This paper reports on the assessment of the quality and quantity of castor oil from castor seeds collected from different regions in Tanzania. The castor seeds from Arusha, Dar es Salaam, Dodoma, Iringa, Kagera and Morogoro regions in Tanzania were extracted by Soxhlet method using n-hexane and the oil yields were determined. The yields were 52.78%, 49.95%, 47.89%, 44.23%, 43.71% and 43.69% for Dodoma, Arusha, Iringa, Morogoro, Kagera and Dar es Salaam respectively. The physico-chemical parameters that were determined were refractive index (1.468 - 1.473), pH 5.7- 6.3, viscosity (0.943 - 0.954), specific gravity (165.50 - 187.46 mg KOH/g Oil), saponification value (76.68 - 80 mg KOH/g Oil), iodine value (76.68 - 80.12 g I 2/100 g Oil), acid value (0.44 - 1.97 mg NaOH/g Oil), free fatty acid (0.22 - 0.99) and peroxide value (10.79 - 13.73). Fatty acid profile of castor oil was analysed using Shimadzu GCMS and ricinoleic acid ranged from 83.5% to 92.3% of the total fatty acids in the castor oils.
Castor plant is cultivated and grows in the wild throughout the tropical, warm temperate and subtropical regions between latitudes 40˚ South and 52˚ North. In Tanzania, castor plant, Ricinus communis grows as a wild plant almost all over the country [
Castor oil is a pale yellow viscous liquid with mild odour and boils at 313˚C (595˚F). It has a density and molecular weight of 961 kg/m3 (0.961 g/cm3) and 933 g/mol respectively and is normally obtained by solvents extraction or mechanical pressing of dried castor seeds. The high content of ricinoleic acid with its three functional groups of hydroxyl, carboxylate and carbon-carbon double bond (alkene) is the main reason why castor oil has versatile application possibilities in the chemical industry [
Relative to other vegetable oils, castor oil has different physical and chemical properties which vary with the method of extraction (solvent or mechanical pressing) of the oil. The castor oil that is obtained from the cold pressing method has low acid value, low iodine value, lighter color and slightly higher saponification value compared to the solvent-extracted oil [
Castor oil is used to make soap, sulfated castor oil or Turkey Red Oil which is used to manufacture detergent, and other forms of the oil are important for the treatment of leather, industrial lubricants, and other industrial uses [
The castor seeds were collected from the outskirt of Arusha, Kagera, Iringa, Dar es Salaam, Dodoma and Morogoro regions. These regions have vast geographical diversity. While the temperature in Arusha typically ranges between 5˚C and 30˚C and receives an average precipitation of about 87.7 mm per annum, the average temperature in Dar es salaam is 30˚C and an average precipitation of 95.4 mm. Morogoro has an average temperature is 30.1˚C and an average precipitation of 77.9 mm, Iringa average temperature is 24.8˚C and an average precipitation of 55.1 mm, Dodoma an average temperature is 29˚C and an average precipitation of 39.1 mm, Kagera average temperature is 26.3˚C and an average precipitation of 175.1 mm [
About 20 g of a clean sample was weighed and then dried in the oven at 80˚C for 7 hrs and the weight was recorded after every 2 hrs. The same procedure was repeated until a constant weight was obtained. After 2 hours, the sample was removed from the oven and placed in the dessicator for 30 minutes to cool then removed and re-weighed. The percentage moisture content in the seeds was calculated from the expression:
where W1 = original weight of the sample before drying; W2 = weight of the sample after drying.
In a typical extraction experiment, about 200 mL n-hexane was measured and then poured into a round bottom flask. A sample weighing 10 g was placed in the thimble and then inserted in the centre of the extractor. The solvent was heated to 70˚C and when the solvent boiled, the vapour rose through the vertical tube into the condenser at the top. The liquid condensate dripped into the filter paper thimble in the centre which contained the solid sample to be extracted. The extract seeped through the pores of the thimble and filled the siphon tube, where it flowed back down into the round bottom flask. This was allowed to continue for 3 h so as to maximize the oil yield. The experiment was repeated by placing 10 g of the sample into the thimble again [
The sample to be extracted, 10 g was placed in the thimble and 200 mL of n-hexane poured into the empty round bottom flask. The solvent was heated to 70˚C and allowed to heat for 3 h continuously while extracting the oil by using Soxhlet apparatus. Different weight of the sample, 10 g, 15 g and 20 g were added in the apparatus at 3 h intervals, solvent was evaporated using rotary evaporator and the percentage of oil determined using known procedures [
The percentage composition of fatty acids in the castor oil was determined by the Shimadzu Gas Chromatograph-Mass Spectrometer (GCMS-QP 2010 Plus) at the chemistry department using standard Fatty Acid Methyl Esters (FAMEs) [
Fatty acid methyl esters (FAME’s) for GC-MS analysis were prepared and the fatty acid composition of castor oil was determined by GCMS-QP 2010 plus, Shimadzu Japan equipped with HP capillary column and helium carrier gas. The column temperature was programmed at 120˚C with an increment of 3˚C per minute for 57 minutes whereas the injector and detector temperatures were set at 260˚C and 280˚C, respectively as reported elsewhere [
The physical properties of the liquids depend on their chemical composition, pressure and temperature. An unknown oil physical parameter can be measured and compared with the literature and standard values, the oil can then be identified.
