, H. Stellbrink, G. Moyle, S. Rugina, S. Staszewski, et al., “Study of Once-Daily Versus TwiceDaily Fosamprenavir Plus Ritonavir Administered with Abacavir/Lamivudine Once Daily in Antiretroviral-Naïve HIV-1-Infected Adult Subjects,” HIV Clinical Trials, Vol. 10, No. 6, 2009, pp. 356-367. doi:10.1310/hct1006-356
  8. C. B. Hicks, E. DeJesus, L. M. Sloan, M. G. Sension, D. A. Wohl, Q. Liao, et al., “Comparison of Once-Daily Fosamprenavir Boosted with Either 100 or 200 mg of Ritonavir, in Combination with Abacavir/Lamivudine: 96- week Results from COL100758,” AIDS Research and Human Retroviruses, Vol. 25, No. 4, 209, pp. 395-403.
  9. S. De Wit, B. Poll, C. Necsoi and N. Clumeck, “Fosamprenavir Boosted with a Single 100 mg Capsule of Ritonavir as Part of a Once Daily First Line Regimen in Naïve Patients,” 8th International Congress on Drug Therapy in HIV Infection, Glasgow, 12-16 November 2006, p. 17.
  10. P. Muret, D. Montange, D. Bettinger, J. Faller, B. Martha and G. Beck-Wirth, “Assessment of Amprenavir Plasma Cmin Levels in Patients Receiving Once-Daily Fos-Amprenavir in Combination with Either 100 or 200 mg Ritonavir,” 8th International Workshop on Clinical Pharmacology of HIV Therapy, Budapest, 16-18 April 2007, p. 26.
  11. K. Y. Smith, W. G. Weinberg, E. De Jesus, M. A. Fischl, Q. Liao, L. L. Ross, et al., “Fosamprenavir or Atazanavir Once Daily Boosted with Ritonavir 100 mg, Plus Tenofovir/Emtricitabine, for the Initial Treatment of HIV Infection: 48-Week Results of ALERT,” AIDS Research and Therapy, Vol. 5, No. 5, 2008, pp. 1-10.
  12. G. Blick, P. Greiger-Zanlungo, V. Plasencia, S. Gretz, J. Han, D. DuPree, et al., “Long-Term Efficacy and Safety of Fosamprenavir (FPV) 1400 mg Once Daily (QD) Boosted by Ritonavir (r) 100 mg QD in AntiretroviralNaïve HIV+ Patients and in Antiretroviral-Experienced Patients Switched to FPV/r Due to Intolerance to Prior Regimens: BOLD100 (COL109766),” Annual Meeting of the American College of Clinical Pharmacy, Denver, 14-17 October 2007, p. 110E.
  13. R. Hsu, K. Walker-Reed and E. Acosta, “Fosamprenavir (FPV) with Low-Dose Ritonavir (RTV) Once Daily (QD) in HIV-Infected Subjects,” 7th International Workshop on Clinical Pharmacology of HIV Therapy, Lisbon, 20-22 April 2006, p. 71.
  14. E. Latuada, M. Lanzafame, C. Grosso, F. Soldani, F. Corsini, S. Storato, et al., “Optimal Fosamprenavir Regimen to Prevent Lipid Abnormalities,” Acta Bio-Medica, Vol. 80, No. 3, 2009, pp. 200-202.
