Fraction

Total protein^a (mg)

Total activity^a (arbitrary units)

Specific activity (units/mg)

Yield^a (%)

Purification (-fold)

100,000 × g supernatant

12

820

68

100

1.0

DEAE-cellulose

3.7

250

68

30

1.0

Phenyl-sepharose

0.91

72

79

8.8

1.2

Hydroxyapatite

0.18

33

180

4.0

2.6