Two species of radishes, Raphanus sativus and Raphanus raphanistrum, were grown in the laboratory in the same substrate consisting of a smectite-type clay, which was watered at the beginning of the experience with 50 ml of a solution containing either none or 1000, 2000 or 4000 mg/L of AgNO3, respectively. Occurrence of the toxic metal in the substrate outlines higher element uptakes by the cultivated species Raphanus sativus than by the wild species Raphanus raphanistrum, except for the highest degree of Ag pollution. After a one-month growth in the Ag-polluted substrate, Raphanus sativus was depleted in most of the major, trace and rare-earth elements, except for Al, Fe, Th, Ag and U that increased in the radishes from substrate polluted by 2000 mg/L of AgNO3, and Sr, Co, Ni, U and Ag that increased in the radishes from substrate polluted by 4000 mg/L of AgNO3. Raphanus raphanistrum was enriched in all elements except Si, Na, Rb and K in the polluted substrate. The uptake was monitored by a cation-exchange process in the rhy-zosphere between mineral particles and the watering solution in the presence of various enzymes with specific activities that induced a variable uptake with the REEs being even fractionated. These activities most probably depend on combined factors, such as the plant species, and the chemical and physical properties of the substrate. The results obtained here reveal also that accumulation of nutrient elements and others in the plants is not uniform at a given Ag pollution of the substrate and therefore at a given Ag contamination in the same plant species.
The main anthropogenic source of heavy metals is presently in the metallurgic activities that often produce punctual elevated concentrations relative to the natural background [
Toxic ions present in soils can affect plants by damaging their root cells, which may lead to an inhibition in the nutrient transport from soil to root cells. A first-order consequence of such modifications is the reduction of the biomass that may also modify the production of either oxidizing or reducing agents, which, in turn, influences also the transfer of nutrients from soils to plants. Among the most studied heavy metals that damage soils are Pb, Cd and Ag. Anthropogenic sources of Ag, which impact on plants will be studied here, include Cu, Zn, Sn and Pb smelters, sewage sludges. It contributes also heavily to toxic waste of the photographic and imaging industry, and of electronic and electrical applications [
Despite extensive studies on element uptake and transport in plants, little attention has been paid to their fractionation in bioaccumulation processes. The available interpretations are also often based on investigations of plant-soil systems under open-field conditions. Even if the results of such studies provide useful information, the reactions between soils, microorganisms and plants are at times difficult to decrypt because of the complex composition and structure of the soils that do not allow unequivocal interpretations of the interactions, therefore not being comparable straight to results obtained under other open-field conditions in other soils, and/or under other climates.
In a recent study, the element uptake by spinach was quantified during a three-month growth in an illite substrate under strictly controlled laboratory conditions [
By carrying out an experiment on element transfers from a variably polluted, well-identified and homogeneous substrate under controlled laboratory conditions, the assigned goal of the present study is the control of the impact of the soil mineralogy on the mass transfers. The choice of pure smectite clay without specific contents of microorganisms as substrate, instead of a generic soil, was dictated by its high capacity of ion exchange with its immediate environment. The purpose was deliberately to avoid the impact of variations in water supply, soil texture, pH, temperature, humidity and availability on the transfer of essential nutrients to plants growing in highly polluted substrates.
In summary, the aim of this study is an identification of how plants take up chemical elements released by the mono-mineral substrate in a situation of extreme metal pollution, by focusing strictly on mineral-to-plant elemental transfers. We had not in mind to make an evaluation of the environmental relevance of the experiment as the type of substrate and the amount of Ag contamination were chosen to be far from natural conditions. Ultimately, the study was also not designed and performed to address biological and metabolic aspects of plants growing on natural soils in outdoor conditions, but to provide some understanding on how two different species of plants select the nutrient and other elements from an extremely Ag-contaminated mono-mineralic substrate.
