Food and Nutrition Sciences
Vol. 2 No. 6 (2011) , Article ID: 6625 , 12 pages DOI:10.4236/fns.2011.26091
Quality Characteristics of Gamma Irradiated Beefburger Formulated with Partial Replacement of Beef Fat with Olive Oil and Wheat Bran Fibers
1Atomic Energy Authority, National Center for Radiation Research and Technology, Cairo, Egypt; 2Atomic Energy Authority, Nuclear Research Center, Abou Zaabal, Egypt.
Received January 21st, 2011; revised July 15th, 2011; accepted July 23rd, 2011.
Keywords: Irradiation, beefburger, low-fat, microbiological quality, olive oil, wheat bran
Five beefburger formulations were prepared with substituting beef fat with olive oil and levels of wheat bran and irradiated at doses of 0 and 3 kGy, then samples were refri-gerated stored and their quality characteristics were investigated. The results indicated that replacement of beef fat with olive oil and ascending levels of wheat bran in burger batter significantly decreased their contents of total lipids and saturated fatty acids, while increased their contents of dietary fibers, unsaturated fatty acids and the ratios of unsaturated and polyunsaturated fatty acids to the saturated ones. Moreover, significant improvements were observed in the cooking yield and moisture and fat retention of samples, proportionally to the added wheat bran, and both raw and cooked burger samples showed a high sensory acceptability. Irradiation of samples effectively inactivated Staphylococcus aureus, Salmonella and enterobacteriaceae and significantly reduced the counts of mesophilic and psychrophilic bacteria as well as molds and yeasts without any adverse effects on the quality characteristics of samples. Thus, reducing beef fat levels with the addition of olive oil and wheat bran produced a highly acceptable beefburger products with improved nutritional content as well as improved cooking and binding properties, while irradiation improved their microbiological quality.
Food and its manufacture are currently attracting significant scientific and public interest due to extensive media coverage of diet-related diseases and their influence on the health and wellbeing of communities. Recent advances in food and nutrition sciences have highlighted that it is possible to help optimize certain physiological functions through the diet and/or dietary components in order to improve health status and wellbeing and/or reduce the risk of disease. Like other food-related sectors, the meat industry is undergoing major transformations, driven among other things by changes in consumer demands . Meat and meat products are seen to be a major source of fat in the diet and provide high amounts of saturated fatty acids and cholesterol, which have been implicated in diseases include various cancers, obesity, hypertension, cardiovascular diseases, and coronary heart diseases [2-5]. A focus of dietary recommendations for cardiovascular disease prevention and treatment has been a reduction of saturated fat intake .
However, Fats in meat products play important role in stabilizing meat emulsions, reducing cooking loss, improving water holding capacity, providing juiciness and hardness and have considerable effects on the binding, rheological and structural properties of the meat product [7-9]. Fat reduction can, therefore, increase the toughness of meat products and significantly alter their acceptability [10,11]. The incorporation of vegetable oils in meat products to replace animal fat may have a positive effect on consumer health as they are free of cholesterol and have a higher ratio of unsaturated to saturated fatty acids. Moreover, different fiber-rich ingredients have recently been used as a functional additives to numerous ground and emulsified meat products to support and ensure binding. The reduction of animal fat in meat products and the substitution of animal fat with vegetable oils and dietary fibers could result in healthier products [12-14].
Of vegetable oils, olive is the one that has received most attention, chiefly as a source of monounsaturated fatty acids. It has a high biological value and contains antioxidant substances in optimum concentrations. Olive oil intake is associated with a lessened risk of heart disease and breast cancer and has positive effects on colon cancer [15-18]. In addition, interest in dietary fiber is a consequence of the belief that it contributes positively to the consumer’s health and quality of life due to its physiological effects .wheat bran is the most popular source of dietary fiber, with widespread recommendations for prevention and treatment of constipation and diabetes and reducing the risk of certain types of cancer [20-23]. It forms a good source of protein and minerals, in addition to its high fiber content and antioxidant activity [24,25].