Density bottle was used in determining the specific gravity of the oil. A clean and dry stoppered bottle of 25 mL capacity was weighed (W0) and then filled with the oil stoppered and reweighed to give (W1). The oil was substituted with distilled water after washing and drying the bottle and weighed to give (W2). The expression for specific gravity (Sp.gr) is:
where W0 = weight of dry empty density bottle; W1 = weight of density bottle + oil; W2 = weight of density bottle + distilled water [
A clean, dried Ostwald viscometer with a flow time above 200 seconds for the fluid to be tested was elected. The sample was filtered through a sintered glass (fine mesh screen) to eliminate dust and other solid materials in the liquid sample. The viscosity meter was charged with the sample by inverting the tube’s thinner arm into the liquid sample and suction force drawn up to the upper timing mark of the viscometer, after which the instrument was turned to its normal vertical position. The viscometer was placed into a holder and inserted to a constant temperature bath set at 29˚C. The oil was kept for approximately 10 minutes for it to come to the bath temperature of 29˚C. The suction force was then applied to the thinner arm to draw the sample slightly above the upper timing mark. The afflux time was recorded by timing the flow of the sample as it flowed freely from the upper timing mark to the lower timing mark [
Abbe’s refractometer was used in the determination of refractive index. This instrument measures the index of refraction by measuring the critical angle of total reflection. In this case, a few drops of the sample were transferred into the glass slide of the refractometer. Water at 30˚C was circulated round the glass slide to keep its temperature uniform. Through the eyepiece of the refractometer, the dark portion viewed was adjusted to be in line with the intersection of the cross. At no parallax error, the pointer on the scale pointed to the refractive index. The refractometer was calibrated using distilled water where the refractive index of water at that temperature was obtained [
In a dry clean 25 mL beaker, 2 g of the sample was placed followed by 13 mL of hot distilled water and the mixture was stirred slowly. The mixture was then cooled in a cold-water bath to 25˚C. The pH electrode was standardized with buffer solutions (pH 4 and 7) and the electrode immersed into the sample where an average pH of three recordings per sample were recorded [
Into a dried 250 mL conical flask was placed 5.0 g of the oil sample followed by 25 mL of absolute ethanol and 3 drops of phenolphthalein indicator. The mixture was heated in a shaking water bath for 5 minutes. While hot, it was titrated against 0.1 N KOH until pink color appeared. Vigorous shaking was done when approaching the end point to ensure thorough mixing. The volume of 0.1 N KOH consumed by an acid was recorded. The acid value was calculated as reported in the literature [
where V = volume of KOH used; N = normality of KOH and m = mass of sample.
To 0.25 g of the oil sample in a 250 mL conical flask was added 10 mL of chloroform followed by 30 mL of Hanus iodine solution. The flask was securely closed and the solution was left shaking for 30 minutes in the dark. This was followed by adding 10 mL of 15% potassium iodide solution and then shaken, after which 100 mL of distilled water was added. The mixture was then titrated with the iodine solution against 0.1 N Sodium thiosulfate solution till a yellow color formed. This was followed by addition of 2 - 3 drops of starch solution after which a blue solution formed. The titration continued until the blue color disappeared while the volume of Na2S2O3 at end point was recorded. The Iodine value (I.V) was calculated as reported by other workers [
where c = Concentration of Na2S2O3 used; V1 = vol of Na2S2O3 used for the blank; V2 = vol of Na2S2O3 used for sample; m = mass of the sample.
A one gram (1 g) sample of the oil was weighed into a 250 mL glass conical flask, and then 10 mL of ethanol- ether mixture (2:1) was added to the same flask followed by 25 mL of 0.5 N ethanolic potassium hydroxide. The flask was then fitted to a reflux condenser and refluxed using a boiling water bath for 30 min with occasional shaking. To the warm solution were added 3 - 4 drops of phenolphthalein indicator and the warm solution was titrated against 0.5 M HCl to the end point. The same procedure was used for other samples and blank. The expression for saponification value (S.V) is given by equation [
where B = the volume of the solution used for blank test; S = the volume of the solution used for determination; N = Actual normality of the HCl used; m = Mass of the sample.
Into a 250 mL Erlenmeyer flask, 1 g of the oil sample, 1 g of potassium iodide and 20 mL of solvent mixture (glacial acetic acid/chloroform, 3/2 by volume) were added and the mixture was boiled for one minute. The hot solution was poured into a flask containing 20 mL of 5% potassium iodide. Thereafter, 3 drops of starch solution were added to the mixture and the latter was titrated with 0.025 N standardized sodium thiosulphate and the peroxide value was determined following the method reported by Kyari [
where, S = mL of Na2S2O3; N = normality of Na2S2O3; W = weight of oil sample (g).
