Journal of Biomaterials and Nanobiotechnology
Vol.4 No.1(2013), Article ID:27009,10 pages DOI:10.4236/jbnb.2013.41010

Surface Modification of Titanium Plate with Anodic Oxidation and Its Application in Bone Growth*

Sahar A. Fadl-allah1,2, Mohsen Quahtany1, Nahla S. El-Shenawy3,4#

1Materials and Corrosion Lab (MCL), Faculty of Science, Taif University, Taif, KSA; 2Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt; 3Zoology Department, Faculty of Science, Taif University, Taif, KSA; 4Zoology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.

Email: #elshenawy_nahla@hotmail.com

Received October 9th, 2012; revised November 15th, 2012; accepted December 16th, 2012

Keywords: Anodic Oxidation Treatment; Electron Diffraction X-Ray; Titanium; Titanium Oxide; Histology; Radiograph

ABSTRACT

Using implants for dental applications are well-accepted procedures as one of the solutions for periodontal defect repair. Suitable design and materials, their reaction with the surrounding hard tissues and interfacial biomechanical properties are still considered to be the primary criteria which need to be addressed. The purpose of present study was to evaluate the bone repair around pure titanium implants and porous surface using anodic oxidation technique, after their insertion in tibiae of rats (n = 15). Five animals received pure titanium-surface implants in tibia, 5 rough-surface implants (TiO2/Ti) in tibia and last five acted as control group. The interfacial integrity and compositional variation along the interface were studied using scanning electron microscope (SEM) with energy dispersive analysis of X-ray (EDX) and histopathology after 2 months. The rats were sacrificed 8 weeks after surgery and fragments of the tibiae containing the implants were submitted to histological analyses to evaluate new bone formation at the implant-bone interface as well as the tibiae were radio graphed. The SEM-EDX results confirmed the initial stability for the Ti implant, but the regeneration of new bone formation was faster in the case of TiO2/Ti implant, and hence could be used for faster healing. The results of the histological analysis showed that osseointegration occurred for both types of implants with similar quality of bone tissue. In conclusion, the porous-surface implants contributed to the osseointegration because they provide a larger contact area with surface roughness at implant-bone interface can help into the formation of physico-chemical bondage with the surrounding hard tissues.

1. Introduction

Pure titanium and titanium alloys are the most used biomaterials for fabrication of surgical implants due to their excellent mechanical properties, biocompatibility [1] and resistance to corrosion [2]. They are considered ideal materials because they have shown better acceptability by human tissues than other metals under diverse circumstances [1]. The discovery of relatively inert metals and alloys has led to the increasingly widespread use of metal implants in orthopedics and dentistry. Pure titanium and titanium alloys are the most frequently used materials for osseointegrated dental and orthopedic implants because of their biocompatibility [3-5]. Biocompatibility is attributed to the formation of a protective layer of titanium dioxide (TiO2) that avoids direct contact between the implant and its milieu [1]. This protective layer reduces the reactivity of the metal. Ti without any surface treatment is bioinert, to further improve the bioactivity and biocompatibility of titanium; various types of surface modification method have been explored [6]. So that several efforts were directed to the modification of metal surfaces which are often employed as a mean of controlling tissue-titanium interactions and shortening the time of bone fixation [6,7].

The high biocompatibility of titanium derives partially from the stable and protective oxide layer, which apparently aids in connecting extracellular matrix to the implant surface [1]. The knowledge of the biomaterial-bone tissue interface is extremely important to define which material would promote a better tissue response and which kind of surface would be more adequate for the proliferation of bone cells [8]. After placement of an implant in the surgical cavity, several cellular events take place. Ideally, these events should lead to wound healing by intimate apposition of the bone to the biomaterial, i.e., osseointegration [9].

Regardless of their external shape of the implants, microscopically they can present smooth, porous or textured surfaces [10-15]. Several studies have shown that the success or failure of surgical implants can be related to chemical [13,16] and biological properties [16] of their surfaces as well as to their micromorphology [17] The differences in the microstructure of implant surfaces seem to influence stress distribution, bone retention, cellular response on its surface and consequently the osseointegration [16,18-20].

