Urinary bladder cancer is the second most common genitourinary malignancy in Westernized countries. A number of emerging imaging techniques have recently been reported in the literature for use in imaging bladder cancer. While the explicit application of MR imaging in bladder cancer especially in non-distant bladder cancer or bladder cancer localized in pelvic remains uncertain and awaits a thorough evaluation. Therefore, this article aims to make a systematic review of previous literature and provide a complete assessment of the value of MR as an evolving imaging tool in localized urinary bladder cancer application. Studies were conducted by searching the MEDLINE and PubMed databases up to 2012 using both medical subject heading (Mesh) and a free text method like “urinary bladder cancer”, “magnetic resonance imaging” or “MRI”, “diffusion weighted imaging” or “DWI”. About 53 articles were selected and analyzed which were considered to be associated with the objective of this systematic review (Table 1). Results showed that MRI especially the newer functional imaging technique like DWI may has a great potential role in localized bladder cancer like early detection, staging, evaluation of tumor aggressiveness and therapeutic responsiveness of patients in the assessment of urinary bladder cancer and so forth. Newer MRI has already proceeded promising results in bladder cancer application; and the explicit role of newer MRI in bladder cancer patients still needs further research and awaits to be thoroughly evaluated.
It is widely appreciated that urothelial tumors are the most common histopathological type and account for approximately 90% - 95% of all bladder tumors [1-3]. This leads one to assume that the diagnosis of bladder cancer is relatively easy or straightforward. However, this overly simplistic consideration, when one considers the multifactorial nature of the management of bladder cancer, actually goes well beyond the diagnosis of the tumor itself. This includes such complex factors as the staging of bladder tumors, the aggressiveness of the specific tumor type and perhaps even therapeutic response assessment of patients presenting with this disease. Further complicating these considerations is the realization of the multifocal nature of bladder tumor, the high recurrence rate, and a certain trend in disease progression. These features collectively convince us that both early screening and preoperative staging are beneficial if an optimal treatment plan is to be realized.
The preoperative estimation for tumor stage is a multifaceted process, requiring a combination of clinical and radiological assessments to objectively evaluate the extent of the tumor growth no matter organ-confined or nonorgan-confined. An accurate preoperative staging of disease is the key to assuring a correct treatment regimen, and in this regard, prognosis largely depends on clinical and radiological staging at presentation [
Pathologically, bladder cancers are staged using a TNM staging system. And according to the 2004 World Health Organization/International Society of Urological Pathology classification, a two-tiered grading system for bladder cancer was introduced, which categorized bladder tumors as low and high grade, additionally, it was classified as belonging to either the noninvasive or invasive type of tumor. Tumors with a high frequency and
“-” indicate that there’s no article on that aspect. “Systematic” represents that the article about bladder cancer MR imaging is a comprehensive article involve many aspects about bladder cancer. “Diagnosis/detection” represents that the article is mainly about the diagnosis or detection of bladder cancer. “Staging/grade” represents that the article is mainly about the bladder cancer staging or tumor grade. “Lymph nodes” indicates that the article is subjected to the lymphatic metastasis associated with bladder cancer. “Therapeutic evaluation” represents that the article is about the therapy response evaluation for bladder cancer patients with MR imaging. “Pathology” represents that the article is about the pathology of bladder cancer related to bladder cancer MR imaging. “others” indicates those articles do not subject to the content about DWI (diffusion weighted MR imaging) or DCE (dynamic contrast enhanced MR imaging).
exhibiting highly varied biologic behavior were classified as high-grade tumors, and those unlikely to present aggressive behavior were defined as low-grade tumors [
In general, surgical treatment of patients with early stage tumors like T1, or lower stage bladder tumors are achieved by an initial transurethral resection (TUR) procedure. Those presenting with a T2 or higher stage with organ-confined tumors usually undergo a partial or radical cystectomy, with or without adjuvant therapies [1,2].