Two species of radish were selected for the experiment: the cultivated species Raphanus sativus (called R. sativus hereafter) and the wild species Raphanus raphanistrum (called R. raphanistrum hereafter). The Raphanus gender was selected because it is a fast growing plant, allowing a rapid turnover in the experiment, and the choice of a wild and a cultivated species was decided to identify possible differences in their behavior relative to Ag contamination. The substrate consists of a sedimentary smectite-type clay mineral that is known for its specific high capacity of exchanging elements, such as Na, Ca or Mg and H2O molecules, temporarily bond in an interlayer position [
Thirty days after seeding, the plants were in a reasonably good shape and were harvested for chemical analysis. To constrain best the analytical uncertainty of the results, the following procedure was designed and applied: 1) the plants were grown in strictly the same conditions; 2) two containers of either no or the varied pollution degrees (1000, 2000 and 4000 mg/L of AgNO3) for each species (R. sativus and R. raphanistrum) were systematically taken into account: 30 - 35 individual plants were harvested from each container; 3) each batch of 30 - 35 plants from each container was analyzed independently; and 4) the results of the two same batches were averaged. The chemical compositions of the plants reported in the table represent, therefore, analytical averages of the data from about 60 - 70 individual plants collected independently at the end of the growth experiment in two batches from two containers.
The collected plants were first washed five times with deionized water. The washing was followed by a mild ultrasonic treatment in a bath for about 10 minutes to remove any solid mineral particle that could still adhere at the surface of the plants after the washing, especially at the roots. After the ultrasonic treatment, the plants were washed again with deionized water. The roots of some of the plants were observed by optical and scanning electron microscopy for any visible presence of mineral particles, which was never the case. Alternatively, it cannot be ignored that an ultrasonic treatment potentially removes the chemical elements adsorbed at the root surfaces. The two approaches having advantages and drawbacks, the choice was purposely in favor of cleanness and analytical reproducibility, that is to say in favor of the gentle ultrasonic treatment. We considered the fact to lose some information by removing the adsorbed elements from root surfaces to be less deteriorated than measuring the potential contribution of unexpected mineral particles. Afterwards, each set of plant samples was prepared for chemical determinations.
The plants were dried at 60˚C for 24 hours and weighed systematically on the same weight in the same temperature conditions. Then, they were ashed in a Pt crucible at about 600˚C for 45 min. The ash was transferred into a Teflon© beaker and digested in ultrapure concentrated HNO3 at a temperature of about 70˚C for 24 hours at least. The solution was then slowly evaporated to dryness by closing the beaker. Ten drops of HClO4 were added afterwards to ensure the complete dissolution of any remaining organic matter, and the aliquot was evaporated again to dryness. The solution for analysis was prepared by dissolving the dried material in a known volume of 1N HNO3.
The elemental contents of the plants were determined using an ICP-AES (JY124 model from Jobin Yvon Instruments S.A.) for the major elements and some trace elements (Sr and Ba), and an ICP-MS (XSeriesII model from Thermo Electron Corporation) with a collision chamber for the other trace elements, including the rareearth elements (REEs). Weakly analysis of the Gl-O and BE-N geostandards [27,28] were carried out during the course of the study for calculation of the analytical precision that is of ±2%, ±5% and ±10% for the major, trace and REEs, respectively, with a 2σ internal standard deviation. The individual analytical uncertainty was controlled as precisely as possible by duplicating the whole analytical procedure in two independent batches of plants and averaging them. It shall be remembered that the results were not biased by “soil heterogeneities” as the substrate consisted, on purpose, of a homogeneous claymineral type. Alternatively, we have postulated that any heterogeneity of the plants was “smoothened” by the high number of individuals (60 - 70) taken in two separate batches for each species at each pollution degree. Of course, possible manipulation errors cannot be excluded, but as they are difficult to quantify as such, we have tried to address that aspect by analyzing a high number of plant individuals. As the overall individual uncertainty is at the level of the analytical uncertainty, it can be assumed that no detectable manipulation mistake occurred during the experiment.