Porous implants have been developed to be stabilized by bone ingrowth into the pore [13,15]. Oliveira et al. [19] determined that, although the fabrication process parameters have been optimized, the ideal porous requirements for surgical implants have not yet been reached. These authors reported that some changes are necessary in order to increase porosity and advocated that an analysis of pore size distribution along the sample has been performed to indicate more efficiently which porous fraction would better meet implant requirements [21]. Togni et al. [22] studied the histomorphometric analysis of bone tissue repair in rabbits after insertion of titanium screws under different torque. We report a successful creation of TiO2 nano-porous surfaces that mimic biomineralized matrices [23]. Therefore, the purpose of present study was to analyze, by histological methods, the bone repair over pure titanium implants with nano-porous TiO2 surface, after their insertion in tibiae of rats.

2. Materials and Methods

2.1. Coating Preparation of Titanium Plate (Anodic Oxidation Treatment)

Titanium foil (Sigma-Aldrich Chemie GmbH, Riedstr. 2D-89555 Steinheim 497329 970) with 0.25 mm thick, 99.7% metals basis was used as base material in this study. The exposed metal surface (area: 1 cm2) of each specimen was ground with silicon carbide paper to 2000 grit, washed in distilled water and then rinsed with alcohol before implantation [23]. As a result of this mechanical polishing process, Ti plate samples were prepared to be implanted in rats. Anodic oxidation treatment was used to prepare TiO2 plate sample. The electrolyte used in this work contained 1 M H2SO4 + 0.5 wt% NaF. Nano oxide coat from TiO2 was obtained using a twoelectrode system. Rectangular samples of titanium area 1 cm2 were used as the anode and a platinum plate of area 3 × 3 cm2 was used as the cathode. The operations involved were in the following sequence: a) mechanical polishing; b) the metallic sheet was then cleaned ultrasonically in acetone; and c) anodizing at 20 V in the electrolytic bath for 20 min as a result of this process, TiO2 samples were prepared which appear as nano porous layer [23] and were used for implantation.

2.2. Morphology Characterization and Chemical Composition of Examined Samples

The surface morphology and chemical composition of the untreated and treated titanium samples, Ti, TiO2/Ti-plate, before and after implantation process were studied by scanning electron microscopy (SEM) with electron diffraction X-ray (EDX) system by JEOL-840 Electron prop micro analyzer.

2.3. Animals

Fifteen male, adult Sprague-Dawley rats were used in this study and were purchased from King Fahed Medical Research Centre in Jeddah (Kingdom of Saudi Arabia). The average weight of the animals at surgery was 224 g; after 8 wk of osseointegration the average weight was 375 g. This weight gain is normal in healthy male rats. As described below, titanium implants were implanted in tibia of each animal. The un-operated tibia was used as histological control. For the entire experimental period two or three animals were kept in each cage with an unlimited supply of fresh water and rodent pellets. The European Community Directive (86/609/EEC) and National rules on animal care have been followed.

2.4. Implants

Experimental implants were manufactured from pure titanium. The implants had an overall length of 3 mm. A 1.0 mm thickness; smooth middle section had a diameter of 1.0 mm. The implants were cleaned using oscillating ultrasound equipment after placing them in n-butanol within a glass container. They were processed two times for 10 min each time, with a change of liquid. The objects were then rinsed three times and processed another 10 min in 70 percent ethanol. From this stage, in order not to contaminate the titanium surface, the implants were kept in a dry glass container. Finally, the implants, together with all necessary instrumentation, were moist-sterilized at 134˚C for 40 min.

2.5. Anesthesia and Surgery Technique

The animals were anesthetized intraperitoneally with a solution of 8 mg ketamine chlorlhydrate and 1.28 mg xylazine per 100 g body weight. The skin of right tibiae was shaved before a 1.5 cm incision was made along the tibial crest. The region of surgery surface was cleaned with antiseptic. The subcutaneous tissue, muscles and ligaments were dissected to expose the lateral external surface of the diaphyseal bone. An end-cutting bur was used to drill make a crack 1.5 mm in diameter with manual rotating movements to avoid overheating and necrosis of the bone tissue [24]. Plates of uncoated and coated Ti of 3.0 ×1.0 × 1.0 mm were implanted in tibia of rats.

