The Effects of Auxins and Cytokinin on Growth and Development of
(Musa sp.) Var. “Yangambi” Explants in Tissue Culture
2179
highest root length of 3.69 cm was achieved under 1.0
mg/l IBA for excised shoots of banana [29]. This may be
due to genotype of their cultivar and the relatively low
concentration of IBA used, given that the experiment was
conducted for six weeks.
In our study, it was evident that 2.0 mg/l can increase
the average root length within short time and reduce the
costs associated with tissue culture. This may have a
positive influence on the survival of the explants.
4. Conclusions
The optimum concentration of BAP for buds prolifera-
tion of this variety (Yangambi) was 6 mg/l as reflected
by increased number of buds and fresh weight. The use
of BAP in combination with IAA (BAP * IAA) was
found to enhance fresh weight at concentration of 6 mg/l:
0.35 mg/l respectively. Therefore, due to the genotype
specificity on response of many banana cultivars to tissue
culture media, it is recommended that this concentration
be used for in vitro propagation of this variety.
As for rooting, supplementing a media for tissue
culture of banana variety “Yangambi” with 2.0 mg/l of
IBA increased the number and length of roots formed
and reduce the time required to wait for roots to be
formed in basal media or media with activated charcoal.
5. Acknowledgements
This study was funded by the Nelson Mandela African
Institute of Science and Technology through research
funds from Commission for Science and Technology
(COSTECH) in Tanzania.
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