Open Journal of Preventive Medicine
 Vol.2 No.1(2012), Article ID:17358,8 pages DOI:10.4236/ojpm.2012.21001

Fruit intake associated with urinary estrogen metabolites in healthy premenopausal women

Kerryn W. Reding1,2*, Charlotte Atkinson3, Kim C. Westerlind4, Frank Stanczyk5, Erin J. Aiello Bowles6, Mellissa Yong7, Katherine M. Newton6, Johanna W. Lampe2

1School of Nursing, University of Washington, Seattle, USA; *Corresponding Author: kreding@uw.edu

2Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, USA

3Lifecourse Epidemiology & Population Oral Health Research, University of Bristol, Bristol, UK

4Division of Endocrinology, Metabolism and Diabetes, University of Colorado Health Sciences Center, Denver, USA

5Keck School of Medicine, University of Southern California, Los Angeles, USA

6Group Health Research Institute, Seattle, USA

7Center for Observational Research, Amgen, Inc., Thousand Oaks, USA

Received 16 October 2011; revised 27 November 2011; accepted 14 December 2011

Keywords: Estrogen Metabolism; Diet; Botanical Groupings

ABSTRACT

Urinary concentrations of 2:16-hydroxyestrone (2:16-OHE1) approximate concentrations of 2-OHE1 and 16α-OHE1 in breast tissue. As estrogens are purported to be involved in breast cancer development, the 2:16-OHE1 ratio can provide an indication of estrogen metabolite exposure in the breast. With prior studies observing associations between urinary estrogen metabolites and dietary intake of fruits, vegetables, and fiberascertained from food questionnaires, we examined associations between dietary factors ascertained through 3-day food records and urinary 2:16-OHE1 in 191 premenopausal healthy women. Fruit consumption was positively associated with 2:16-OHE1 after adjustment for total energy, ethnicity, body mass index, parity, smoking history, and serum estradiol (p = 0.003). Fruit consumption was positively associated with 2-OHE1 concentrations (p = 0.006), but was not associated with 16α-OHE1 (p = 0.92). The Musaceae botanical grouping (comprised primarily of bananas) was positively associated with the 2:16-OHE1 ratio, and Rosaceae (comprised of citrus fruits) and Musaceae botanical groupings were positively associated with 2-OHE1 (but not 16α-OHE1) concentrations, after adjustment for confounders. Our data suggest that dietary fruit intakeis associated with urinary 2-OHE1 and the 2:16-OHE1 ratio and that breast tissue exposure to estrogen metabolites may thus be influenced by diet.

1. INTRODUCTION

A large accumulation of evidence indicates that hormones, particularly estrogens, play a role in the development of breast cancer [1]. Estrogen metabolism begins with estradiol (E2) and estrone (E1) which can be hydroxylatedby cytochrome P450 enzymes at the 2, 4, and 16 carbon positions to form the 2-, 4- and 16α-hydroxy estrogens, respectively [2,3]. These estrogen metabolites are then inactivated by phase II enzymes, including UDPglucuronosyltransferase (UGT) and glutathione S-transferase (GST), quinonereductase (QR), and sulfotransferase (SULT) enzymes [4-6]. With 2-hydroxyestrone (2-OHE1) being shown to have weak binding to estrogen receptors (ER) and minimal genotoxic effects (in contrast with the 4-hydroxyestrogen [e.g. 4-OHE1 and 4-OHE2]), it has been viewed as the estrogen with the least biologic impact [7,8]. On the other hand 16α-hydroxyestrone (16α- OHE1), has been observed to strongly bind to ER [9,10]. With these contrasting qualities, the 2:16-OHE1 ratio has been proposed to be a marker for estrogenic effects in an individual. However, the link between breast cancer and 2:16-OHE1 has not been consistently observed [11-13]. Despite this, urinary measures of the 2:16-OHE1 ratio approximate the 2:16-OHE1 ratio n the breast-more so than the individual estrogen metabolites [14] indicating that urinary 2:16-OHE1 may provide clues as to the extent of estrogen exposure in the breast.

Research has sought to determine whether modifiable factors influence formation of certain estrogen metabolites. Prior studies indicate that estrogen metabolism may be altered in response to intake of certain dietary constituents, with support coming from laboratory studies, as well as human cross-sectional and intervention studies [6,15-25]. Of the cross-sectional studies investigating associations between a wide range of dietary factors and the 2- and 16α-OHE1 metabolites, a few have reported associations with fruit, vegetable, and coffee consumption, while one reported an association with high fat/low fiber diets [15-18]. Our study sought to investigate associations between urinary 2- and 16α-OHE1 metabolites and dietary factors ascertained through 3-day food records in 191 premenopausal healthy women.

