Background: Hepatitis B is an infectious disease, which is a main way of vertical transmission of infectious HBV between mother and infant. Hepatitis B virus infection is always a hot topic of social concern, especially in China. The paper studies hepatitis B virus in maternal blood, breast milk, saliva of hepatitis B virus infection model (HBV-M) in Hefei city, Anhui province, PRC. HBV-DNA load and related data in Hefei city are used for risk assessment of the transmission of hepatitis B virus to provide evidence for evidence-based medicine and scientific guidance of infant feeding patterns. Methods: On the principle of informed consent, inpatient hepatitis B maternal blood 695, breast milk, saliva 614,169 copies were used as the object of analysis, using the ELISA method for the detection of HBV-M, using real-time fluorescence quantitative PCR detection of HBV-DNA load. We analy ze HBsAg in saliva, milk, the positive rate of HBV-DNA and HBV-M in serum, saliva, milk, and explore the positive rate of HBV-DNA and serum HBV-DNA load correlation. Results: At the age of 18 - 44 years old perinatal women, HBV-DNA positive rates of maternal serum, breast milk, saliva were 157 cases in A group HBsAg, HBeAg positive: 99.36%, 88.06%, 96.77%; in 312 cases in group B, HBeAb HBsAg, HBcAb positive: 17.63%, 2.93%, 54.67%; 69 cases in C group HBsAg, HBcAb positive: 63.77%, 27.27%, 28.57%; D group of 71 patients with simple HBcAb positive: 12.68%, 3.13%, 0%; E group and 86 cases in control group HBVM: 1.16%, 0%, 0%. A ccording to the serum and milk testing of Group A and Group B, HBV-DNA chi-square is χ2 = 237.45, P < 0.01 ; there is a significant difference in serum and saliva; HBV-DNA chi-square χ2 = 289.49, P < 0.01, the difference has statistical significance. Conclusion: 1) HBV-DNA load high maternal blood, breast milk, saliva are potentially persistent hepatitis B virus infection risk, especially infectious blood . 2) Of maternal milk, saliva and blood HBV-DNA HBV-DNA load were positively correlated (r = 0.96; P < 0.01); with the serum HBV-DNA load increas ing , breast milk and saliva HBV-DNA positive rate s w e re increased and infectivity enhanced. 3) Maternal blood, breast milk, saliva specimens for any HBV-DNA ≥ 1000 copies/ml are not breastfeeding . 4) T he mother who carr ies the hepatitis B virus cannot do maternal infant feeding, and deep kiss intimate contact, in order to prevent blood, saliva and other ways of infection of hepatitis B virus . 5) Saliva testing is instead of milk inspection, because saliva is easier ;
Hepatitis B is a widespread public health infectious disease, by blood transfusion, sex, maternal and insect bites and other means of communication. HBV, neonatal infection of hepatitis B virus after 90% or more will turn for the hepatitis B virus carriers, who in adult lives can progress to cirrhosis and hepatocellular carcinoma [
Based on the principle of informed consent, from August 2008 to December 2011, 695 patients, aged at 18 to 44 years old, average age 29.6, in Hefei carrying hepatitis B virus in maternal blood, breast milk, and saliva are as research objects of the study, whom are chosen by Hefei Maternal and Child Health Hospital. Research methods in informed consent principle hospitalized hepatitis B maternal blood 695, breast milk 614, saliva 169 copies as the analysis object, using the ELISA method for the detection of HBV-M, using real-time fluorescence quantitative PCR detection of HBV-DNA load. According to maternal HBVM were divided into five groups: A group, HBsAg, HBeAg, HBcAb was positive in 157 cases; B group HBsAg, HBeAb, HBcAb was positive in 312 cases; C group HBsAg, HBcAb was positive in 69 cases; in group D (HbsAg) HBcAb was positive in 71 cases; E group: HBV-M negative in 86 cases.
Milk collection base postnatal colostrum juice 5 ml by ward nurses collected, please be willing to participate in the study of 614 cases of maternal milk nipple with sterile saline after disinfection, using sterile tubes for milk, colostrum collected 5 ml, collected in a test tube, all tubes, centrifuge tube, suction head and a storage vessel have been high pressure sterilization, specimens were collected in 4000 r/min centrifugal 10 min, abandon the fat milk, whey and middle hepatitis B virus markers and quantitative detection of HBV-DNA.