2.6. Tissue Collection and Histological Examination

In vivo biocompatibility of TiO2/Ti coating was determined by implanting in the plates in rat tibia. The animals were anesthetized with the same procedure used for implant surgery. The rats were sacrificed after eight weeks of healing and the bone specimens with uncoated Ti and coated Ti implants were retrieved. The Tibiae were removed and all specimens were X-rayed using dental equipment. A hydrated aluminum chloride solution (7 percent, w/v) with formic acid (5 percent, v/v), HCl (8.5 percent, v/v), and distilled water was used to decalcify the bone specimens. The bone became sufficiently soft after 2 - 3 d in this solution at 4˚C. Phosphate buffer rinse stopped the decalcification process. The specimens were fixed in 10% phosphate-buffered formalin (pH 7.25) for 10 days and dehydrated in graduated ethanol (70% for 30 min, 95% for 30 min, and 100% for 2 × 1 h) series. After embedding samples in Spurr’s resin, each undecalcified implant block was sectioned perpendicular to the implant surface using a low speed diamond saw [25]. After polishing, the sections were stained by heamatosaline and eosin stain and observed under light microscope (Olympus BH-2, Olympus America Inc., NY, USA) with a digital camera (DS-55M-L1; Nikon) [26].

3. Results

3.1. Surface Analysis of Ti Samples before and after Anodization Process

The results of SEM micrographs, Figures 1(a) and (b), of Ti samples before and after anodization, respectively show that: a) the surface appearance of the mechanically polished pure Ti sample (uncoated) represented the typical morphology of native oxide film, with thin and nonporous structure; b) the anodic oxide film, TiO2, showed that the surface of Ti specimen (coated) appear as the network forms with nano porous slots. The EDX spectrum of untreated and treated titanium spacemen’s were presented in Figures 2(a) and (b), which indicated that the chemical composition of both samples oxide layer is Ti in addition to oxygen and small percentage from fluoride for only anodized Ti sample. The surface analysis results of untreated and anodized Ti samples confirmed that the major element present on the surface is Ti.

3.2. Photograph and X-Rays Finding

All implants had characteristic signs of bone ingrowth in various regions along their length. There were apparent differences in the amount or distribution of bone in

Figure 1. Scanning electron microscope (SEM) micrographs of the Ti specimens before and after electrochemical anodization treatment: (a) uncoated Ti specimen; (b) anodized coated Ti specimen.

Figure 2. Energy dispersive X-ray analysis (EDX) micrographs of the Ti specimens before and after electrochemical anodization treatment: (a) uncoated Ti specimen; (b) anodized coated Ti specimen.

growth between the uncoated and coated Ti evaluations (Figures 3(a) and (b)). Densification of bone immediately adjacent to the porous fiber metal, suggestive of bone ingrowth and load transfer, was observed locally at coated TiO2/Ti (Figure 3(b)) while, the Ti plate was not cover completely after two months of implantation (Figure 3(a)). No visible cracking can be seen in the tibia bone after 8 wk of TiO2/Ti-plate implantation. X-rays of tibia specimens taken at 8 wk (Figures 4(a)-(c)) showed that all implants were within the modularly cavity and all were intact as compared to control.

3.3. Microstructure of Ti Samples after Implantation Process

The results of SEM micrographs of different examined samples after implantation process (Figure 5) show that new bone formation was not similar in both groups, a good bone healing is appear on TiO2/Ti sample (coated implant). So there was new bone formation around the anodized coated implant leading to osseointegration (Figure 5(b)). In the case of Ti-implantation the new bone formation was noticed on the sides of plate only (Figure 5(a)). The results of EDX micrographs (Figure 6) of examined implanted samples show that: a) the presence of Ca, P on Ti implant sample; b) The presence of Ca, P,  

Figure 3. The photograph of the uncoated (a) and coated TiO2/Ti plates (b) after 8 wk of implantation. Red arrow points to the place of implantation.

Figure 4. Radiograph for the bone: (a) control; (b) after implantation of TiO2/Ti and (c) after implantation of Ti. The light line in the bone indicates to the plate of implantation.

Figure 5. Scanning electron microscope (SEM) micrographs of the examined specimens after implantation process: (a) uncoated Ti specimen; (b) anodized coated Ti specimen.

and O, the essential ingredients of bone cells on anodized titanium implant sample, TiO2/Ti. It is clear that an array of TiO2 nano-porous structure well adherent on Ti implant surface can be useful for accelerated bone growth in orthopedic/dental applications. We noticed that the Ca peaks are detected in two implantation process this pointed to the bone healing occur either with Ti or TiO2 /Ti but with a good proliferation with TiO2/Ti as the arrows indicate in Figure 6(b).

