Several aromatic homopropargyl alcohols, and derivatives were prepared by one-pot reaction of 1,3-dilithiopropyne (or its equivalent) with aldehydes and ketones. The antibacterial activity, of alcohols thus prepared, against Gram (+) and Gram (-) bacteria was tested. This activity varies depending upon substitution on the homopropargyl alcohol unit. Apparently, the benzene ring and the free hydroxyl group (or its alcoxide) are needed, in the homopropargyl alcohol unit, to be biologically active.
Homopropargyl alcohols are very useful synthetic intermediates in organic synthesis [
We have reported a highly regioselective method for the obtention of aromatic homopropargyl alcohols, 3, from reaction of the operational equivalent of 1,3-dilithipropyne,5 (obtained from treatment of allene, 6, with n-BuLi), with aromatic aldehydes or ketones (Scheme 2) [
Scheme 1. Formation of alcohols 3 and 4 from organometallics 1 and 2.
Scheme 2. Preparation of dianion 5.
economical procedure for the preparation of 5, from the reaction of propargyl bromide, 7, with n-BuLi in the presence of TMEDA (Scheme 2) [
We recently reported [
In spite of the fact that homopropargyl alcohols have broad application in organic synthesis, no studies have been reported about its antibacterial activity. The appearance and increase of drug resistance among pathogenic bacteria represents a threat for human health [
The aim of this work is to report herein the preparation of several homopropargyl alcohols, using procedures depicted in Scheme 2 [
Scheme 3. One-pot synthesis of 4-substituted homopropargyl alcohols 10.
General Information. All glassware and syringes were dried in an oven overnight at 140˚C and flushed with nitrogen immediately prior to use. Transfers of reagents were performed with syringes equipped with stainless-steel needles. All reactions were carried out under a positive pressure of nitrogen. Nitrogen was passed through a Drierite gas-drying unit prior to use. Diethyl ether and tetrahydrofuran were refluxed and freshly distilled from sodium and potassium/benzophenone ketyl respectively, under nitrogen atmosphere. Hexane was distilled from sodium and collected and kept over activated molecular sieves. n-Butyllithium was titrated according to the method of Watson and Eastham [
Homopropargyl alcohols 11, 12, 13 and 18 were prepared from dichloropropene, 9, according to Scheme 3 as in [
2-phenyl-4-pentyn-2-yl acetate. (15) IR (film): νmax 3292, 2119, 1754, 1495, 1447, 1375, 1369, 1228, 1126 cm−1; 1H RMN (400 MHz, CDCl3): δ 7.49 (m, 2H,), 7.36 (m, 2H), 7.30 (m, 1H), 2.78 (dd, J = 2.6, 16.6 Hz, 1H), 2.71 (d, J = 2.6, 16.6 Hz, 1H), 2.19 (s, 3H), 2.06 (t, J = 2.6 Hz, 1H) 1.65 (s, 3H); MS (EI) m/z (relative intensity) 39.1 (10), 43.1 (51), 77.1 (18), 105.1 (15), 121.1 (100), 142.1 (15), 163.1 (20), 202.2 (1).
5-bromo-2-phenyl-4-pentyn-2-ol. (16) IR (film): νmax 3411, 2119, 1495, 1446, 1375, 1101 cm−1; 1H RMN (400 MHz, CDCl3): δ 7.49 (m, 2H), 7.36 (m, 2H), 7.30 (m, 1H), 2.78 (d, J = 16.6 Hz, 1H), 2.71 (d, J = 16.6 Hz, 1H), 1.65 (s, 3H); MS (EI) m/z (relative intensity) 43.1 (83), 77.1 (20), 105.1 (13), 116 (2), 121.1 (100), 222.1 (1).
5-hydroxy-5-phenyl-2-hexynoic acid. (17) IR (film): νmax 3400 - 2600, 2239, 1692, 1495, 1446, 1377, 1070 cm−1; 1H RMN (400 MHz, CDCl3): δ 12.25 (s, 1H), 7.49 (m, 2H), 7.36 (m, 2H), 7.30 (m, 1H), 2.92 (d, J = 17.4 Hz, 1H), 2.85 (d, J = 17.4 Hz, 1H), 1.65 (s, 3H).