After 30 days of growth, the height of the radish plants was about 20 cm and the diameter of the leaves about 2 cm. Comparison between density and size of the plants in each pot showed that addition (called pollution hereafter) of 4000 mg/L Ag to the substrate caused a reduction of the number of plants and of the size of the leaves relative to the plants collected from unpolluted or less polluted substrate. Metal toxicity may having varied impacts on plants that depend on the amounts incorporated by the plants (called contamination hereafter), and therefore on their ability to adjust to the potential toxicity created by the abnormal content of Ag, the toxic metal. A routine evaluation of a metal contamination is the quantification of the metal added to the soil, if known, that gives its pollution degree. However, the possibility of a heterogeneous distribution of the metal contamination in the soil, even in an homogeneous substrate such as that used here, has been envisaged and we have also based the contamination on the amount of Ag incorporated by the plants, assuming that a higher Ag uptake (=contamination) corresponds to a higher Ag content in the root environment.
On the basis of this approach of the contamination scale, marked differences for the two plant species are visible (
Addition of 50 ml of a solution containing various amounts of Ag to the substrate showed that R. sativus took up less major elements relative to the same species grown in the unpolluted substrate with the lowest uptake for the lowest Ag contamination, except for Al and Fe, which contents increased when the plants were grown in the polluted substrate (
In the case of R. raphanistrum, the amounts of Si, Na and K taken up by the plants are systematically less when Ag pollution increased, therefore when contamination increased. The other elements were taken up more (
In comparing the amounts of the major elements picked up by both species, the wild species R. raphanistrum is systematically taking up less elements than the cultivated R. sativus, the difference being as much as 16.7% when grown in the substrate polluted by 2000 mg/L AgNO3.
R. sativus took up systematically less V, Cr, Pb, Rb and Sr, and more Cu and Ag, when the Ag pollution increased in the substrate (
The total concentration of REEs increased systematically in R. sativus grown in the polluted substrate. The REE content was almost 12 times more in the plants grown in the substrate polluted by 2000 mg/L AgNO3 than in the plants grown in the unpolluted substrate. At the highest AgNO3 pollution of 4000 mg/L, the REE contents were lower by about 34%. Normalization of the REE contents of R. sativus collected from each substrate, relative to those of the unpolluted smectite substrate, provide a depleted content in the middle REEs (MREEs) with a positive anomaly in Eu (
The distribution patterns of the REEs of R. raphanistrum grown in the polluted substrates are similar to those of R. sativus (
Before discussing the changes of the element uptakes by the two species of radish, it is probably appropriate to reiterate here the objectives of this experiment. The goal is the examination of the behavior of two plant species
growing in a homogeneous mono-mineralic clay-type substrate extremely polluted by Ag. The study was designed to focus on the initial mineral-to-plant elemental transfers depending on the degree of Ag pollution of the substrate, and not to address biological and metabolic aspects of plants growing on natural soils. Providing some understanding on how two different plant species select the nutrient elements from an Ag-polluted monomineralic substrate was also among the topics of interest.
The mobility of nutrients from soils to plants generally depends on the selective pick up by the plants, which in turn depends on the pH and solubility of the soil minerals, and more importantly on the activity of the microorganisms that may contribute to the mineral dissolution and the enzymes to the element transfers. These transfers were evaluated here for two species of plants that grew in the same substrate polluted by increasing amounts of AgNO3 by the soil solution, but depleted in microorgan-
isms during the experiment on the basis of the results by Semhi et al. [
as much as 4000 mg/L of AgNO3, R. sativus incorporated lower amounts of the elements, except Ca which amounts remained unchanged. For R. raphanistrum, the taking up of most elements increased in the presence of as much as 4000 mg/L of AgNO3, except Si, Na and K.
