Petasin is a potential antitumor against human neuroblastoma cell SK-N-SH by inhibiting the ERK1/2 phosphorylation. In view of its great activity and new antiproliferative mechanisms, a series of petasin derivatives were designed and synthesized, which showed great antiproliferative activity. Among them compounds 1h and 1f were more effective against SK-N-SH cells than petasin with the IC 50 values of 0.87 and 2.63 μM, respectively.
Neuroblastoma (neuroblastoma, NB) [
Petasin (
contraction. In our group, the great antitumor activity against Sk-N-SH was reported for the first time [
Based on the above results, a series of petasin derivatives were designed and synthesized (
Petasin derivatives were efficiently synthesized as described in Scheme 1. Compounds 1a -h and 3 were obtained from the isopetasinol by acylation reactions with carboxylic acids or maleic anhydride [
To further investigate SARs, compounds 8a-c with an amide functional group were designed and synthesized [
The IC50 values for all obtained petasin analogues against three human cancer cell lines including SK-N-SH, MGC-803 and HepG-2 were determined using MTT assay [
Compared to petasin, the antiproliferative activities of isopetasinol against the tested cancer cell lines was decreased significantly (IC50 > 100 µM), which indicated that an additional unsaturated ester group is beneficial for the activity. Therefore, substituted benzoic ester group was introduced as represented in compounds 1a -h. Interestingly, some of these compounds showed great cytotoxicity against SK-N-SH and were much more potent than petasin and cisplatin. Compounds with electron-donating groups attached to the phenyl ring were much
Scheme 1. Reagents and conditions: 1) NaOH, CH3OH, 62˚C, 4 - 6 h; 2) DMAP, DCC, CH2Cl2, 0˚C, 6 h; 3) m- ClC6H5COOOH, CH2Cl2, rt, 3 h; 4) (C2H5)3N, CH2Cl2, 0˚C, 2 h; 5) Na2CO3, DMF, 65˚C, 2 h; 6) m-CH3C6H5SO2Cl, (C2H5)3N, CH2Cl2, 0˚C, 6 h; 7) NaN3, DMF, 95˚C, 8 h; 8) Zn, NH4Cl, THF/H2O, 2 h, rt; 9) (C2H5)3N, CH2Cl2, 0˚C, 2 h.
Compd | IC50 (µmol/L) | ||
---|---|---|---|
SK-N-SH | MGC-803 | HepG-2 | |
1a | 12.01 ± 1.27 | >100 | 38.02 ± 1.87 |
1b | 5.98 ± 1.06 | 12.99 ±2.62 | 64.17 ± 2.06 |
1c | 14.91 ± 1.85 | 12.84 ± 1.87 | 34.55 ± 3.51 |
1d | >100 | 13.73 ± 2.46 | 30.30 ± 2.43 |
1e 1f 1g 1h | 31.36 ± 2.01 2.63 ± 0.76 61.25 ± 3.21 0.87 ± 0.28 | 33.84 ± 2.85 24.83 ± 1.97 40.40 ± 3.41 >100 | 33.82 ± 2.01 57.33 ± 2,53 32.02 ± 1.98 27.46 ± 1.63 |
2a | 34.78 ± 3.56 | 43.75 ± 3.78 | >100 |
2b 2c | 76.23 ± 2.18 26.87 ± 1.87 | >100 >100 | >100 49.34 ± 1.76 |
2d | 48.95 ± 2.14 | >100 | >100 |
2e | >100 | >100 | >100 |
4a | 14.54 ± 1.43 | 43.19 ± 2.86 | 58.17 ± 3.01 |
4b | 12.76 ± 1.52 | >100 | >100 |
8a | >100 | >100 | >100 |
8b | 31.80 ± 2.07 | 36.86 ± 3.41 | 27.63 ± 2.43 |
8c | 64.31 ± 2.65 | 78.37 ± 4.01 | 43.98 ± 3.01 |
Isopetasinol | >100 | >100 | >100 |
Petasin Cisplatin | 5.76 ± 0.98 12.32 ± 1.06 | 34.41 ± 2.75 10.88 ± 1.86 | 26.84 ± 1.65 8.14 ± 0.89 |
more potent than those with electron-withdrawing groups. Among them, compound 1h and 1f showed the best cytotoxicity with the IC50 values of 0.87 and 2.63 µM, respectively, which were about 14 or 15 fold more potent than cisplatin (IC50 = 12.32 µM).