On the other hand, meat products constitute a major source for pathogens that cause foodborne illness in human and awareness of risks involving their microbial contamination showed a significant continuous increase. Food irradiation is proven to be the best technology in eliminating disease-causing pathogens from raw meat products [26,27]. Gamma irradiation at dose of 3 kGy can be applied for improving the microbiological safety of raw meat products . Therefore, The objective of this study was to evaluate the quality characteristics of gamma irradiated beefburger formulated by partial replacement of the beef fat with different levels of olive oil and wheat bran fibers.
2. Materials and Methods
Fresh lean beef and beef fat were excised from three beef carcasses at the butcher’s shop (after three hours of slaughtering and dressing) to be used for the preparation of burger formulations in three separate trials (3 different replicates). The lean beef cuts were trimmed off any subcutaneous or intramuscular fats as well as thick or connective tissue, being a very lean beef meat. Then the lean beef and beef fat (within each of the different replicates) were ground separately in a meat grinder. Whole wheat bran was obtained from Al-Sharkia Milling Company, Zagazig, Egypt. Defatted soy flour was obtained from the Agricultural Research Center, Ministry of Agriculture, Egypt. Olive oil and the other ingredients of high quality were obtained from a local market.
2.2. Beefburger Manufacturing
Five different beefburger formulations (F1-F5) were prepared within each trail replicate (Table 1). The control beefburger formula (F1) consisted of ground lean beef, ground beef fat, soy flour, salt, Sodium tripolyphosphate, dreied onion, spice mixture (black pepper and cumin), and water (within the percentages described by Feiner, ). Since a very lean beef cuts with an expected low fat content were used for burger formulation, ground beef fat was added at level of 200 g/kg meat batter in the control burger formulation. Then the other burger formulations (F2-F5) were prepared by partial replacement of the beef fat with different percentages of olive oil and wheat bran as shown in Table 1. The ingredients of each of the formulated burger batter were thoroughly mixed by hand and processed into burgers of about 100 g weight and 10 cm diameter. The prepared burgers were aerobically packaged (individually) in polyethylene pouches and pouches were sealed by heat.
Table 1. Beefburger formulations with varying levels of beef fat, olive oil, and wheat bran.
The observed burgers were divided into two main portions and immediately transported in an ice chest for irradiation treatment.
2.3. Irradiation of Samples
Packaged samples of the prepared beefburger were exposed to gamma irradiation at doses of 0 and 3 kGy. Irradiation was carried out at room temperature using an experimental Co-60 source (providing a dose rate of 2.527 kGy/h) located at the National Center for Radiation Research and Technology, Nasr City, Cairo, Egypt.
2.4. Storage and Sampling
The irradiated and non-irradiated beefburger samples were refrigerated stored at 4˚C ± 1˚C (except samples for the day zero analysis) and subjected to the periodical analysis at 3 days intervals.
2.5. Proximate Composition
Moisture, protein, ash and dietary fibers were determined according to AOAC official methods , while total lipids were extracted and determined according to the method of Folch et al. . The remaining of other carbohydrates were calculated by difference.
2.6. Fatty Acid Profiles
Fatty acids of the extracted lipids were converted to their methyl esters  and the analysis of fatty acid methyl esters (FAME) was accomplished using a 6890 Hewlett Packard gas chromatograph equipped with flam ionization detector. The peak areas and retention times were measured using a Hewlett Packard 3392A integrator. Identification of FAME was based on comparing their relative retention times with those of reference ones.
2.7. Cooking Yield, Moisture Retention and Fat Retention
Samples of beefburger were cooked in a preheated electrical grill for 4 min each side, then cooking yield and fat retention were calculated according to Murphy et al. , while moisture retention was determined according to El-Magoli et al.  using the following equations:
Cooking yield% = (Cooked burger weight)/(Uncooked burger weight) × 100
Moisture retention% = (% yield × % moisture in cooked burger)/100
Fat retention% = [(Cooked weight) × (% fat in cooked burger)/(Raw weigt) × (% fat in the raw burger)] × 100
2.8. Measurement of Oxidation
The extent of oxidation in raw beefburger samples was assessed through the determination of thiobarbituric acid reactive substances (TBARS) using the method of Alasnier et al.  and the results were expressed as mg malonaldhyde per kg burger meat.