2. METHODS

2.1. Study Design

Participants in the Equol, Breast, and Bone (EBB) study were recruited from the Group Health Cooperative (GHC), a large mixed-model health care system in western Washington State. The methods for this study have been described elsewhere [26]. Briefly, women were eligible if they were premenopausal, aged 40 to 45 years, and had received a screening mammogram at GHC prior to recruitment. Women were ineligible if they were currently using or had used hormone therapy or oral contraceptives for more than one month in the past year; had a personal history of breast cancer, or had shown signs of perimenopause.

After obtaining informed consent, EBB participants completed a health and demographics questionnaire. At the clinic visit, weight, height, waist and hip circumference measurements, percent body fat, and fasting blood and spot urine samples (during days 5 - 9 of their menstrual cycle) were collected. In addition, all participants were asked to complete a 3-day food record (3-DFR) within two weeks of this clinic visit. Participants were given a serving size booklet which contained pictures of commonly consumed foods in different portion sizes, as well as a ruler, a thickness guide, a serving spoon size guide, and tips on how to estimate servings. Dietary intake data from the 3-DFR were analyzed using the Nutrition Data System for Research software by the Nutrition Assessment Shared Resource at the Fred Hutchinson Cancer Research Center using previously described methods [26]. Estimates of daily intake of nutrients, grains, meats and shellfish, egg, dairy, tea and coffee, as well as botanically-defined groupings of fruits and vegetables were obtained.

2.2. Laboratory Analysis

The laboratory analysis for urinary 2-OHE1, 16α-OHE1 and serum E2 have been described previously [27]. Briefly, concentrations of 2-OHE1 and 16α-OHE1 were measured in spot urine samples using a commercially available competitive, solid-phase enzyme-linked immunoassay (ESTRAMET, ImmunaCare Corp., Bethlehem, PA). Serum E2 was quantified by radioimmunoassay after organic solvent extraction and Celite column partition chromatography [28]. Intra-assay and inter-assay coefficients of variation (CV) for 2-OHE1 were 4.4% and 8.8%, respectively; for 16α-OHE1 they were 5.1% and 9.2%, respectively; and for serum E2, the inter-assay CV was 6.2%.

Measurements of urinary creatinine concentrations were based on a kinetic modification of the Jaffe reaction with the use of the Roche Reagent for Creatinine (Roche Diagnostic Systems, Nutley, NJ).

2.3. Statistical Analysis

The ratio of 2:16-OHE1 was computed from the concentrations of 2-OHE1 and 16α-OHE1. When analyzed separately, the 2- and 16-OHE1 values were corrected for creatinine by dividing the estrogen metabolite concentration by the creatinine concentration. Lack of normality was assessed for each continuous variable; serum E2, 2-OHE1, and 16α-OHE1 were skewed, and thus were log-transformed. Quartiles were created for the dietary factors, with the exception of the botanical groupings. For botanical groupings, analysis was limited to those groups that were consumed by at least 30 (≥15%) participants. For those botanical groups, a variable was created that categorized participants as: not consuming the botanical grouping; those consuming less than the median value; and those consuming more than the median value.

For our primary analysis, we tested associations between the 2:16-OHE1 ratio and dietary factors using generalized linear models (GLM) adjusted for potential confounding factors. A confounding factor was included in the GLM if it was statistically significantly associated with the 2:16- OHE1 ratio (as determined using one-way ANOVA) and with dietary factors; all models included confounding factors (including serum E2, ethnicity and smoking history), in addition to total energy. Correlations were also examined between continuous variables using one-way ANOVA to ensure that variables with high correlations were not included in the same model.

To follow up on associations observed for fruit and vegetable consumption, we examined whether associations were observed between botanical groupings and 2:16-OHE1. In addition, to follow up on associations we observed between dietary factors and 2:16-OHE1 we used GLM to estimate the associations between dietary factors and the 2-OHE1 and 16α-OHE1 metabolites separately; these analyses were adjusted for creatinine, and total energy, in addition to confounding factors (ethnicity and smoking history). Benjamini-Hochberg correction was used to adjust for multiple testing in which adjustments were made according to the number of statistical tests for each hypothesis [29]. This analysis was conducted using Stata v. 11.

3. RESULTS

Demographic characteristics of the study population are presented in Table 1. The median age was 42.4 years; median body mass index (BMI) was 25.7 kg/m2. 59.4% of the women had a history of breast feeding and 64.6% were never smokers.

Table 1. Demographic characteristics of the EBB study population.