Compounds 2a -e with epoxy ring showed bad antiproliferative activity against SK-N-SH with the IC50 values more than 26 µM and were less potent than cisplatin and petasin (IC50 = 5.76 µM), which indicated that exo- double bond were important for the antiproliferative activity. Besides, the antiproliferative activity of compounds 4a-b and compounds 8a-c also become to be worse than that of compounds 1a-1h and the positive control.
In conclusion, SAR of new petasin derivatives against SK-N-SH showed that an additional ester group and the exo-double bond were important for the antiproliferative activity. Furthermore the isomerization of the double bond and unsaturated ester unaffected the antiproliferative activity. But if the ester group was substituted with amide group, the antiproliferative activity against Sk-N-SH showed a certain extent effects. And compounds 1h and 1f were discovered, which showed selective and great antiproliferative activity with IC50 values of 0.87 and 2.63 µM against SK-N-SH cells. The result in this study is valuable for the design and optimization of petasin derivative and the discovery of new antiproliferative agent.
Petasin, extracted from Ligularia fischeri with traditional ethanol reflux. Reagents and solvents were purchased from commercial sources and were used without further purification. Thin-layer chromatography (TLC) was performed on silica gel GF254 plates. Silicagel GF254 and H (200 - 300 mesh) from Qingdao Haiyang Chemical Company was used for TLC, preparative TLC, and column chromatography respectively. Melting points were determined on an X-5 micromelting apparatus and are uncorrected. 1H NMR and 13C NMR spectra were recorded on a Bruker 400 MHz and 100 MHz spectrometer respectively. High resolution mass spectra (HRMS) were recorded on a Waters Micromass Q-T of Micromass spectrometer by electrospray ionization (ESI).
A mixture of isopetasinol (1 equiv) and Carboxylic acid (1.5 equiv) in CH2Cl2 in the presence of DMAP (1 equiv) and DCC (1 equiv) was stirred at 0˚C for 6 h. The disappearance of compound isopetasinol was monitored by TLC. Upon completion, the solvent was removed and water was added, the reaction mixture was extracted with CH2Cl2. The combined organic layer was washed with brine, dried over anhydrous MgSO4 and concentrated under vacuum to afford the crude product. The crude product was purified by a flash column to yield the pure product 1a-h.
1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl-4-methylbenzoate (1a). Yellow solid, yield 75%, mp: 71.2˚C - 72.1˚C, 1H NMR (400 MHz, CDCl3) δ 7.94 - 7.25 (m, 4H, ArH), 5.81 (s, 1H, HC = C), 5.14 - 5.01 (m, 1H, CH), 2.96 (d, J = 13.7 Hz, 1H, CH2), 2.52 (d, J = 13.6, 1H, CH2), 2.42 (s, 3H, CH3 ), 2.39 - 2.26 (m, 2H, CH2), 2.23 (d, J = 11.0 Hz, 1H, CH2), 2.11 (s, 3H, CH3), 1.87 (s, 3H, CH3), 1.81 (d, J = 11.0, 1H, CH2), 1.63 - 1.54 (m, 1H, CH), 1.08 (s, 3H, CH3), 1.04 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 206.94, 191.66, 166.23, 165.15, 143.73, 143.43, 129.60, 129.11, 127.59, 127.14, 126.76, 74.01, 46.34, 42.27, 41.17, 31.65, 30.91, 30.13, 22.61, 22.13, 21.65, 17.19, 10.83. HR-MS (ESI): Calcd. C23H28O3, [M+H]+ m/z: 352.2126, found: 352.2128.