3.4. Animals and Implants

All animals presented satisfactory postoperative results, without any evidence of inflammation or infection of the

Figure 6. Energy dispersive X-ray analysis (EDX) micrographs of the examined specimens after implantation process: (a) uncoated Ti specimen; (b) anodized coated Ti specimen.

surgical site. No adverse reaction was observed during the procedure. After implant insertion, slight initial limping was noticed in some animals, but no pronounced motion disorders were seen; neither were there signs of infection, failure to thrive, or other complications. The structure of bone surrounding the titanium implants appeared normal after 8 wk of intramedullary osseointegration. There were obvious signs of bone remodeling adjacent to the proximal implant plate including changes in the size and shape of the bone and osteoclast activity resulting in new bone lamellae.

3.5. Histological Findings

Histological analysis of the cross sections from all tibiae confirmed the presence of bone ingrowth after 4 and 8 weeks of implantation of Ti-plate (Figure 7). The most proximal section, where there was incomplete filling of the tibia or where there had been inaccurate sizing during operative preparation of the canal, often had regions of fibrous-tissue encapsulation. Superficially, it was possible to observe a thin fibrous capsule covering the bone graft. Adjacent to the fibrous capsule it was possible to notice the bone graft with its osteocyte lacunae containing basophilic nucleus. Several bone lacunae were found into the grafts containing multinucleate cells. Deeply and adjacent to the bone surface of the recipient site, there were several resorption areas with a large number of osteoclastic-type multinucleate cells (Figures 7(b) and (c)). It was still possible to observe the absence of a bone union between the bone graft and the recipient site Figures 8(a) and (b) show the cortical osteotomy site of bone after two months implantation of TiO2/Ti-plate. Few fibroblasts could be identified, with a predominance of resorption areas concerning new bone formation areas. Discrete areas of new bone formation could be seen in the recipient site. Figure 9 shows the evascularization at

Figure 7. Photomicrographs of H&E stained histological sections from bone after implantation of Ti-plate; (a) and (b) after 0 and 4 wk of implantation, respectively, where the peripheral region of graft; (c) after 8 wk of implantation bone (white arrow); bone-implant interface where new bone formation with primary bone tissue (asterisks) and original magnification (×20).

Figure 8. Photomicrographs of H&E stained histological sections from bone after implantation of TiO2/Ti plate, (a) after 4 wk and (b) after 8 wk (×20).

the osteotomy site of bone two months after implantation of TiO2/Ti-plate. Enlarging the haversian canals (HC), but several canals show no evidence of repair. The vast majority of the chondrocytes appear viable, with only small patches of a cellular matrix. There is no evidence of an inflammatory response. Figure 10 shows the reactive cartilage that is undergoing endochondral ossification; cartilage is growing (expanding) toward the left and cartilage with hypertrophying chondrocytes (Cc) and the condroblast (Cb).

4. Discussion

4.1. New Bone Healing on TiO2/Ti/Bone Interface

Osseointegration is fundamental process in orthopedic. Several literatures explained about the integration of the implant with adjacent bone and tissue [27-29]. Osseointegration defined as the process of formation of new bone and bone healing. The incapability of an implant surface was improved to join with the adjacent bone and other tissues through the formation of a fibrous tissue around the implant and promote loosening of the prostheses. Thus, materials with a proper surface are extremely essential for the implant to integrate well with the sur-

Figure 9. Revascularization at the osteotomy site. A photomicrograph showing the cortical osteotomy site of bone two months after implantation of TiO2/Ti-plate (hematoxylin and eosin, ×10). Enlarging the haversian canals (HC), but several canals show no evidence of repair. The vast majority of the chondrocytes appear viable, with only small patches of a cellular matrix. There is no evidence of an inflammatory response.

Figure 10. Photomicrographs shows reactive cartilage that is undergoing endochondral ossification after implantation of TiO2/Ti plate for 2 months; cartilage is growing (expanding) toward the left and cartilage with hypertrophying chondrocytes (Cc) (at right) however, the condroblast (Cb) at left (A, ×10 and B, ×40).

rounding bone. Surface chemistry, roughness and topography are all parameters that influence both the osseointegration and biocompatibility [30]. So, good biocompatibility and rapid osseointegration are essential factors of prolonged stability of the implant material.

