ABSTRACT

The semen quality of 479 men in infertile marriages, who attended the infertility clinic of OOUTH, Sagamu between May 2004 and April 2009 were analyzed. The semen samples were physically examined, microscopically analyzed and aerobically cultured. The result showed that out of 51.5% oligospermic cases recorded, 39.8% could be classified severe. Cryptozoospermia were diagnosed in 72 (15%) cases. Bacteriospermia and Necrozoospermia were found in 149 and 6 cases respectively. The predominant organism isolated was Staphylococcus aureus (50.32%) while Neisseria gonorrhoea was isolated in only 2 cases. Highest infection of 62.4% was noticed among the group with 1% - 19% motility. From the studied population, 8.8% were azoospermic. The commonest morphological defects were pin and ragged heads (48%). It is therefore obvious that the assessment of semen quality remains one of the important steps in infertility interventions.

1. Introduction

The coming together of a man and a woman in marriage in the Nigerian socio-cultural context is primarily for procreation. Anxiety sets in when this is not achieved within a stipulated period of time. It is often followed by emotional stress and domestic strife. It also creates social strains with friends, family members and colleagues [1, 2].

Research has provided much insight into the feelings of individual and the social consequences of infertility [3].

Infertility in the general context is described as a situation where a couple does not succeed in achieving pregnancy in spite of unprotected sexual intercourse over a period of 12 months [2,4]. In male, infertility is defined as the inability of the male reproductive cells to produce mature, actively motile and functional spermatozoa in sufficient amount that will ensure fertilization of a released ovum in the fallopian tubes [5]. Usually, the woman bears the blame of infertile marriages, especially when the man is able to achieve normal erection and ejaculation. Virility is thus thought to be synonymous with fertility [6,7]. Since the quality of seminal fluid cannot be physically assessed, intervention of the medical laboratory is often sought.

Infertility is a global problem affecting approximately 10% - 15% of married couples. Global variation has been reported in the incidence of infertility from as low as 1% to 1.5% in Korea and Thailand to as high as 13% - 23% in urban areas of Columbia and rural areas of New Guinea. The prevalence of infertility is particularly high in sub-Saharan African, varying from 20% - 40% in some parts of West Africa. The World Health Organization reveals that male reproductive capacity is deficient in not less than 50% of infertile couples in several countries of the world. In Africa, up to 65% of gynaecological consultations are for infertility [2,8,9].

There are many factors that are associated with infertility in man. These include; the presence of varicocele, sexual dysfunction, genitourinary infections, urospermia, age and nutrition. Other factors include stress and emotions, endocrine and chromosomal abnormalities, excessive alcohol consumption, environmental factors and non-diagnosable causes. Labour migration also separates husband and wife, hence reducing exposure of the wife to sexual intercourse [10-13].

In diagnosing infertility in male, Computer-Assisted Sperm Analysis (CASA), which allows an objective assessment of different cell characteristics, is in use in some parts of the world [14].

However, in many parts of Nigeria, semen analysis is routinely done through the conventional microscopy method. Culture, hormonal evaluation and special sperm function tests (sperm-cervical mucus interaction, sperm penetration assay etc.) are beneficial. For a clinician, evaluation of seminal quality is linked with a desire to predict potential fertility, identify causes of infertility or detect changes in potential fertility [2,14].

Onemu and Ibeh found that the presence of bacteria in semen (bacteriospermia) might be an early warning signal (EWS) to taking important steps in the prevention and management of male infertility [15].

This study was designed to assess the quality of seminal fluid of men in infertile marriages who attended the gynaecology clinic of Olabisi Onabanjo University Teaching Hospital, Sagamu-Nigeria between May 2004 and April 2009, with a view to identify the possible contribution of the male factor to overall infertility problems in this environment.

Olabisi Onabanjo University Teaching Hospital (OO UTH) is strategically located at Sagamu, where it serves patients from all parts of the State and neighbouring states of Lagos, Ondo and Oyo. Infertility cases constitute 14.8% of all gynaecological consultations in this hospital [16].

2. Materials and Methods

The results of seminal fluid analysis of men being investigated in infertile marriages at Olabisi Onabanjo University Teaching Hospital, Sagamu, Ogun State, SouthWest of Nigeria were collated. The study involved all males who attended the infertility clinic of the hospital with their mates between May 2004 and April 2009, a period of five years. A total of 479 men were, investigated.

Pre-production counseling was given to all the participants. These include abstinence from sexual intercourse or masturbation for a period of 3 - 5 days and avoiding the use of antibiotics prior to collection especially in cases where culture is required. Agreement was equally reached with each participant on the mode of production and time lapse between production and analysis of specimen [14].