1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl-4-methoxybenzoate (1b). Yellow solid, yield 70%, mp: 107.7˚C - 110.4˚C, 1H NMR (400 MHz, CDCl3) δ 8.03 - 6.95 (m, 4H, ArH), 5.83 (s, 1H, HC=C), 5.13 - 5.04 (m, 1H, CH), 3.89 (s, 3H, CH3), 2.98 (d, J = 13.7 Hz, 1H, CH2), 2.56 (d, J = 13.7, 1H, CH2), 2.46 - 2.29 (m, 2H, CH2), 2.25 (d, J = 11.0 Hz, 1H, CH2), 2.13 (s, 3H, CH3), 1.89 (s, 3H, CH3), 1.82 (d, J = 11.0, 1H, CH2), 1.63 - 1.57 (m, 1H, CH), 1.10 (s, 3H, CH3), 1.06 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 206.99, 191.71, 165.93, 165.26, 163.45, 143.46, 131.59, 127.14, 126.72, 122.71, 113.65, 73.85, 55.46, 46.38, 42.28, 41.17, 31.70, 30.91, 30.15, 22.62, 22.14, 17.19, 10.83. HR-MS (ESI): Calcd. C23H28O4, [M+H]+ m/z: 368.2047, found: 368.2049.
1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl-3-nitrobenzoate (1c). White solid, yield 74%, mp: 128.9˚C - 132.8˚C, 1H NMR (400 MHz, CDCl3) δ 7.95 - 7.64 (m, 4H, ArH), 5.82 (s, 1H HC = C), 5.12 - 5.01 (m, 1H, CH), 2.95 (d, J = 13.7 Hz, 1H, CH2), 2.56 (d, J = 13.7 Hz, 1H, CH2), 2.47 - 2.39 (m, 2H, CH2), 2.21 (d, J = 11.1 Hz, 1H, CH2), 2.12 (s, 3H, CH3), 1.88 (s, 3H, CH3), 1.78 - 1.70 (m, 1H, CH), 1.59 (d, J = 11.0 Hz, 1H, CH2), 1.09 (s, 3H, CH3), 1.06 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 207.02, 191.53, 165.14, 164.52, 147.95, 143.63, 132.98, 131.74, 129.65, 127.88, 126.99, 123.96, 76.40, 45.85, 42.28, 41.04, 30.95, 29.94, 22.63, 22.16, 17.17, 10.79. HR-MS (ESI): Calcd. C22H25NO5, [M+H]+ m/z: 383.1807, found: 383.1809.
1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl-4-nitrobenzoate (1d). White solid yield 75%, mp: 88.1˚C - 91.4˚C, 1H NMR (400 MHz, CDCl3) δ 8.36 - 8.21 (m, 4H, ArH), 5.84 (s, 1H, HC = C), 5.17 - 5.09 (m, 1H, CH), 2.99 (d, J = 13.7 Hz, 1H, CH2), 2.58 (d, J = 13.7 Hz, 1H, CH2), 2.48 - 2.31 (m, 2H), 2.26 (d, J = 11.0 Hz, 1H, CH2), 2.14 (s, 3H, CH3), 1.89 (s, 3H, CH3), 1.86 (d, J = 11.2, 1H, CH2), 1.72 - 1.64 (m, 1H, CH), 1.11 (s, 3H, CH3), 1.08 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 206.86, 191.48, 164.32, 164.29, 150.61, 143.79, 135.67, 130.71, 126.99, 126.92, 123.60, 75.66, 46.16, 42.25, 41.13, 31.52, 30.93, 29.99, 22.65, 22.17, 17.17, 10.93. HR-MS (ESI): Calcd. C22H25NO5, [M+H]+ m/z: 383.1807, found: 383.1813.
1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl-3-fluorobenzoate (1e). White solid, yield 73%, mp: 115.1˚C - 116.7˚C, 1H NMR (400 MHz, CDCl3) δ 7.85 - 7.27 (m, 4H, ArH), 5.82 (s, 1H, HC = C), 5.11 - 5.03 (m, 1H, CH), 2.98 (d, J = 13.7 Hz, 1H, CH2), 2.57 (d, J = 13.7 Hz, 1H, CH2), 2.46 - 2.29 (m, 2H, CH2), 2.26 (d, J = 11.2 Hz, 1H, CH2), 2.12 (s, 3H, CH3), 1.87 (s, 3H, CH3), 1.82 (d, J = 11.4, 1H, CH2), 1.61 - 1.53 (m, 1H, CH), 1.09 (s, 3H, CH3), 1.05 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 206.99, 191.60, 164.90, 143.60, 127.04, 126.87, 125.31, 120.23, 120.01, 116.58, 116.35, 74.83, 46.21, 42.25, 41.13, 31.55, 30.94, 30.05, 22.64, 22.15, 17.17, 10.87. HR-MS (ESI): Calcd. C22H25FO3, [M+H]+ m/z: 356.1863, found: 356.1865.