Since Ti and its alloys has won as a good metallic biomaterial, researchers were keen to further improve the osseointegration of Ti by applied different surface modification method by altering the nature of the surface [30- 33]. Recently, TiO2 has been suggested as a bioactive surface to improve the osseointegration process. The advantage of using TiO2 is that it can be grown directly on the Ti surface, by cost-effective techniques such as anodic oxidation [34-36]. Also, it is well known that one problem with bone healing is poor adhesion strength at the Ti/bone interface [37-40]. By using anodic oxidation, TiO2 is formed with a chemical bond between the oxide and Ti substrate that likely results in enhanced adhesion strength of the bone.

Porous TiO2 films with controlled nanostructures were prepared reproducibly and conveniently by potentiostatic anodic oxidation in different electrolyte [41-43]. Our previous research has shown that it is possible to increase the range of titanium in medical application by depositing a porous layer of TiO2 on the metal surface [44-46]. The objectives of the present work are to assess the effect of TiO2 coat prepared 1 M H2SO4 + 0.5 wt% NaF to obtain a new anodized titania to evaluate histological effect of Ti/bone and TiO2/Ti/bone interfaces and to contribute clinically relevant data on the permanence of titanium metal structures used in osteosynthesis in the body.

The oxide film formed in 1 M H2SO4 and 0.5 wt% NaF has higher nano porous structure compared with our previous work that formed in 0.5 M H2SO4, and that formed in 1.4 M H3PO4 [44], Figure 1(b) predicated that a good healing are occurring on nano-TiO2/Ti/bone interface surface.

By comparing the tibiae of implanted uncoated Ti with that of coated anodized Ti, note that there is a different in the size of two implanted tibiae. The coated implanted tibiae are larger than the uncoated implanted tibiae (Figures 3(a) and (b)). This result is considered as a good observation and may be attributed to the excellent bone healing process on TiO2/Ti/bone interface and perhaps draws the authors to further future study. The detection of both implanted tibiae by X-ray investigation indicated that the high bone regeneration on coated implanted tibiae need more time to get a normal bone shape without any apparent defect (Figure 4(c)).

Electrochemical formation and characterization of porous titanium (TiO2) films [46], which eventually causes the adhesion of bone cells, albeit at a much slower quantity than the TiO2 nano porous surface investigated in this work (Figure 5). The bone cells show a spreading morphology and network formation on the coated anodized Ti surface. This result confirmed that the nano-scaled bioactive TiO2 nanostructures incorporated in this investigation form strongly bonded and stable nano-porous layer, which can increase the bone cells on its surface and reduce the interfacial fracture [47]. A close look at the areas surrounding the cells confirms that the nano pores are being filled in with bone matrix. These results are fully compatible with the findings of researchers [48]. The surface properties of biomaterials play a critical role in the establishment of cell-biomaterial interfaces [49].

4.2. Histological Evaluation of Ti Implant Samples

Microscopic observation of the implant/bone interface at this time-point indicated successful osseointegration with normal remodeled bone adjacent to the fixture [20]. The presence of corrosion products has been found in blood analysis [50]. In some cases the products of corrosion were found around the blood vessels, in keeping with the findings of Meachim and Williams [51] and Torgersen et al. [52], in a histological study of soft tissue adjacent to titanium implants. The observation of metal particles located intracellular or in association with vessels may represent a biological response aimed at eliminating the foreign material [52,53]. The properties and quality of the implant material, the shape of the implant and the handling and surgical procedure are of crucial importance for an optimal biological performance of any implant device [1]. Titanium dioxide is generally considered to be of low toxicity [1,50].

Brånemark has studied the processes of osseointegration for endosseous titanium implants in long bones under various conditions [53]. The present results indicated that histological analysis of all fixtures showed a direct bone contact with the titanium surface at the resolution level of the light microscope as described before [53]. In our study, plate implants with coated porous surface (TiO2/Ti) were compared to plate implants with uncoated surface regarding the new bone formation on the implant-bone interface after implantation in rat tibiae. The results showed no difference of new bone quality between both types of implants. However, when the quantity of bone new formation at implant-bone interface was evaluated, a larger formation of bone tissue was observed for the TiO2/Ti-surface implants. The most important factors to implant osseointegration are related to the characteristics of its surfaces, which include topography and chemical and electric properties of the material [54], since boneimplant interaction is mainly related to the most external layers of the implants [55]. Important factors to a more successful osseointegration are: implant material, implant shape, surgical technique [15], quantity of bone tissue [15], load [56] and implant resistance [4]. However, some other factors such as surface energy, sterilization techniques and chemical and topographic properties of the implant surface are extremely important for the final outcome of osseointegration [15,50,57].