  15. C. Cohen, E. DeJesus, A. La Marca, B. Young, L. Yau, L. Patel, et al., “Similar Virologic and Immunologic Efficacy with Fosamprenavir Boosted with 100 mg or 200 mg of Ritonavir in HIV-Infected Patients: Results of the LESS Trial,” HIV Clinical Trials, Vol. 11, No. 5, 2010, pp. 239-247. doi:10.1310/hct1105-239
  16. D. A. Parks, H. C. Jennings, C. Taylor, G. E. Pakes and E. P. Acosta, “Steady-State Amprenavir, Tenofovir, Emtricitabine, and Ritonavir Pharmacokinetics before and after Reducing Ritonavir Boosting of a Fosamprenavir/Tenofovir/Emtricitabine Regimen from 200 mg to 100 mg Once Daily (TELEX II),” HIV Clinical Trials, Vol. 10, No. 3, 2009, pp. 160-167. doi:10.1310/hct1003-160
  17. P. J. Ruane, A. D. Luber, M. B. Wire, Y. Lou, M. J. Shelton, C. T. Lancaster, et al., “Plasma Amprenavir Pharmacokinetics and Tolerability Following Administration of 1400 Milligrams of Fosamprenavir Once Daily in Combination with Either 100 or 200 Milligrams of Ritonavir in Healthy Volunteers,” Antimicrobial Agents and Chemotherapy, Vol. 51, No. 2, 2007, pp. 560-565. doi:10.1128/AAC.00560-06
  18. M. Sale, B. M. Sadler and D. S. Stein, “Pharmacokinetic Modeling and Simulations of Interaction of Amprenavir and Ritonavir,” Antimicrobial Agents and Chemotherapy, Vol. 46, No. 3, 2002, pp. 746-754. doi:10.1128/AAC.46.3.746-754.2002
  19. R. Wood, K. Arasteh, H-J. Stellbrink, E. Teofilo, F. Raffi, R. B. Pollard, et al., “Six-Week Randomized Controlled Trial to Compare the Tolerabilities, Pharmacokinetics, and Antiviral Activities of GW433908 and Amprenavir in Human Immunodeficiency Virus Type 1-Infected Patients,” Antimicrobial Agents and Chemotherapy, Vol. 48, No. 1, 2004, pp. 116-123. doi:10.1128/AAC.48.1.116-123.2004
  20. Food and Drug Administration, “FDA Approves Administration of LEXIVA with Lower Dose of ‘Boosting’ Medication Ritonavir,” GlaxoSmithKline, Inc., Mississauga, 2007. http://us.gsk.com/ControllerServlet?appId=4&pageId=402&newsid=1158
  21. National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III), “Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III): Final Report,” Circulation, Vol. 106, No. 25, 2002, pp. 3143-3421.
  22. T. Hawkins, “Understanding and Managing the Adverse Effects of Antiretroviral Therapy,” Antiviral Research, Vol. 85, No. 1, 2010, pp. 201-209. doi:10.1016/j.antiviral.2009.10.016
  23. J. D. Brunzell, M. Davidson, C. D. Furberg, R. B. Goldberg, B. V. Howard, J. H. Stein, et al., “Lipoprotein Management in Patients with Cardiometabolic Risk: Consensus Conference Report from the American Diabetes Association and the American College of Cardiology Foundation,” Journal of the American College of Cardiology, Vol. 51, No. 15, 2008, pp. 1512-1524. doi:10.1016/j.jacc.2008.02.034
  24. S. Collot-Teixetra, F. De Lorenzo, L. Waters, C. Fletcher, D. Back, S. Mandalia, et al., “Impact of Different LowDose Ritonavir Regimens on Lipids, CD36, and Adipophilin Expression,” Clinical Pharmacology and Therapeutics, Vol. 85, No. 4, 2009, pp. 375-378. doi:10.1038/clpt.2008.243
  25. A. Mathias, S. West, J. Hui and B. P. Kearney, “DoseResponse of Ritonavir on Hepatic CYP3A Activity and Elvitegravir Oral Exposure,” Clinical Pharmacology and Therapeutics, Vol. 85, No. 1, 2009, pp. 64-70. doi:10.1038/clpt.2008.168
  26. I. V. Bassett, C. Farel, E. D. Szmuilowicz and R. P. Walensky, “AIDS Drug Assistance Programs in the Era of Routine HIV Testing,” Clinical Infectious Diseases, Vol. 47, No. 5, 2008, pp. 695-701. doi:10.1086/590936

Appendix

ADAP: AIDS drug assistance program CD36: Scavenger receptor HDL: High-density lipoprotein hsCRP: Highly specific C-reactive protein IC50: 50% Inhibitory concentration IDL: Intermediate-density lipoprotein LDL: Low-density lipoprotein LLOQ: Lower limit of quantitation NCEP: National cholesterol education program NMR: Nuclear magnetic resonance sCD40L: Soluble CD40 ligand sICAM-1: Soluble intercellular adhesion molecule 1 VLDL: Very low-density lipoprotein