The amounts of most major and trace elements including the REEs decreased in R. sativus grown in the less polluted substrate (1000 mg/L AgNO3) compared to the same species grown in the unpolluted substrate. For R. raphanistrum grown in the same polluted substrate, the contents of most elements increased relative to those in the plants from the unpolluted substrate; Si, K, Na and Rb decreased. The uptake of the elements by the plants from less polluted substrate (1000 mg/L AgNO3) depends on the species, one taking up more and the other less. At the intermediate pollution concentration of 2000 mg/L of AgNO3, the content of most major and trace elements decreased in R. sativus, except for the increasing Al, Fe, Th, Ag and U, and for the unchanged Mg, Co,
V, Nb, Zn and the total REE contents. In R. raphanistrum, the content of most elements increased relative to the same species collected from unpolluted substrate, except for the decreasing K, Na, Si and Rb. Again the uptake appears to be species dependent. After addition of the high Ag pollution (4000 mg/L of AgNO3) to the substrate, the contents of most major and trace elements decreased in R. sativus, except for the unchanged Ca, Ba, Cu and Cd, and for the increasing Sr, Co, Ni, U and Ag relative to the species from the unpolluted substrate. For R. raphanistrum collected from 4000 mg/L AgNO3 polluted substrate, the contents of most elements increased relative to those in the same species from unpolluted substrate, except the decreasing Si, K, Na and Rb.
The response to an increasing Ag pollution obviously depends on the plant species, R. sativus picking up higher amounts of Ag at the two lower contamination concentrations than R. raphanistrum, which picked up more Ag at the highest pollution concentration (4000 mg/L of AgNO3). The highest enrichment in Ag in R. sativus was observed in the plants collected from substrate polluted with 2000 mg/L of AgNO3 and the highest enrichment in Ag in R. raphanistrum was observed in the plants collected from substrate polluted with two times more Ag (4000 mg/L). This difference in the accumulation of Ag by two species reflects a variable impact of Ag on their rhizosphere.
One of the most abundant elements in soils is Si that has here in the clay substrate an antagonistic behavior with Al and Fe in R. sativus from substrate polluted by 2000 mg/L of AgNO3. The higher Al accumulation in the plants induces a decrease in the mobility of Si, and a correlative increase in that of Fe and Al relative to the same elements in plants collected from other substrates. The content in Fe increased by about 82% and that in Al by about 165% relative to the same species grown in the unpolluted substrate. These changes correlate to higher Ag uptake by the plants. As for R. sativus, the highest mobility of Al and Fe from substrate to the plants was found when the substrate was polluted by 2000 mg/L AgNO3, whereas the highest Ag accumulation in R. raphanistrum was observed at a 4000 mg/L AgNO3 pollution.
The bioavailability of Al and Fe in the rhizosphere is influenced by the ionic species and concentrations, which both depend on pH and chemical composition of root exudates [
Since heavy metals such as Ag are picked up from soils and transported to cells via plant-nutrient transporters [
The high accumulation of Fe and Al in the plants grown during this study, either beneficial or toxic, is probably due to their high content in the clay substrate. Neither R. sativus nor R. raphanistrum collected from substrate polluted by 2000 mg/L of AgNO3 were characterized by an enrichment in Fe and Al relative to the same species grown in the unpolluted substrate. This important availability of Fe and Al may result from a variation in the biomass and the microbial processes induced by variations in pH, which in turn could have been induced by increased root exudation of organic acids [
The plant selectivity causing element fractionations can be evaluated by comparing elements that are chemically similar. In the present case, we have focused on changes of the Ca/Sr and K/Rb ratios, as well as on the REE distribution (
The concentrations of K and Rb decreased in R. sativus grown in the smectite substrates polluted by Ag relative to the same species from unpolluted substrate. An important increase of the K/Rb ratio was found for the plants collected from substrate polluted by 2000 mg/L of AgNO3, the uptake of Rb being reduced relative to that of K when the species incorporated more Ag.
