Vol.2, No.4, 383-391 (2011)
doi:10.4236/as.2011.24050
C
opyright © 2011 SciRes. Openly accessible at http://www.scirp.org/journal/AS/
Agricultural Scienc es
Effects of the inhibition of weed communities by
winter-flooding
Korehisa Kaneko1*, Toshihiko Nakamura2
1Hokuso Creature Associati on, Tabata, Tokyo, Japan; *Corresponding Author: k_kaneko@hotmail.com
2Natural History Museum and Institute, Chiba, Japan.
Received 9 September 2011; revised 14 October 2011; accepted 26 October 2011.
ABSTRACT
Winter-flooded paddy field is an agricultural
method which putting a water among the winter,
it is paid attention as an environmental friendly
agriculture. Especially, it is said, winter-flooding
is control paddy weeds and there is the farming
potential that the annual rice yield is high.
However, there is no detailed research about the
effects of weed communities by winter-flooding,
as the purpo se of this stu dy, we investigat ed the
effects of the inhibition of weed communities
(life cycle, harmful weed) by winter-flooding at a
shore of Inba Lake, Chiba Prefecture, Japan,
and compared them to paddy weeds in a dry
paddy field. Methods examined the plant height
(cm) and the cover degree class of all appear-
ance species in each quadrant frame (1 m2).
Quadrant in the two type paddies were 10
frames (D1-D10) at the control, and were 10
frames (W1-W10) at the treatment (Figure 1 ). In a
winter-flooded paddy field, species number and
plant volume of winter-annual plants decreased
in the third winter after winter-flooding, In par-
ticular, the plant v olume of Alopecuru s aequalis,
Cardamine flexuosa decreased significantly. We
considered that the cause of this decrease was
due to the depth of flooding (more than 10 cm).
Eleocharis kuroguwai and Sagittaria trifolia,
which are perennial plants, Echinochloa ory-
zoides, w hich is an annual plant, increased in a
winter-flooded paddy field in the third summer
after winter-flooding. We considered that these
species grew thicker at places where the seeds
germinated, and grew easily, because the con-
servation situation of the seeds fitted well under
the winter-flooding conditions. They started to
grow immediately after the drainage of water.
The period of drainage coincided with increas-
ing light intensity and temperature. In addition,
the annual rice yield of the third year after win-
ter-flooding was higher than that of the habitual
practice rice field
Keywords: Winter-Flooded Paddy Field; Life Type;
Harmful Weeds; Biodiversity; Environmental
Agriculture
1. INTRODUCTION
In Basic Law on Food, Agriculture and Rural Areas
which enacted in 1999, it is requested the sustainable
demonstration of multi-functionality, not only the stable
supply of food, but also the conservation of country and
water-source, the formation of natural environment and
excellently landscape etc through th e productive activity.
Afterwards, original biodiversity strategy is enacted in [1]
and [2], environmental friendly agriculture has been
contained as o ne of the bi o diversity conse rv at ion.
In revision of Ramsar convention in 2005, rice paddy
fields were registered, in particular, winter-flooded rice
farming which is conducted in Kabukuri-numa and the
surrounding rice paddies is attracting attention as an en-
vironment to be worthy not only the safety and healthy
rice but also the respect of biodiversity as used land of
many waterfowls.
In winter-flooding, the paddy of non-irrigation period
is the flooding state, as the effects, the water-source
conservation in winter and water qu ality purification [3],
the offer of biotope to wetland including waterfowl etc.
[4], the effects of agricultural management of accompa-
nying additional value as the effects of the weed sup-
pression and environmental preservation rice [5-7]. [8]
reported that the effects of the weed suppression found
in the second winter after winter-flooding. However,
there is no research example of the detailed examination
as the effects that the winter-flooding exerts on life cycle
(annual plant, winter-annual plant and perennial plant) of
paddy weeds and harmful weeds. These are the problems
which should be examined to promote the winter-flood-
K. Kaneko et al. / Agricultural Sciences 2 (2011) 383-391
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384
ing in the future.