Maternal saliva collected from 2 to 4 days postpartum, early in the morning, please be willing to participate in the study of hepatitis B maternal mouth with water after Su, rest 10 minutes, abandoned to the Su mouth after the first mouth saliva, saliva secretion collection continued to 5 ml to clean the inside the tube, 4000 r/min centrifugal after 10 minutes, take saliva supernatant. All tubes, centrifuge tube, suction head and a storage container prior to use by autoclaving, all operations are strictly in accordance with the principles of asepsis of.
HBVM positive in accordance with the Beijing cosmos pharmaceutical company limited by enzyme-linked immunosorbent assay (ELISA) kit for hepatitis B specification standard microplate judgment, at the same time with the ABI series instrument real time fluorescent quantitative PCR method for detecting maternal serum, breast milk, saliva HBV-DNA load; specimens studied are all fresh specimens, HBV-DNA produced by ABI company the PE-5700 fluorescence quantitative PCR instrument, HBV-DNA PCR fluorescence reagent kit for the Xiamen Anpuli biological engineering company limited to provide, in strict accordance with the manual operation, positive judgement standard: HBV-DNA ≥1000 copies/ml as HBV-DNA positive, direct report quantitative results; the measured value HBV-DNA < 1000 copies/ml, according to the reagent specification judgment is negative, report HBV-DNA < 1000 copies/ml.
Statistical method and its application to specimens of the experimental operating system data collection, using SPSS 17 software package for data processing, a variety of specimens in HBVM, the positive rate of HBV-DNA and study data comparison, by χ2 test13.
695 Cases of maternal serum, breast milk, saliva samples were collected, a comprehensive set of relatively small, so we are faithfully reported cases of actual research, sample testing data, statistical results as shown in
From
From
ELISA HBV markers | maternal serum | breast milk | maternal saliva | ||||||
---|---|---|---|---|---|---|---|---|---|
N | sAg | HBVDNA | N | sAg | HBVDNA | N | sAg | HBVDNA | |
A HBsAg HBeAg | 157 | 157 | 156 | 134 | 115 | 118 | 62 | 57 | 60 |
B HBsAg HBeAb HBcAb | 312 | 312 | 55 | 307 | 6 | 9 | 75 | 42 | 41 |
C HBsAg HBcAb | 69 | 69 | 44 | 55 | 13 | 15 | 21 | 5 | 6 |
D HBcAb | 71 | 2 | 9 | 32 | 0 | 1 | 5 | 0 | 0 |
E Control Group | 86 | 0 | 1 | 86 | 0 | 0 | 6 | 0 | 0 |
Combined Meter | 695 | 540 | 265 | 614 | 134 | 143 | 169 | 104 | 107 |
Group | maternal serum | breast milk | saliva (copies/ml) | |||
---|---|---|---|---|---|---|
Cases | maximum | minimum | maximum | minimum | maximum | minimum |
A 157 | 7.12 × 109 | <1.00 × 103 | 4.26 × 106 | <1.00 × 103 | 2.97 × 107 | <1.00 × 103 |
B 312 | 2.41 × 107 | <1.00 × 103 | 3.65 × 104 | <1.00 × 103 | 8.72 × 105 | <1.00 × 103 |
C 69 | 1.09 × 107 | <1.00 × 103 | 2.89 × 104 | <1.00 × 103 | 6.34 × 104 | <1.00 × 103 |
D 71 | 3.18 × 105 | <1.00 × 103 | 2.17 × 103 | <1.00 × 103 | 3.56 × 103 | <1.00 × 103 |
E 86 | 1.25 × 103 | <1.00 × 103 | <1.00 × 103 | <1.00 × 103 | <1.00 × 103 | <1.00 × 103 |
Maternal serum group | Serum HBV-DNA | milk HBV-DNA | saliva HBV-DNA | ||||||
---|---|---|---|---|---|---|---|---|---|
Load (copies/ml) | N1 | + | Rate % | N2 | + | Rate (%) | N3 | + | Rate (%) |
<1.00 × 103 | 430 | 0 | 0.00 (0/430) | 380 | 0 | 0.00 (0/380) | 20 | 0 | 0.00 (0/20) |
(1.00 - 9.99) × 103 | 59 | 59 | 100 (59/59) | 49 | 1 | 2.04 (1/49) | 4 | 0 | 0.00 (0/4) |
(1.00 - 9.99) × 104 | 22 | 22 | 100 (22/22) | 19 | 5 | 26.32 (5/19) | 21 | 6 | 28.57 (6/21) |
(1.00 - 9.99) × 105 | 25 | 25 | 100 (25/25) | 20 | 11 | 55.00 (11/20) | 25 | 14 | 56.00 (14/25) |
(1.00 - 9.99) × 106 | 27 | 27 | 100 (27/27) | 23 | 16 | 69.57 (16/23) | 27 | 20 | 74.07 (20/27) |