While there’s a fact we have to admitted is that there’s a proportion of lower stage or non-muscle invasive bladder tumors remaining in a high grade state, and almost 50% of non-muscle invasive lesions will recur, among which, 30% will experience disease progression into muscle-invasive lesions [
However, there remains no reliable mean to predict which high-grade non-muscle invasive cancers will progress to an invasive form or even display subsequent metastases. If all patients with high-grade noninvasive tumors underwent the therapeutic options as mentioned above, we submit that it would highly likely constitute an over-treatment of those patients by exposing them to the undue additional risk of complications and associated toxicity. This would of course reduce the quality of patients’ life, especially for those who likely have no realistic expectation or potential for disease progression [
Moreover, it is important to mention that 20% - 25% of epithelial tumors are muscle invasive and are often of the high histologic grade [
Since the depth of tumor invasion, and estimation of metastatic condition especially for non-organ-confined bladder tumors cannot be reliably determined by cystoscopy, bladder imaging provides a vital tool in the disease management protocol. One of the most promising imaging tools that has been studied most recently is the highly developed and advanced magnetic resonance imaging (MRI) technique with a high magnetic field strength of 3.0 Tesla (3.0 T). Thus in the following sections, we aim to mainly discuss the current uses and potential role of MRI in the diagnosis and management of bladder tumors especially those non-distant metastatic lesions, with particular emphasis on the role provided by the 3.0 T MRI.
As compared other imaging techniques like ultrasound and CT, MRI has many advantages. These include no risk of radiation exposure and subsequent harmful effects, having an intrinsic high soft tissue resolution, and being able to provide multi-planar parameters and imaging capabilities, among others. Currently, the most commonly used scanners for MRI of the bladder are equipped with a 1.5 T magnetic scanner. Sequences are typically as follows: a large field of view (FOV) of T1-weighted (T1W) imaging on which bladder cancers often appears with an intermediate signal intensity similar to muscle tissue, which actually assists in the identification of infiltration of the perivesical fat by the tumor, lymphadenopathy and bone metastasis.
High resolution fast T2-weighted (T2W) imaging can be obtained with a small FOV and a large matrix on which the bladder cancer manifests as a low signal intensity, providing information on tumor depth and involvement of adjacent structures [13-16]. This is possible because the normal bladder muscle wall manifests itself one or two layers of low (low inner and high outer) signal intensity on T2WI images [
The application of diffusion weighted MR (DWI), with 3.0 T MR scanner in bladder cancer is a relatively new and evolving technique in recent years. The basic principal of DWI is that it quantifies the diffusion of water molecules in tissues. DWI images thus provide both
qualitative and quantitative information, which can be applied in the differentiation of benign from malignant tissue without implementing intravenous contrast material [20,21]. Currently, DWI images are usually obtained with a single-shot SE echo planar sequence performed with a chemical shift selective fat-suppression techniques with b values of 0 and 800, or 1000 sec/mm2, with diffusion gradients being applied in three orthogonal directions. Routinely, images are obtained in the axial planes, and sometimes other institutions will obtain images taken in the sagittal planes.
On DWI images, like other malignancies ,bladder tumor lesions often can be detected by visual assessment as a high-SI lesion on high-b-value images (e.g., 800 - 1000 sec/mm2) even it is small partly because the diffusion of water molecule is restricted, which results from greater cellularity, decrease of the extracellular space as well as the tissue organization distortion. This clearly demonstrates an obvious contrast with the low SI of urine in the full bladder and in terms of the surrounding fat. The bladder wall will usually present as an intermediate SI on high b-value images just as image (
MRI especially the recently emerging new DWI-MRI technique have been reported can have clinical utility at all stages of a bladder cancer patient’s journey from detection to diagnosis, for staging and assessing therapy
response, and finally, for assessing relapse, though questions still revolve around whether the reported results can be measured reliably and reproducibly and whether the results predict important clinical outcomes related to therapy [
1) T2WI imaging The current anatomical staging criteria commonly used for T2WI images are as follows: a lesion with intact low signal intensity (SI) muscle-layer at the base of the tumor, is diagnosed as T1 or less (NMIBC); a lesion with focally disrupted low signal muscle-layer without perivesical infiltration, is diagnosed as T2 (MIBC). In addition, a lesion extending into the perivesical fat (T3) or the adjacent organs (T4) is also diagnosed as MIBC [22-24].