1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl-2-hydroxybenzoate (1f). White solid, yield 73%, mp: 128.8˚C - 134.6˚C, 1H NMR (400 MHz, CDCl3) δ 10.81 (s, 1H, OH), 7.84 - 6.89 (m, 4H, ArH), 5.82 (s, 1H, HC = C), 5.14 - 5.06 (m, 1H, CH), 2.99 (d, J = 13.7 Hz, 1H, CH2), 2.56 (d, J = 13.7 Hz, 1H, CH2), 2.48 - 2.30 (m, 2H, CH2), 2.26 (d, J = 11.2 Hz, 1H, CH2), 2.12 (s, 3H, CH3), 1.87 (s, 3H, CH3), 1.83 (d, J = 11.2, 5.5 Hz, 1H, CH2), 1.72 ? 1.62 (m, 1H, CH), 1.09 (s, 3H, CH3), 1.06 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 206.91, 191.53, 164.42, 161.86, 143.72, 135.89, 129.75, 126.96, 119.23, 117.68, 112.47, 75.08, 46.18, 42.24, 41.15, 31.58, 30.96, 30.08, 22.66, 22.41, 17.61, 10.97. HR-MS (ESI): Calcd. C22H26O4, [M+H]+ m/z: 354.1906, found: 354.1907.
1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl-4-chlorobenzoate (1g). Yellow solid, yield 63%, mp: 69.2˚C - 72.8˚C, 1H NMR (400 MHz, CDCl3) δ 7.98 - 7.43 (m, 4H, ArH), 5.81 (s, 1H, HC = C), 5.10 - 5.02 (m, 1H, CH), 2.98 (d, J = 13.7 Hz, 1H, CH2), 2.56 (d, J = 13.7, 1H, CH2), 2.44 - 2.30 (m, 2H, CH2), 2.25 (d, J = 11.1 Hz, 1H, CH2), 2.11 (s, 3H, CH3), 1.86 (s, 3H, CH3), 1.84 - 1.76 (m, 1H, CH), 1.58 (d, J = 11.2 Hz, 1H, CH2), 1.08 (s, 3H, CH3), 1.04 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 206.99, 191.61, 165.32, 164.83, 143.62, 139.52, 130.97, 128.77, 128.74, 127.04, 126.84, 74.65, 46.26, 42.26, 41.14, 31.59, 30.92, 30.07, 22.63, 22.15, 17.17, 10.86. HR-MS (ESI): Calcd. C22H25ClO3, [M+H]+ m/z: 372.1570, found: 372.1571.
1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl-2-methoxybenzoate (1h). White solid, yield 73%, mp: 102.1˚C - 103.7˚C, 1H NMR (400 MHz, CDCl3) δ 7.77 - 6.96 (m, 4H, ArH), 5.80 (s, 1H HC = C), 5.11 - 5.01 (m, 1H, CH), 3.91 (s, 3H, CH3), 2.97 (d, J = 13.7 Hz, 1H, CH2), 2.56 (d, J = 13.7, 1H, CH2), 2.45 - 2.31 (m, 2H, CH2), 2.24 (d, J = 11.0 Hz, 1H, CH2), 2.11 (s, 3H, CH3), 1.87 (s, 3H, CH3), 1.78 (d, J = 11.0 Hz, 1H, CH2), 1.62 - 1.51 (m, 1H, CH), 1.10 (s, 3H, CH3), 1.08 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 206.89, 191.67, 165.95, 165.27, 159.13, 143.36, 133.47, 131.31, 127.18, 126.70, 120.13, 112.09, 74.07, 55.90, 46.27, 42.29, 41.17, 31.61, 30.16, 22.59, 22.12, 17.19, 10.74. HR-MS (ESI): Calcd. C23H28O4, [M+H]+ m/z: 368.2071, found: 368.2072.
A mixture of petasin derivatives (1 equiv) which were synthesized as the route II and m-ClC6H5COOOH (1.5 equiv) in CH2Cl2 was stirred at room temperature for 3 h. The disappearance of petasin derivatives was monitored by TLC. Upon completion, the solvent was removed and water was added, the reaction mixture was extracted with CH2Cl2. The combined organic layer was washed with brine, dried over anhydrous MgSO4 and concentrated under vacuum to afford the crude product. The crude product was purified by a flash column to yield the pure product 2a-e.