In R. raphanistrum grown in all substrates, it can be seen that, although the decrease in either Rb or K, the K/Rb ratio increased in the plants grown in the substrates polluted by 2000 and 4000 mg/L of AgNO3, that is to say that the ratio increased in the plants when Ag increased in the substrate, because of a selective uptake of K relative to Rb. The mobility of both K and Rb was reduced due to a higher presence of Ag in the substrate, but this reduction is more important for Rb than for K, as it was for R. sativus. The K/Rb ratio remained unchanged in the plants from substrate polluted by 1000 mg/L of AgNO3, where the reduction of the K mobility was similar to that of the Rb. There is no selectivity in the uptake of K and Rb at this level of pollution. Variations in the ratio only occurred in the plants grown in the highly polluted substrate, which confirms the impact of contaminants on the enzymes and the fixation sites of the elements.
In R. sativus grown in the substrate polluted by 1000 mg/L of AgNO3, the contents of both Sr and Ca decreased at the same rate relative to the species grown in the unpolluted substrate, leaving the Sr/Ca ratio unchanged. In the same plants from substrate polluted by 2000 mg/L of AgNO3 that were contaminated the most by Ag, the mobility of Sr was reduced relative to that of Ca, while the Sr/Ca ratio remained unchanged again.
In R. sativus grown in the substrate polluted by 4000 mg/L AgNO3, the Sr concentration increased while the Ca concentration remained unchanged, which increased slightly the Sr/Ca ratio. In R. raphanistrum collected from variably polluted substrate, the contents of either Sr or Ca increased when Ag contamination in the plants increased, with more mobility for Sr than for Ca, inducing an increase in the Sr/Ca ratios.
As for elements with similar behaviors, potential clues of plant-soil interactions can be derived from examination of both the REE contents and distribution in plants or parts of plants, relative to the substrate normalized distribution of each lanthanide, e.g., by Semhi et al. [
Here, the collected plants have REE distribution patterns relative to their substrate that are characterized by: 1) a more pronounced enrichment in the LREEs than in the heavy REEs (HREEs), 2) a slightly negative Ce anomaly, and 3) a significant positive Eu anomaly. Such fractionations were attributed to variations in the stability constants of complexation processes involving an interaction of the REEs with different ligands. The literature abounds with studies on REE fractionation due to bindings to different common ligands such as carbonates, sulphates, phosphates, acetates, oxalates, etc. The readers may be referred to the works of Evans [
R. sativus collected from substrates polluted with 1000 and 4000 mg/L of AgNO3 was depleted in its overall contents in REEs relative to that grown in the unpolluted substrate. The REE transfer to the plants collected from substrate polluted with the intermediate Ag concentration of 2000 mg/L, that is to say to the plants that accommodated the highest absolute Ag concentrations measured in the plants (
All plants have a positive Eu anomaly relative to the REE distribution in the substrate, except for R. raphanistrum grown in the unpolluted substrate. This anomaly can reflect the Eu oxidation from a II(+) state to a III(+) state in the rhizosphere and its complexation. In both cases, the Eu anomaly increases in the plants grown in polluted substrates relative to those grown in the unpolluted substrate. It varies as follows: lowest in the plants from the substrate polluted by 1000 mg/L AgNO3, higher in plants from the substrate polluted by 4000 mg/L AgNO3, and highest in the plants from the substrate polluted by 2000 mg/L. Compared to the other REEs, the preferential transfer of Eu from substrates to both species of plants appears to be the highest at a substrate pollution level of 2000 mg/L, confirming that Ag addition to soils may not be necessarily toxic or even lethal to plants.
In a soil environment, the Eu anomaly is commonly explained in terms of changes in the oxidation-reduction conditions, since Eu can occur in natural mineral environments in both the Eu(II) and Eu(III) oxidation states. Based on this reasoning, the positive Eu anomaly observed here for the plants implies reduction conditions in the substrate environment favoring incorporation of Eu in the Eu(II) oxidation state. Since the roots of the plants were shallowly buried, only within 2 - 3 centimeters below the substrate surface, and the substrate on which the seeds were spread was only loosely packed, it is difficult to assert modifications in the oxidation/reduction conditions in the substrate during the period of plant growth. Also, since both species of radish had the same anomaly in Eu (about 1.6) relative to the substrate, which was lower at a pollution level of 1000 and 2000 mg/L of AgNO3, the anomaly may reflect the high availability of Eu in the substrate compared to the other REEs as well as its mobility from rhizosphere to the roots through preferential active sites on enzymes, which could have been modified by the abnormal concentrations of Ag.