(1.00 - 9.99) × 107 | 83 | 83 | 100 (83/83) | 77 | 65 | 84.42 (65/77) | 49 | 44 | 89.79 (44/45) |
(1 - 9.99) × 108-9 | 49 | 49 | 100 (49/49) | 46 | 45 | 97.83 (45/46) | 23 | 23 | 100 (23/23) |
Combined Meter | 695 | 265 | 38 (265/695) | 614 | 143 | 23.29 (143/614) | 169 | 107 | 63.31 (107/169) |
From
Group E, research of hepatitis B virus five for all negative in 86 cases of maternal serum, breast milk, saliva HBV-DNA diagnostic rate was 1.16% (1/86), 0% (0/86), 0% (0/86), maternal serum, breast milk, saliva HBV-DNA load is <1000 on average, latex diagnostic positive rate is 0% the control group, but due to maternal serum diagnostic positive rate was 1.16% (1/86), by tracking with the following diagnosis for the window period, regarding this kind of crowd suggested experimental monitoring of hepatitis B virus markers after making mode of lactation and maternal contact mode of evidence-based medicine.
Implementation of breastfeeding is an important measure for preventing infant health problems; WHO breastfeeding rates are above 80%. On the carriers of the HBV breastfeeding problems, domestic and foreign scholars have different views. The research shows that, HBV replication and strong delivery women such as group A HBsAg HBeAg positive serum and milk are contagious. Studies confirmed the persistence of infection of hepatitis B virus between mother and infant, breast-feeding of infants at increased risk for HBV infection, persistent infection with hepatitis B virus between mother and infant. The implementation of breastfeeding should pay attention to compliance with evidence-based medicine science sex. Hepatitis B virus nucleic acid gene is a reflection of HBV replication, infectious index [
Jin Chunzi [
Because the hepatitis B virus is mainly spread through blood, baby milk-sucking, HBV-contained blood is also likely to be inhaled. It has been reported that HBV may come into the blood circulation through the capillaries of newborn or infant’s oral cavity, pharynx, esophagus, gastrointestinal mucosa, breakage, ulcer, and milk. In occurrence of mastitis and cracked nipples, infant gastrointestinal mucosal edema, inflammation and other pathological conditions, breastfeeding should be stopped immediately; such cases could be recovered after breastfeeding [
Instead of milk inspection, saliva is relatively easy; salivary hepatitis B infection rate is slightly stronger than that of milk. Pregnancy detection of salivary HBV DNA load could provide evidence-based medicine by providing infant feeding patterns and maternal contact mode in advance [
Mothers who carry hepatitis B virus should detect maternal saliva and milk during lactation, because the parent HBV DNA content is a dynamic process. Because the viral replication is dynamic change, negative nature cannot guarantee that the whole lactation was negative. Because lactation period is nearly a year, milk and saliva of HBV-DNA load are dynamic changes of hepatitis B virus, i.e., DNA replication, infectious nature. The baby has potential infectious risk of maternal milk, saliva; blood HBV-DNA and HBV-DNA load were positively correlated (r = 0.96; P < 0.01); with the increasing of the serum HBV-DNA load and breast milk, saliva HBV-DNA positive rate is increased, infectivity enhanced. The women of childbearing age should strengthen health knowledge of hepatitis B knowledge propaganda, education, prevention measures, so as to improve the perinatal health care system.
The authors declare no conflicts of interest regarding the publication of this paper.
Huang, C.Y. and Fang, Y.B. (2019) Maternal Blood Milk Saliva Sample Selection and the Transmission of Hepatitis B Virus Infectious Research. Open Journal of Obstetrics and Gynecology, 9, 363-370. https://doi.org/10.4236/ojog.2019.93037