Bone growth is also dependent on factors such as percentage of surface porosity and the presence of gaps between the implant and the bone at the time of placement [15]. Therefore, in order to obtain osseointegration, the surgical cavity must be prepared with the least injury possible [15,50,58]. In order to cause minimal damage to the surrounding bone tissues, in the present study, bone perforation was performed using burs of increasingly larger diameters, and under constant saline irrigation. After that, the implants were gently pressed into the surgical cavity, which diminished the gap between the implant and the bone and promoted efficient stability.

Some previous studies used a 4-week healing period to evaluate the biocompatibility of metal materials [15,59]. Healing periods were longer than 4 weeks added no benefits to increase the quantity of bone tissue ingrowth into porous-surface implants, and observed that only bone tissue maturation took place after this period [15]. Thus, in the present study, an 8-week period was used to evaluate the biocompatibility of TiO2/Ti-surface grade as compared to commercially pure Ti-implants fabricated by means of anodic oxidation technique. The purpose of studying and developing TiO2/Ti-surface implants is to promote a more stable and biocompatible fixation of titanium implants. The creation of a TiO2 surface aims not only at increasing contact area but also at allowing bone ingrowth into the pores, including those located more centrally. Such ingrowth is due to pore intercommunication, which produces a three-dimensional net and allows a mechanical entanglement [15,60]. More bone formation was significantly observed in the TiO2-surface implants. The results of this study showed that because of the larger contact surface promoted by the presence of pores, there was more bone ingrowth on the implant-bone interface. Such results are consistent with those of Deporter et al. [60], Karabuda et al. [58] and Zinger et al. [61] who also observed more effectiveness of the porous-surface implants compared to other types of implants.

Our results show improvement in cell attachment and spreading on TiO2/Ti coated as compared to uncoated Ti, which is in line with previous studies [6,62]. During the initial period of bone healing, the mesenchymal cells move into the inflamated site and differentiate into osteoblasts, which allow the osteoid formation. The presence of mesenchymal cells in abundance at the healing zone between the TiO2-coated implant and the mature bone indicates the commencement of bone regeneration [6]. In our study, formation of osteoid on the TiO2/Ti coated implant surface confirms the cellular activity required for the new bone formation. These results are in accordance with earlier studies which show faster new bone formation on hydroxy apatite coated implants [6,63]. The skeleton is a metabolically active organ that undergoes continuous remodeling throughout life. Bone remodeling involves the removal of mineralized bone by osteoclasts followed by the formation of bone matrix through the osteoblasts that subsequently become mineralized [64]. The remodeling cycle consists of three consecutive phases: resorption, during which osteoclasts digest old bone; reversal, when mononuclear cells appear on the bone surface; and formation, when osteoblasts lay down new bone until the resorbed bone is completely replaced. Bone remodeling serves to adjust bone architecture to meet changing mechanical needs and it helps to repair microdamages in bone matrix preventing the accumulation of old bone. It also plays an important role in maintaining plasma calcium homeostasis [65].

5. Conclusion

According to the methodology employed in this study, it was possible to conclude that, the anodic oxidation technique improved the surface of titanium by forming a film of nano-porous oxide layer. This porous-surface improved the osseointegration process because it encourages the bone healing at TiO2/Ti/bone interface. Therefore, the results showed that the roughness TiO2/Ti implant surface is better than the smooth Ti surface and well tolerated when placed in rat tibiae, thus corroborating the findings of previous studies that indicated modified titanium plate as the best biomaterial for bone surgical implants.

6. Acknowledgements

This research was supported by funding source from Center of Research Excellence in Corrosion, King Fahd University of Petroleum and Minerals, Al-Read, KSA. This study was parts of the Grant No. CR-12-2010.

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NOTES

*The author declares that there are no conflicts of interest.

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