Targeted Macromolecules Delivery by Large Lipidic Nanovesicles Electrofusion with Mammalian Cells531
was reported 20 years ago [20]. The spontaneous desta-
bilization of the lipid bilayer of LUVs remains to be ex-
plained [21]. The field pulse induces a membrane desta-
bilization which is prone to a spontaneous fusion as pro-
posed by Rosenheck. Our results may be considered
within the framework of elucidating the physical basis of
exocytosis. The conclusion is that electropermeabiliza-
tion (and associated fusion) may be endogenously pre-
sent as already reported for peptide membrane permeabi-
lization [22]. A key feature of our delivery method is that
the transfer is obtained after a very short contact period
(10 min in this report), a major advantage for drug deliv-
ery. A final advantage is that the electrical conditions can
be used in clinical applications [23].
5. Acknowledgements
Supports from the region Midi Pyrénées, ANR Cemirbio,
PCV CNRS, ARC and Erasmus programs should be ac-
knowledged.
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