2.9. Microbiological Quality
At time of withdrawal from refrigerated storage, 10 g aliquots of burger samples were removed aseptically to prepare the initial 1/10 dilution which was used for the preparation of other serial dilutions in 0.1% peptone water. Then colony forming units for total aerobic mesophelic and psychrophilic bacteria were determined by plating on plate count agar medium and incubation at 30˚C for 3 days and 7˚C for 7 days, respectively . Total molds and yeasts were enumerated on malt agar medium after incubation at 25˚C for 3 - 5 days . Enterobacteriaceae were counted on violet red bile glucose agar medium after incubation at 37˚C for 20 - 24 h . Staphylococcus aureus was counted using Baird-Parker RPF medium after incubation at 35˚C for 24 - 48 h  and confirmed by the coagulase test as described by Collins et al. . The detection of Salmonella was carried out using most probable number technique (MPN) according to ISO . The samples were inoculated into buffer peptone for 24 h at 37˚C. After enrichment at 37˚C for 24 h in selenite broth, the cultures were streaked on brilliant green agar and incubated at 37˚C for 24 h, then biochemical examination in triple sugar iron agar (TSI) and lysine decarboxilase broth was conducted.
2.10. Sensory Evaluation
Raw samples of irradiated and non irradiated beefburger were subjected to sensory evaluation for their color & appearance and odor post treatment and during refrigerated storage, while the cooked beefburger samples were evaluated for their appearance and color, odor, taste and texture and juiciness on day zero only for safety precautions. In all sensory evaluation tests, the panelists consisted of ten non expert members from our laboratory, while scores were obtained by rating the examined quality attributes using the following rating scale: 9—excellent, 8—very good, 7—good, 6—below good/ above fair, 5—fair, 4—below fair/above poor, 3—poor, 2—very poor and 1—extremely poor. Rating of 5 and above indicated an acceptable samples, while rating of 4 indicated that the samples were of marginal quality, whereas rating of 3 and below indicated unacceptable samples .
2.11. Statistical Analysis
All analyses were performed using three pouches per each separate replicate. Then data were statistically analyzed by using the generalized linear model procedure of the SAS software , and the differences among means (at p < 0.05) were compared by using Duncan's multiple range test.
3. Results and Discussion
3.1. Proximate Composition
The results of compositional analysis for the different formulated beefburger samples are given in Table 2. The percentages of moisture, protein, total lipids, ash, dietary fibers, and carbohydrates in the non-irradiated control burger formula (F1) were 58.76%, 15.67%, 20.20%, 2.72%, 1.11%, and 1.54%, respectively. Incorporation of wheat bran into meat batter with the reduction of the added beef fat significantly increased the contents of total fibers and reduced the contents of total lipids for the resultant beefburger formulations, proportionally to the added bran fibers. The total lipids decreased by 20%, 42%, 53%, and 64%, while the dietary fibers increased by 206%, 412%, 514%, and 618%, respectively, in the different formulated burger samples (F2-F5) as compared with the control burger formulation (F1). Much attention has been recently paid to develop meat products with physiological functions to promote health conditions and prevent the risk of diseases . Increased intake of dietary fibers has been recommended for reducing the risk of colon cancer, diabetes, obesity and cardiovascular diseases in human [43-45].The same results clearly indicate that non of the determined macronutrients for all formulated burger samples showed a significant (P > 0.05) changes due to gamma irradiation at dose of 3 kGy (Table 2).
3.2. Fatty Acid Profiles
Table 3 represents the fatty acid profiles of lipid separated from non-irradiated and irradiated beefburger under investigation. Lipids of all non-irradiated burger formulations contained the same fatty acids. However, lipids of the control burger formula (F1) had the highest content of saturated fatty acids (SFA) reaching 51.6% of the total fatty acids and the predominant SFA were palmitic, stearic, and myristic, respectively. Meanwhile, the total unsaturated fatty acids (UFA) reached 46.05% and oleic was the most abundant monounsaturated fatty acid, while linoleic and linolenic were the predominant polyunsaturated fatty acids. Similar results were reported for lipids of beef patties . Incorporation of olive oil into the beef batter with decreasing of the added beef fat significantly (P < 0.05) decreased the contents of SFA in lipids of the different formulated burger samples accompanied with a significant increase of their USFA content, proportionally to the reduction of the added beef fat. Accordingly, a significant increases (P < 0.05) in the ratios of unsaturated/saturated and polyunsaturated/ saturated fatty acids were observed, indicating an improvement of the burger nutritional content (Table 3). Similar observations were reported for the replacement of the animal fat with vegetable oils in different meat products [12,47]. Healthier lipid formulation based on processing strategies is one of the most important current approaches to the development of potential meat-based functional foods . It was shown that low-fat, monounsaturated-rich diet reduced the susceptibility of low density lipoprotein peroxidation and may be of therapeutic value in the treatment of hypercholesterolemia . From Table 3, it can be also seen that lipids separated from the irradiated burger samples showed the same phenomenon and trends for their fatty acids similar to that observed for the non-irradiated samples. However, irradiation of the different formulated burger samples induced slight, but sta-
Table 2. Proximate composition of raw non-irradiated and irradiated (3 kGy) beefburger formulated with different levels of beef fat, olive oil, and wheat bran.