3',3',8,8a-tetramethyl-3-oxo-3,5,6,7,8,8a-hexahydro-1H-spiro[naphthalene-2,2'-oxiran]-7-ylbenzoate (2a). White solid, 90%, mp: 136.1˚C - 137.6˚C, 1H NMR (400 MHz, CDCl3) δ 8.07 - 7.48 (m, 5H, ArH), 5.97 (s, 1H, HC = C), 5.14 (m, 1H, CH), 2.66 (d, J = 14.5 Hz, 1H, CH2), 2.54 - 2.45 (d, J = 14.5 Hz, 1H, CH2), 2.38 (d, J = 11.2 Hz, 1H, CH2), 2.28 - 2.15 (m, 2H, CH2), 1.89 (d, J = 11.2, 1H, CH2), 1.72 - 1.64 (m, 1H, CH), 1.48 (s, 3H, CH3), 1.33 (s, 3H, CH3), 1.30 (s, 3H, CH3), 1.05 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 194.74, 167.29, 166.14, 133.17, 130.13, 129.59, 129.59, 128.46, 128.46, 125.42, 73.47, 65.79, 65.15, 47.00, 42.12, 40.48, 31.47, 30.48, 21.20, 19.31, 18.93, 10.68. HR-MS (ESI): Calcd. C22H26O4, [M+H]+ m/z: 354.1925, found: 354.1924.
3',3',8,8a-tetramethyl-3-oxo-3,5,6,7,8,8a-hexahydro-1H-spiro[naphthalene-2,2'-oxiran]-7-yl-4-methoxybenzoate (2b). White solid, yield 92%, mp: 206.4˚C - 208.8˚C, 1H NMR (400 MHz, CDCl3) δ 8.02 - 6.95 (m, 4H, ArH), 5.96 (s, 1H, HC = C), 5.10 (m, 1H, CH), 3.89 (s, 3H, CH3), 2.66 (d, J = 14.5 Hz, 1H, CH2), 2.49 (d, J = 14.5 Hz, 1H, CH2), 2.40 - 2.33 (d, J = 11.2 Hz, 1H, CH2), 2.27 - 2.08 (m, 2H, CH2), 1.87 (d, J = 11.2 Hz, 1H, CH2), 1.76 - 1.68 (m, 1H, CH), 1.48 (s, 3H, CH3), 1.33 (s, 3H, CH3), 1.29 (s, 3H, CH3), 1.03 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 194.76, 167.47, 165.89, 163.55, 131.63, 131.63, 125.36, 122.50, 113.70, 113.70, 73.04, 65.81, 65.14, 55.48, 47.07, 42.13, 40.49, 31.55, 30.51, 21.19, 19.31, 18.93, 10.66. HR-MS (ESI): Calcd. C23H28O5, [M+H]+ m/z: 384.1995, found: 384.1996.
3',3',8,8a-tetramethyl-3-oxo-3,5,6,7,8,8a-hexahydro-1H-spiro[naphthalene-2,2'-oxiran]-7-yl-2-nitrobenzoate (2c). White solid, yield 91%, mp: 169.8˚C - 171.7˚C, 1H NMR (400 MHz, CDCl3) δ 7.97 - 7.68 (m, 4H, ArH), 5.96 (s, 1H, HC=C), 5.15 (m, 1H, CH), 2.63 (d, J = 14.8 Hz, 1H, CH2), 2.52 (d, J = 14.7 Hz, 1H, CH2), 2.47 (d, J = 11.2 Hz, 1H, CH2), 2.13 (m, 2H, CH2), 1.78 (d, J = 11.2 Hz, 1H, CH2), 1.70 - 1.63 (m, 1H, CH), 1.48 (s, 3H, CH3), 1.32 (s, 3H, CH3), 1.29 (s, 3H, CH3), 1.04 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 194.62, 166.79, 165.14, 147.90, 133.03, 131.82, 129.58, 127.77, 125.49, 124.01, 75.51, 65.72, 65.13, 46.56, 42.13, 40.36, 30.65, 30.30, 21.19, 19.27, 18.92, 10.59. HR-MS (ESI): Calcd. C22H25NO6 [M+H]+ m/z: 399.2785, found: 399.2783.