We observed fruit and vegetable consumption to be positively associated with the 2:16-OHE1 ratio after adjustment for total energy, ethnicity, BMI, parity, smoking history, and serum E2 (Table 2). In models adjusting for total energy only, the following dietary factors were associated with 2:16-OHE1: total sugars (p = 0.01), caffeine (p = 0.03), and refined grains (p = 0.03; data not shown); however in the fully adjusted model, these factors were no longer associated with the 2:16-OHE1 ratio (Table 2). Furthermore, after adjustment for multiple tests only the association between 2:16-OHE1 and fruit consumption remained significant.

We observed two botanical groupings containing fruit to be positively associated with the 2:16-OHE1 ratio in the fully adjusted model (Table 3). Specifically, Rutaceae (e.g., citrus fruits and juices) and Musaceae (comprised primarily of bananas) were positively associated with 2:16- OHE1; however, after adjustment for multiple testing, the association for Rutaceae was no longer significant. Intake of fruit-containing botanical groupings ranged from 21.1% of women consuming for Anacardiaceae (e.g., mangoes) to 85.6% for Rosaceae (e.g., apples, stone fruits, and some berries). 59.8% and 45.9% of women reported consuming Rutaceae and Musaceae, respectively. None of the botanical groupings for vegetables, including Cruciferae (which were consumed by 69.1% of women, although only 8.2% reported eating >1 serving/day), were associated with the 2:16-OHE1 ratio (p-value = 0.94; data not shown).

We next assessed whether fruit or vegetable intake was associated with either 2-OHE1 or 16α-OHE1 separately using the same categorization for fruit and vegetables from Table 2 and for botanical groupings from Table 3. When assessed individually, neither fruit nor vegetable intake, including botanical groupings, were associated with 16α- OHE1 (Table 4). 2-OHE1 was associated with overall fruit consumption and intake of Rutaceae and Musaceae botanical groupings, after adjustment for confounders. While overall fruit consumption and Musacea groupings were positively associated with 2-OHE1, the pattern of association between Ruataceae and 2-OHE1 was less clear. The association between 2-OHE1 and Rutaceae became stronger when confounders were included in the model, indicating an influence of ethnicity and smoking history on the relationships between consumption of foods in the Rutaceae grouping and 2-OHE1.

4. DISCUSSION

We observed fruit consumption to be positively associated with the 2:16-OHE1 ratio among premenopausal women, and this was mostly due to its association with 2-OHE1 concentrations. We also observed that botanical food groupings containing citrus and bananas were associated with 2-OHE1 concentrations. To our knowledge, this is the first study to examine the association between botanical groupings and 2:16-OHE1 in premenopausal women.

Our findings are biologically plausible given that dietary factors can influence phase I and phase II enzymes, and these enzymes act upon estrogen metabolites. Estroneishydroxylated into 2-, 4-, and 16α-OHE1 by cytochrome P450 (CYP) enzymes. Specifically, CYP1A1 and CYP1B1 predominantly hydroxylate estrogens into 2- and 4-OHE1, respectively, while CYP1A2 and CYP3A4 hydroxylate estradiol into 16α-OHE1 [7]. Next, phase II enzymescan inactivate hydroxy estrogens, rendering them more easily excreted. Laboratory studies have demonstrated

Table 2. Associations of nutrient and food groups with the urinary 2:16 hydroxy-estrone ratio.

Table 3. Association between urinary 2:16-OHE1 ratio and selected botanical groups.

Table 4. Associations between 2-hydroxyestrone, 16α-hydroxyestrone, and selected dietary factors.

support for a role of diet in estrogen metabolism, showing that dietary factors can induce phase I enzymes involved in estrogen metabolism [6,19-25]. For example, several components of cruciferous vegetables, including isothiocyanatesand indole-3-carbinol (I3C), have been demonstrated in vitro to influence CYP enzymes [23,24]. More specifically, I3C binds to aryl-hydrocarbon recaptor (AhR), which binds to the xenobiotic response element on the promoter of CYP1A1, thereby inducing expression of CYP1A1 [23], and leading to increased 2-OHE1 concentrations. In addition, quercetin, a flavonoid found widely in fruit, vegetables, and grains (including citrus fruits and leafy greens), has been shown to also bind to AhR and induce CYP1A1 expression [20]. Furthermore, quercetin was observed to decrease CYP1A2 activity in vivo in both animal and human studies [6,21,22], which would conceivably lead to decreased 16α-OHE1 concentrations.

Numerous phytochemicals, including quercetin and other flavonoids (including naringenin found in citrus fruits), have also been shown to influence the activity of phase II enzymes, including UGT, GST, QR, and SULT enzymes [4-6]. These relationships are complex, as flavonoids are demonstrated to induce some phase II enzymes, including multiple UGT enzymes and QR, while inhibiting others, such as SULT, but overall the influence of flavonoids on phase IIenzymes is consistent with chemoprevention.