Table 3. Fatty acid profiles of lipids separated from different formulated non-irradiated and irradiated (3 kGy) beefburger.
tistically significant (P < 0.05), increase in the contents of SFA with corresponding significant decrease in the total UFA in their lipids. Similar results were previously observed .
3.3. Cooking Yield, Moisture Retention and Fat Retention
The measurements of cooking yield, moisture and fat retention in irradiated and non-irradiated beefburger samples as influenced by formulation with olive oil and wheat bran are summarized in Table 4. Formulation of beefburger with replacement of beef fat with olive oil and wheat bran significantly (P < 0.05) improved the cooking yield of samples as well as their moisture and fat retention. The observed improvement was pronounced with increasing the added bran fibers, as shown for the different formulated non-irradiated burger samples. The highest cooking loss was observed for the control formulated burger (F1) which may be attributed to the excessive fat separation and water release during cooking. The high ability of wheat fibers, as all other dietary fibers, to keep moisture and fat in the meat matrix of meat products is well documented and these observations are supported by several authors who used different types of fibers in the formulations of burger and other meat products [12,50,51]. Furthermore, cooking yield is influenced by the amount and type of fat in the meat product [52,53]. Reducing the animal fat content by replacement with vegetable oil increased the cooking yield of meat products . Neither irradiation at 3 kGy nor refrigerated storage (4˚C ± 1˚C) could significantly (P > 0.05) affect cooking and binding properties of the different formulated beefburger samples under investigation. As shown, no significant differences (P > 0.05) were observed between the measurements of cooking yield, moisture retention and fat retention for irradiated and
Table 4. Cooking yield, moisture retention and fat retention during refrigerated storage of non-irradiated and irradiated (3 kGy) beefburger as affected by formulation with olive oil and wheat bran.
non-irradiated beefburger samples post treatment and during refrigerated storage at 4˚C ± 1 ˚C (Table 4).
3.4. Extent of Oxidation
The development of oxidation during refrigerated storage (4˚C ± 1˚C) of the different formulated irradiated and non-irradiated beefburger samples was assessed through the measurement of TBARS values (Table 5). On day zero, the different formulated non-irradiated beefburger samples had an initial TBARS values of 0.23, 0.19, 0.17, 0.14 and 0.13 mg malonaldehyde /kg burger meat, respectively, showing the highest value for the control formulated burger (F1). The oxidative reactions are enhanced in meat products after mincing and restructuring as well as during refrigerated storage due to the interaction of unsaturated fatty acids with prooxidants . Irradiation of the different formulated beefburger samples at 3 kGy dose significantly (P < 0.05) increased their TBARS and the control formulated burger samples showed also the highest value. Moreover, subsequent refrigerated storage of the different formulated beefburger samples significantly (P < 0.05) increased their TBARS values for both irradiated and non-irradiated samples showing a higher values in the irradiated samples (Table 5). The oxidation of lipids due to irradiation and aerobic storage of meat products was previously reported by several authors [27,56]. As can be seen, the extent of oxidation significantly decreased with increaseing the added wheat bran in both non-irradiated and irradiated burger samples and also post irradiation and during storage, indicating the presence of enhanced antioxidant activity with increasing of the added bran fibers. The observed antioxidant activity was also apparent during the formulation of the burger batter as indicated by the significant decrease in the initial TBARS values with increasing of the added wheat bran for the different
Table 5. TBARS values for raw non-irradiated and irradiated (3 kGy) beefburger formulated with different levels of beef fat, olive oil, and wheat bran.
non-irradiated burger samples on day zero (Table 5). The observed antioxidant activity may be attributed to the added wheat bran fibers. It has been illustrated that wheat bran contains high total phenolic contents with high radical scavenging capability and antioxidant activity . Furthermore, olive oil, virgin and refined, contains also phenolic compounds with high antioxidant power .