3',3',8,8a-tetramethyl-3-oxo-3,5,6,7,8,8a-hexahydro-1H-spiro[naphthalene-2,2'-oxiran]-7-yl-2-fluorobenzoate (2d). White solid, yield 87%, mp: 136.4˚C - 137.8˚C, 1H NMR (400 MHz, CDCl3) δ 7.86 - 7.32 (m, 4H, ArH), 5.97 (s, 1H HC = C), 5.16 - 5.05 (m, 1H, CH), 2.66 (d, J = 14.4 Hz, 1H, CH2), 2.46 (d, J = 14.4 Hz, 1H, CH2), 2.41 - 2.33 (d, J = 11.1 Hz, 1H, CH2), 2.26 - 2.11 (m, 2H, CH2), 1.89 (d, J = 11.2 Hz, 1H, CH2), 1.72 - 1.63 (m, 1H, CH), 1.48 (s, 3H, CH3), 1.33 (s, 3H, CH3), 1.30 (s, 3H, CH3), 1.04 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 194.68, 167.0, 164.99, 163.73, 161.27, 132.31, 130.17, 125.34, 120.14, 116.61, 74.01, 65.76, 65.16, 46.91, 42.11, 40.46, 31.39, 30.41, 21.19, 19.29, 18.93, 10.69. HR-MS (ESI): Calcd. C22H25FO4, [M+H]+ m/z: 372.1805, found: 372.1806.
3',3',8,8a-tetramethyl-3-oxo-3,5,6,7,8,8a-hexahydro-1H-spiro[naphthalene-2,2'-oxiran]-7-yl-2-fluorobenzoate (2e). White solid, yield 87%, mp: 123.8˚C - 124.1˚C, 1H NMR (400 MHz, CDCl3) δ 7.23 - 6.87 (m, 4H, ArH), 5.92 (s, 1H HC=C), 4.86 (m, 1H, CH), 3.82 (s, 3H, CH3), 3.58 (s, 2H, CH2), 2.53 (d, J = 14.4 Hz, 1H, CH2), 2.46 (d, J = 14.4 Hz, 1H, CH2), 2.23 - 2.11 (d, J = 11.2 Hz, 1H, CH2), 2.04 (m, 2H, CH2), 1.68 (d, J = 11.2 Hz, 1H, CH2), 1.54 - 1.47 (m, 1H, CH), 1.45 (s, 3H, CH3), 1.30 (s, 3H, CH3), 1.21 (s, 3H, CH3), 0.86 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 194.68, 171.53, 167.21, 158.76, 130.19, 130.19, 125.95, 125.35, 114.03, 114.03, 73.20, 65.74, 65.08, 55.26, 46.73, 42.02, 40.82, 40.39, 31.28, 30.37, 21.16, 19.21, 18.91, 10.37. HR-MS (ESI): Calcd. C24H30O5, [M+H]+ m/z: 398.2160, found: 398.2161.
A mixture of petsasinol (1 equiv) and Maleic anhydride (0.9 equiv) in CH2Cl2 in the presence of (C2H5)3N (1 equiv) was stirred at 0˚C for 2 h. The disappearance of compound petsasinol was monitored by TLC. Upon completion, the solvent was removed and water containing HCl was added, the reaction mixture was extracted with CH2Cl2. The combined organic layer was dried over anhydrous MgSO4 and concentrated under vacuum to afford the crude product. The crude product was added to a stirred solution of Excessive halids in the presence of NaCO3 in DMF at 65˚C. The disappearance of the crude product was monitored by TLC. Upon completion, the solvent was removed and water containing NaCl was added, the reaction mixture was extracted with EA. The combined organic layer was dried over anhydrous MgSO4 and concentrated under vacuum to afford the crude product. The crude product was purified by a flash column to yield the pure product 4a-b.