The mechanisms by which Eu is enriched could record a modification in the enzyme activity in conjunction with a modification in the microorganic populations. These populations could have been altered by the addition of Ag to the substrate consequently inducing modifications in the oxidation-reduction conditions of the root environment. Such a hypothesis is supported by a previous study showing a Gd fractionation relative to the other REEs reported by Semhi et al. [
The increase in the REE contents of both species from polluted substrates relative to the same species from unpolluted substrate may result from a progressive increase in the amount of microorganisms and ligands involved in the complexation of the REEs that facilitate their transfer from rhizosphere to the plants. In fact, the fractionation of the REEs did not change significantly when the amount of Ag added to the substrates increased. Only the total amount of REEs transferred to the plants and the degree of the Eu anomaly changed. This could mean that the amount of polluting Ag was not determining for the uptake of REEs, as long as the amount of Ag in the substrate was significant. The mobility of REEs in the plants may not have changed, and this may be due to the resistance of some microorganism populations to the increasing Ag contamination. In this case, the enrichment in LREEs in both species could result from high concentrations of low-molecular organic ligands, the production of which being induced by the presence of Ag in the clay substrate.
Variable additions of AgNO3 as a pollutant to a substrate consisting of a smectite clay mineral that is interlayer expandable and cation exchangeable had varied impacts on the chemical composition of two species of radish grown during a laboratory experiment. The highest Ag uptake was by the cultivated Raphanus sativus, the highest “contamination” in the plant occurred at the addition of 2000 mg/L AgNO3 to the substrate and, surprisingly, the lowest plant contamination occurred at an addition of the highest AgNO3 pollution at 4000 mg/L. The Ag uptake by the wild Raphanus raphanistrum correlates positively with the increased addition of Ag, from 1000 to 4000 mg/L AgNO3, to the substrate. The pollution of the substrate by AgNO3 decreased the accumulation of most major and trace elements in the cultivated Raphanus sativus, while it increased their accumulation in the wild Raphanus raphanistrum.
The transfer of REEs from clay substrate to the two species of radish was increased by the addition of AgNO3 as a pollutant, whatever the amount. The highest uptake of REEs occurs at an intermediate addition of AgNO3 (2000 mg/L). The observed positive Eu anomaly in the REE patterns of the plants could have resulted from site activity of the enzymes or from microorganisms in the rhizosphere; it cannot be related to changing oxidationreduction conditions in the substrate during plant growth. The pollution of the substrate by AgNO3 did not induce a significant fractionation of the REEs in both radish species, when compared to the REE spectra of the species grown in the unpolluted substrate.
The experiment showed also that the use of a monomineralic substrate has the advantage to relate strictly the elemental variations in the plants to the microbiological activity after seeding, as almost none existed in the substrate before and to the enzymatic activity in the plant. It also allows discarding external parameters such as the physical and chemical properties of the soil and the climatic changes. Also, because of the abnormally high occurrence of AgNO3 in the substrate, changes in the elemental complexation and modification of enzyme activity could have occurred, potentially explaining the selective uptake of some elements and the changes in the element mobility in the two species of radish.
The authors are very grateful to Professor Mary Beth Kirkham (KSU, USA) for her detailed reading and improvement of the script before submission. We would also like to thank the two anonymous reviewers for the constructive comments and the editor of GSC for help during the publication round, as well as Hugh Rollinson and Tabisam Khan from the College of Sciences (SQU, Oman) for providing access to their laboratory for sample preparation.