On MRI sequence images, sub-mucosal linear enhancement (SLE) is depicted immediately after the injection of contrast agent, and the signal intensity of the muscle layer remains low. Therefore, an intact SLE adjacent to a tumor is thought to be indicative of stage T1 or lower. When the SLE is disrupted by a tumor in the absence of tumor infiltration into the perivesical fat, then stage T2 is considered. The criteria for considering stages T3 and T4 are remarkably similar to those shown for T2WI images [16,25,26].
Currently, most studies describing bladder cancer staging using DWI, applied the criteria first proposed by Takeuchi et al. proposed [
Using conventional MRI, previous studies have demonstrated a staging accuracy which ranged between 72% and 95%. However, these studies were performed with low field-strength scanners (0.5 Tesla) and contained a small sample size [22,24,28]. In addition, Buy et al. reported a staging accuracy of 60% for non-enhanced MR images [
The value of DCE-MRI in bladder cancer may have find clinical utility in evaluating enhancement patterns of bladder lesions [4,30]. However, this idea remains highly controversial. SLE is considered to be major signal marker for the differentiation of T1 or lower versus T2 or higher stages. However, at the current time, it would be premature to draw firm conclusions on whether or not the use of gadolinium (Gd)-enhanced MRI has a supplementary value in diagnosing bladder cancer. Others have shown that Gd-DTPA enhancement in MRI, improved the accuracy of cancer staging in intramural bladder cancer [
When combined with the DWI approach, a study by Abou-El-Ghar [
In addition, the measurement of ADC values from DW imaging is currently considered the optimal imaging method for in vivo quantification of the combined effects of capillary perfusion and water diffusion. Moreover, on the corresponding ADC maps, the bladder tumors display a low SI, which is in contrast to the high SI seen with urine. This approach enables delineation of the tumor and quantification by means of the ADC value, which is usually around 1 × 10−3 mm2/sec in a malignant tumor, if b values of 0 and 800 - 1000 sec/ mm2 are applied [24,27].
In general, the absolute ADC values for tumors are significantly lower than those found for peripheral structures, which is markedly indicative that a malignant lesion exists. However, some inconsistent results still exist according to several prior studies. For example, Matsuki et al. reported that there was no overlap between the ADC values of the tumors and those of the urine or bladder wall. However, overlap could be seen between tumors and the prostate or seminal vesicles [
The condition of regional lymph node metastasis is also an essential determinant of survival for patients, and this is despite effective local and systematic therapy [10-12]. Prior studies have shown that extended lymphadenectomy is substantiated by the fact that 33% of patients with unexpected microscopic nodal involvement at the time of cystectomy have metastatic lesions [
The diagnosis of benign or metastatic lymph nodes using conventional methods like CT or MRI are based on lymph node appearance such as size, contour and internal contrast enhancement, among other factors. Usually, pelvic nodes greater than 8 mm and abdominal nodes greater than 10 mm in the maximal short axis diameter are considered metastatic [43,44]. Despite these criteria, there remains a lack of both sensitivity and specificity, which often leads to a high false negative result. The major reason accounting for this is that small metastatic lymph node may be missed. Additionally, marginal locations of reactive enlarged benign lymph nodes are also often misdiagnosed. Although there is some evidence that conventional MRI is superior to CT scanning in detecting sub-centimeter lymph nodes, MRI still can not distinguish those lymph nodes with micro-metastatic disease [45,46].