About 85% of the samples were collected through masturbation, while others by coitus interruptus. The samples were asceptically collected into sterile universal bottles provided by the hospital. The semen samples were then examined for physical parameters e.g. colour, volume, viscosity, coagulation and liquefaction. The percentage motility and sperm concentration were calculated according to WHO approved standard [14]. All samples were examined within an hour after production. The samples were stained for morphological assessment using Giemsa stain.

The samples were further examined bacteriologically. They were cultured on freshly prepared heated blood agar (in duplicate) and MacConkey agar plates. A set of the inoculated heated blood agar plates was incubated in an increased carbon dioxide (10%) environment. Others were incubated aerobically at 37˚C for between 24 to 48 hours.

The bacteria growths were identified using standard methods [17]. The antibiogram of the isolates were determined by agar diffusion method. 

3. Results

A total of 479 men were examined for the quality of their seminal fluid. The mean of the first two analysis carried out within 4 - 6 weeks were used for the purpose of this study. A total of 94 (19.6%) and 385 (80.4%) cases presented with primary and secondary infertility respectively. The analysis of sperm concentration is shown in Table 1 where 39.7% of the total attendee had sperm count of 20 million cells per milliliter of seminal fluid and above. Forty-two men were azoospermic. Assessment result of the percent motility, an important factor in assessing seminal fluid quality is shown in Table 2. From this table, it is noticed that six men were necrozoospermic. However 181 (41.42%) had motility of 50% and above. The morphological defects vary between 65% - 75% in 392 of sample examined. The most common defects were ragged heads and broken tails. Ninety-three out of the 149 bacteriospermic cases recorded in this study falls within 1% - 19% motility range as shown in Table 3. It is equally important to note that 4 (66.7%) of azoospermic cases were equally infected. From Table 4, Staphylococcus aureus was isolated in 78 (50.32%) semen samples, Candida albicans in 8 and Neisseria gonorrhoea in 2.

4. Discussion

This study showed that 8.8% of the study populations were azoospermic. This is the only group one can categorically say may be fully responsible for the couple's infertility, since they are sterile.

About 51.5% whose sperm concentrations were below 20 × 10⁶ cells/ml could equally be a source or contributory to infertility. This might as well confirm the statement that about half of infertility cases have their origin in the male counterpart [2,8].

Table 1. The sperm concentration of attendees in millions/ml.

Table 2. The motility assessment of semen of attendees expressed in %.

Table 3. Seminal fluid infection in relation to motility.

Table 4. Microorganism isolated from the attendees’ semen samples.

Olatunji and Sule-Odu recorded 48.2% from an earlier study in the hospital [16]. The slight increase noticed here might have to do with more people resulting to solving their infertility problems through orthodox means. In a similar study carried out at the Eastern part of Nigeria, 60% of the male were Oligospermic, 13% was recorded in Tunisia while 45.3% was recorded in lIorin, North-Central part of Nigeria [6,12,18-20].

The variation noted above may be a matter of concern. Although geographical differences had been reported to have no major effect on semen quality [21], other factors may need to be considered, such as the pattern of normal sperm count in the areas of study.

Semen analysis though routinely used to evaluate the male partner in an infertile marriage, sperm measurements that discriminate between fertile and infertile men are not well defined [22].

Infection has remained one of the important factors in infertility especially among the secondary infertility group. Pathogenic micro-organisms were isolated in 47.1% of semen samples of men in infertile marriages in Benincity, 7% in Ilorin and 16% in Enugu [6,12,15,18].

In this study, 32.4% of semen samples examined were infected. The isolation of Staphylococcus aureus in 50.3% of the infected cases might be associated with body hygiene of the couples involved. However, crossinfection cannot be ruled out as few couples confessed cases of “trying outside marriage”. This finding agrees with other published works [15,23].

Relationship between low sperm motility and infection becomes obvious in this study as 88.6% asthenozoospermic cases were infected. This group may benefit from simple therapeutic procedures. This often becomes very difficult, due to economic status of many in this group. The cost of drugs, duration of medication, follow up investigations and cultural belief are few of the obstacles that might work against achieving fertility even in these diagnosed cases. The 2.7% azoospermic cases with infection in this study will benefit from adequate treatment at least to have good health and reduce cross-infection.

Greater percentage of cases in this study (80.4%) presented with 2˚ infertility, this agrees with the work of Nwabuisi and Onile. The higher cases of 2˚ infertility might be due to post infective causes which are common in Africa [2,18].

Most causes of infertility due to male factor are reversible if clinician’s prescriptions are religiously followed and patients are well informed. This education should include information on how STI’s cause infertility in women and men at anytime in their reproductive years.

5. Limitation

The supply of antibiotic sensitivity discs were inconsistent (variations in product types), hence the antibiotic sensitivity pattern of the isolates could not be collated.

6. Acknowledgements

The authors are grateful to all the members of staff of the Department of Medical Microbiology and Parasitology of OOUTH for maintaining consistent analytical method and proper record keeping. Your support is appreciated.

REFERENCES

NOTES

^*Corresponding author.