This study aimed to clarify the effects of the inhibition
of weed communities by winter-flooding, comparing to
plant communities between before winter-flooding (the
first year) and the third year after winter-flooding (the
finality year), based on a vegetation survey of the win-
ter-flooding examination.
2. MATERIALS AND METHODS
2.1. Study Site
Study site is suited at a large-scale rice field of the
Hagiyama Shinden section in a shore of Inba lake in
Sakura City, Chiba Prefecture, Japan.
Paddy field in this area has produced Koshihikari.
Field experiments were set as the treatment where the
winter-flooding was conducted and the control where the
habitual practice rice farm was continued. In the first
year of winter-flooding, water put in January 2006,
scratched and planted rice was conducted in May, the
herbicide was scattered in June, the drainage of water
was conducted in the middle of August, rice was har-
vested in the middle of September. The rice bran was
scattered in October, water was flooded in the beginning
of November. In the second year, the scratching, the
planting rice, the drainage of water, the harvesting rice,
the flooding were almost conducted at same periods in
the first year, the herbicide was not scattered. However,
the herbicide scattered in the third June.
The mean water depth was about 10 cm at the first
and the second year, and was about 12 cm in the third
year. The winter-flooding was supplied at constant in-
tervals using a pump. The scratching, the planting rice,
the drainage of water, the herbicide scatter conducted at
the same time on the control of dry paddy and the treat-
ment before winter-flooding.
2.2. Vegetation Survey
The vegetation survey was conducted in August, Oc-
tober, and December which are both the first year and
the third year, and methods examined the plant height
(cm) and the cover degree class of all appearance species
in each quadrant frame (1 m2).
Quadrant in the two type paddies were 10 frames
(D1-D10) at the control, and were 10 frames (W1-W10)
at the treatment (Figure 1).
Inba lake, Sakura city (Quotation from Inba laka HP)
W1
W2
W3 W4
W5 W6
W7
W8
W9
W10
D1
D2
D3 D4
D5 D6
D7
D8
D9
D10
水 路
冬期湛水水田
慣行水田
N
100m50m
0m
Treatment
(Winter-flooded paddy)
100×90m
2
Control
(Dry paddy)
100×90m
2
Winter-Flooded paddy
Dry paddy
Inba Lake
Waterway
佐倉市
千葉県
Sakura city
Chiba Prefecture
Japan
Figure 1. Investigation position.
K. Kaneko et al. / Agricultural Sciences 2 (2011) 383-391
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385385
2.3. Analysis
The plant volume of each species (=the average rate
(%) × the plant height (cm)) calculated as an index of the
plant biomass. The average rate converted each cover
degree class (+: <1%, 1: 1% - 5%, 2: 5% - 25%, 3: 25% -
50%, 4: 50% - 75%, 5: 75% - 100%) into 0.1%, 2.5%,
15%, 37.5%, 62.5%, and 87.5%, based on [9].
In addition, in order to examine the changes of the
composition and the structure of plant communities in
the two type paddies, the cover degree (+, 1, 2, 3, 4, 5)
of appearance species in the quadrant frame of each sec-
tion (+ class converted into 0.1 and the following class
was not converted) were analyzed by cluster analysis,
using the group average method (PC-ORD: Windows
version 4.01).
As for the life circle’s definition, the annual plant is a
plant that germinates the seed, flowers, brings forth
within one year, leaves the seed, and withers. The win-
ter-annual plant is a plant that the seed germinates in
autumn, passes the winter at rosette, it will flower and
bring forth next year. The perennial plant is a plant that it
is growing as an individual for two or more years.
3. RESULTS
3.1. Species Number in before and after
Winter-Flooding
Compared to species number between the treatment
and the control in before winter-flooding (2005) and the
third year after winter-flooding (2008), although all
founded species did not have a significant difference
with before winter-flooding, and were significant differ-
ence (5%) in the third December, species number was
little at the treatment. This result has seen the same ten-
dency with wi nt er-ann ual pl an t (Fi gure 2).