Prior observational studies have reported on associations between dietary factors from questionnaire data and 2-OHE1 and 16α-OHE1 concentrations [15-18]. Reports include an inverse association between vegetable intake and 16α-OHE1 [15], a positive association between foods rich in hydroxybenzoic acids (e.g. berries) and 2-OHE1 [15], an inverse association between low fat/high fiber and 16α-OHE1 [17], and a positive association between coffee consumption and 2:16-OHE1 [18], all of which favorably alter the 2:16α-OHE1 ratio [7,8,17]. However, while most of these cross-sectional studies investigated similar dietary factors in relation to 2- and 16α-OHE1, there were not entirely consistent findings across these studies.

Further support for the impact of dietary intake on estrogen metabolites come from human feeding and intervention studies. Feeding studies and interventions have also observed increased consumption of isoflavones (genistein and daidzein), flaxseed, I3C supplementation, and lowfat diets to be associated with increased 2-OHE1 concentrations and the 2:16-OHE1 ratio in women [30-33], findings that are consistent with the in vitro studies discussed above. With flaxseed and berries being a good source of lignans [34], and our finding that fruit was associated with 2:16-OHE1, we investigated the potential for berries (botanical groupings Ericaceae and Rosaceae) to be associated with 2:16-OHE1. Although we did not see evidence of an association between Ericaceae and Rosaceae and 2:16-OHE1 it is possible that the levels of berry consumption were too low to detect associations and that lignans may actually contribute in part to fruit consumption being associated with 2:16-OHE1. Our study observed fruit intake to be associated with the 2:16-OHE1 ratio. While it is possible that these findings are spurious, the association between 2:16-OHE1 and fruit intake and the Musaceaegrouping remained significant after the correction for multiple tests. Furthermore, in the context of the aforementioned support from prior animal and human studies [15,16,20-22], this finding may have validity. The observed association between vegetables and 2:16-OHE1 should be interpreted with caution as these associations were not significant after correction for multiple testing, and as a result may be more likely due to chance.

Limitations of our study include the use of participant recall for dietary intake. While dietary assessment tools in general may misclassify dietary intake, a 3-day food record (which our study used) arguably has higher validity than a food frequency questionnaire, particularly for major food groups [35-37]. Another limitation centers on the issue of generalizability. The women in our study representa more health-conscious segment of the general population, with for example a mean BMI of 25.7 in our study and only 4% of participants being current smokers. Thus their dietary intake may not be representative of women from the US population as a whole. However, these characteristics may enhance the internal validity because confounding factors, such as cigarette smoking, are minimized in this rather homogeneous population. An additional limitation involves the use of a single spot urine collection for assessment of the 2- and 16α-OHE1 concentrations. First, previous studies have indicated that there was no difference between a spot urine and a 24-hour urine for assessment of 2:16-OHE1 [38], and that urinary 2:16-OHE1 is correlated with plasma 2:16-OHE1 (r2 = 0.83 among nonOC users whose urine samples are collected mid-cycle, which matches our study’s protocol) [39]. However, despite the high reproducibility of the assays for the 2- and 16α-OHE1 as indicated by the low CVs, Williams et al. estimated that 5 collections would be ideal in order to capture the variability in urinary hydroxy estrogens [40]. Lastly, as with all cross-sectional studies, we were unable to assess the temporality of the diet and 2:16-OHE1 association. However, a strength of cross-sectional studies are their ability to investigate a variety of food groups associated with 2:16-OHE1, and in the case of diet and 2:16-OHE1 multiple diet interventions have previously demonstrated that diet precedes changes in the 2:16-OHE1 [30-33]. Furthermore, investigation of associations with botanical groupings in particular allowed for an examination of specific sources of phytochemicals within the diet.

Our study adds to the large body of literature that indicatesdietary intake is associated with 2:16-OHE1 [6,15-25] in premenopausal women. With urinary 2:16-OHE1 representing the 2:16-OHE1 ratio in the breast [14], this line of research may have implications for modifiable factors related to breast cancer. However, while we observed that fruit consumption, including the botanical grouping Musaceae, was associated with increasing 2- OHE1, these results would need to be replicated in larger, more generalizable studies of premenopausal women before definitive conclusions can be drawn. Such studies would ideally be designed to report on botanical groupings in relation to urinary estrogen metabolites, including the 2- and 16α-OHE1 in order to shed light on the particular aspects of the diet that are associated with estrogen metabolism among premenopausal women.

5. ACKNOWLEDGEMENTS

We would like to thank the research participants for their involvment in this project, and thank Kelly Ehrlich, Kathy Plant, and the Group Health Research Institute for screening interviews, clinic visits, and study coordination. This study was supported by NIH grant R01CA97366 and U01CA63731; KWR was supported by NIH grant K99NR012232.

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