allyl (1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl)-fumarate (4a). Yellow oil, yield 68%, 1H NMR (400 MHz, CDCl3) δ 6.31 (d, J = 12.2 Hz, 1H, HC = C), 6.28 (d, J = 12.1 Hz, 1H, HC = C), 5.97 (m, 1H, HC = C), 5.80 (s, 1H, HC = C), 5.38 (d, J = 17.2 Hz, 1H, HC = C), 5.30 (d, J = 10.4 Hz, 1H, HC = C), 4.96 (m, 1H, CH), 4.71 (d, J = 5.8 Hz, 2H, CH2), 2.94 (d, J = 13.7 Hz, 1H, CH2), 2.49 (d, J = 13.6, 4.4 Hz, 1H, CH2), 2.34 (m, 2H, CH2), 2.21 (d, J = 11.2 Hz, 1H, CH2), 2.11 (s, 3H, CH3), 1.87 (s, 3H, CH3), 1.74 - 1.67 (d, J = 11.2 Hz, 1H, CH2), 1.51 (m, 1H, CH), 1.05 (s, 3H, CH3), 1.02 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 191.64, 164.89, 164.84, 163.42, 143.65, 131.53, 130.02, 129.58, 127.01, 126.76, 118.97, 75.03, 65.92, 45.99, 42.23, 41.10, 31.23, 29.99, 22.63, 22.14, 17.17, 10.70. HR-MS (ESI): Calcd. C22H28O5, [M+H]+ m/z: 372.2015, found: 372.2013.
1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl-propylfumarate (4b). Yellow oil, yield 69%, 1H NMR (400 MHz, CDCl3) δ 6.29 (d, J = 12.3 Hz, 1H, HC = C), 6.25 (d, J = 12.3 Hz, 1H, HC = C), 5.80 (s, 1H, HC = C), 4.95 (m, 1H, CH), 4.16 (t, J = 6.8 Hz, 2H, CH2), 2.94 (d, J = 13.7 Hz, 1H, CH2), 2.50 (d, J = 13.7 Hz, 1H, CH2), 2.41 - 2.27 (m, 2H, CH2), 2.21 (d, J = 11.1 Hz, 1H, CH2), 2.11 (s, 3H, CH3), 1.87 (s, 3H, CH3), 1.74 (dd, J = 14.3, 7.1 Hz, 2H, CH2), 1.69 - 1.64 (d, J = 11.1 Hz, 1H, CH2), 1.56 - 1.48 (m, 1H, CH), 1.05 (s, 3H, CH3), 1.02 (d, J = 6.7 Hz, 3H, CH3), 0.98 (t, J = 7.4 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 191.66, 171.60, 165.32, 164.88, 143.55, 130.10, 129.54, 127.04, 126.76, 74.91, 66.92, 51.64, 45.99, 42.22, 41.09, 31.23, 30.00, 22.62, 21.81, 20.72, 17.15, 10.70. HR-MS (ESI): Calcd. C22H30O5, [M+H]+ m/z: 374.2174, found: 374.2172.
A mixture of petasinol (1 equiv) and TsOCl (1.5 equiv) in CH2Cl2 in the presence of (C2H5)3N (1 equiv) was stirred at 0˚C for 6 h. Then the mixture was filtered and concentrated in vacuum. The residue was purified by flash column to yield the pure product 5. The NaN3 was added to a stirred solution of product 5 in DMF at 95˚C for 8 h. The disappearance of product 5 was monitored by TLC. After purification, the product 6 was afforded and was reduced with Zn power (1 equiv) in the presence of NH4Cl (2 equiv) to produce 7. Condensation of product 7 with different kinds of acyl chloride (2 equiv) in CH2Cl2 in the presence of (C2H5)3N (1 equiv) resulted in acyl derivatives. Upon completion, the solvent was removed and water was added, the reaction mixture was extracted with CH2Cl2. The combined organic layer was dried over anhydrous MgSO4 and concentrated under vacuum to afford the crude product. The crude product was purified by a flash column to yield the pure product 8a-c.