051015 0510 15
Des
Oct
Aug
051015 0510 15
Des
Oct
Aug
051015
0510 15
Des
Oct
Aug
n.s.
n.s. n.s.
n.s.
**
n.s.
n.s
n.s
n.s
n.s
*
**
n.s
n.s
Control
Treatment
Annual
Winter-annual
Total
Before (2005)Third year (2008)
051015
n.s
**
n.
s
051015
Des
Oct
Aug n.s
n.s
*
Perennial
n.
s
Mean number of plant species(/m
2
)
Dec
Dec
Dec
Dec
Figure 2. Mean number of plant species of total and each life type in treatment (winter-flooded
paddy) and control (dry paddy). **: 1% significant, *: 5% significant, n.s.: non-significant.
Horizontal stick shows mean value of standard error margin. Significant used t-authorization of
student. N = 10 shows sample number of each quadrant.
K. Kaneko et al. / Agricultural Sciences 2 (2011) 383-391
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386
3.2. Plant Volume in before and after
Winter-Flooding
Compared to plant volume between the treatment and
the control in before winter-flooding (2005) and the third
year after winter-flooding (2008), plant volume were
little at the treatment in the third December, a significant
difference was at 1%. This was the same tendency with
winter-annual pl ant s ( Figure 3).
010002000300040005000 01000 2000 3000 4000 5000
Des
Oct
Aug
010002000300040005000
01000 2000 3000 4000 5000
Des
Oct
Aug
010002000300040005000 01000 2000 3000 4000 5000
Des
Oct
Aug
n.s.
n.s.
n.s
n.s.
n.s.
**
n.s.
n.s.
n.s.
n.s.
n.s.
n.s.
n.s.
**
**
010002000300040005000
01000 2000 3000 4000 5000
Des
Oct
Aug n.s.
n.s.
n.s.
n.s.
n.s.
n.s.
Before (2005)Third year (2008)
Annual
Winter-annual
Total
Perennial
Control
Treatment
Mean volume of plant species(/m
2
)
Dec
Dec
Dec
Dec
Figure 3. Mean volume (/m2) of plant species of total and each life type in treatment (winter-flooded paddy) and control (dry paddy).
**: 1% significant, *: 5% significant, n.s.: non-significant. Horizontal stick shows mean value of standard error margin. Significant
used t-authorization of student. N = 10 shows sample number of each quadrant.
K. Kaneko et al. / Agricultural Sciences 2 (2011) 383-391
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387387
As the main paddy weeds wh ich influenced the changes
of the plant volume in the above-mentioned, an annual
plant was Echinochloa oryzoides, the winter-annual
plants were Alopecurus aequalis, Cardamine flexuosa,
the perennial plants were Eleocharis kuroguwai, Sagit-
taria trifolia. Compared to mean plant volume between
the treatment and the control, Echinochloa oryzoides
was hardly founded in before winter-flooding, however,
it was high at the treatment in the third summer . Alopecurus
aequalis, Cardamine flexuosa were high in the winter of
before winter-flooding, however, were low at the treat-
ment of the third year. Eleocharis kuroguwai, Sagittaria
trifolia were hardly founded in before winter-flooding,
however, they were extremely high at the treatment in
the third summer (Figures 4 and 5).
3.3. Species Composition in before and
after Winter-Flooding
Cluster analysis was conducted in order to examine
the changes of the composition and the structure in weed
communities in before winter-flooding and the third year
(Figure 6). It analyzed species composition according to
August, October, and December. As a result, the separa-
tion by clusters between the treatment and the control
were not confirmed in before winter-flooding, however,
in the third year, the treatment was separated with the
control except for W10 of October, W3 of December,
and was non-vegetation at 5 points (W1, W2, W7, W9,
W10).
4. DISCUSSION
As for the affects on weed communities by winter-
flooding, species number and plant volume of winter-
annual plants decreased in the treatment of the third De-
cember, especially, Alopecurus aequalis, Cardamine flexu-
osa greatly decreased. Although Alopecurus aequalis
germinate under conditions in which the temperature is
high, and the oxygen is satisfactory after the drainage of
water, germination is delayed in ill-drained paddy fields
and those th at are non-tilled with high groundwater lev el,
and will be carried over until spring of the following
year. The longevity of the seed is short from half a year
to a year, and the semination ability is low. The number
of seeds which dropped in the farmland provides for the
amount of generation in the farmland of the next genera-
tion [10] .