The percentage of moisture content of the castor seeds were summarized in
These values (
Fatty Acid | Tanzania | |||||
---|---|---|---|---|---|---|
DAR | ARS | IRINGA | DOM | MOR | KGR | |
Palmitic | 8.8 | 2.3 | 3.1 | 2.4 | 3.1 | 4.2 |
Stearic | 2.9 | 4.2 | 1.1 | 1.4 | 1.1 | 1.8 |
Oleic | 1.8 | 5.1 | 1.4 | 4.1 | 2.2 | 2.8 |
Linoleic | 2.9 | 4.8 | 1.9 | 4.3 | 3.3 | 4.4 |
Linolenic | T.A | T.A | T.A | T.A | T.A | T.A |
Ricinoleic | 83.5 | 83.6 | 92.3 | 87.8 | 90.6 | 86.7 |
SFA | 11.7 | 6.5 | 4.3 | 3.8 | 4.2 | 6.0 |
UFA | 88.3 | 93.5 | 95.7 | 96.2 | 95.8 | 93.9 |
CO from heavy motor vehicle trafficking in the city which subsequently results in acid rain. This acidity does not favour the uptake of nutrients which are required by the plants for the biosynthesis of various metabolites which include fatty acids and glycerols. Dar es Salaam has a very high humidity which also does not favour the biosynthesis of various fatty acids in the castor oil.
The GC-MS results shown in
Except for Kagera region, the acid values obtained from different regions compares well with literature [
Region | A.V (mg/g) | % FFA | P.V (meq/g) | S.V (mg KOH/g Oil) | I.V (g/100 g Oil) |
---|---|---|---|---|---|
Dar | 0.56 | 0.28 | 12.50 | 178.12 | 78.15 |
Arusha | 0.51 | 0.26 | 13.73 | 171.54 | 80.20 |
Iringa | 0.44 | 0.22 | 10.87 | 173.22 | 81.09 |
Moro | 0.79 | 0.40 | 11.09 | 187.46 | 81.21 |
Dodoma | 0.56 | 0.28 | 10.98 | 168.02 | 83.42 |
Kagera | 1.97 | 0.99 | 10.79 | 165.50 | 80.61 |
fatty acid and hence elevating the acid value. Iringa had the lowest acid value (0.44 mg/g oil), which is attributed to the low temperature in Iringa and probably due to the presence of strong phenolic anti-oxidants such as Butylated Hydroxyanisole (BHA) and Butylated Hydroxytoluene (BHT) [
The low levels of peroxide value obtained in this work, suggest either the presence of strong antioxidant that prevent the activity of oxygen that would otherwise spoil the oils. From
High saponification value means low molecular weight of the triglyceride and the lower the SV, the higher the molecular weight. From
The result obtained for the Iodine value for different oils are shown in
In
Region | S.G | R.I | Viscosity (dPaS) | pH |
---|---|---|---|---|
Arusha | 0.954 | 1.471 | 9.0 | 6.3 |
Dodoma | 0.945 | 1.469 | 9.2 | 6.1 |
Dar | 0.948 | 1.468 | 9.2 | 6.0 |
Morogoro | 0.945 | 1.469 | 9.2 | 6.0 |
Iringa | 0.943 | 1.473 | 9.3 | 5.8 |
Kagera | 0.949 | 1.470 | 9.1 | 5.7 |
Property | Ranges |
---|---|
Specific gravity 20/25˚C | 0.957 - 0.968 |
Refractive Index, nD | 1.476 - 1.479 |
Viscosity at 25˚C (St/dPaS) | 9.3 - 10 |
Iodine value | 82 - 88 |
Saponification value | 175 - 187 |
Free fatty acid | 0.3 - 0.7 |
Acid value | 0.4 - 4.0 |
below the ASTM standards given in
The viscosity values obtained in this study (
Due to presence of free fatty acids in the castor oil, it was necessary to determine the pH of the oil since it tells the state of oil as to whether the dissociation has taken place or not. The pH of castor oils is summarized in
This research has established that the amount of oil extracted from castor seeds collected from six regions in
Tanzania varies. For solvent extraction (Soxhlet using n-hexane), we establish that the yield of castor oil ranges from 43% to 53% depending on the origin and variety of castor seeds. The physico-chemical parameters of the castor oil are determined using standard methods and most of them are within the ASTM values and those reported elsewhere. The fatty acid profile of the castor oils reveals some disparities in the amount of fatty acids suggesting that there can be climatic and soil composition influences on the fatty acid profiles. The results show that seeds from Dodoma have the highest oil possibly due to the type of soil and the climate. The region receives a minimal amount of rainfall and has a favorable ambient temperatures ranging from 28˚C to 34˚C with very low humidity.
We would like to acknowledge Southern Africa Biochemistry and informatics of Natural products (SABINA) for partial financial support to this work. The University of Dar es Salaam Chemistry department staffs are acknowledged for their skillful technical assistance.
Athumani Omari,Quintino A. Mgani,Egid B. Mubofu, (2015) Fatty Acid Profile and Physico-Chemical Parameters of Castor Oils in Tanzania. Green and Sustainable Chemistry,05,154-163. doi: 10.4236/gsc.2015.54019