N-(1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl)acetamide (8a). Yellow oil, yield 66%, 1H NMR (400 MHz, CDCl3) δ 5.96 (s, 1H, NH), 5.75 (s, 1H, HC = C), 4.21 (m, 1H, CH), 2.87 (d, J = 14.4 Hz, 1H, CH2), 2.44 (d, J = 14.6, Hz, 1H, CH2), 2.24 - 2.18 (d, J = 11.1 Hz, 1H, CH2), 2.15 - 2.04 (m, 2H, CH2), 2.09 (s, 3H, CH3), 2.02 (s, 3H, CH3), 1.82 (s, 3H, CH3), 1.75 - 1.67 (d, J = 11.1 Hz, 1H, CH2), 1.24 (m, 1H, CH), 1.03 (s, 3H, CH3), 1.01 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 191.44, 169.98, 165.75, 144.53, 127.17, 127.11, 49.39, 43.82, 41.67, 41.03, 30.95, 27.18, 23.77, 22.80, 22.31, 18.18, 12.02. HR-MS (ESI): Calcd. C17H25NO2, [M+H]+ m/z: 275.1972, found: 275.1975.
2-chloro-N-(1,8a-dimethyl-6-oxo-7-(propan-2-ylidene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl)acetamide (8b). Yellow oil, yield 66%, 1H NMR (400 MHz, CDCl3) δ 6.98 (s, 1H, NH), 5.83 (s, 1H, HC = C), 4.20 (m, 1H, CH), 4.13 (s, 2H, CH2), 2.93 (d, J = 14.2 Hz, 1H, CH2), 2.47 (d, J = 14.2 Hz, 1H, CH2), 2.32 - 2.23 (d, J = 11.1 Hz, 1H, CH2), 2.18 - 2.03 (m, 2H, CH2),2.13 (s, 3H, CH3), 1.85 (s, 3H, CH3), 1.75 (d, J = 11.1 Hz, 1H, CH2), 1.26 (m, 1H, CH), 1.11 (s, 3H, CH3), 1.07 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 191.40, 165.75, 165.17, 144.61, 127.17, 127.06, 50.17, 43.76, 43.01, 41.58, 40.89, 30.75, 27.03, 22.78, 22.26, 17.88, 12.09. HR-MS (ESI): Calcd. C17H24ClNO2, [M+H]+ m/z: 309.1579, found: 309.1580.
4-chloro-N-(1,8a-dimethyl-6-oxo-7-(propan-2-yli-dene)-1,2,3,4,6,7,8,8a-octahydronaphthalen-2-yl)benzamide (8c). Yellow oil, yield 66%, 1H NMR (400 MHz, CDCl3) δ 7.71 - 7.45 (m, 4H, ArH), 6.33 (s, 1H, NH), 5.86 (s, 1H, HC = C), 4.43 (m, 1H, CH), 2.95 (d, J = 14.2 Hz, 1H, CH2), 2.49 (d, J = 14.2 Hz, 1H, CH2), 2.33 - 2.28 (d, J = 11.1 Hz,), 2.22 - 2.19 (m, 2H, CH2), 2.15 (s, 3H, CH3), 1.92 (d, J = 11.3 Hz,), 1.87 (s, 3H, CH3), 1.31 - 1.25 (m, 1H, CH), 1.17 (s, 3H, CH3), 1.13 (d, J = 6.7 Hz, 3H, CH3). 13C NMR (100 MHz, CDCl3) δ 191.37, 166.38, 165.29, 144.75, 137.92, 133.09, 131.46, 129.05, 128.77, 128.13, 127.24, 127.06, 50.12, 44.03, 41.59, 41.05, 30.89, 27.20, 22.83, 22.32, 18.29, 12.18. HR-MS (ESI): Calcd. C22H26ClNO2, [M+H]+ m/z: 371.1710, found: 371.1708.
The petasin derivatives were assayed for in vitro anticancer activity by using MTT method. Cells were seeded in 96-well plates at a density of 4.6 × 103 cells/well for 24 h. The cancer cell lines included SK-N-SH, MGC-803 and HepG-2. The seeded cells were treated with various concentrations of the test derivatives for 48 h. After 20 ml of MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) solution (5 mg/mL) was added to each well and incubated for 4 h at 37˚C. The medium containing MTT was discarded; then 150 mL of dimethyl sulfoxide (DMSO) was added to each well. And the plates were agitated until the dark blue crystals (formazan) had completely dissolved. The absorbance was measured using a microplate reader at a wavelength of 490 nm. The average 50% inhibitory concentration (IC50) was determined from the concentration response curves according to the inhibition ratio for each concentration.
Cell activity data were analysed by the SPSS 17.0 software and were expressed as means ± standard error (SE).
The authors are grateful for the financial support from the National Natural Science Foundation of China (No. 81273393).