0100200300400500 0100 200 300 400 500
Des
Oct
Aug
0100200300400500 0100 200 300 400 500
Des
Oct
Aug
Alopecurus aequalis
(Winter-annual)
Cardamine flexuosa
(Winter-annual)
Before (2005)Third year (2008)
Control
Treatment
Mean volume of plant species(/m
2
)
Dec
Dec
Figure 4. Mean volume (/m2) of plant species which decreased by winter-flooded.
K. Kaneko et al. / Agricultural Sciences 2 (2011) 383-391
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388
050010001500 05001000 1500
Des
Oct
Aug
050010001500 05001000 1500
Des
Oct
Aug
Eleocharis kuroguwai
(perennial)
Echinochloa oryzoides
(perennial)
050010001500 05001000 1500
Des
Oct
Aug
Sagittaria trifolia
(annual)
Before (2005)Third year (2008)
Control
Treatment
Mean volume of plant species(/m
2
)
Dec
Dec
Dec
Echinochloa oryzoides
Sagittaria trifolia
Figure 5. Mean volume (/m2) of plant species which increased by winter-flooded. +: means not showing on the drawing because of
extremely the value low.
Moreover, [11] reported that Alopecurus aequalis
withered in a paddy field where the flooding was main-
tained enough in winter, and was generated in a paddy
field where the flooding was not maintained, and a final
amount of generation tripled. [12] reported that the gen-
eration rates of Alopecurus aequalis and Cardamine
flexuosa in a moist soil state were h igher than those in a
flooding water depth of 5 cm.
We considered that the germination and growth of
these species were inhibited by the flooding water depth
of 12 cm in autumn that winter-annual plants germinate,
and it greatly had an effect on the difference of species
composition between the treatment and the control in
winter.
K. Kaneko et al. / Agricultural Sciences 2 (2011) 383-391
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389389
Aug
※Treatment (W1~W2),Control (D1~D10)
Oct
Des
Quadrants where is no vegetation.
W1
W2
W7
W9
W10
Before (2005)Third year (2008)
D2
D8
D3
D7
D10
D4
D6
D9
D5
D1
W9
W3
W2
W4
W8
W7
W6
W5
W1
W10
4.4E-02 2.5E+014.9E+017.3E+01 9.7E+01
Distance(Objective Function)
100 7550250
Information Remaining(%)
D8
D3
D9
D6
D5
D2
D10
D7
D4
D1
W3
W4
W5
W6
W9
07.7E+00 1.5E+01 2.3E+01 3.1E+01
Distance(Objective Function)
100 7550250
Information Remaining(%)
W1
D1
D4
D9
D7
D8
W5
D3
W6
D2
W9
W10
D5
D10
W3
W7
W4
W8
D6
W2
1.5E-02 1.5E+012.9E+01 4.4E+01 5.8E+01
Distance(Objective Function)
100 7550250
Information Remaining(%)
D1
D2
W3
W4
D3
D10
W1
W5
W2
W6
W7
W9
W10
W8
D7
D4
D5
D8
D9
D6
01.4E+01 2.8E+01 4.3E+01 5.7E+01
Distance(Objective Function)
1007550 250
Information Remaining(%)
D1
D9
D5
D7
W3
D4
D8
D10
W1
W9
D3
D6
D2
W4
W2
W5
W6
W8
W10
W7
4.7E-03 1.4E+002.9E+00 4.3E+00 5.7E+00
Distance(Objective Function)
1007550 250
Information Remaining(%)
D1
W4
D7
D2
D3
D5
D4
D10
D9
D8
W9
W10
W5
D6
W7
W2
W8
W1
W3
W6
5E-031.7E+00 3.3E+015E+01 6.6E+01
Distance(Objective Function)
100 7550250
Information Remaining(%)
Dec
Figure 6. Difference of vegetation of each quadrant in treatment (winter-flooded paddy) and control (dry paddy).