Previously, the use of ferumoxtran-10-enhanced MR imaging was reported. Using this approach, there was significant improvement in nodal staging in patients with bladder cancer by detection of metastases even in normal-sized lymph nodes. In addition, the sensitivity and negative predictive values significantly improved nodal staging at post-contrast (from 76% to 96%) as compared with those at pre-contrast imaging (from 91% to 98%). However, the limitation associated with this approach is that the time period to complete the examination is extended to some degree [
While DWI as a functional MRI imaging technique, combining the measurement of ADC values, it represents a interesting field of research and this technique holds much promise for the future. A study that assessed the application of DWI in the detection of pelvic lymph node metastases in bladder cancer patients showed that the ADC value of metastatic lymph nodes was significantly lower than that of non-metastatic lymph nodes. In addition, the ADC value of 0.86 × 10−3 mm2/s provided the clearest separation between metastatic and non-metastatic lymph nodes according to the ROC curve described in this study [
However, although MRI has exhibited many advantages in the application of non-distant metastatic lesions for bladder cancer patients ,we cannot deny the limitation still exist given the distant metastatic lesions are unascertainable especially when the metastasis are diffused, on which, 18FDG-PET/CT is much more sensitive in those extrapelvic metastasis detection.
Conventional MRI has been rarely used in differentiating bladder cancer by histological grade. While the widespread application of functional DWI-MR in other organ systems like brain, breast, rectum, etc, has proven to be useful, it has also been consistently used recently in the examination of the urogenital system. Takeuchi et al. used the ADC map to generate a region of interest that was larger than 5 mm in bladder tumors to evaluate the degree of diffusion in the tumor. In this study, a correlation was made between the ADC values and histopathologic findings of the tumor, and this showed that the mean ADC value (b values 0 and 1000 sec/mm2) of G3 bladder tumors (0.81 ± 0.11 × 10−3 mm2/s) was significantly lower than that of G1 and G2 tumors. However, there was a significant overlap between G1 and G2 tumors, for which the ADC values were respectively measured as (1.29 ± 0.21) × 10−3 mm2/s, (1.13 ± 0.24) × 10−3 mm2/s, and one of the key reasons for this was that these studies referred to the 1973 WHO classification for tumor grading [27,50].
Additionally, Rosenkrantz reported on a study comprised of 17 patients with pathological confirmation of high-grade bladder cancers. This study showed that the ADC value was significantly lower among cases with metastatic disease than those in the absence of metastatic disease with an ADC value of 1.07 ± 0.18 × 10−3 mm2/s in the former and 1.45 ± 0.22 × 10−3 mm2/s in the latter. In addition, the sensitivity and specificity were 87.5% and 100% respectively, which suggested that ADC value may serve as a supplement parameter for predicting tumor behavior and making appropriate treatment decisions.
Although radical cystectomy is the reference standard in the management of organ-confined muscle-invasive bladder cancer, a proportion of patients undergoing bladdersparing transurethral resection supplemented with radiotherapy or chemotherapy, etc, may achieve a promising outcome and in time, an improvement in their quality of life [51-54]. Usually, only those patients who achieve a complete response after chemotherapy are selected for a bladder-sparing strategy, which is accompanied by additional radiotherapy, otherwise, a radical cystectomy has to be considered. Thus, an accurate treatment response evaluation and prediction of an optimal selection of patients suitable for a bladder-sparing protocol are more necessary [53,54].
We suggest that MRI is the most promising technique, and has the potential to assist in bladder-sparing protocols. Comparable to CT scanning, the conventional T2- WI and dynamic enhancement images still present difficulties in distinguishing the residual tumor tissues and the bladder wall thickening, as well as inflammation and fibrotic changes, especially after TUR therapy several weeks after cessation of therapy [55-57]. A recent studies attempting to evaluate the DWI application role in the follow-up of non-muscle invasive urinary bladder carcinoma after transurethral resection concluded that DWMRI has a high reliability in differentiating post-TUR inflammatory changes from bladder tumors recurrence, which is similar to that of cystoscopy [
Although most studies were conducted in relatively small cohorts of patients, the study results describing the application of MRI especially DWI, in non-distant metastatic bladder cancer diagnosis staging and prediction of tumor aggression, even in the context of therapeutic response evaluation and postresection follow up, were very promising. Therefore, future studies will likely include larger patient cohorts to fully validate the role of newly emerging MRI technique. This is especially true in terms of the use of DWI, which combining the quantitive measurement of the ADC value as a biomarker for accurate T staging and in the pretreatment of predicted tumor aggression, and perhaps also to evaluate the therapeutic response in bladder cancer patients.