(Z)-7-chloro-2-phenyl-6-en-4-heptyn-2-ol (18). (0.104 g, 62%); IR (KBr): νmax 3401, 2214, 1601, 1494, 1446, 1098 cm−1; 1H NMR (400 MHz, CDCl3): δ 7.51 (m, 2H), 7.36 (m, 2H), 7.27 (m, 1H), 6.34 (d, 1H, J = 7.4 Hz), 5.83 (dt, 1H, J = 2.2, 7.4 Hz), 2.95 (dd, 1H, J = 2.2, 16.9 Hz), 2.89 (dd, 1H, J = 2.2, 16.9 Hz), 2.50 (s, 1H), 1.69 (s, 3H); 13C NMR (100 MHz, CDCl3): δ 146.5, 128.4, 128.3, 127.2, 124.9, 112.1, 94.8, 78.1, 73.6, 36.1, 29.4; MS (EI) m/z (rel. intensity) 43.1 (76), 77.1 (15), 99.0 (4), 105.1 (8), 121.1 (100), 183.2 (M+ - HCl, 10) HRMS (ESI, V+): m/z [M+ - HCl]+ calc. for C13H13O 185.0966, found 185.0968.
Microorganisms tested. Cultures of Leuconostoc mesenteroides (ATCC 8293-2), Lactobacillus plantarum (ATCC 14917), Staphylococcus aureus (ATCC 25922), Listeria monocytogenes (ATCC 19116) Escherichia coli (ATCC 25923) Pseudomonas aeruginosa (ATCC 15442) and Salmonella enteritidis (ATCC 13076) were used.
Suspension preparation. Each microorganism was inoculated into trypticase soy broth (TSB) + yeast (Oxoid®) and cultured at 37× until the desired concentration was reached. The suspension of bacteria to be cultured was equivalent to 0.5 McFarland standard, (1.5 × 108 CFU/ml).
Bactericidal assays. All tests were performed by duplicate. The microorganism to be tested was uniformly spread with sterile cotton swab over MRS agar for L. plantarum and L mesenteroides and overtrypticase soy agar plate for the other bacteria. All agar plates were prepared using 20 ml of agar and allowed to solidify uniformly. 7 mm diameter holes were cut in the agar gel, 20 mm apart from each one.100 ul of inoculum suspension was swabbed uniformly in the corresponding agar plates.
Five serial dilutions of synthetic products, at concentrations of 100, 50, 25, 12.5 and 6 mg/mL were prepared. 50 μL of each dilution were added to holes. Test systems were incubated for 24 h at 35˚C under aerobic conditions except for L. plantarum and L. mesenteroides that were cultured under capnophilic conditions. The MIC was defined as the lowest concentration of the sample that prevented visible growth.
In order to compare their biological activity, we decided to prepare a series of homopropargyl alcohols unsubstituted in the acetylenic position (11, 12, 13), some salts and acetylated derivatives (14 and 15) and some 4-substituted acetylenic alcohols (16, 17, 18).
The minimal inhibitory concentration (MIC) of the different homopropargyl alcohols prepared is shown in
Compound Minimum Inhibitory Concentration (MIC)* (mg/mL)
*MIC was defined as the lowest concentration of the sample that prevented visible growth. For MIC determination, control strains included Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 49619.
lenic position to obtain derivative 16, the biological activity was enhanced, except in the case of Pseudomonas spp. In the case of Staphylococcus aureus activity of derivative 16 was twice as active as 12. Interestingly, when the hydroxyl group of alcohol 12, was acetylated (15), the activity against all the microorganisms was lost. In chloroenyne18, activity against Gram (+) bacteria was enhanced when compared to parent compound 12.
When activity of 3-butyn-1-ol, 19, was tested, it was negative against all microorganisms, showing that the presence of the aromatic ring in the homopropargyl alcohol unit is needed in order to have some biological activity.
In summary, several aromatic homopropargyl alcohols were prepared, by one-pot reaction of dianion, 5, with aromatic aldehydes and ketones. The antibacterial activity of the compounds thus prepared, against Gram (+) and Gram (−) bacteria was tested. This antibacterial activity varies depending upon substitution on the homopropargyl alcohol unit. Apparently, the benzene ring and the free hydroxyl group (or its alcoxide) are needed in the homopropargyl alcohol unit to be biologically active, as it may be concluded by comparison of activity of compounds 11, 12, 13, 14, 15 and 19. It is not clear what induces the difference in antibacterial activity between compounds 11, 12, 13 because there is no clear trend in activity between them, neither for Gram (+) nor for Gram (−) microorganisms.
The development of novel, non-toxic-food compatible chemical products could help to reduce the presence of pathogens and spoilage microorganisms from food industry environments, including surfaces, equipment and even worker hands. Further research has to be done to test toxicity of compounds in
We thank Sistema de Estudios de Posgrado (SEP-UCR) for a stipend to C.U., CIPRONA-UCR for high resolution mass spectra determination, the Bacteriology Laboratory of Microbiology Faculty, University of Costa Rica for kindly supplying the bacteria and Vicerrectoría de Investigación (UCR) for financial support.
Umaña, C.A., Arias, M.L. and Cabezas, J.A. (2017) Synthesis and Antibacterial Activity of Aromatic Homopropargyl Alcohols. International Journal of Organic Chemistry, 7, 295-300. https://doi.org/10.4236/ijoc.2017.73023