[8] reported that Monochoria vaginalis of the annual
plants grew thicker at the treatment in the second sum-
mer. However, Monochoria vaginalis of the third year
did not grow thicker in comparison to that of the second
year, but instead, Echinochloa oryzoides of the annual
plants and Eleocharis kuroguwai, Sagittaria trifolia
K. Kaneko et al. / Agricultural Sciences 2 (2011) 383-391
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390
of the perennial plants grew thicker in summer of the
third year. Although a lot of seeds of Alopecurus ae-
qualis die out in dry paddy fields during the cryogenic
period from late autumn to the early spring, and the seed
survival time of Alopecurus aequalis in the soil is short,
in case of the paddy in the flooding state during year, the
extinction of the seeds in the process of the dormancy
awakening is hardly generated, the seed longevity are
longer compared with the dry paddy [13]. Moreover, the
population growth rate, the leaf area, and the net assimi-
lation rate of Alopecurus aequalis are high under the
conditions in which the quantity of solar radiation and
the temperature are high [14]. The sprout rate of tuber of
Eleocharis kuroguwai is high in flooded paddy fields of
paddling and under the strong reduction soil of non-
tillage, however, they are low under the conditions of
low temperature and drying treatment [15]. The germi-
nation rate of Eleocharis kuroguwai is high under flood-
ing condition s , and is low under drying conditions [16].
The production rate of the seed was high under the
conditions of strong light and eutrophication, and water
depth was growing excellently by 3 - 15 cm. In the case
in which these conditions exceeded the limit, it became
the life circle only vegetative propagation system, and
the tuber died out considerably by tillage an d g erminated
gradually after paddling [17]. We considered that the
seeds of Echinochloa oryzoides, Sagittaria trifolia, Eleo-
charis kuroguwai were in an excellent conservation state
by the winter-flooding, after the drainage of water, in
case that it became the state under the conditions in
which direct sunshine was strong and temperature was
high, the seed germinated and grew. Especially, in the
third year, Eleocharis kuroguwai and Sagittaria trifolia
grew remarkably thicker than Monochoria vaginalis. As
one of the agricultural factors related to the decrease of
annual plants and the succession to the perennial plant
communities such as Eleocharis kuroguwai and Sagit-
taria trifolia, a decrease of hand weeding by herbicide
scatter was the cause [18]. Therefore, we considered that
the use of the herbicide influenced the decrease of
Monochoria vaginalis in the third year. As other causes,
we considered that the tuber of Eleocharis kuroguwai
and Sagittaria trifo lia which germinated and grew in the
second year had increased after the winter-flooding, and
the vegetative propagation was actively done and grew
thicker in the third year, because the treatment plowed a
field lightly.
Although the volume of annual rice yield was high at
the control before winter-flooding, in the third year, it
was high at the treatment, therefore, we can be said that
there is little influence due to a mat of weeds in summer.
However, [19] reported that the production rate of rice
decreased in winter-flooded paddy fields (the flooding
began in the midd le of December) in which p addy weeds
grew thicker. This result is different from this research
study results. We have considered the beginning period
of winter-flooding and climate influences, and that they
are problems to be solved in the future.
5. ACKNOWLEDGEMENTS
This research conducted as a part of Mitamesi winter-flooding pro-
ject and Mitamesi action of “The Committee for Lake Inba-numa Wa-
tershed Management”. As for advancing the research, we are very
indebted to Dr. Katumi Musiake chaiman, Chiba Prefecture River
Environment Division, other parties concerned, and, Mr. Masuo Mi-
kado who cooperated in this research to offer the rice field while mak-
ing rice. Moreover, as for writing this paper, we got an offer of the
document material and valuable advice from Dr. Takuya Mineta of
National Institute for Rural Engineering, Dr. Hiroshi Jinguji who is
Associate Professor of Miyagi University. We wish sincerely to express
our gratitude to everybody.
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