3.5. Microbiological Quality
The results in Table 6 reveal a high initial microbial contamination in addition to the presence of Staphylococcus aureus and Salmonella in all formulated non-irradiated burger samples. It is well established that meats and meat products contain a broad spectrum of microorganisms and constitute a major source for foodborne pathogens . Irradiation of all formulated beefburger samples at dose of 3 kGy appeared to be effective in improving the microbiological quality of samples. The chosen dose effectively destroyed the present Staphylococcus aureus and Salmonella, which were not detected in the irradiated samples post irradiation and during their refrigerated storage. This is in addition to the observed significant reduction in the counts of mesophelic and psychrophilic bacteria as well as molds and yeasts (Table 6) leading to the achievement of a duple refrigerated shelf life (data not presented). Irradiation is among the most effective physical decontamination technologies for inactivating foodborne pathogens and improving the safety of meats  and the effectiveness of such irradiation dose in improving the microbial safety of other meat products was also previously observed [57,58].
3.6. Sensory Properties
On day zero, the sensory evaluation of raw non-irradiated beefburger samples indicated that formulation of burger with olive oil and wheat bran had no adverse effects on the acceptability of their color and appearance as well as their odor. As shown in Table 7, the different formulated non-irradiated burger samples had a similar scores showing no significant differences between their sensory acceptability. The same results further show that irradiation and refrigerated storage (4˚C ± 1˚C) of the different formulated beefburger samples has no significant effect on the acceptability of these sensory attributes as all raw irradiated samples showed a similar high scores compared to the non-irradiated samples except that all nonirradiated burger samples showed a rejection sensory scores for their odor due to the detection of a putrid off-odor on day 12 of their storage (Table 7). Sensory evaluation was also carried out for the cooked burger samples and the sensory traits for cooked samples of the different formulated non-irradiated and irradiated beefburger are given in Table 8. Ratings by the sensory panel showed that there were no significant differences between the acceptability of the control formulated burger and those formulated with olive oil and wheat bran except the fifth formulated burger samples (that prepared with the highest addition of wheat) which showed a significantly lower score for their odor and taste, but were still acceptable, due to the absence of the beef fat. The same observations were recorded for the cooked irradiated burger samples indicating that irradiation of raw beefburger samples at the applied dose had no significant effects on their acceptability after cooking, showing a similar preference scores as compared with the non-irradiated samples (Table 8).These results show that formulation of beefburger with partial replacement of beef fat with olive oil and wheat bran produced acceptable samples compared to the control formulated burger samples with a consistent texture, adequate juiciness and good flavor. Wheat fiber is neutral in taste and help to retain
Table 6. Microbiological quality of the different formulated beefburger samples as affected by irradiation at dose of 3 kGy and refrigerated storage (4˚C ± 1˚C).
Table 7. Sensory attributes for raw non-irradiated and irradiated beefburger formulated with different levels of beef fat, olive oil and wheat bran.
Table 8. Sensory attributes for cooked non-irradiated and irradiated beefburger formulated with different levels of beef fat, olive oil and wheat bran.
moisture and fat leading to producing of a more succulent and juicy meat product . The results of sensory traits for the cooked burger samples agree with those observed for cooking and binding measurements in this study.
It could be concluded that reducing beef fat levels with the addition of olive oil and wheat bran fibers produced a highly acceptable beefburger product with improved nutritional content and cooking and binding properties. Furthermore, irradiation of the resultant beefburger samples at dose of 3 kGy improved their microbiological quality through the complete inactivation of Staphylococcus aureus, Salmonella and enterobacteriaceae, in addition to the significant reduction in the counts of mesophilic and psychrophilic bacteria as well as molds and yeasts, thus, produced a safe beefburger products without any adverse effects on their other quality characteristics.
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